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The map label for this gene is ntrX [H]

Identifier: 82701523

GI number: 82701523

Start: 429178

End: 430476

Strand: Reverse

Name: ntrX [H]

Synonym: Nmul_A0389

Alternate gene names: 82701523

Gene position: 430476-429178 (Counterclockwise)

Preceding gene: 82701524

Following gene: 82701522

Centisome position: 13.52

GC content: 56.51

Gene sequence:

>1299_bases
TTGACGTTCAATTTCATTTCCGTGGCAAGCAAATCTATTCCTGGAATTATGAGCAACAATACGATACTGATCGTTGACGA
TGAAATCGGCATCCGTGAGCTTTTGTCCGAAATCCTCCGGGATGAGGGTTATCGCGTCGCCCTGGCAGAAAATGCCGCAC
AGGCGCGCGCATGGCGGAAACAGGCTCGCCCCGATCTGGTATTGCTGGATATCTGGATGCCGGATACCGACGGCATCACT
CTGCTGAAAGAATGGGTCAGCAGCGGACTTCTCACCATGCCGGTGGTGATGATGTCGGGCCACGGCACAATCGACACAGC
GGTAGAAGCCACGCGCATGGGGGCAGCGGCCTATCTCGAGAAACCGGTACCGCTGCAAAAACTGCTGAGCACAGTCGGCC
GGGCGTTGCGCAGCGGGCAGGCCAAGCCGCGCATGACACTTCCCCTGGCAAGCCTGGGACGAGGGGCGCTGATTACAGAC
CTGAAAAGACGGCTGGAACAGGTTGCCAGCCTCACGGCACCGGTGCTCCTCATAAGTGAACCGGGAACGGGCGTGGATCT
GTGCGCCCGCTTTCTGCACGAGCCAAATACACCTTGGGTAGAACCCGATACTCTCGCCACCCTGTCGAATGCTCCGTTTG
AGTTACTTGAACAAGCAAAAGGAGGATTGCTTTTTCTCAAGGAAATCGACGAGCTCAACAAACTGGCGCAAAAAGGACTG
TTGCTGCTGCTGGGCAAGCTGGAAAGATACAGTGTCCGGCTCGTATGCGTGACCTCCACGCCGCTTGCCAGCCTGGCAGC
TCAAGGGGTCTACGACCCCAAGCTGTATGAGATGCTGAGCGGACTTTGCATCAACGTGCCCGCACTAAGGGAGCATCGGG
AAGACATTCCCGAACTCGCGACCCAGATACTCTCGCGTTACATCGAATCAGGAGACGTTCCGTTGCGACAGTTCAGCACC
GCTGCCCTCAACTGCCTGCGAAATCACGACTGGCCCGGAAATCTCGCACAATTGACGGGCGCAGTGCATACCCTTGCGCT
GAGTTGCACCAGCGATGAAATATCGGCAGAAGACGTAAAACAGATACTGGTCCCTTCCTCGCCTTCACAGGCAGTGGCGG
GTATCCCGCTCGACATACCGTTGCGTGAAGCGCGGGACATGTTCGAAAAAACGTATTTTGAACAGCTTATCGAACAGGAA
AACGGCAATATGACCCGAGTGGCGGAACGTGCCGGCCTGGAGCGCACCCATCTGTACCGCAAGCTCAAACTGCTCGGCAT
CAGGCTGCGCGGGAATTGA

Upstream 100 bases:

>100_bases
ACTCGTGGTTTCTCTCAAAGATGGATGGTTCCGACCGATCGCGGGAGAGCCAGCGCTTTCACTTTCCTGTGAACTGCAGT
CAAAAGAATAAGAGGTAAGC

Downstream 100 bases:

>100_bases
GATTCATTCTCTACAATCGGAGTTGTCATATTGTCTGGCATCCTATCTTAAGAGATAATAATGGTTTTGCCGACCCGCAC
CGCTATTTGAGCGGTGTTTT

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTFNFISVASKSIPGIMSNNTILIVDDEIGIRELLSEILRDEGYRVALAENAAQARAWRKQARPDLVLLDIWMPDTDGIT
LLKEWVSSGLLTMPVVMMSGHGTIDTAVEATRMGAAAYLEKPVPLQKLLSTVGRALRSGQAKPRMTLPLASLGRGALITD
LKRRLEQVASLTAPVLLISEPGTGVDLCARFLHEPNTPWVEPDTLATLSNAPFELLEQAKGGLLFLKEIDELNKLAQKGL
LLLLGKLERYSVRLVCVTSTPLASLAAQGVYDPKLYEMLSGLCINVPALREHREDIPELATQILSRYIESGDVPLRQFST
AALNCLRNHDWPGNLAQLTGAVHTLALSCTSDEISAEDVKQILVPSSPSQAVAGIPLDIPLREARDMFEKTYFEQLIEQE
NGNMTRVAERAGLERTHLYRKLKLLGIRLRGN

Sequences:

