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The map label for this gene is pdhB [H]

Identifier: 78779686

GI number: 78779686

Start: 1210583

End: 1211572

Strand: Direct

Name: pdhB [H]

Synonym: PMT9312_1303

Alternate gene names: 78779686

Gene position: 1210583-1211572 (Clockwise)

Preceding gene: 78779685

Following gene: 78779687

Centisome position: 70.83

GC content: 33.13

Gene sequence:

>990_bases
ATGAAAAAGTTTACATATAGCACTGCAATTCTAGATGCATATAATTTTTTATTAAAAAATTATCCTGAAGTATTCGTAAT
TGGACAAGGTTTATGGAGTCCTTGGTATGTGGGTAATACTATGAAAGATCTAGATAAAAATTTTGGAAAAAAAAGAATTA
TCGATACTCCAGTATCAGAAGCTGCGGTAACAGGGGCCGCCGTTGGAGCCTCACTTAATGAAATGAAGCCAATAGTTGTT
CATCCAAGAATGGATTTTATGATGTACGCAATGGATCCAATAATTAATCAAGCTGCAAAATGGTCTTATATGTTTGGCGG
GCAATCAAGTCCATCTATTACTATAAGAGGAATAATAAATAGAGGAGGAGAACAAGGGGCACAACACTCACAAGCCTTAC
ATTCTTTGTTCGCACACATACCAGGTTTAAAAGTAGTCTTGCCATCATCAGTGGCTGATGCTAGAGATCTTTTAATAGCC
TCTGTTTTAGCTGATCAACCAGTAATTTATATAGATGACAGATGGCTTTACGATCAAGAAGATCAACTCCCTGAAGCGAA
AGAAATCAACCTAGAAAGTATAAATCCTTGTATTTTGCGAGAAGGCAATTCAATAACATTAGTTGGATGTAGTTACTCAA
CATTTTTACTTAAACAAATAACTAAGAAATTAATTAAAAATAAAATTAATCCTGAAATCATTGATATGAGAATAATTAAT
CCTTTTCATAGTGAATTAATTACTAATTCAGTCAAGAAAACTGGCCGTTTATTTGTGCTGGATGGAGGATGGGGACCTTG
TGGTATATCATCTGAGATAATTTCTTCCGCTGTCGAGAATGTCGAACCTAAATTTTTCAAATCCAAACCTGCGAGGTTAA
CTCTACCTTTTACACCCGCACCAACAAGCAAAGTTTTGGAAAAAGAATACTATCCAAATGAGAAAAAAATATTAAATAAA
ATTTTTAAAATTTTTGAAAATAATTTATAA

Upstream 100 bases:

>100_bases
ATATTCAGAAAGATATAGATAACGCATGGGTCGATGCTTTAGGAGAATCTTCCCCAGAATGGAACGAATCCCTAGATTAT
GTTTTTAAAAGCAATTACAA

Downstream 100 bases:

>100_bases
TTATTTCTATGAAAATTCCATTCTTCGATTACCCAAGACTTTTTTTAGATAAAAAAGATGAATATATGAATATCTTCTCA
GATGTAGCTTCAAGAGGGGC

Product: pyruvate dehydrogenase (lipoamide)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKFTYSTAILDAYNFLLKNYPEVFVIGQGLWSPWYVGNTMKDLDKNFGKKRIIDTPVSEAAVTGAAVGASLNEMKPIVV
HPRMDFMMYAMDPIINQAAKWSYMFGGQSSPSITIRGIINRGGEQGAQHSQALHSLFAHIPGLKVVLPSSVADARDLLIA
SVLADQPVIYIDDRWLYDQEDQLPEAKEINLESINPCILREGNSITLVGCSYSTFLLKQITKKLIKNKINPEIIDMRIIN
PFHSELITNSVKKTGRLFVLDGGWGPCGISSEIISSAVENVEPKFFKSKPARLTLPFTPAPTSKVLEKEYYPNEKKILNK
IFKIFENNL

Sequences:

>Translated_329_residues
MKKFTYSTAILDAYNFLLKNYPEVFVIGQGLWSPWYVGNTMKDLDKNFGKKRIIDTPVSEAAVTGAAVGASLNEMKPIVV
HPRMDFMMYAMDPIINQAAKWSYMFGGQSSPSITIRGIINRGGEQGAQHSQALHSLFAHIPGLKVVLPSSVADARDLLIA
SVLADQPVIYIDDRWLYDQEDQLPEAKEINLESINPCILREGNSITLVGCSYSTFLLKQITKKLIKNKINPEIIDMRIIN
PFHSELITNSVKKTGRLFVLDGGWGPCGISSEIISSAVENVEPKFFKSKPARLTLPFTPAPTSKVLEKEYYPNEKKILNK
IFKIFENNL
>Mature_329_residues
MKKFTYSTAILDAYNFLLKNYPEVFVIGQGLWSPWYVGNTMKDLDKNFGKKRIIDTPVSEAAVTGAAVGASLNEMKPIVV
HPRMDFMMYAMDPIINQAAKWSYMFGGQSSPSITIRGIINRGGEQGAQHSQALHSLFAHIPGLKVVLPSSVADARDLLIA
SVLADQPVIYIDDRWLYDQEDQLPEAKEINLESINPCILREGNSITLVGCSYSTFLLKQITKKLIKNKINPEIIDMRIIN
PFHSELITNSVKKTGRLFVLDGGWGPCGISSEIISSAVENVEPKFFKSKPARLTLPFTPAPTSKVLEKEYYPNEKKILNK
IFKIFENNL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=32.4074074074074, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=31.1728395061728, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=28.7461773700306, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=28.7461773700306, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17538422, Length=325, Percent_Identity=32, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17506935, Length=287, Percent_Identity=28.9198606271777, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319698, Length=333, Percent_Identity=31.8318318318318, Blast_Score=163, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21358145, Length=331, Percent_Identity=30.8157099697885, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24650940, Length=331, Percent_Identity=30.8157099697885, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI160714828, Length=285, Percent_Identity=29.1228070175439, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI160714832, Length=288, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36815; Mature: 36815

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFTYSTAILDAYNFLLKNYPEVFVIGQGLWSPWYVGNTMKDLDKNFGKKRIIDTPVSE
CCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCHHHHHHHCCCCCEECCCCCHH
AAVTGAAVGASLNEMKPIVVHPRMDFMMYAMDPIINQAAKWSYMFGGQSSPSITIRGIIN
HHHHHHHHCCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEEEC
RGGEQGAQHSQALHSLFAHIPGLKVVLPSSVADARDLLIASVLADQPVIYIDDRWLYDQE
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCEECCCH
DQLPEAKEINLESINPCILREGNSITLVGCSYSTFLLKQITKKLIKNKINPEIIDMRIIN
HCCCCHHCCCCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
PFHSELITNSVKKTGRLFVLDGGWGPCGISSEIISSAVENVEPKFFKSKPARLTLPFTPA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHCCCCCEEEEECCCC
PTSKVLEKEYYPNEKKILNKIFKIFENNL
CHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKFTYSTAILDAYNFLLKNYPEVFVIGQGLWSPWYVGNTMKDLDKNFGKKRIIDTPVSE
CCCEEHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCHHHHHHHCCCCCEECCCCCHH
AAVTGAAVGASLNEMKPIVVHPRMDFMMYAMDPIINQAAKWSYMFGGQSSPSITIRGIIN
HHHHHHHHCCCHHHCCCEEECCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEEEC
RGGEQGAQHSQALHSLFAHIPGLKVVLPSSVADARDLLIASVLADQPVIYIDDRWLYDQE
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCEECCCH
DQLPEAKEINLESINPCILREGNSITLVGCSYSTFLLKQITKKLIKNKINPEIIDMRIIN
HCCCCHHCCCCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
PFHSELITNSVKKTGRLFVLDGGWGPCGISSEIISSAVENVEPKFFKSKPARLTLPFTPA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHCCCCCEEEEECCCC
PTSKVLEKEYYPNEKKILNKIFKIFENNL
CHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]