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The map label for this gene is def2 [H]

Identifier: 82701528

GI number: 82701528

Start: 435780

End: 436355

Strand: Reverse

Name: def2 [H]

Synonym: Nmul_A0394

Alternate gene names: 82701528

Gene position: 436355-435780 (Counterclockwise)

Preceding gene: 82701531

Following gene: 82701527

Centisome position: 13.7

GC content: 53.3

Gene sequence:

>576_bases
GTGCGACGGACTATCGCTATCAGGCGTCGCCTTGAATTTTGCATTCAGTTTCTTTTATGTTCAAGCCTTTTTATGGCGCT
ATTGAAAATATTGCAATATCCTGACGAACGGTTACACACCATTGCGGCTCCGGTTCCCGAAGTCACCGATGAAATACGCG
TCCTGGTGCAGGATATGGCGGAAACCATGTATGCCGCGCCGGGAGTCGGGTTGGCCGCGACGCAGGTGGACGTGCACAAG
CGTGTTATCGTAATCGATGTTTCCAATACCCATGATCAGCTGCTTGTGCTGATTAATCCGGAAATCACCACGCACGAGGG
GGAATCGGACTATGAGGAAGGCTGCCTGTCCGTACCTGGCATATTTGGCAAGGTGCCGCGCGCTGCGCAGATTACCGTCG
AAGCCCTGAACAAGGACGGCGAGCGCTTCACGCTGGATGCGGACGGGCTTCTGGCGGTGTGTATCCAGCATGAAATGGAT
CACCTGCTGGGAAGAGTATTTGTGGAGTATTGGTCGCGGATGAAGCAATCGAGGATTCAGGCAAAATTCCGGAAGCAGCG
CCGAAAGGTAATGTAG

Upstream 100 bases:

>100_bases
CAATCTGATGAAATATAGGAACTTAGCAGTGATTGAAAGTCGGAGAAAAGAAGGTTGCAGGTAAATCAGGATATAATATA
TCCGCAGGAATCCTGCGAAG

Downstream 100 bases:

>100_bases
CAGACCATGTAAAAATTTATATCTTTATTATCCGTCTCTTGCACGCTCTTGGTCCATTCCACGGCGCATTCCCTGTTTCC
ACAGCAAACCGATGCAACCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MRRTIAIRRRLEFCIQFLLCSSLFMALLKILQYPDERLHTIAAPVPEVTDEIRVLVQDMAETMYAAPGVGLAATQVDVHK
RVIVIDVSNTHDQLLVLINPEITTHEGESDYEEGCLSVPGIFGKVPRAAQITVEALNKDGERFTLDADGLLAVCIQHEMD
HLLGRVFVEYWSRMKQSRIQAKFRKQRRKVM

Sequences:

>Translated_191_residues
MRRTIAIRRRLEFCIQFLLCSSLFMALLKILQYPDERLHTIAAPVPEVTDEIRVLVQDMAETMYAAPGVGLAATQVDVHK
RVIVIDVSNTHDQLLVLINPEITTHEGESDYEEGCLSVPGIFGKVPRAAQITVEALNKDGERFTLDADGLLAVCIQHEMD
HLLGRVFVEYWSRMKQSRIQAKFRKQRRKVM
>Mature_191_residues
MRRTIAIRRRLEFCIQFLLCSSLFMALLKILQYPDERLHTIAAPVPEVTDEIRVLVQDMAETMYAAPGVGLAATQVDVHK
RVIVIDVSNTHDQLLVLINPEITTHEGESDYEEGCLSVPGIFGKVPRAAQITVEALNKDGERFTLDADGLLAVCIQHEMD
HLLGRVFVEYWSRMKQSRIQAKFRKQRRKVM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=29.375, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=56.7073170731707, Blast_Score=197, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24645726, Length=162, Percent_Identity=29.0123456790123, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21775; Mature: 21775

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTIAIRRRLEFCIQFLLCSSLFMALLKILQYPDERLHTIAAPVPEVTDEIRVLVQDMA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHH
ETMYAAPGVGLAATQVDVHKRVIVIDVSNTHDQLLVLINPEITTHEGESDYEEGCLSVPG
HHHHCCCCCCHHHHEEHHHCEEEEEEECCCCCEEEEEECCCEECCCCCCCHHHCCCCCCC
IFGKVPRAAQITVEALNKDGERFTLDADGLLAVCIQHEMDHLLGRVFVEYWSRMKQSRIQ
HHCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKFRKQRRKVM
HHHHHHHHCCC
>Mature Secondary Structure
MRRTIAIRRRLEFCIQFLLCSSLFMALLKILQYPDERLHTIAAPVPEVTDEIRVLVQDMA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHH
ETMYAAPGVGLAATQVDVHKRVIVIDVSNTHDQLLVLINPEITTHEGESDYEEGCLSVPG
HHHHCCCCCCHHHHEEHHHCEEEEEEECCCCCEEEEEECCCEECCCCCCCHHHCCCCCCC
IFGKVPRAAQITVEALNKDGERFTLDADGLLAVCIQHEMDHLLGRVFVEYWSRMKQSRIQ
HHCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKFRKQRRKVM
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]