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The map label for this gene is fmt

Identifier: 82701527

GI number: 82701527

Start: 434637

End: 435575

Strand: Reverse

Name: fmt

Synonym: Nmul_A0393

Alternate gene names: 82701527

Gene position: 435575-434637 (Counterclockwise)

Preceding gene: 82701528

Following gene: 82701526

Centisome position: 13.68

GC content: 52.29

Gene sequence:

>939_bases
ATGAGAATCATTTTTGCCGGAACGCCTCCTTTCGCCGCCGCCGCGCTTGAAGCCCTGGCAGATGCTGGCCACGAAATTGC
ATTGGTGCTGACCCAGCCGGATCGACCTGCCGGAAGAGGCATGAAAAATGCTTCCAGCGCAGTAAAGTTACTTGCACAAA
AGCGGGGATTTGGTCTACTGCAGCCCCCCTCCCTCAGGCAACCCGAGTTACACATGCAACTCGAGGCCGTCAGGGCAGAT
ATCATGGTTGTCGCTGCGTATGGATTGATTCTGCCTTTCTCTGTTCTGAATATCCCAAAACTCGGCTGCGTGAACATTCA
TGCCTCATTACTGCCCCGGTGGCGCGGAGCGGCTCCAATCGAACGGGCGATTCTAGCGGGAGATCGGGAAACAGGAATTA
CGATCATGCAAATGGATCGCGGTCTCGATACCGGCCCCATTCTGCTGGTACGAAGTATTACAATCGCAAAGGATGACACT
GCCGGAACATTGCATGAAAAGCTTGGACAATTGGGAGCAGCCTGTATCGTAGAAGCGCTGGCTTTGTTGCAGCAAGGAAA
AATTATCGCCACTCCCCAGAATGATCTGGCGGCCACGTACGCCTCCAAACTGGAAAAGACGGAAGCCGAAATTGACTGGC
GGATGGATGCAGAAAATATCGATCGCGCAGTGAGGGCCTTTAATCCTCGTCCCGGCATGCATTCCACCGTGAATGGAATT
TCCATGAAAGTGTGGCAGACCAGCGTAGATATCGCAGAGACAGATGAAAAACCGGGGGAAATCGTTGCAATAAGACCGGA
CGGAATTGTCGTTGCATGCGGCAAGCATGCCTTGATATTGAAGATCGTGCAAAAATCCGGAGGTAAAAAATTAAGATCCG
CTGAATTCCTCCTGGGGCATTCTCTGCACCCGCACGATCGTTTTGAAAGCGGGGAATGA

Upstream 100 bases:

>100_bases
GGGAGATTATCCGGAGAATTTGGTGACCGGTGCTCCGGGAACGGGATGCGGATACTGACCATCCTTTCTTGCCGTCACGC
GCCTACCCCTGCCCATCCCC

Downstream 100 bases:

>100_bases
TCAGGACGCAGCGTCTCGCCGCCACCACTGTTGAAAGAGTGGTGAGTGGCGCAAGTCTGACAACGGTTTTGCAGGAAACC
TGGCGCAGCCACAGCGGCCT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRIIFAGTPPFAAAALEALADAGHEIALVLTQPDRPAGRGMKNASSAVKLLAQKRGFGLLQPPSLRQPELHMQLEAVRAD
IMVVAAYGLILPFSVLNIPKLGCVNIHASLLPRWRGAAPIERAILAGDRETGITIMQMDRGLDTGPILLVRSITIAKDDT
AGTLHEKLGQLGAACIVEALALLQQGKIIATPQNDLAATYASKLEKTEAEIDWRMDAENIDRAVRAFNPRPGMHSTVNGI
SMKVWQTSVDIAETDEKPGEIVAIRPDGIVVACGKHALILKIVQKSGGKKLRSAEFLLGHSLHPHDRFESGE

Sequences:

>Translated_312_residues
MRIIFAGTPPFAAAALEALADAGHEIALVLTQPDRPAGRGMKNASSAVKLLAQKRGFGLLQPPSLRQPELHMQLEAVRAD
IMVVAAYGLILPFSVLNIPKLGCVNIHASLLPRWRGAAPIERAILAGDRETGITIMQMDRGLDTGPILLVRSITIAKDDT
AGTLHEKLGQLGAACIVEALALLQQGKIIATPQNDLAATYASKLEKTEAEIDWRMDAENIDRAVRAFNPRPGMHSTVNGI
SMKVWQTSVDIAETDEKPGEIVAIRPDGIVVACGKHALILKIVQKSGGKKLRSAEFLLGHSLHPHDRFESGE
>Mature_312_residues
MRIIFAGTPPFAAAALEALADAGHEIALVLTQPDRPAGRGMKNASSAVKLLAQKRGFGLLQPPSLRQPELHMQLEAVRAD
IMVVAAYGLILPFSVLNIPKLGCVNIHASLLPRWRGAAPIERAILAGDRETGITIMQMDRGLDTGPILLVRSITIAKDDT
AGTLHEKLGQLGAACIVEALALLQQGKIIATPQNDLAATYASKLEKTEAEIDWRMDAENIDRAVRAFNPRPGMHSTVNGI
SMKVWQTSVDIAETDEKPGEIVAIRPDGIVVACGKHALILKIVQKSGGKKLRSAEFLLGHSLHPHDRFESGE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=321, Percent_Identity=28.9719626168224, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI21614513, Length=316, Percent_Identity=27.2151898734177, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=32.7659574468085, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789683, Length=297, Percent_Identity=49.1582491582492, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788589, Length=297, Percent_Identity=27.9461279461279, Blast_Score=134, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI133930964, Length=265, Percent_Identity=28.6792452830189, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550868, Length=301, Percent_Identity=29.5681063122924, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24585660, Length=240, Percent_Identity=31.25, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571984, Length=262, Percent_Identity=30.1526717557252, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_NITMU (Q2YC20)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411093.1
- ProteinModelPortal:   Q2YC20
- SMR:   Q2YC20
- STRING:   Q2YC20
- GeneID:   3785386
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0393
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q2YC20
- ProtClustDB:   CLSK586047
- BioCyc:   NMUL323848:NMUL_A0393-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33547; Mature: 33547

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIFAGTPPFAAAALEALADAGHEIALVLTQPDRPAGRGMKNASSAVKLLAQKRGFGLL
CEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
QPPSLRQPELHMQLEAVRADIMVVAAYGLILPFSVLNIPKLGCVNIHASLLPRWRGAAPI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCH
ERAILAGDRETGITIMQMDRGLDTGPILLVRSITIAKDDTAGTLHEKLGQLGAACIVEAL
HHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ALLQQGKIIATPQNDLAATYASKLEKTEAEIDWRMDAENIDRAVRAFNPRPGMHSTVNGI
HHHHCCCEEECCCHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCCCCCCCCCC
SMKVWQTSVDIAETDEKPGEIVAIRPDGIVVACGKHALILKIVQKSGGKKLRSAEFLLGH
EEEEEEEECCCCCCCCCCCCEEEECCCCEEEECCCCEEEHHHHHHCCCCCHHHHHHHHCC
SLHPHDRFESGE
CCCCCCCCCCCC
>Mature Secondary Structure
MRIIFAGTPPFAAAALEALADAGHEIALVLTQPDRPAGRGMKNASSAVKLLAQKRGFGLL
CEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCC
QPPSLRQPELHMQLEAVRADIMVVAAYGLILPFSVLNIPKLGCVNIHASLLPRWRGAAPI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCCCCCH
ERAILAGDRETGITIMQMDRGLDTGPILLVRSITIAKDDTAGTLHEKLGQLGAACIVEAL
HHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ALLQQGKIIATPQNDLAATYASKLEKTEAEIDWRMDAENIDRAVRAFNPRPGMHSTVNGI
HHHHCCCEEECCCHHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHCCCCCCCCCCCCCC
SMKVWQTSVDIAETDEKPGEIVAIRPDGIVVACGKHALILKIVQKSGGKKLRSAEFLLGH
EEEEEEEECCCCCCCCCCCCEEEECCCCEEEECCCCEEEHHHHHHCCCCCHHHHHHHHCC
SLHPHDRFESGE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA