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The map label for this gene is ttcA

Identifier: 82701643

GI number: 82701643

Start: 575412

End: 576308

Strand: Direct

Name: ttcA

Synonym: Nmul_A0509

Alternate gene names: 82701643

Gene position: 575412-576308 (Clockwise)

Preceding gene: 82701636

Following gene: 82701644

Centisome position: 18.07

GC content: 54.07

Gene sequence:

>897_bases
ATGGGAACAGGAATAGAAATGTTGTCCAGAAAGGAGCAACATAACGCCAACAAACTGCATAAGCGGTTGAGGCGGCTTGT
CGGGACGGCGATCGCTGATTTCAACATGATCGAGTCTGGAGACCGGGTAATGGTGTGTCTCTCGGGCGGAAAGGACAGCT
ATGCTCTGCTCGATATCCTCCGCAGCCTGCAGGCACACGCAGCCACACAATTCGAGCTGATTGCGGTAAATCTGGACCAG
AAGCAGCCGGGCTTTCCGGAACACGTGTTGCCGAACTATCTTACATCCATCGGCATGCCTTTCCGCATCGTCGAGCAGGA
TACCTATAGCGTGGTGAAGCGCTTGATACCGGAGGGGAAAACCACTTGCAGCCTTTGTTCACGCTTGCGCCGCGGGGTCC
TGTATCGCGTGGCAGACGAACTGGGAGCAACGAAAATCGCGCTGGGCCACCATCGCGATGATATTCTTGAAACCCTGCTT
CTCAATCTGTTTCATGGCGGTAAGCTGAAAACCATGCCGCCCAAGCTGGTAAGCGATGATGGCAAGCATATCGTCATCCG
GCCGCTTGCCTACTGCAAAGAGAAAGACCTGGCCGCCTATGCGGAAATGGAAAATTTCCCGATCATCCCCTGCAACCTGT
GTGGATCACAGAAGAATATGCAGCGCCAGGTGGTAAAGGAAATGCTGCAGCAATGGGATAAAAAGTTTCCCGGCAGGCTG
GAAAACATGTTCAGCTCTTTGCAGAACATCCAACCCTCGCATCTGCTCGATTCCTCGCTTTACGATTTTCATGGGCTCAA
AACCGGGAACGGGCCTGTCGTCGACGGCGACAGGGCATTCGACCCCGAGCCCTTCGAGCCGGGCGTGGAAGAACTTCTGA
GCCTGAACAAAGACTGA

Upstream 100 bases:

>100_bases
AATCTGGGAAAATTACTGGTGCAGTTCAGAAGCCGGGATTGAACCCGAGCCTATGGGGGCGGGAACCGATGAGTATCTTG
AGAAAGAGGTTGCAGCGGGA

Downstream 100 bases:

>100_bases
AAGAATCACATCACCCTCGGCCAACTGGTGGATAAATCAAGTAAAGCACGCCTCAAATGAGAAAACCGTCCGGATCAGTT
TCGCAGCCAGCTCCACTGTC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MGTGIEMLSRKEQHNANKLHKRLRRLVGTAIADFNMIESGDRVMVCLSGGKDSYALLDILRSLQAHAATQFELIAVNLDQ
KQPGFPEHVLPNYLTSIGMPFRIVEQDTYSVVKRLIPEGKTTCSLCSRLRRGVLYRVADELGATKIALGHHRDDILETLL
LNLFHGGKLKTMPPKLVSDDGKHIVIRPLAYCKEKDLAAYAEMENFPIIPCNLCGSQKNMQRQVVKEMLQQWDKKFPGRL
ENMFSSLQNIQPSHLLDSSLYDFHGLKTGNGPVVDGDRAFDPEPFEPGVEELLSLNKD

Sequences:

>Translated_298_residues
MGTGIEMLSRKEQHNANKLHKRLRRLVGTAIADFNMIESGDRVMVCLSGGKDSYALLDILRSLQAHAATQFELIAVNLDQ
KQPGFPEHVLPNYLTSIGMPFRIVEQDTYSVVKRLIPEGKTTCSLCSRLRRGVLYRVADELGATKIALGHHRDDILETLL
LNLFHGGKLKTMPPKLVSDDGKHIVIRPLAYCKEKDLAAYAEMENFPIIPCNLCGSQKNMQRQVVKEMLQQWDKKFPGRL
ENMFSSLQNIQPSHLLDSSLYDFHGLKTGNGPVVDGDRAFDPEPFEPGVEELLSLNKD
>Mature_297_residues
GTGIEMLSRKEQHNANKLHKRLRRLVGTAIADFNMIESGDRVMVCLSGGKDSYALLDILRSLQAHAATQFELIAVNLDQK
QPGFPEHVLPNYLTSIGMPFRIVEQDTYSVVKRLIPEGKTTCSLCSRLRRGVLYRVADELGATKIALGHHRDDILETLLL
NLFHGGKLKTMPPKLVSDDGKHIVIRPLAYCKEKDLAAYAEMENFPIIPCNLCGSQKNMQRQVVKEMLQQWDKKFPGRLE
NMFSSLQNIQPSHLLDSSLYDFHGLKTGNGPVVDGDRAFDPEPFEPGVEELLSLNKD

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=285, Percent_Identity=68.0701754385965, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI193211090, Length=240, Percent_Identity=35, Blast_Score=134, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17540090, Length=235, Percent_Identity=26.3829787234043, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24651972, Length=232, Percent_Identity=26.7241379310345, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_NITMU (Q2YBQ4)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411209.1
- ProteinModelPortal:   Q2YBQ4
- SMR:   Q2YBQ4
- STRING:   Q2YBQ4
- GeneID:   3785638
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0509
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   Q2YBQ4
- ProtClustDB:   PRK10696
- BioCyc:   NMUL323848:NMUL_A0509-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 33481; Mature: 33350

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTGIEMLSRKEQHNANKLHKRLRRLVGTAIADFNMIESGDRVMVCLSGGKDSYALLDIL
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
RSLQAHAATQFELIAVNLDQKQPGFPEHVLPNYLTSIGMPFRIVEQDTYSVVKRLIPEGK
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCC
TTCSLCSRLRRGVLYRVADELGATKIALGHHRDDILETLLLNLFHGGKLKTMPPKLVSDD
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GKHIVIRPLAYCKEKDLAAYAEMENFPIIPCNLCGSQKNMQRQVVKEMLQQWDKKFPGRL
CCEEEECCHHHHCHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCHHH
ENMFSSLQNIQPSHLLDSSLYDFHGLKTGNGPVVDGDRAFDPEPFEPGVEELLSLNKD
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
GTGIEMLSRKEQHNANKLHKRLRRLVGTAIADFNMIESGDRVMVCLSGGKDSYALLDIL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHH
RSLQAHAATQFELIAVNLDQKQPGFPEHVLPNYLTSIGMPFRIVEQDTYSVVKRLIPEGK
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCC
TTCSLCSRLRRGVLYRVADELGATKIALGHHRDDILETLLLNLFHGGKLKTMPPKLVSDD
HHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
GKHIVIRPLAYCKEKDLAAYAEMENFPIIPCNLCGSQKNMQRQVVKEMLQQWDKKFPGRL
CCEEEECCHHHHCHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHCCHHH
ENMFSSLQNIQPSHLLDSSLYDFHGLKTGNGPVVDGDRAFDPEPFEPGVEELLSLNKD
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA