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The map label for this gene is purD

Identifier: 82750685

GI number: 82750685

Start: 1020912

End: 1022159

Strand: Direct

Name: purD

Synonym: SAB0941

Alternate gene names: 82750685

Gene position: 1020912-1022159 (Clockwise)

Preceding gene: 82750684

Following gene: 82750689

Centisome position: 37.23

GC content: 35.82

Gene sequence:

>1248_bases
ATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACA
AGTGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTGATCATCAAG
GGATACTAGATTTTGCTAAACAGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAACCGCTAATTGATGGATTAGCA
GACATTTTACGAGCGAATGGTTTCAAAGTGTTTGGTCCAAATAAGCAAGCAGCTCAAATCGAAGGCTCAAAATTATTTGC
TAAAAAGATAATGGAAAAATATAATATTCCAACTGCTGATTATAAAGAAGTTGAGCGAAAAAAGGATGCTTTAACATATA
TTGAGAACTGTGAATTGCCCGTTGTTGTCAAGAAAGATGGGTTAGCTGCTGGGAAAGGCGTTATTATTGCAGATACTATT
GAAGCAGCCAGAAGTGCTATTGAGATTATGTATGGTGATGAAGAAGAAGGTACTGTTGTATTTGAAACGTTTTTAGAAGG
TGAAGAGTTCTCGCTAATGACATTTGTAAATGGTGATTTAGCAGTTCCTTTCGACTGTATTGCACAAGATCATAAACGCG
CATTTGATCATGATGAAGGACCAAATACTGGTGGTATGGGGGCTTATTGTCCAGTACCACATATTAGTGACGATGTTTTA
AAACTTACAAATGAAACGATTGCACAACCCATTGCAAAGGCAATGCTTAATGAAGGTTATCAATTCTTCGGTGTATTATA
CATTGGTGCTATTTTAACTAAAGATGGTCCAAAAGTAATTGAATTTAATGCCCGTTTTGGTGATCCTGAAGCTCAAGTAT
TATTAAGTCGCATGGAAAGTGATTTAATGCAGCATATTATTGATTTAGATGAAGGAAACCGTACTGAATTCAAATGGAAA
AATGAATCTATTGTAGGGGTCATGTTGGCATCAAAAGGATATCCTGATGCATATGAAAAAGGGCATAAAGTAAGTGGTTT
TGATTTAAATGAAAACTATTTTGTTAGTGGATTAAAGAAGCAAGGTGATACCTTTGTTACTTCAGGTGGTAGAGTTATAC
TTGCCATCGGAAGAGGTGACAATGTACAAGATGCACAGCGAGACGCATACGAAAAAGTATCTCAAATACAAAGTGACCAT
TTATTCTATCGTCATGACATTGCGAATAAAGCACTACAACTTAAATAA

Upstream 100 bases:

>100_bases
AGATCAAGATTCAATTGATATGGCTAATAAACATGGTATTGCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAAT
TTTAAAGGGGCGTAACATAA

Downstream 100 bases:

>100_bases
GTATATTTAAAAATACTAAGATTAGCTATGAACGAATCTATAACGATAGATTTTTTCATAGCTTTTTTAGTTGTAGAGTC
TAGGACATTGATTTCTGTAC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGNRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGRGDNVQDAQRDAYEKVSQIQSDH
LFYRHDIANKALQLK

Sequences:

>Translated_415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGNRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGRGDNVQDAQRDAYEKVSQIQSDH
LFYRHDIANKALQLK
>Mature_415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGNRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGRGDNVQDAQRDAYEKVSQIQSDH
LFYRHDIANKALQLK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=43.1924882629108, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=43.1924882629108, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=43.1924882629108, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=43.1924882629108, Blast_Score=330, Evalue=2e-90,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=44.1588785046729, Blast_Score=338, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=39.0070921985816, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321203, Length=443, Percent_Identity=37.9232505643341, Blast_Score=279, Evalue=5e-76,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=40.2777777777778, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=40.2777777777778, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45822; Mature: 45822

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISESDHQGILDFAK
CCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHH
QQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTAD
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
YKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTIEAARSAIEIMYGDEEEGTVV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEEECCCCCCEEE
FETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLMQHIIDLDEGNRTEFKWKNESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKK
HHHHHHHCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCEEECHHC
QGDTFVTSGGRVILAIGRGDNVQDAQRDAYEKVSQIQSDHLFYRHDIANKALQLK
CCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHCCCEECC
>Mature Secondary Structure
MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVHTEISESDHQGILDFAK
CCEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHH
QQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEGSKLFAKKIMEKYNIPTAD
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCC
YKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTIEAARSAIEIMYGDEEEGTVV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEEECCCCCCEEE
FETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLMQHIIDLDEGNRTEFKWKNESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKK
HHHHHHHCCCCCCCCEEEECCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCEEECHHC
QGDTFVTSGGRVILAIGRGDNVQDAQRDAYEKVSQIQSDHLFYRHDIANKALQLK
CCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA