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The map label for this gene is rsmB [H]

Identifier: 82701526

GI number: 82701526

Start: 433312

End: 434640

Strand: Reverse

Name: rsmB [H]

Synonym: Nmul_A0392

Alternate gene names: 82701526

Gene position: 434640-433312 (Counterclockwise)

Preceding gene: 82701527

Following gene: 82701525

Centisome position: 13.65

GC content: 55.68

Gene sequence:

>1329_bases
ATGATCAGGACGCAGCGTCTCGCCGCCACCACTGTTGAAAGAGTGGTGAGTGGCGCAAGTCTGACAACGGTTTTGCAGGA
AACCTGGCGCAGCCACAGCGGCCTCTCCGACCAGCAGCGCGGTGCGATTCAGGATTTAAGCTATGGCGTACTGCGCTTTT
ATGGTCAACTGGACATATTGCTTGGCTTATTGCTGAACAAACCCCTGAAGGACCAGAGTTTGCGCTACCTGCTACTGGTG
GGACTGTATCAGCTTCAATACAGCAAAACGGCGCCGCACGTCGTGGTGGACAGTGCAGTATCCGCTTCTTGCAGCCCCTC
GATCGATGGCAGGAATAGCAGGAACATGCTGGATCCCCGCCATGCGAAAAGTATAGGCGGACTGGTCAATGCGATATTGC
GCAACTTCTTGCGCAAACGTCCCGCCCTGCTTGAAAAAACGGCGGCAACCGAAGTAGGAAGGTATTCCCATCCACAATGG
TGGATCGACAAACTCCGTGCGCAATATCCCTCCCGCTATCAGGCTGTACTGGAGACAGCCAATATACGACCACCTATGGC
GCTCAGAGTCAATCAGCGGAGGACAAACGTTGAAGCATATCAGAAGCTGCTGCACAATGCCGGACTGAATGCCCGGCGAC
CGGAAAACGAGTGGACAGAAGAGGCGCTTGAATTATTTCACCCGGTCCCGGTGGAAAAACTGCCGGGCTTCGGTCAAGGA
CTGGTATCAGTTCAGGATGTGGCCGCTCAAATGGCAGCGCCGCTACTCTGTCCCCAGAGCGGAATGCGGGTGCTGGACGC
CTGCGCTGCCCCGGGCGGGAAAAGCGCGCATCTGCTTGAATTGACCGATCTGGAACTGACCGCGGTTGACAATGATGGCG
AGCGGTTAGAACGGCTAAAGCAGAATTTCACTCGTCTCGGCCTCAAGCCTTATCGCATCATTCACGGCGACGCCACGCAC
CCGGGGGAGTGGTGGGATGGAAGGCCATTCGATCGTATCCTGGCTGATGCCCCCTGCTCTGCGTCCGGGGTGGTGCGCCG
TCACCCCGACATCAAATGGCTGCGACGCGAAAGCGACCTGGCGCAGTTTGCCGAGATACAACGTAAAACCCTCGATGCCC
TTTGGCAAACCCTGGTCAAAGGCGGTAAATTGCTCTATGTCACTTGTTCCATATTCGCAGAGGAAAACGGTCTTCAGGTG
GAAAGCTTCCTGAATCATCATCCGGATGCTCGTCTACTGCCCTTTTCCGTGCCGGAAATCGTGGAGGGCCAGTTGTTGCC
GGACCTCCATCACGACGGTTTTTTTTATGCATTGCTTGAAAAGCTCTGA

Upstream 100 bases:

>100_bases
TATTGAAGATCGTGCAAAAATCCGGAGGTAAAAAATTAAGATCCGCTGAATTCCTCCTGGGGCATTCTCTGCACCCGCAC
GATCGTTTTGAAAGCGGGGA

Downstream 100 bases:

>100_bases
TTTCCTGCCCCGTCTCCTGCGCATGACTGTGGCAGCGCTGCTGCTTGCGCTCATAGGACAGGCATCCAGCGCACATGGGG
AAGCAAGCATAGAGATCAAG

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MIRTQRLAATTVERVVSGASLTTVLQETWRSHSGLSDQQRGAIQDLSYGVLRFYGQLDILLGLLLNKPLKDQSLRYLLLV
GLYQLQYSKTAPHVVVDSAVSASCSPSIDGRNSRNMLDPRHAKSIGGLVNAILRNFLRKRPALLEKTAATEVGRYSHPQW
WIDKLRAQYPSRYQAVLETANIRPPMALRVNQRRTNVEAYQKLLHNAGLNARRPENEWTEEALELFHPVPVEKLPGFGQG
LVSVQDVAAQMAAPLLCPQSGMRVLDACAAPGGKSAHLLELTDLELTAVDNDGERLERLKQNFTRLGLKPYRIIHGDATH
PGEWWDGRPFDRILADAPCSASGVVRRHPDIKWLRRESDLAQFAEIQRKTLDALWQTLVKGGKLLYVTCSIFAEENGLQV
ESFLNHHPDARLLPFSVPEIVEGQLLPDLHHDGFFYALLEKL

Sequences:

>Translated_442_residues
MIRTQRLAATTVERVVSGASLTTVLQETWRSHSGLSDQQRGAIQDLSYGVLRFYGQLDILLGLLLNKPLKDQSLRYLLLV
GLYQLQYSKTAPHVVVDSAVSASCSPSIDGRNSRNMLDPRHAKSIGGLVNAILRNFLRKRPALLEKTAATEVGRYSHPQW
WIDKLRAQYPSRYQAVLETANIRPPMALRVNQRRTNVEAYQKLLHNAGLNARRPENEWTEEALELFHPVPVEKLPGFGQG
LVSVQDVAAQMAAPLLCPQSGMRVLDACAAPGGKSAHLLELTDLELTAVDNDGERLERLKQNFTRLGLKPYRIIHGDATH
PGEWWDGRPFDRILADAPCSASGVVRRHPDIKWLRRESDLAQFAEIQRKTLDALWQTLVKGGKLLYVTCSIFAEENGLQV
ESFLNHHPDARLLPFSVPEIVEGQLLPDLHHDGFFYALLEKL
>Mature_442_residues
MIRTQRLAATTVERVVSGASLTTVLQETWRSHSGLSDQQRGAIQDLSYGVLRFYGQLDILLGLLLNKPLKDQSLRYLLLV
GLYQLQYSKTAPHVVVDSAVSASCSPSIDGRNSRNMLDPRHAKSIGGLVNAILRNFLRKRPALLEKTAATEVGRYSHPQW
WIDKLRAQYPSRYQAVLETANIRPPMALRVNQRRTNVEAYQKLLHNAGLNARRPENEWTEEALELFHPVPVEKLPGFGQG
LVSVQDVAAQMAAPLLCPQSGMRVLDACAAPGGKSAHLLELTDLELTAVDNDGERLERLKQNFTRLGLKPYRIIHGDATH
PGEWWDGRPFDRILADAPCSASGVVRRHPDIKWLRRESDLAQFAEIQRKTLDALWQTLVKGGKLLYVTCSIFAEENGLQV
ESFLNHHPDARLLPFSVPEIVEGQLLPDLHHDGFFYALLEKL

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=344, Percent_Identity=30.2325581395349, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI76150623, Length=344, Percent_Identity=30.2325581395349, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI32698918, Length=201, Percent_Identity=34.8258706467662, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI301336155, Length=200, Percent_Identity=29, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI39995082, Length=200, Percent_Identity=29, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI2367212, Length=440, Percent_Identity=41.1363636363636, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI87081985, Length=308, Percent_Identity=28.2467532467532, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=319, Percent_Identity=30.4075235109718, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324268, Length=251, Percent_Identity=29.4820717131474, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI22024126, Length=346, Percent_Identity=28.9017341040462, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356579, Length=219, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49598; Mature: 49598

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTQRLAATTVERVVSGASLTTVLQETWRSHSGLSDQQRGAIQDLSYGVLRFYGQLDIL
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
LGLLLNKPLKDQSLRYLLLVGLYQLQYSKTAPHVVVDSAVSASCSPSIDGRNSRNMLDPR
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCCCCCCCCCCHH
HAKSIGGLVNAILRNFLRKRPALLEKTAATEVGRYSHPQWWIDKLRAQYPSRYQAVLETA
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHC
NIRPPMALRVNQRRTNVEAYQKLLHNAGLNARRPENEWTEEALELFHPVPVEKLPGFGQG
CCCCCEEEEECCHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCH
LVSVQDVAAQMAAPLLCPQSGMRVLDACAAPGGKSAHLLELTDLELTAVDNDGERLERLK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECEEEEEECCCHHHHHHHH
QNFTRLGLKPYRIIHGDATHPGEWWDGRPFDRILADAPCSASGVVRRHPDIKWLRRESDL
HHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHH
AQFAEIQRKTLDALWQTLVKGGKLLYVTCSIFAEENGLQVESFLNHHPDARLLPFSVPEI
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHCCCCCCEECCCCCHHH
VEGQLLPDLHHDGFFYALLEKL
HCCCCCCCCCCCHHHHHHHHHC
>Mature Secondary Structure
MIRTQRLAATTVERVVSGASLTTVLQETWRSHSGLSDQQRGAIQDLSYGVLRFYGQLDIL
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
LGLLLNKPLKDQSLRYLLLVGLYQLQYSKTAPHVVVDSAVSASCSPSIDGRNSRNMLDPR
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCCCCCCCCCCCCCCCHH
HAKSIGGLVNAILRNFLRKRPALLEKTAATEVGRYSHPQWWIDKLRAQYPSRYQAVLETA
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHC
NIRPPMALRVNQRRTNVEAYQKLLHNAGLNARRPENEWTEEALELFHPVPVEKLPGFGQG
CCCCCEEEEECCHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCH
LVSVQDVAAQMAAPLLCPQSGMRVLDACAAPGGKSAHLLELTDLELTAVDNDGERLERLK
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECEEEEEECCCHHHHHHHH
QNFTRLGLKPYRIIHGDATHPGEWWDGRPFDRILADAPCSASGVVRRHPDIKWLRRESDL
HHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHH
AQFAEIQRKTLDALWQTLVKGGKLLYVTCSIFAEENGLQVESFLNHHPDARLLPFSVPEI
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHCCCCCCEECCCCCHHH
VEGQLLPDLHHDGFFYALLEKL
HCCCCCCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]