>Translated_432_residues
MTFNFISVASKSIPGIMSNNTILIVDDEIGIRELLSEILRDEGYRVALAENAAQARAWRKQARPDLVLLDIWMPDTDGIT
LLKEWVSSGLLTMPVVMMSGHGTIDTAVEATRMGAAAYLEKPVPLQKLLSTVGRALRSGQAKPRMTLPLASLGRGALITD
LKRRLEQVASLTAPVLLISEPGTGVDLCARFLHEPNTPWVEPDTLATLSNAPFELLEQAKGGLLFLKEIDELNKLAQKGL
LLLLGKLERYSVRLVCVTSTPLASLAAQGVYDPKLYEMLSGLCINVPALREHREDIPELATQILSRYIESGDVPLRQFST
AALNCLRNHDWPGNLAQLTGAVHTLALSCTSDEISAEDVKQILVPSSPSQAVAGIPLDIPLREARDMFEKTYFEQLIEQE
NGNMTRVAERAGLERTHLYRKLKLLGIRLRGN
>Mature_431_residues
TFNFISVASKSIPGIMSNNTILIVDDEIGIRELLSEILRDEGYRVALAENAAQARAWRKQARPDLVLLDIWMPDTDGITL
LKEWVSSGLLTMPVVMMSGHGTIDTAVEATRMGAAAYLEKPVPLQKLLSTVGRALRSGQAKPRMTLPLASLGRGALITDL
KRRLEQVASLTAPVLLISEPGTGVDLCARFLHEPNTPWVEPDTLATLSNAPFELLEQAKGGLLFLKEIDELNKLAQKGLL
LLLGKLERYSVRLVCVTSTPLASLAAQGVYDPKLYEMLSGLCINVPALREHREDIPELATQILSRYIESGDVPLRQFSTA
ALNCLRNHDWPGNLAQLTGAVHTLALSCTSDEISAEDVKQILVPSSPSQAVAGIPLDIPLREARDMFEKTYFEQLIEQEN
GNMTRVAERAGLERTHLYRKLKLLGIRLRGN

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=449, Percent_Identity=31.4031180400891, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=29.7356828193833, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1788905, Length=437, Percent_Identity=30.2059496567506, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=26.6524520255864, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1787583, Length=295, Percent_Identity=27.1186440677966, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=28.3018867924528, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082152, Length=310, Percent_Identity=27.4193548387097, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081858, Length=291, Percent_Identity=27.8350515463918, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=24.2138364779874, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082117, Length=329, Percent_Identity=25.8358662613982, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787375, Length=129, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786838, Length=116, Percent_Identity=37.9310344827586, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=24.7619047619048, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786524, Length=320, Percent_Identity=26.5625, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=120, Percent_Identity=34.1666666666667, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786911, Length=209, Percent_Identity=29.1866028708134, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47500; Mature: 47369

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNFISVASKSIPGIMSNNTILIVDDEIGIRELLSEILRDEGYRVALAENAAQARAWRK
CCCCHHHHHHHCCCCEECCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
QARPDLVLLDIWMPDTDGITLLKEWVSSGLLTMPVVMMSGHGTIDTAVEATRMGAAAYLE
HCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
KPVPLQKLLSTVGRALRSGQAKPRMTLPLASLGRGALITDLKRRLEQVASLTAPVLLISE
CCCCHHHHHHHHHHHHHCCCCCCCEECCHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEC
PGTGVDLCARFLHEPNTPWVEPDTLATLSNAPFELLEQAKGGLLFLKEIDELNKLAQKGL
CCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHH
LLLLGKLERYSVRLVCVTSTPLASLAAQGVYDPKLYEMLSGLCINVPALREHREDIPELA
HHHHHHHCCEEEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TQILSRYIESGDVPLRQFSTAALNCLRNHDWPGNLAQLTGAVHTLALSCTSDEISAEDVK
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHHH
QILVPSSPSQAVAGIPLDIPLREARDMFEKTYFEQLIEQENGNMTRVAERAGLERTHLYR
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHH
KLKLLGIRLRGN
HHHHHEEEEECC
>Mature Secondary Structure 
TFNFISVASKSIPGIMSNNTILIVDDEIGIRELLSEILRDEGYRVALAENAAQARAWRK
CCCHHHHHHHCCCCEECCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
QARPDLVLLDIWMPDTDGITLLKEWVSSGLLTMPVVMMSGHGTIDTAVEATRMGAAAYLE
HCCCCEEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
KPVPLQKLLSTVGRALRSGQAKPRMTLPLASLGRGALITDLKRRLEQVASLTAPVLLISE
CCCCHHHHHHHHHHHHHCCCCCCCEECCHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEC
PGTGVDLCARFLHEPNTPWVEPDTLATLSNAPFELLEQAKGGLLFLKEIDELNKLAQKGL
CCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHH
LLLLGKLERYSVRLVCVTSTPLASLAAQGVYDPKLYEMLSGLCINVPALREHREDIPELA
HHHHHHHCCEEEEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
TQILSRYIESGDVPLRQFSTAALNCLRNHDWPGNLAQLTGAVHTLALSCTSDEISAEDVK
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHHH
QILVPSSPSQAVAGIPLDIPLREARDMFEKTYFEQLIEQENGNMTRVAERAGLERTHLYR
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHH
KLKLLGIRLRGN
HHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]