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The map label for this gene is murD

Identifier: 82703611

GI number: 82703611

Start: 2851469

End: 2852890

Strand: Reverse

Name: murD

Synonym: Nmul_A2496

Alternate gene names: 82703611

Gene position: 2852890-2851469 (Counterclockwise)

Preceding gene: 82703612

Following gene: 82703610

Centisome position: 89.59

GC content: 58.51

Gene sequence:

>1422_bases
ATGAACCTGCAAGGCAGAACTGTTCTGGTACTGGGCCTCGGAGAGACCGGCCTTTCCATGGCAAAATGGTTGTTTCGGCG
CGGAGCGCTGGTGCGAGCAGCTGATACCCGGAACGAGCCACCGGCCATGAGGGCGTTTAACAGCCTGCTGCCGCAGGCGG
AGGTTTTTACAGGTTCCCTGGCCGGCAGAGCCTTTTACGGCGTGGATTTAATCGCAATCAGTCCCGGTCTGCCCCTGTCG
GAGCCTCTTGTGCAGCAGGCGCTGAAAGAAGGCATCCCGGTGGTCGGGGATATGGAGCTTTTTGCCTGCGCAGTCGGAGG
CACGGGGGGGGGCATGCCAAAACTCGTCGGGATTACCGGCTCCAATGGCAAGACCACGGTGACCGCCATGACAGGGGCAA
TGTTAAAAAAAGCGGGGTGGGACGTCGAAGTAGCGGGAAATATCGGTCCTGCGGTACTGGATGCACTGATGCGCCGGGAG
GATACTGGAAAAATGCCCCAGGCGTGGGTGCTGGAGCTTTCCAGCTTCCAGCTGGAAACCACGAAGAGTCTGGGACTGGA
TGCAGCCGCAGTGCTGAATGTAAGCGAAGATCATCTTGACCGCTACGCAGGCATGCAGGAATACGCTGCTGCTAAGGCCC
GGATATTTCTTGGTGACAGCGAGGGTGTGCAAATCCTGAATGGCGATGATCCTGTCGTCCGGCAAATGACGTTAGCGGGT
AGACCTCACGTCACTTTCAGTCTTGCTACGCCACAAAGCGCCGACGATTTCGGCTTGCTTCGCGAAGGCGGAGACACCTG
GCTGATGCAGGGAGACACGCGTCTCATGGACGCGCGTGAACTCGCCATTACTGGCCGGCACAATTGCGCAAATGCACTGG
CGGCACTGGCGCTATGCCGCGCCCTGGCCGTCCCGTTCGAGCCTTTGTTACAGGCGCTCCGCGAATTCAGGGGCTTGCCC
CATCGAGTTGAAAAAGTGGCGGCTTTCAGTGGTATTACCTTCTACGACGATTCAAAAGGTACTAATGTGGGTGCAACGGT
GGCTGCTTTGAAAGGTCTGGGGCAACCCGTGGTTTTGATTGCCGGCGGAGATGGGAAGGCGCAGAATTTTTCACCCCTGG
CTGCCCCGATAGGCGAGCATGGGCGCGCGGTTGTTCTCATCGGGCGGGATGCAGAAAAAATCGCAGTGGCGATAAATGAG
TGCGGAGTGCCTCTGCATCGTGCTCAAACGATGGAAGAGGCCGTGCGGAAAAGTTTTCAGCTGGCGCGGGAGGGCGATGC
AGTCCTGATGTCGCCCGCGTGCGCAAGCTTCGATATGTTCGACAACTATGTGCATCGGGCGGAGGCGTTCGTTGCGGCGG
TAAGAAGCATGCAGGCCGCACGTGCAGGCGGTAATTCAGCTGGGGTTGCAGGACGACATTGA

Upstream 100 bases:

>100_bases
GGCTGGAAGGAAAACCAGGTAGTGGTCCGGTTCTGGATCATTACCATGATGCTAGTGCTGTTCGGTTTGTCTTCCCTGAA
GCTGAGATAAAGTTGAAATC

Downstream 100 bases:

>100_bases
TCAGATGATTTATCAGTACGGTACCGGAAAGCAGAAAGTTCTGCCTGCGGATTTCGACCAGGCCCTGATCTGGACAGCAA
TACTCCTGCTGAGCCTTGGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNLQGRTVLVLGLGETGLSMAKWLFRRGALVRAADTRNEPPAMRAFNSLLPQAEVFTGSLAGRAFYGVDLIAISPGLPLS
EPLVQQALKEGIPVVGDMELFACAVGGTGGGMPKLVGITGSNGKTTVTAMTGAMLKKAGWDVEVAGNIGPAVLDALMRRE
DTGKMPQAWVLELSSFQLETTKSLGLDAAAVLNVSEDHLDRYAGMQEYAAAKARIFLGDSEGVQILNGDDPVVRQMTLAG
RPHVTFSLATPQSADDFGLLREGGDTWLMQGDTRLMDARELAITGRHNCANALAALALCRALAVPFEPLLQALREFRGLP
HRVEKVAAFSGITFYDDSKGTNVGATVAALKGLGQPVVLIAGGDGKAQNFSPLAAPIGEHGRAVVLIGRDAEKIAVAINE
CGVPLHRAQTMEEAVRKSFQLAREGDAVLMSPACASFDMFDNYVHRAEAFVAAVRSMQAARAGGNSAGVAGRH

Sequences:

>Translated_473_residues
MNLQGRTVLVLGLGETGLSMAKWLFRRGALVRAADTRNEPPAMRAFNSLLPQAEVFTGSLAGRAFYGVDLIAISPGLPLS
EPLVQQALKEGIPVVGDMELFACAVGGTGGGMPKLVGITGSNGKTTVTAMTGAMLKKAGWDVEVAGNIGPAVLDALMRRE
DTGKMPQAWVLELSSFQLETTKSLGLDAAAVLNVSEDHLDRYAGMQEYAAAKARIFLGDSEGVQILNGDDPVVRQMTLAG
RPHVTFSLATPQSADDFGLLREGGDTWLMQGDTRLMDARELAITGRHNCANALAALALCRALAVPFEPLLQALREFRGLP
HRVEKVAAFSGITFYDDSKGTNVGATVAALKGLGQPVVLIAGGDGKAQNFSPLAAPIGEHGRAVVLIGRDAEKIAVAINE
CGVPLHRAQTMEEAVRKSFQLAREGDAVLMSPACASFDMFDNYVHRAEAFVAAVRSMQAARAGGNSAGVAGRH
>Mature_473_residues
MNLQGRTVLVLGLGETGLSMAKWLFRRGALVRAADTRNEPPAMRAFNSLLPQAEVFTGSLAGRAFYGVDLIAISPGLPLS
EPLVQQALKEGIPVVGDMELFACAVGGTGGGMPKLVGITGSNGKTTVTAMTGAMLKKAGWDVEVAGNIGPAVLDALMRRE
DTGKMPQAWVLELSSFQLETTKSLGLDAAAVLNVSEDHLDRYAGMQEYAAAKARIFLGDSEGVQILNGDDPVVRQMTLAG
RPHVTFSLATPQSADDFGLLREGGDTWLMQGDTRLMDARELAITGRHNCANALAALALCRALAVPFEPLLQALREFRGLP
HRVEKVAAFSGITFYDDSKGTNVGATVAALKGLGQPVVLIAGGDGKAQNFSPLAAPIGEHGRAVVLIGRDAEKIAVAINE
CGVPLHRAQTMEEAVRKSFQLAREGDAVLMSPACASFDMFDNYVHRAEAFVAAVRSMQAARAGGNSAGVAGRH

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=453, Percent_Identity=42.1633554083885, Blast_Score=289, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_NITMU (Q2Y636)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413177.1
- ProteinModelPortal:   Q2Y636
- STRING:   Q2Y636
- GeneID:   3786755
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2496
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- PhylomeDB:   Q2Y636
- ProtClustDB:   PRK02006
- BioCyc:   NMUL323848:NMUL_A2496-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49803; Mature: 49803

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQGRTVLVLGLGETGLSMAKWLFRRGALVRAADTRNEPPAMRAFNSLLPQAEVFTGSL
CCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHCCC
AGRAFYGVDLIAISPGLPLSEPLVQQALKEGIPVVGDMELFACAVGGTGGGMPKLVGITG
CCCEEECEEEEEECCCCCCCHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCCEEEEEEC
SNGKTTVTAMTGAMLKKAGWDVEVAGNIGPAVLDALMRREDTGKMPQAWVLELSSFQLET
CCCCEEEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCHHHHEEHHCCEEEH
TKSLGLDAAAVLNVSEDHLDRYAGMQEYAAAKARIFLGDSEGVQILNGDDPVVRQMTLAG
HHHCCCCCCEEECCCHHHHHHHCCHHHHHHCEEEEEEECCCCEEEECCCCHHHHHHHHCC
RPHVTFSLATPQSADDFGLLREGGDTWLMQGDTRLMDARELAITGRHNCANALAALALCR
CCCEEEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCHHEEEECCCHHHHHHHHHHHHH
ALAVPFEPLLQALREFRGLPHRVEKVAAFSGITFYDDSKGTNVGATVAALKGLGQPVVLI
HHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEE
AGGDGKAQNFSPLAAPIGEHGRAVVLIGRDAEKIAVAINECGVPLHRAQTMEEAVRKSFQ
ECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHH
LAREGDAVLMSPACASFDMFDNYVHRAEAFVAAVRSMQAARAGGNSAGVAGRH
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNLQGRTVLVLGLGETGLSMAKWLFRRGALVRAADTRNEPPAMRAFNSLLPQAEVFTGSL
CCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCHHHHHCCC
AGRAFYGVDLIAISPGLPLSEPLVQQALKEGIPVVGDMELFACAVGGTGGGMPKLVGITG
CCCEEECEEEEEECCCCCCCHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCCEEEEEEC
SNGKTTVTAMTGAMLKKAGWDVEVAGNIGPAVLDALMRREDTGKMPQAWVLELSSFQLET
CCCCEEEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCHHHHEEHHCCEEEH
TKSLGLDAAAVLNVSEDHLDRYAGMQEYAAAKARIFLGDSEGVQILNGDDPVVRQMTLAG
HHHCCCCCCEEECCCHHHHHHHCCHHHHHHCEEEEEEECCCCEEEECCCCHHHHHHHHCC
RPHVTFSLATPQSADDFGLLREGGDTWLMQGDTRLMDARELAITGRHNCANALAALALCR
CCCEEEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCHHEEEECCCHHHHHHHHHHHHH
ALAVPFEPLLQALREFRGLPHRVEKVAAFSGITFYDDSKGTNVGATVAALKGLGQPVVLI
HHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCCEEEE
AGGDGKAQNFSPLAAPIGEHGRAVVLIGRDAEKIAVAINECGVPLHRAQTMEEAVRKSFQ
ECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHH
LAREGDAVLMSPACASFDMFDNYVHRAEAFVAAVRSMQAARAGGNSAGVAGRH
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA