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The map label for this gene is gcvH

Identifier: 82701347

GI number: 82701347

Start: 226582

End: 226971

Strand: Reverse

Name: gcvH

Synonym: Nmul_A0213

Alternate gene names: 82701347

Gene position: 226971-226582 (Counterclockwise)

Preceding gene: 82701348

Following gene: 82701346

Centisome position: 7.13

GC content: 53.08

Gene sequence:

>390_bases
ATGAGTATTCCGAATGATCTGAAATACACGAAATCCCATGAATGGGTGCGTCTCGAAGATGACGGCACGGCAACTATCGG
TATTACCCAGCATGCTCAGGAGTTGCTCGGGGACATGGTATTCGTTGAAACCCCCGCAGTGGGCCGCCAGCTCAAGCAAG
GGGATGAATGTGCCGTAGTTGAATCGGTAAAGGCGGCAGCCGATGTTTACGCGCCGATTACGGGAGAAGTCACCGCCGCT
AATCCGGAACTCGCCTCCGCTCCGGAAAAAATCAACCAGGACGCGTATGCGGCCTGGCTTTTCAGACTGAAACCGGCAAA
CACCGCTGATCTGGAAAAATTGCTCGATTCTGCCGGATACCAGAAACTGCTCGAAAGCGAAGACCATTAA

Upstream 100 bases:

>100_bases
GGCTGAAAGCGAAGGTCGTGAAATATCCTTTCGTCCGCAATGGCAAGATTCTCGTTTGATGAAACGGCAAAGCACTATCA
TTTAATTCTGGAGCAAAGAC

Downstream 100 bases:

>100_bases
TCAAATCACTTGAGGGAAATGGAACGGGGCCTTGCTCCCGCTGTCATTTCCCTGCCTTTTACGCCTCACCGATGATTCAC
GGAACAAATCATGCCGTTCA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSIPNDLKYTKSHEWVRLEDDGTATIGITQHAQELLGDMVFVETPAVGRQLKQGDECAVVESVKAAADVYAPITGEVTAA
NPELASAPEKINQDAYAAWLFRLKPANTADLEKLLDSAGYQKLLESEDH

Sequences:

>Translated_129_residues
MSIPNDLKYTKSHEWVRLEDDGTATIGITQHAQELLGDMVFVETPAVGRQLKQGDECAVVESVKAAADVYAPITGEVTAA
NPELASAPEKINQDAYAAWLFRLKPANTADLEKLLDSAGYQKLLESEDH
>Mature_128_residues
SIPNDLKYTKSHEWVRLEDDGTATIGITQHAQELLGDMVFVETPAVGRQLKQGDECAVVESVKAAADVYAPITGEVTAAN
PELASAPEKINQDAYAAWLFRLKPANTADLEKLLDSAGYQKLLESEDH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=41.6666666666667, Blast_Score=104, Evalue=2e-23,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=40.8333333333333, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=55.1181102362205, Blast_Score=157, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17507493, Length=121, Percent_Identity=46.2809917355372, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=125, Percent_Identity=40, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=129, Percent_Identity=36.4341085271318, Blast_Score=94, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17865652, Length=129, Percent_Identity=41.0852713178295, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_NITMU (Q2YCK0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_410913.1
- ProteinModelPortal:   Q2YCK0
- SMR:   Q2YCK0
- STRING:   Q2YCK0
- GeneID:   3784590
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0213
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q2YCK0
- ProtClustDB:   PRK01202
- BioCyc:   NMUL323848:NMUL_A0213-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14056; Mature: 13925

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNDLKYTKSHEWVRLEDDGTATIGITQHAQELLGDMVFVETPAVGRQLKQGDECAVV
CCCCCCCCCCCCCCEEEECCCCCEEEEHHHHHHHHHHCEEEEECCHHHHHHHCCCCHHHH
ESVKAAADVYAPITGEVTAANPELASAPEKINQDAYAAWLFRLKPANTADLEKLLDSAGY
HHHHHHHHHCCCCCCCEEECCCCHHCCHHHHCHHHHHHHHEEECCCCHHHHHHHHHHHHH
QKLLESEDH
HHHHCCCCC
>Mature Secondary Structure 
SIPNDLKYTKSHEWVRLEDDGTATIGITQHAQELLGDMVFVETPAVGRQLKQGDECAVV
CCCCCCCCCCCCCEEEECCCCCEEEEHHHHHHHHHHCEEEEECCHHHHHHHCCCCHHHH
ESVKAAADVYAPITGEVTAANPELASAPEKINQDAYAAWLFRLKPANTADLEKLLDSAGY
HHHHHHHHHCCCCCCCEEECCCCHHCCHHHHCHHHHHHHHEEECCCCHHHHHHHHHHHHH
QKLLESEDH
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA