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The map label for this gene is ask [H]

Identifier: 78780125

GI number: 78780125

Start: 1627557

End: 1629317

Strand: Direct

Name: ask [H]

Synonym: PMT9312_1740

Alternate gene names: 78780125

Gene position: 1627557-1629317 (Clockwise)

Preceding gene: 78780124

Following gene: 78780128

Centisome position: 95.22

GC content: 36.06

Gene sequence:

>1761_bases
ATGGCTTTACTAGTAAAAAAATTTGGCGGGACTTCCGTTGGTGATATTAAAAAAATTCAAAATATTGCAACTAGCATTTG
TCAAAGTAAAGAAGCAGGAAATGAAATTGTCGTAGTTGTCTCTGCAATGGGCCATACTACCGATAACTTAAATTCTATAG
CGGAATCAATAAGCAAAAATCCCAATCGAAGAGAACTGGATATGCTTCTCTCAACTGGAGAGCAAGTAACCATAGCTCTT
CTCTCAATGGCATTAAATGAATACGGAATACCCGCAATTTCCATGACCGGTAGCCAGGTAGGAATTATTACTGAATCAAT
ACATGGAAAAGCAAGAATTCTGGATATTCAAACAGGAAGAATTCAAAATTATATAGATCAGGGTTTTGTAGTGGTAGTGG
CTGGATTTCAAGGGACAACATTAAGCCATACTGGATCAATGGAAATTACGACTTTGGGTAGAGGCGGTTCAGATACATCC
GCAGTAGCTTTATCAACAGCTCTAGGAGCTGAGACTTGCGAAATTTATACAGACGTTCCTGGAGTTCTTACTACTGATCC
AAGAATTGTTCCTAATGCAAAACTCTTAGATTCAATTAGTTGTGAGGAAATGCTTGAACTTGCAAGCGTCGGCGCTTCAG
TTCTTCATCCACGAGCTGTAGAAATTGCCCGCAATTATGGAATTAAATTATGCGTCAAATCAAGCCAAAGCAACTCAAGT
GGAACTCTCCTCGAAAGCCAAATCCAACCTCTCCCCCTTAAAAGAGGAAGCTTAGAATTAACAAAAACAGTAAATAGTCT
TGAAGTTTTAGAAAACCAAGCAGTATTCAGTCTCTCAAATATTCCTGATAGACCTGGGATTGCAGCTCAAATCTTTGAAA
AACTATCAGAAGCAAGTATTAATGTAGATTTAATAATACAAGCGACAAATGATGGAAATAATAACGATATTACATTTACT
GTAAGTGCATTAGAAGCTCAAAAAACTGCAGAACAATGTGAACTTATAACAAGTCAATTAGGAGGCGAATACAATTTAAA
AACAAACATGACTAAATTGAGCATTCAGGGAGCAGGCATTATGGGTAGGCCAAGTGTCTCAGCTGATTTGTTTGATACAT
TATCACAAGCGAATATCAACGTAAGGTTAATAGCTACTAGTGAAATTAAAGTCAGCTGCGTCATTGAAATTAATAATATT
CCAAAAGCCATTAGATTTGTTGCTGAGAAGTTTAAGTTATCTGATACACAAATATTCGTTAATCCAATCTACGAAAAACA
AGATCAACCTGAAGTAAGAGGAATTGCATTAGATAAAAACCAAGTACAAGTAAGTTTTCGAAAACTGCCTGATCGTCCAG
GTGTAGCAGCATCGATATGCTTAGCATTAGCTGAAAATAATTTACTTTTCGATACTATCGTGCAATCAGAGAGAATTTCC
TCTTTAAAAGCTAAGGATATTAGTCTTACCATGAATAAGCAAGATAGAGAAAAAGCTAAATTAGTTTTTGAGGCTTTAAC
CAAGAAATTACCCGGTTCATATATTGAAGATGGCCCTGCAATAGCCAAAGTAAGCACTGTAGGAGCAGGAATGGCATTTA
AGGTTGGAACAGCTGGAAAAATATTTAGAGCACTGGCCAACCAAAATATCAATATTGAAATGATTGCCACCAGTGAAATT
AGAACTTCATGTATTGTTTTAGAGAAAGATTGTGACAAAGCAGTAAATGCTATTCATAATCATTTCGAATTGGAAAAATA
A

Upstream 100 bases:

>100_bases
ACTAAAAAAAGGTAATAATCCTATAAATGTTTTTACAGAAAATTTAATTAATTTAAATTAACCAAATTAGAAGTTTAAGA
ATTTACATTATGATTTAAAT

Downstream 100 bases:

>100_bases
GTTATTTTTAGCTATTTCTTGCTAATTTTTGTCTTAATTTTTTAATTCTATCCCTCAAATTTGCTGCTTCTTCAAAATTT
AACTCTTTTGCAGCATCTTT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MALLVKKFGGTSVGDIKKIQNIATSICQSKEAGNEIVVVVSAMGHTTDNLNSIAESISKNPNRRELDMLLSTGEQVTIAL
LSMALNEYGIPAISMTGSQVGIITESIHGKARILDIQTGRIQNYIDQGFVVVVAGFQGTTLSHTGSMEITTLGRGGSDTS
AVALSTALGAETCEIYTDVPGVLTTDPRIVPNAKLLDSISCEEMLELASVGASVLHPRAVEIARNYGIKLCVKSSQSNSS
GTLLESQIQPLPLKRGSLELTKTVNSLEVLENQAVFSLSNIPDRPGIAAQIFEKLSEASINVDLIIQATNDGNNNDITFT
VSALEAQKTAEQCELITSQLGGEYNLKTNMTKLSIQGAGIMGRPSVSADLFDTLSQANINVRLIATSEIKVSCVIEINNI
PKAIRFVAEKFKLSDTQIFVNPIYEKQDQPEVRGIALDKNQVQVSFRKLPDRPGVAASICLALAENNLLFDTIVQSERIS
SLKAKDISLTMNKQDREKAKLVFEALTKKLPGSYIEDGPAIAKVSTVGAGMAFKVGTAGKIFRALANQNINIEMIATSEI
RTSCIVLEKDCDKAVNAIHNHFELEK

Sequences:

>Translated_586_residues
MALLVKKFGGTSVGDIKKIQNIATSICQSKEAGNEIVVVVSAMGHTTDNLNSIAESISKNPNRRELDMLLSTGEQVTIAL
LSMALNEYGIPAISMTGSQVGIITESIHGKARILDIQTGRIQNYIDQGFVVVVAGFQGTTLSHTGSMEITTLGRGGSDTS
AVALSTALGAETCEIYTDVPGVLTTDPRIVPNAKLLDSISCEEMLELASVGASVLHPRAVEIARNYGIKLCVKSSQSNSS
GTLLESQIQPLPLKRGSLELTKTVNSLEVLENQAVFSLSNIPDRPGIAAQIFEKLSEASINVDLIIQATNDGNNNDITFT
VSALEAQKTAEQCELITSQLGGEYNLKTNMTKLSIQGAGIMGRPSVSADLFDTLSQANINVRLIATSEIKVSCVIEINNI
PKAIRFVAEKFKLSDTQIFVNPIYEKQDQPEVRGIALDKNQVQVSFRKLPDRPGVAASICLALAENNLLFDTIVQSERIS
SLKAKDISLTMNKQDREKAKLVFEALTKKLPGSYIEDGPAIAKVSTVGAGMAFKVGTAGKIFRALANQNINIEMIATSEI
RTSCIVLEKDCDKAVNAIHNHFELEK
>Mature_585_residues
ALLVKKFGGTSVGDIKKIQNIATSICQSKEAGNEIVVVVSAMGHTTDNLNSIAESISKNPNRRELDMLLSTGEQVTIALL
SMALNEYGIPAISMTGSQVGIITESIHGKARILDIQTGRIQNYIDQGFVVVVAGFQGTTLSHTGSMEITTLGRGGSDTSA
VALSTALGAETCEIYTDVPGVLTTDPRIVPNAKLLDSISCEEMLELASVGASVLHPRAVEIARNYGIKLCVKSSQSNSSG
TLLESQIQPLPLKRGSLELTKTVNSLEVLENQAVFSLSNIPDRPGIAAQIFEKLSEASINVDLIIQATNDGNNNDITFTV
SALEAQKTAEQCELITSQLGGEYNLKTNMTKLSIQGAGIMGRPSVSADLFDTLSQANINVRLIATSEIKVSCVIEINNIP
KAIRFVAEKFKLSDTQIFVNPIYEKQDQPEVRGIALDKNQVQVSFRKLPDRPGVAASICLALAENNLLFDTIVQSERISS
LKAKDISLTMNKQDREKAKLVFEALTKKLPGSYIEDGPAIAKVSTVGAGMAFKVGTAGKIFRALANQNINIEMIATSEIR
TSCIVLEKDCDKAVNAIHNHFELEK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=354, Percent_Identity=31.9209039548023, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790455, Length=359, Percent_Identity=30.08356545961, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790376, Length=289, Percent_Identity=25.2595155709343, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=409, Percent_Identity=25.4278728606357, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 62953; Mature: 62822

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLVKKFGGTSVGDIKKIQNIATSICQSKEAGNEIVVVVSAMGHTTDNLNSIAESISKN
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
PNRRELDMLLSTGEQVTIALLSMALNEYGIPAISMTGSQVGIITESIHGKARILDIQTGR
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCEEEEEEECCH
IQNYIDQGFVVVVAGFQGTTLSHTGSMEITTLGRGGSDTSAVALSTALGAETCEIYTDVP
HHHHHCCCEEEEEECCCCCEEECCCCEEEEEECCCCCCCHHEEEHHHCCCCEEEEEECCC
GVLTTDPRIVPNAKLLDSISCEEMLELASVGASVLHPRAVEIARNYGIKLCVKSSQSNSS
CCEECCCCCCCCHHHHHCCCHHHHHHHHHCCHHHCCCHHHHHHHCCCEEEEEECCCCCCC
GTLLESQIQPLPLKRGSLELTKTVNSLEVLENQAVFSLSNIPDRPGIAAQIFEKLSEASI
CCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCC
NVDLIIQATNDGNNNDITFTVSALEAQKTAEQCELITSQLGGEYNLKTNMTKLSIQGAGI
CEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCC
MGRPSVSADLFDTLSQANINVRLIATSEIKVSCVIEINNIPKAIRFVAEKFKLSDTQIFV
CCCCCCCHHHHHHHHHCCCEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCCCEEEE
NPIYEKQDQPEVRGIALDKNQVQVSFRKLPDRPGVAASICLALAENNLLFDTIVQSERIS
EEHHCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHH
SLKAKDISLTMNKQDREKAKLVFEALTKKLPGSYIEDGPAIAKVSTVGAGMAFKVGTAGK
HCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCHHH
IFRALANQNINIEMIATSEIRTSCIVLEKDCDKAVNAIHNHFELEK
HHHHHHCCCCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALLVKKFGGTSVGDIKKIQNIATSICQSKEAGNEIVVVVSAMGHTTDNLNSIAESISKN
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
PNRRELDMLLSTGEQVTIALLSMALNEYGIPAISMTGSQVGIITESIHGKARILDIQTGR
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCEEEEEEECCH
IQNYIDQGFVVVVAGFQGTTLSHTGSMEITTLGRGGSDTSAVALSTALGAETCEIYTDVP
HHHHHCCCEEEEEECCCCCEEECCCCEEEEEECCCCCCCHHEEEHHHCCCCEEEEEECCC
GVLTTDPRIVPNAKLLDSISCEEMLELASVGASVLHPRAVEIARNYGIKLCVKSSQSNSS
CCEECCCCCCCCHHHHHCCCHHHHHHHHHCCHHHCCCHHHHHHHCCCEEEEEECCCCCCC
GTLLESQIQPLPLKRGSLELTKTVNSLEVLENQAVFSLSNIPDRPGIAAQIFEKLSEASI
CCEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCC
NVDLIIQATNDGNNNDITFTVSALEAQKTAEQCELITSQLGGEYNLKTNMTKLSIQGAGI
CEEEEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCCC
MGRPSVSADLFDTLSQANINVRLIATSEIKVSCVIEINNIPKAIRFVAEKFKLSDTQIFV
CCCCCCCHHHHHHHHHCCCEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCCCEEEE
NPIYEKQDQPEVRGIALDKNQVQVSFRKLPDRPGVAASICLALAENNLLFDTIVQSERIS
EEHHCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHH
SLKAKDISLTMNKQDREKAKLVFEALTKKLPGSYIEDGPAIAKVSTVGAGMAFKVGTAGK
HCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEECCHHH
IFRALANQNINIEMIATSEIRTSCIVLEKDCDKAVNAIHNHFELEK
HHHHHHCCCCEEEEEEECCCCEEEEEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10074061 [H]