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The map label for this gene is ydaP [H]

Identifier: 82752122

GI number: 82752122

Start: 2548674

End: 2550413

Strand: Reverse

Name: ydaP [H]

Synonym: SAB2413c

Alternate gene names: 82752122

Gene position: 2550413-2548674 (Counterclockwise)

Preceding gene: 82752123

Following gene: 82752121

Centisome position: 92.99

GC content: 34.48

Gene sequence:

>1740_bases
ATGGCAAAAATAAAAGCAAATGAAGCATTAGTTAAAGCATTACAAGCATGGGATATAGATCACTTGTATGGTATTCCAGG
AGACTCAATCGACGCAGTTGTCGATAGTTTACGTACAGTGAGAGATCAATTTAAATTTTATCATGTACGTCATGAAGAAG
TAGCAAGCTTAGCGGCTGCTGGTTACACAAAATTAACTGGTAAAATCGGTGTGGCATTAAGTATCGGTGGTCCTGGTTTA
ATTCATTTATTAAATGGTATGTACGATGCCAAAATGGATAATGTACCGCAATTAATATTATCTGGACAAACGAATAGTAC
AGCACTTGGAACGAAAGCATTTCAAGAAACAAATTTACAAAAATTATGTGAAGATGTAGCCGTTTATAATCACCAAATTG
AAAAAGGTGACAATGTGTTTGAAATCGTTAACGAAGCAATTCGTACGGCATATGAACAAAAAGGTGTCGCTGTTGTTATT
TGTCCTAACGACTTATTAACTGAAAAAATTAAAGATACAACGAATAAACCAGTAGATACATCAAGACCAATAGTAGTATC
ACCAAAATATAAAGACATCAAAAAAGCGGTTAAACTAATTAATAAAAGTAAAAAGCCTGTCATGTTAATTGGTGTAGGCG
CGAAACATGCGAAAGATGAGCTACGTGAATTTATTGAAATGGCTAAAATTCCTGTCATTCATTCATTACCAGCTAAAACA
ATCTTGCCGGATGATCATCCATATAGTATCGGTAACTTAGGTAAAATCGGTACCAAAACATCTTATCAAACAATGCAGGA
AGCGGATTTATTAATTATGGTTGGTACAAACTATCCATATGTGGATTACTTACCTAAGAAAAATATTAAAGCCATTCAAA
TTGACACAAATCCTAAAAATATCGGACATCGTTTCAATATTAATGTGGGAATTGTTGGAGATAGTAAAATTGCGTTGCAT
CAGTTAACTGAAAATATTAAACATGTTGCTGAAAGACCATTCTTAAACAAAACGTTAGAACGTAAAGCGGTTTGGGATAA
ATGGATGGAACAAGATAAAAATAATAATAGTAAACCATTACGTCCAGAACGATTAATGGCATCAATCAATAAATTTATTA
AAGATGATGCAGTGATTTCAGCAGATGTAGGTACAGCAACAGTTTGGTCAACTCGATACTTAAACCTTGGTTTAAATAAC
AAGTTCATCATTTCAAGTTGGTTAGGTACAATGGGTTGCGGTCTTCCAGGTGCAATGGCATCAAAAATTGCATATCCAAA
TAGACAAGCCATCGCAATTGCTGGTGACGGTGCATTCCAAATGGTGATGCAGGACTTTGCAACAGCTGTACAATATGATT
TACCTTTAACAGTGTTTGTTCTAAATAATAAGCAATTAGCGTTTATTAAATATGAACAACAAGCTGCTGGTGAATTAGAA
TATGCGGTTGATTTCTCTGATATGGATCATGCCAAATTTGCTGAAGCAGCAGGTGGTAAAGGTTATACAATTAAAAGTGC
TAGCGAAGTAGATGCTATTGTAGAAGAGGCACTAGCACAAGATGTACCAACAATTGTAGATGTTTATGTTGATCCTAATG
CTGCGCCATTACCAGGTAAAATTGTAAATGAAGAAGCGCTTGGTTATGGTAAATGGGCATTTAGATCTATTACTGAAGAT
AAATATTTAGATTTAGATCAAATTCCGCCTATTTCAGTTGCAGCAAAACGTTTCTTATAA

Upstream 100 bases:

>100_bases
GATTTTAACTGCAGTTATTAGTTCAGTGTTAATACCTGTTCTGATCTTACTATTTTACTAATTAGAAAAAGACACTAAGA
AAGGGAGGCTATTAGTAATA

Downstream 100 bases:

>100_bases
CTGATTGAAAGGTTATCACAATTGAATTGAACTATAAAAACGGTAATTTCTATTTCAACAAAATGGGAATTGCCGTTTTG
TTTATTTATCACAAATGATT

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGL
IHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
CPNDLLTEKIKDTTNKPVDTSRPIVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALH
QLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGLNN
KFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITED
KYLDLDQIPPISVAAKRFL

Sequences:

>Translated_579_residues
MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGL
IHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
CPNDLLTEKIKDTTNKPVDTSRPIVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALH
QLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGLNN
KFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITED
KYLDLDQIPPISVAAKRFL
>Mature_578_residues
AKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLI
HLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVIC
PNDLLTEKIKDTTNKPVDTSRPIVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKTI
LPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQ
LTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGLNNK
FIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELEY
AVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDK
YLDLDQIPPISVAAKRFL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=22.7758007117438, Blast_Score=169, Evalue=6e-42,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=33.2730560578662, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=29.1814946619217, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1790104, Length=545, Percent_Identity=26.605504587156, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=25.4867256637168, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=24.5519713261649, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=22.5, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=22.5, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=581, Percent_Identity=24.9569707401033, Blast_Score=129, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=28.6225402504472, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.468085106383, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321524, Length=559, Percent_Identity=23.613595706619, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320588, Length=532, Percent_Identity=22.3684210526316, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=527, Percent_Identity=21.2523719165085, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=25.886524822695, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 63828; Mature: 63697

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAA
CCCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHC
GYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQ
CCCEECCCEEEEEEECCCHHHHHHCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCHH
KLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVICPNDLLTEKIKDTTNKPVDT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCC
SRPIVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
CCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCC
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKN
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCC
IGHRFNINVGIVGDSKIALHQLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPL
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGLNNKFIISSWLGTMGCGLPGAMA
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCCCCEEHHHHHHHHCCCCCHHHH
SKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHEECCCEEEEEECCCEEEEEEECCCCCCCEE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGK
EEEECCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
IVNEEALGYGKWAFRSITEDKYLDLDQIPPISVAAKRFL
CCCHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
AKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQFKFYHVRHEEVASLAAA
CCCCHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHC
GYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSGQTNSTALGTKAFQETNLQ
CCCEECCCEEEEEEECCCHHHHHHCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCHH
KLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVICPNDLLTEKIKDTTNKPVDT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCCCC
SRPIVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAKDELREFIEMAKIPVIHSLPAKT
CCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCC
ILPDDHPYSIGNLGKIGTKTSYQTMQEADLLIMVGTNYPYVDYLPKKNIKAIQIDTNPKN
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCC
IGHRFNINVGIVGDSKIALHQLTENIKHVAERPFLNKTLERKAVWDKWMEQDKNNNSKPL
CCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPERLMASINKFIKDDAVISADVGTATVWSTRYLNLGLNNKFIISSWLGTMGCGLPGAMA
CHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEECCCCCEEHHHHHHHHCCCCCHHHH
SKIAYPNRQAIAIAGDGAFQMVMQDFATAVQYDLPLTVFVLNNKQLAFIKYEQQAAGELE
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHEECCCEEEEEECCCEEEEEEECCCCCCCEE
YAVDFSDMDHAKFAEAAGGKGYTIKSASEVDAIVEEALAQDVPTIVDVYVDPNAAPLPGK
EEEECCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
IVNEEALGYGKWAFRSITEDKYLDLDQIPPISVAAKRFL
CCCHHHCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]