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The map label for this gene is rnhA

Identifier: 83309861

GI number: 83309861

Start: 819075

End: 819539

Strand: Reverse

Name: rnhA

Synonym: amb0762

Alternate gene names: 83309861

Gene position: 819539-819075 (Counterclockwise)

Preceding gene: 83309862

Following gene: 83309856

Centisome position: 16.5

GC content: 66.24

Gene sequence:

>465_bases
ATGAGCGAGACAGCTCCCAAGCCCGAGACCGTCGAGATCTATACCGACGGCGCCTGTTCCGGCAATCCCGGCCCCGGCGG
CTGGGGCGCCATCCTGCGCTTCAAGGGCATCGAGAAGGAGTTGAAGGGCGGCGAAAGCCCGACCACCAACAACCGCATGG
AAATGATGGCGGTGCTGGTGGCGCTCAACACCCTGACGCGATCCTGCGCCGTCGACGTCTACACCGACAGCGAATACGTC
AAGAAGGGCATGACCGAATGGCTGCGCGGCTGGAAGGCGCGGGGCTGGAAGACCGCCGACAAGAAGCCGGTGAAGAACGA
CGACCTGTGGAAGGCCCTGGACGAGGCCGCCGCCCGGCACAAGGTGTCGTGGCACTGGGTCAAGGGCCATGCCGGCCATC
CCGAGAACGAGCGCGCCGACGCCCTGGCCCGCGAAGGCATCGCGGACCTGCGCGCCAGAACATAA

Upstream 100 bases:

>100_bases
CCCCGCCCGGCGCCATGGTCAAGCGCAAGGACCCCATGGAATATTACCGCAAGCTCCGCTTCCACCAGGGCGTCACCGGC
CCGGGCCAGTACGGCATCGA

Downstream 100 bases:

>100_bases
TTCCGGCGAGCCCCCACTTTCAATTTGCAAATGAAAGAGAGGGTTGGAGGCATCGCCTCCAACCGGGGACCGGGGCGGGA
GCCCCGCCTTCAAGCCCTCC

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSETAPKPETVEIYTDGACSGNPGPGGWGAILRFKGIEKELKGGESPTTNNRMEMMAVLVALNTLTRSCAVDVYTDSEYV
KKGMTEWLRGWKARGWKTADKKPVKNDDLWKALDEAAARHKVSWHWVKGHAGHPENERADALAREGIADLRART

Sequences:

>Translated_154_residues
MSETAPKPETVEIYTDGACSGNPGPGGWGAILRFKGIEKELKGGESPTTNNRMEMMAVLVALNTLTRSCAVDVYTDSEYV
KKGMTEWLRGWKARGWKTADKKPVKNDDLWKALDEAAARHKVSWHWVKGHAGHPENERADALAREGIADLRART
>Mature_153_residues
SETAPKPETVEIYTDGACSGNPGPGGWGAILRFKGIEKELKGGESPTTNNRMEMMAVLVALNTLTRSCAVDVYTDSEYVK
KGMTEWLRGWKARGWKTADKKPVKNDDLWKALDEAAARHKVSWHWVKGHAGHPENERADALAREGIADLRART

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=143, Percent_Identity=35.6643356643357, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786408, Length=134, Percent_Identity=64.1791044776119, Blast_Score=193, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI115532542, Length=139, Percent_Identity=38.1294964028777, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137476, Length=148, Percent_Identity=34.4594594594595, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45552509, Length=148, Percent_Identity=34.4594594594595, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_MAGSA (Q2W9A9)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_420125.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q2W9A9
- SMR:   Q2W9A9
- STRING:   Q2W9A9
- GeneID:   3806329
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb0762
- NMPDR:   fig|342108.5.peg.716
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q2W9A9
- BioCyc:   MMAG342108:AMB0762-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17050; Mature: 16919

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETAPKPETVEIYTDGACSGNPGPGGWGAILRFKGIEKELKGGESPTTNNRMEMMAVLV
CCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHH
ALNTLTRSCAVDVYTDSEYVKKGMTEWLRGWKARGWKTADKKPVKNDDLWKALDEAAARH
HHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHC
KVSWHWVKGHAGHPENERADALAREGIADLRART
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETAPKPETVEIYTDGACSGNPGPGGWGAILRFKGIEKELKGGESPTTNNRMEMMAVLV
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHH
ALNTLTRSCAVDVYTDSEYVKKGMTEWLRGWKARGWKTADKKPVKNDDLWKALDEAAARH
HHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHC
KVSWHWVKGHAGHPENERADALAREGIADLRART
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA