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The map label for this gene is yjfC [H]

Identifier: 82546572

GI number: 82546572

Start: 4319415

End: 4320473

Strand: Reverse

Name: yjfC [H]

Synonym: SBO_4269

Alternate gene names: 82546572

Gene position: 4320473-4319415 (Counterclockwise)

Preceding gene: 82546573

Following gene: 82546571

Centisome position: 95.59

GC content: 52.88

Gene sequence:

>1059_bases
ATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTAACAACGGTTTCGACTTTCATATCATCGA
CAATGAAATCTATTGGGACGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAACCGACTG
CGGAACTGCATCAGATGTGCCTTGAGGTGGTGGATCGCGCGGTTAAAGACGAAGAGATCCTGACGCAACTGGCGATCCCG
CCGTTGTACTGGGATGTGATCGCCGAAAGCTGGCGCGCACGCGATCCTTCGCTGTATGGCCGGATGGATTTTGCCTGGTG
TGGCAATGCGCCGGTGAAGCTGCTGGAGTACAACGCCATTCAGGAACGCCTGATTTCGCGCTTTAGTGAGCTTTACAGTC
GGGAACCGTTTTATTTTTGCTGCTGTCAGGACACCGATGAAGACAGGAGTACCGTGCTGTACTTGCAGGACTGCGCCCAG
CAGGCAGGGCAGGAGTCGCGGTTTATCTACATTGAAGATCTCGGTTTGGGCGTCGGCGGCGTACTGACCGATCTTGATGA
TAATGTCATCCAGCGTGCATTTAAGCTGTATCCGCTGGAGTGGATGATGCGTGACGATAACGGTCCGCTGCTGCGCAAGC
GTCGCGAGCAATGGGTGGAGCCGTTATGGAAAAGTATCTTGAGTAATAAAGGGCTAATGCCGCTGCTTTGGCGCTTCTTT
CCTGGTCATCCTAACCTTCTTGCATCCTGGTTTGAGGGCGAAAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAA
ACCGATCTACTCACGCGAAGGCGGAAACGTCACCATTTTCGACGGTCAGAATAACGTCGTTGACCACGCTGATGGTGATT
ACGCCGATGAACCGATGATCTACCAGGCGTTTCAACCTCTGCCGCGATTTGGCGATAGCTACACACTCATCGGTAGCTGG
ATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACGCTGATCACCAAAGACACCTCACGTTTCGTTCC
GCATTACATTGCTGGATAA

Upstream 100 bases:

>100_bases
CTTACTCTTCAGGCGGCTTTACCACCAAAAAAGCGTCAACCGTTTCGCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGT
GGGCATTGGGGAGGCTAATC

Downstream 100 bases:

>100_bases
GAATGTTTTAGCAATCTCTTTCTGTCAGGAATCCATGGCAGTGACCATACTAAGGGTGACTGCCATTGATGGAGGGAGAC
ACAGTGCACTGGCAAACTCA

Product: synthetase/amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIP
PLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFF
PGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSW
IVDDEACGMGIREDNTLITKDTSRFVPHYIAG

Sequences:

>Translated_352_residues
MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIP
PLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFF
PGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSW
IVDDEACGMGIREDNTLITKDTSRFVPHYIAG
>Mature_352_residues
MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIP
PLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFF
PGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSW
IVDDEACGMGIREDNTLITKDTSRFVPHYIAG

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=387, Percent_Identity=90.1808785529716, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1789416, Length=387, Percent_Identity=45.2196382428941, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1789361, Length=365, Percent_Identity=26.8493150684932, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: NA

Molecular weight: Translated: 40902; Mature: 40902

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
CCCCCCCHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHH
LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAI
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCHHHHHHHHH
QERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEEHHHHHHHHCCCCCCEEEEEECCCCCCH
VLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFF
HHHCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
PGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMI
CCCHHHHHHHHCCCCCCEECCHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHH
YQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG
HHHHHHHHCCCCCEEEEEEEEECCHHHCCCCCCCCEEEECCHHHHCCHHHCC
>Mature Secondary Structure
MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
CCCCCCCHHHHHHHHHCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHH
LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAI
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCEEEEECCCCCHHHHHHHHH
QERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGG
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHEEHHHHHHHHCCCCCCEEEEEECCCCCCH
VLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFF
HHHCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
PGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMI
CCCHHHHHHHHCCCCCCEECCHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHH
YQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG
HHHHHHHHCCCCCEEEEEEEEECCHHHCCCCCCCCEEEECCHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]