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The map label for this gene is purH

Identifier: 82750684

GI number: 82750684

Start: 1019412

End: 1020890

Strand: Direct

Name: purH

Synonym: SAB0940

Alternate gene names: 82750684

Gene position: 1019412-1020890 (Clockwise)

Preceding gene: 82750683

Following gene: 82750685

Centisome position: 37.17

GC content: 34.69

Gene sequence:

>1479_bases
ATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCGTTAACGCAATTAAATTATGA
ATTATATTCAACAGGTGGTACTAAACGTATATTAGATGAAGCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACACATT
TTCCAGAAATAATGGATGGCCGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAACCG
CAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTTAATTTATATCCATTCCAACAAAC
TGTTGCAAACCCTGATGTGACGATGGACGAAGCAATTGAAAATATTGATATTGGTGGTCCAACAATGTTACGTGCTGCAG
CTAAAAACTATAAACATGTAACAACAATTGTACATCCTGCAGATTATCAAGAAGTATTGACGCGATTAAGAAACGATTCG
TTAGATGAGTCATATAGACAATCATTAATGATCAAAGTTTTTGAGCATACTGCAGAATATGATGAAGCGATTGTACGTTT
CTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAAAATCCACAACAATCAGCGTATTTTGTGAGAACTTCGAATGCTA
AGCACACGATTGCAGGCGCTAAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACACTA
GCTTTAGTTAAAAAGTTTGATACACCTGCTGCAGTTGCGGTTAAACACATGAATCCATGTGGTGTTGGTATCGGTGACAC
GATAGAACAAGCATTTCAACATGCGTATGAAGCGGATAGTCAATCAATATTTGGTGGAATTGTTGCATTAAACCGAGCTG
TAACACCTGAGTTAGCAGAGCAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCATTA
GATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGATAGTAACGAAGAAGAGTTTGTTTC
AGTATCTGGCGGATATTTAGTTCAAGATAAAGACAATTATGTGGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAGTTG
CACCTACTGATGAACAATGGGAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATTTTA
AGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCTGCTAAAATTGCGTTAGAGAGAGC
TATTGAAATCAATGATCATGTAGCGTTAGTATCTGATGGATTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCACAAC
ATGGTATAAAGGCAATTATCCAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGTATT
GCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA

Upstream 100 bases:

>100_bases
ACGATTCAAAAGAACAATTAGAAGAGAAAGTAAAAAAATTGGAATATGAGTTATATCCAAGTGTTATTGCTAAAATTGTA
AAATAAGGAGTATGTATGTG

Downstream 100 bases:

>100_bases
TTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAA
TCAATCGAATCTAGTTAAAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKP
QHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEAL
DILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIIL
SNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
AMVVTGTRHFKH

Sequences:

>Translated_492_residues
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKP
QHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEAL
DILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIIL
SNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
AMVVTGTRHFKH
>Mature_492_residues
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKP
QHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDS
LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEAL
DILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIIL
SNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
AMVVTGTRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=461, Percent_Identity=38.8286334056399, Blast_Score=272, Evalue=6e-73,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=47.2380952380952, Blast_Score=453, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71985564, Length=466, Percent_Identity=34.1201716738197, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=460, Percent_Identity=37.3913043478261, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6323768, Length=469, Percent_Identity=36.4605543710021, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649832, Length=463, Percent_Identity=37.1490280777538, Blast_Score=258, Evalue=9e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 54319; Mature: 54319

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDG
CCCCEEEECCCCCHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHCHHHHCC
RVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIE
CHHEECHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHH
NIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDSLDESYRQSLMIKVFEHTAEY
HCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
HHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCEECCCHHHHCCCCCCCCCCCCHHHHH
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAE
HHHHHCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
QLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNY
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCEEEECCCCE
VVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAA
ECCHHHHHHHHHCCCCCHHHHHHEECEEECCCCCCCEEEEECCCEEEEECCCCCCCCHHH
KIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
HHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCE
AMVVTGTRHFKH
EEEEECCCCCCC
>Mature Secondary Structure
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDG
CCCCEEEECCCCCHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHCHHHHCC
RVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIE
CHHEECHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHH
NIDIGGPTMLRAAAKNYKHVTTIVHPADYQEVLTRLRNDSLDESYRQSLMIKVFEHTAEY
HCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
HHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCEECCCHHHHCCCCCCCCCCCCHHHHH
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAE
HHHHHCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
QLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNY
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCEEEECCCCE
VVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAA
ECCHHHHHHHHHCCCCCHHHHHHEECEEECCCCCCCEEEEECCCEEEEECCCCCCCCHHH
KIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
HHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCE
AMVVTGTRHFKH
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA