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The map label for this gene is acoA [H]

Identifier: 78779685

GI number: 78779685

Start: 1209543

End: 1210586

Strand: Direct

Name: acoA [H]

Synonym: PMT9312_1302

Alternate gene names: 78779685

Gene position: 1209543-1210586 (Clockwise)

Preceding gene: 78779684

Following gene: 78779686

Centisome position: 70.77

GC content: 32.47

Gene sequence:

>1044_bases
ATGAAAGATTCCAATAGTAAAGAAATAGATTCCATAACATCTGAAAAGGAAATTAATATCGATAATTTCTCTAGAGATGA
ATTATTGGAAATGCTTTCAAAAATGATATTAATAAGAAATGCAGAATATAAAATTGCCAAAGGACGAGAATTTGGATTAG
TTGGTGGTCCTGTACATTTAGGGGTTGGTCAAGAAGCTATACCAGTAGGTATCTCTCAATATTTAAATAATCAGGATAAA
GTATTTGGGGCCCATAGATCTCATTCTCATATACTTTCCTTAGGTATAGATCTTAAATCATTCTTTTCTGAAATACTTGC
AAAATCCTCAGGAATATCAAAGGGGATGGGTGGTTCAATGCATCTTTTTGGAGGTTCAGTAGGTTTTTGTGGATCTGTAC
CAATAGTTGGAGGAACAGTTCCTCTTGCCGTTGGAACGGCTTTAGCTAGTAAGTTAAAAGAAGAAAAAGTTGTATCTATT
TCTTATTTAGGCGATGGCGCAATAGAAGAAGGCATAGTTCACGAAAGTTTAAATTTTGCAAGAATTAATAATTGTCCCGT
TATTTTTGTAGTCGAAAATAACTTATTCTCAAGTCATTTAAATATAAAGTTGAGGCAGCCCAAAAAGTTAACGTATAGAT
TTGCGAAAGCAAATGATATTGAGTCAAAAGTATTAGATGGAAACAACTTAACTTCTATTTGCAAAACGGGCAAAGAATTT
ATTAGAAGATGTAGAGATGGGGAGGGGCCATTTTTCATAGAAGCACTTACATATAGATGGTTTGGTCATGTCGATTGGAG
AGAAGATATTGATGTTGGAATAAATAGATCTGCAGATGATTTAAAATATTGGAAGAAAAGAGATCCAATTTTAAGGTTAA
AAAAATCACTTTTAAAAGAAAATTATTTTGGAGAGAATCATTTGATAAACTTAGAGAAAGATATTCAGAAAGATATAGAT
AACGCATGGGTCGATGCTTTAGGAGAATCTTCCCCAGAATGGAACGAATCCCTAGATTATGTTTTTAAAAGCAATTACAA
ATGA

Upstream 100 bases:

>100_bases
GTCTTTATAAGAGCAAAGAATAATAAAAGCAAGGCAATTATTATGAATAGTGCAGATTTTTAGTTTATGATTAAATAAAT
TTGGATGCGTTTATAAATAT

Downstream 100 bases:

>100_bases
AAAAGTTTACATATAGCACTGCAATTCTAGATGCATATAATTTTTTATTAAAAAATTATCCTGAAGTATTCGTAATTGGA
CAAGGTTTATGGAGTCCTTG

Product: pyruvate dehydrogenase (lipoamide)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKDSNSKEIDSITSEKEINIDNFSRDELLEMLSKMILIRNAEYKIAKGREFGLVGGPVHLGVGQEAIPVGISQYLNNQDK
VFGAHRSHSHILSLGIDLKSFFSEILAKSSGISKGMGGSMHLFGGSVGFCGSVPIVGGTVPLAVGTALASKLKEEKVVSI
SYLGDGAIEEGIVHESLNFARINNCPVIFVVENNLFSSHLNIKLRQPKKLTYRFAKANDIESKVLDGNNLTSICKTGKEF
IRRCRDGEGPFFIEALTYRWFGHVDWREDIDVGINRSADDLKYWKKRDPILRLKKSLLKENYFGENHLINLEKDIQKDID
NAWVDALGESSPEWNESLDYVFKSNYK

Sequences:

>Translated_347_residues
MKDSNSKEIDSITSEKEINIDNFSRDELLEMLSKMILIRNAEYKIAKGREFGLVGGPVHLGVGQEAIPVGISQYLNNQDK
VFGAHRSHSHILSLGIDLKSFFSEILAKSSGISKGMGGSMHLFGGSVGFCGSVPIVGGTVPLAVGTALASKLKEEKVVSI
SYLGDGAIEEGIVHESLNFARINNCPVIFVVENNLFSSHLNIKLRQPKKLTYRFAKANDIESKVLDGNNLTSICKTGKEF
IRRCRDGEGPFFIEALTYRWFGHVDWREDIDVGINRSADDLKYWKKRDPILRLKKSLLKENYFGENHLINLEKDIQKDID
NAWVDALGESSPEWNESLDYVFKSNYK
>Mature_347_residues
MKDSNSKEIDSITSEKEINIDNFSRDELLEMLSKMILIRNAEYKIAKGREFGLVGGPVHLGVGQEAIPVGISQYLNNQDK
VFGAHRSHSHILSLGIDLKSFFSEILAKSSGISKGMGGSMHLFGGSVGFCGSVPIVGGTVPLAVGTALASKLKEEKVVSI
SYLGDGAIEEGIVHESLNFARINNCPVIFVVENNLFSSHLNIKLRQPKKLTYRFAKANDIESKVLDGNNLTSICKTGKEF
IRRCRDGEGPFFIEALTYRWFGHVDWREDIDVGINRSADDLKYWKKRDPILRLKKSLLKENYFGENHLINLEKDIQKDID
NAWVDALGESSPEWNESLDYVFKSNYK

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=325, Percent_Identity=29.2307692307692, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI291084742, Length=325, Percent_Identity=28, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4505685, Length=325, Percent_Identity=28, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI291084744, Length=332, Percent_Identity=27.4096385542169, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI258645172, Length=323, Percent_Identity=28.1733746130031, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI11386135, Length=324, Percent_Identity=28.0864197530864, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI291084757, Length=325, Percent_Identity=24, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536047, Length=303, Percent_Identity=29.7029702970297, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564172, Length=303, Percent_Identity=29.7029702970297, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI86563355, Length=265, Percent_Identity=28.6792452830189, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI86563357, Length=265, Percent_Identity=28.6792452830189, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321026, Length=331, Percent_Identity=32.02416918429, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24639748, Length=325, Percent_Identity=29.5384615384615, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639744, Length=300, Percent_Identity=27, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28571106, Length=300, Percent_Identity=27, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24639740, Length=300, Percent_Identity=27, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24639746, Length=288, Percent_Identity=27.4305555555556, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21355903, Length=335, Percent_Identity=23.2835820895522, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 38821; Mature: 38821

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSNSKEIDSITSEKEINIDNFSRDELLEMLSKMILIRNAEYKIAKGREFGLVGGPVHL
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEECEECCCEEE
GVGQEAIPVGISQYLNNQDKVFGAHRSHSHILSLGIDLKSFFSEILAKSSGISKGMGGSM
CCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEE
HLFGGSVGFCGSVPIVGGTVPLAVGTALASKLKEEKVVSISYLGDGAIEEGIVHESLNFA
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHCHHHHCCCEE
RINNCPVIFVVENNLFSSHLNIKLRQPKKLTYRFAKANDIESKVLDGNNLTSICKTGKEF
EECCCCEEEEECCCEECCCCEEEEECCHHHEEEECCCCCCHHHHCCCCCHHHHHHHHHHH
IRRCRDGEGPFFIEALTYRWFGHVDWREDIDVGINRSADDLKYWKKRDPILRLKKSLLKE
HHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
NYFGENHLINLEKDIQKDIDNAWVDALGESSPEWNESLDYVFKSNYK
CCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDSNSKEIDSITSEKEINIDNFSRDELLEMLSKMILIRNAEYKIAKGREFGLVGGPVHL
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCEECEECCCEEE
GVGQEAIPVGISQYLNNQDKVFGAHRSHSHILSLGIDLKSFFSEILAKSSGISKGMGGSM
CCCCCHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEE
HLFGGSVGFCGSVPIVGGTVPLAVGTALASKLKEEKVVSISYLGDGAIEEGIVHESLNFA
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHCHHHHCCCEE
RINNCPVIFVVENNLFSSHLNIKLRQPKKLTYRFAKANDIESKVLDGNNLTSICKTGKEF
EECCCCEEEEECCCEECCCCEEEEECCHHHEEEECCCCCCHHHHCCCCCHHHHHHHHHHH
IRRCRDGEGPFFIEALTYRWFGHVDWREDIDVGINRSADDLKYWKKRDPILRLKKSLLKE
HHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
NYFGENHLINLEKDIQKDIDNAWVDALGESSPEWNESLDYVFKSNYK
CCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]