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The map label for this gene is tyrS

Identifier: 82701636

GI number: 82701636

Start: 560938

End: 562149

Strand: Direct

Name: tyrS

Synonym: Nmul_A0502

Alternate gene names: 82701636

Gene position: 560938-562149 (Clockwise)

Preceding gene: 82701635

Following gene: 82701643

Centisome position: 17.62

GC content: 52.39

Gene sequence:

>1212_bases
GTGACGATACCAATTTCAGAACAAATCGAAATCATCAAGCGTGGTTGCAGCGAGCTTTTGCTGGAAGACGAGCTGGAGCA
GAAGCTTGCCCAGAATCGTCCACTCAGAATCAAGGCTGGATTCGATCCTACAGCACCTGACTTGCATCTGGGTCATACCG
TTCTGCTGAATAAAATGCGCCACTTACAGGATTTAGGGCATCACGCCCTATTCCTGATTGGCGACTTCACAGGCATGATC
GGCGATCCCAGCGGCAAGAATACTACGCGTCCCCCACTTTCCCGCGAGCAGGTAGCCGAGAATGCGAAATCCTATCAGGA
CCAGGTATTCCGCATACTCAAACCTGAACAGACCGAGGTGGTGTTCAACTCCGCATGGATGGACAGGCTGAATGCCGCCG
ATCTGATCAAACTGGCGGCAACTCATACGGTTGCGCGCATGCTGGAACGGGACGATTTTGGCAAGCGCTACCAGAGCAAC
AAGCCCATTGCCATTCATGAATTTTTATATCCGTTGATCCAAGGCTATGACTCGGTCGCGCTCCGCGCAGATATCGAACT
GGGAGGCACTGACCAGAAATTCAATTTGCTGATGGGACGGGAGTTGCAGAAACACTTCGGGCAAGCGTCGCAATGCGTCA
TCACGATGCCGCTTCTCGAAGGACTGGACGGCGTCAATAAAATGTCCAAGTCTGCCGGCAACTACATCGGGATCACCGAG
AGTCCGGGGAACATGTTCGGCAAATTGATGTCTATTTCCGACGAATTGATGTGGCGGTATATTGAATTGTTGTCGTTCGA
GCCGCTGCGCGTTGTTCGTCAGTGGCAGCAGGATGTAGGGAATGGACGCAATCCTCGCGATATCAAGGTCCTGTTTGCGC
AGGAGATCGTTACGCGTTTTCATAGCAGGCAGGATGCGGAAGCCGCTCTCGCCGATTTCGAGTCACGCTTCAGGCGGGGA
GGTATCCCGGATGATTTGCCGGAAAACATACTGCAGGCTGGAAAAGAAGGATTGCCGATCGCGCAGTTGCTCAAACAGAC
CGGGCTCACCGCCAGCACCACCGAAGCCTTGCGCATGATCGAGCAAGGTGGTGTAAAATTGAATGGCGAAAAGGTGAGCG
ACAAAACTTTCAGACTGTCCTGTGGTCAAATGGTGATAGTGCAGGTGGGCAAGCGGAAGTTTGCCAGAGTAACAATCTGC
GAAGAAAAATAA

Upstream 100 bases:

>100_bases
ACCAGAACCGGCGCCCGGATTATGTCAATGCCGTGCTCGACAAGCTGATCAACTGGGGCTTTGCCGCTGACAACGATAAA
TAATACCGCGACCTGCTGAT

Downstream 100 bases:

>100_bases
AAATTATCAAAAAACCCTTGACCGGGTATCAGGCGTCTGTATAATGCCGCCTTTCGCATGATCGAGCAAGGTGATGTAAA
TCGGATGGCGAGAAAGTAAG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTIPISEQIEIIKRGCSELLLEDELEQKLAQNRPLRIKAGFDPTAPDLHLGHTVLLNKMRHLQDLGHHALFLIGDFTGMI
GDPSGKNTTRPPLSREQVAENAKSYQDQVFRILKPEQTEVVFNSAWMDRLNAADLIKLAATHTVARMLERDDFGKRYQSN
KPIAIHEFLYPLIQGYDSVALRADIELGGTDQKFNLLMGRELQKHFGQASQCVITMPLLEGLDGVNKMSKSAGNYIGITE
SPGNMFGKLMSISDELMWRYIELLSFEPLRVVRQWQQDVGNGRNPRDIKVLFAQEIVTRFHSRQDAEAALADFESRFRRG
GIPDDLPENILQAGKEGLPIAQLLKQTGLTASTTEALRMIEQGGVKLNGEKVSDKTFRLSCGQMVIVQVGKRKFARVTIC
EEK

Sequences:

>Translated_403_residues
MTIPISEQIEIIKRGCSELLLEDELEQKLAQNRPLRIKAGFDPTAPDLHLGHTVLLNKMRHLQDLGHHALFLIGDFTGMI
GDPSGKNTTRPPLSREQVAENAKSYQDQVFRILKPEQTEVVFNSAWMDRLNAADLIKLAATHTVARMLERDDFGKRYQSN
KPIAIHEFLYPLIQGYDSVALRADIELGGTDQKFNLLMGRELQKHFGQASQCVITMPLLEGLDGVNKMSKSAGNYIGITE
SPGNMFGKLMSISDELMWRYIELLSFEPLRVVRQWQQDVGNGRNPRDIKVLFAQEIVTRFHSRQDAEAALADFESRFRRG
GIPDDLPENILQAGKEGLPIAQLLKQTGLTASTTEALRMIEQGGVKLNGEKVSDKTFRLSCGQMVIVQVGKRKFARVTIC
EEK
>Mature_402_residues
TIPISEQIEIIKRGCSELLLEDELEQKLAQNRPLRIKAGFDPTAPDLHLGHTVLLNKMRHLQDLGHHALFLIGDFTGMIG
DPSGKNTTRPPLSREQVAENAKSYQDQVFRILKPEQTEVVFNSAWMDRLNAADLIKLAATHTVARMLERDDFGKRYQSNK
PIAIHEFLYPLIQGYDSVALRADIELGGTDQKFNLLMGRELQKHFGQASQCVITMPLLEGLDGVNKMSKSAGNYIGITES
PGNMFGKLMSISDELMWRYIELLSFEPLRVVRQWQQDVGNGRNPRDIKVLFAQEIVTRFHSRQDAEAALADFESRFRRGG
IPDDLPENILQAGKEGLPIAQLLKQTGLTASTTEALRMIEQGGVKLNGEKVSDKTFRLSCGQMVIVQVGKRKFARVTICE
EK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=411, Percent_Identity=27.2506082725061, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1787925, Length=405, Percent_Identity=26.9135802469136, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=401, Percent_Identity=25.6857855361596, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=415, Percent_Identity=26.2650602409639, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI20130383, Length=410, Percent_Identity=26.3414634146341, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_NITMU (Q2YBR1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411202.1
- ProteinModelPortal:   Q2YBR1
- SMR:   Q2YBR1
- STRING:   Q2YBR1
- GeneID:   3784865
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0502
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   NMUL323848:NMUL_A0502-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45312; Mature: 45181

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4; PS00435 PEROXIDASE_1

Important sites: BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPISEQIEIIKRGCSELLLEDELEQKLAQNRPLRIKAGFDPTAPDLHLGHTVLLNKMR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
HLQDLGHHALFLIGDFTGMIGDPSGKNTTRPPLSREQVAENAKSYQDQVFRILKPEQTEV
HHHHCCCCEEEEEECHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
VFNSAWMDRLNAADLIKLAATHTVARMLERDDFGKRYQSNKPIAIHEFLYPLIQGYDSVA
EEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEE
LRADIELGGTDQKFNLLMGRELQKHFGQASQCVITMPLLEGLDGVNKMSKSAGNYIGITE
EEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHCCCCEEEEEC
SPGNMFGKLMSISDELMWRYIELLSFEPLRVVRQWQQDVGNGRNPRDIKVLFAQEIVTRF
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
HSRQDAEAALADFESRFRRGGIPDDLPENILQAGKEGLPIAQLLKQTGLTASTTEALRMI
HCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHH
EQGGVKLNGEKVSDKTFRLSCGQMVIVQVGKRKFARVTICEEK
HHCCCEECCCCCCCCEEEEECCCEEEHHHCCCCEEEEEEECCC
>Mature Secondary Structure 
TIPISEQIEIIKRGCSELLLEDELEQKLAQNRPLRIKAGFDPTAPDLHLGHTVLLNKMR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
HLQDLGHHALFLIGDFTGMIGDPSGKNTTRPPLSREQVAENAKSYQDQVFRILKPEQTEV
HHHHCCCCEEEEEECHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
VFNSAWMDRLNAADLIKLAATHTVARMLERDDFGKRYQSNKPIAIHEFLYPLIQGYDSVA
EEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCEE
LRADIELGGTDQKFNLLMGRELQKHFGQASQCVITMPLLEGLDGVNKMSKSAGNYIGITE
EEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHCCCCEEEEEC
SPGNMFGKLMSISDELMWRYIELLSFEPLRVVRQWQQDVGNGRNPRDIKVLFAQEIVTRF
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
HSRQDAEAALADFESRFRRGGIPDDLPENILQAGKEGLPIAQLLKQTGLTASTTEALRMI
HCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHH
EQGGVKLNGEKVSDKTFRLSCGQMVIVQVGKRKFARVTICEEK
HHCCCEECCCCCCCCEEEEECCCEEEHHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA