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The map label for this gene is tilS [H]

Identifier: 78780128

GI number: 78780128

Start: 1632256

End: 1633266

Strand: Direct

Name: tilS [H]

Synonym: PMT9312_1743

Alternate gene names: 78780128

Gene position: 1632256-1633266 (Clockwise)

Preceding gene: 78780125

Following gene: 78780132

Centisome position: 95.5

GC content: 28.49

Gene sequence:

>1011_bases
ATGTCTGATAAAAATTTTACTCATAAAAATTGGTCAGCATTGCATCATCTATTTCATAAAGAGCTTCTTAGCAAAAACAA
ATTAATTCCCAAAGGATCAAATATTGTAATAAGTGTTTCCGGAGGACAAGACTCAATGGCTTTATTAACACTAATTAATG
ACCTAAAAAAACTTCATAATTGGTCTATTAGTGTTTGGCATGGTGATCATCAGTGGCACGAAAAATCCTCGCTATATGCT
CTTGAATTAAAAAGTTTTTGCGAAGGTAAAAATATTTCATTCTATTTTGACCAAGCAAAAAAAGAAAATGTTTTTACAGA
AGAAAAAGCACGAGAATGGAGATATAAAAAATTATGTGAAAGAGCTAAAACTTTATTAAAAAAGGACCAGGGGGGGGGGG
ATATTTATTTGTTAACTGGTCACACGAGTAGTGATAATGCAGAAACATTTATCCTCAATTTATCTAGAGGGAGCAATTTT
ACCGGTCTGAGTAATATTGAGAGCAAAAGATTAATAGAAAATAAAATTTTTTTAATAAGACCAATATTAATTTTCAGTAG
GGAAGATACAAAACAATTTTGTAATGATATGAAGATCCCAGTCTGGGAAGATCCTACAAATTCAGATCTTAAATTAAAAA
GAAATTTAGTAAGAAAAAAAATTATTCCTACCTTAGAAGTTATCTATCCTGGTTGTTCTGAAAGGATAAATAATTTTTCC
CAAAAAATGAGCAACTACAATAATGAACGTAATGATCTTAGTGAACTAGCATACCTATATTGTAAAGATTTAAGAGGTAT
CAATAGGAATCTCTTAAATAGTATGTGTATTGAAGCAAGATGCACAATCTTAAATAGATTTTTAAAAGAAATATCTGCAA
AGCAGTGTAGTTCTAAAAATCTGACAAAATTAGCAACTTCAATTTATGAAAAAAATAAAGGTCAAATAAACTTGCAAGAG
TTTTTAAAAATTGTTTGGAATAAAAACTATATAAATTTTGAAAAAAGTTAG

Upstream 100 bases:

>100_bases
ATTAATCAGACTCATTTAATTAATGAATTTATTACCTGATTGTGACATTGTTTTAGTAAAACAGTGCATTTTTTAAAAAA
TATTATCTGAGCAACACAAA

Downstream 100 bases:

>100_bases
GATGTGACAGCAGATCTTCTTCTTCTTGTTCTACCTTCAAATGAATCTTCTGTAGCAGTCTCAGCTGATGGATTCTGTGA
AACTTTTGAACTTTTTTGAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSDKNFTHKNWSALHHLFHKELLSKNKLIPKGSNIVISVSGGQDSMALLTLINDLKKLHNWSISVWHGDHQWHEKSSLYA
LELKSFCEGKNISFYFDQAKKENVFTEEKAREWRYKKLCERAKTLLKKDQGGGDIYLLTGHTSSDNAETFILNLSRGSNF
TGLSNIESKRLIENKIFLIRPILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
QKMSNYNNERNDLSELAYLYCKDLRGINRNLLNSMCIEARCTILNRFLKEISAKQCSSKNLTKLATSIYEKNKGQINLQE
FLKIVWNKNYINFEKS

Sequences:

>Translated_336_residues
MSDKNFTHKNWSALHHLFHKELLSKNKLIPKGSNIVISVSGGQDSMALLTLINDLKKLHNWSISVWHGDHQWHEKSSLYA
LELKSFCEGKNISFYFDQAKKENVFTEEKAREWRYKKLCERAKTLLKKDQGGGDIYLLTGHTSSDNAETFILNLSRGSNF
TGLSNIESKRLIENKIFLIRPILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
QKMSNYNNERNDLSELAYLYCKDLRGINRNLLNSMCIEARCTILNRFLKEISAKQCSSKNLTKLATSIYEKNKGQINLQE
FLKIVWNKNYINFEKS
>Mature_335_residues
SDKNFTHKNWSALHHLFHKELLSKNKLIPKGSNIVISVSGGQDSMALLTLINDLKKLHNWSISVWHGDHQWHEKSSLYAL
ELKSFCEGKNISFYFDQAKKENVFTEEKAREWRYKKLCERAKTLLKKDQGGGDIYLLTGHTSSDNAETFILNLSRGSNFT
GLSNIESKRLIENKIFLIRPILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFSQ
KMSNYNNERNDLSELAYLYCKDLRGINRNLLNSMCIEARCTILNRFLKEISAKQCSSKNLTKLATSIYEKNKGQINLQEF
LKIVWNKNYINFEKS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=287, Percent_Identity=24.0418118466899, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 39128; Mature: 38997

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKNFTHKNWSALHHLFHKELLSKNKLIPKGSNIVISVSGGQDSMALLTLINDLKKLHN
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
WSISVWHGDHQWHEKSSLYALELKSFCEGKNISFYFDQAKKENVFTEEKAREWRYKKLCE
CEEEEEECCCHHHHCCCEEHEEHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
RAKTLLKKDQGGGDIYLLTGHTSSDNAETFILNLSRGSNFTGLSNIESKRLIENKIFLIR
HHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCHHEEE
PILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
HHHEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
QKMSNYNNERNDLSELAYLYCKDLRGINRNLLNSMCIEARCTILNRFLKEISAKQCSSKN
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LTKLATSIYEKNKGQINLQEFLKIVWNKNYINFEKS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCC
>Mature Secondary Structure 
SDKNFTHKNWSALHHLFHKELLSKNKLIPKGSNIVISVSGGQDSMALLTLINDLKKLHN
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
WSISVWHGDHQWHEKSSLYALELKSFCEGKNISFYFDQAKKENVFTEEKAREWRYKKLCE
CEEEEEECCCHHHHCCCEEHEEHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHH
RAKTLLKKDQGGGDIYLLTGHTSSDNAETFILNLSRGSNFTGLSNIESKRLIENKIFLIR
HHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCHHEEE
PILIFSREDTKQFCNDMKIPVWEDPTNSDLKLKRNLVRKKIIPTLEVIYPGCSERINNFS
HHHEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHH
QKMSNYNNERNDLSELAYLYCKDLRGINRNLLNSMCIEARCTILNRFLKEISAKQCSSKN
HHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LTKLATSIYEKNKGQINLQEFLKIVWNKNYINFEKS
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]