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The map label for this gene is glnA [H]

Identifier: 78779264

GI number: 78779264

Start: 811714

End: 813135

Strand: Reverse

Name: glnA [H]

Synonym: PMT9312_0880

Alternate gene names: 78779264

Gene position: 813135-811714 (Counterclockwise)

Preceding gene: 78779266

Following gene: 78779263

Centisome position: 47.57

GC content: 36.08

Gene sequence:

>1422_bases
ATGTCTAAATCTCCTCAAGATGTTTTAAGTCAAATTAAAGACGAAGGAATTGAACTCATCGATTTAAAATTCACAGATAT
TCATGGAAAATGGCAACATTTAACACTTACATCAGACATGATAGAAGAGGATTCTTTTACTGAAGGCTTAGCGTTTGATG
GGTCATCAATAAGAGGTTGGAAAGCAATTAATGCATCTGATATGTCAATGGTGCCTGATTCAAGTACAGCTTGGATCGAT
CCTTTTTATAAACATAAAACATTAAGTATGATTTGCTCTATTCAAGAGCCAAGAAGCGGTGAGCCTTATGATAGATGTCC
AAGGGCTTTAGCTCAAAAGGCATTAAAATATTTAGACTCTACTGGCATAGCAGATACTGCATTTTTTGGACCTGAGCCAG
AATTCTTTTTATTTGATGATGTTAGATATGACTCTAAAGAAGGAGGTTGTTTTTATAGTGTAGATACTATTGAAGCACCA
TGGAATACAGGGAGAATAGAAGAAGGTGGGAACTTAGGATACAAAATACAATATAAAGAAGGATATTTTCCAGTAGCTCC
AAATGATACTGCGCAAGATATCAGATCTGAGATGCTTCTTCTTATGGGTGAATTAGGTATCCCTACAGAAAAACATCACC
ATGAAGTTGCTGGTGCCGGCCAACACGAGCTTGGAATGAAATTTGATTCTTTAATAAAAGCTGCTGATAACGTTATGACG
TATAAATACGTGGTTAGAAACGTAGCTAAAAAATATGGGAAAACTGCAACATTTATGCCTAAGCCTGTATTTAACGATAA
TGGGACTGGAATGCATGTTCACCAAAGTTTATGGAAGAGTGGACAGCCACTATTCTTTGGTGAAAGTGCCTATGCAAATT
TATCCCAAACAGCTAGATGGTATATCGGAGGAATACTTAAACATGCCCCTTCATTCCTAGCGTTTACTAATCCAACAACA
AATAGTTATAAAAGATTGGTTCCAGGATTTGAAGCACCTGTAAATCTAGTTTATTCTGAGGGTAATAGATCGGCTGCAGT
AAGAATACCTTTAACTGGTCCAAGTCCAAAAGCTAAAAGATTAGAATTTAGATCAGGTGACGCACTTGCTAACCCATATT
TAGCTTTCTCTGTAATGATGCTCGCTGGTATTGATGGGATCAAAAATCAAATAGATCCTGGTGATGGTGTAGATGTTGAT
TTATTCGAACTTCCTGCAGAAGAACTTGCAAAAATTGATACAGTACCTTCATCTCTTAATGATTCACTTAATGCACTCAA
AGCAGACAAGGATTATCTATTAGCTGGTGGAGTATTTACAGAAGATTTTATTGATAACTTTATCGATATAAAATACGAAG
AGGTACAACAATTAAGACAGAGGCCTCATCCACATGAATTCTTTATGTATTACGATGCATAA

Upstream 100 bases:

>100_bases
TTGAAACACTGAAAGAAGTCATCTAGTCTTAAAAAAAGTTACCGTTGATACATAACAAGAATCATCAAGTTATTAATTTC
ATATAAGGTATTAAAAAATT

Downstream 100 bases:

>100_bases
TTAAAAAATCTTTTCTATAAAATAAGTCAATTTGAGAAATATCACAAAATATTCTCAAATTGATTTTTTTTTTGTTGGAA
TATATATATATATATTTAAA

Product: glutamine synthetase, glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGWKAINASDMSMVPDSSTAWID
PFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDSTGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAP
WNTGRIEEGGNLGYKIQYKEGYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLIKAADNVMT
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKSGQPLFFGESAYANLSQTARWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVD
LFELPAEELAKIDTVPSSLNDSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA

Sequences:

>Translated_473_residues
MSKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGWKAINASDMSMVPDSSTAWID
PFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDSTGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAP
WNTGRIEEGGNLGYKIQYKEGYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLIKAADNVMT
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKSGQPLFFGESAYANLSQTARWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVD
LFELPAEELAKIDTVPSSLNDSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA
>Mature_472_residues
SKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGWKAINASDMSMVPDSSTAWIDP
FYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDSTGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAPW
NTGRIEEGGNLGYKIQYKEGYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLIKAADNVMTY
KYVVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKSGQPLFFGESAYANLSQTARWYIGGILKHAPSFLAFTNPTTN
SYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVDL
FELPAEELAKIDTVPSSLNDSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=494, Percent_Identity=23.6842105263158, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=53.6324786324786, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI87081870, Length=177, Percent_Identity=31.638418079096, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52810; Mature: 52679

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGW
CCCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECHHHHCCCCCCCCEEECCCCCCCE
KAINASDMSMVPDSSTAWIDPFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDS
EEECCCCCEECCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHC
TGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAPWNTGRIEEGGNLGYKIQYKE
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCEEEEECC
GYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLIKAADNVMT
CEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHH
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKSGQPLFFGESAYANLSQTARW
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHHCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKR
HHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHE
LEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVDLFELPAEELAKIDTVPSSLN
EEECCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCEEHHHHCCHHHHHHHHCCCHHHH
DSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA
HHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
SKSPQDVLSQIKDEGIELIDLKFTDIHGKWQHLTLTSDMIEEDSFTEGLAFDGSSIRGW
CCCHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECHHHHCCCCCCCCEEECCCCCCCE
KAINASDMSMVPDSSTAWIDPFYKHKTLSMICSIQEPRSGEPYDRCPRALAQKALKYLDS
EEECCCCCEECCCCCCCCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHC
TGIADTAFFGPEPEFFLFDDVRYDSKEGGCFYSVDTIEAPWNTGRIEEGGNLGYKIQYKE
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCEEEEECC
GYFPVAPNDTAQDIRSEMLLLMGELGIPTEKHHHEVAGAGQHELGMKFDSLIKAADNVMT
CEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHCCHHHHHHHHHHHHHH
YKYVVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKSGQPLFFGESAYANLSQTARW
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCEEECCHHHCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSEGNRSAAVRIPLTGPSPKAKR
HHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHE
LEFRSGDALANPYLAFSVMMLAGIDGIKNQIDPGDGVDVDLFELPAEELAKIDTVPSSLN
EEECCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCEEHHHHCCHHHHHHHHCCCHHHH
DSLNALKADKDYLLAGGVFTEDFIDNFIDIKYEEVQQLRQRPHPHEFFMYYDA
HHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678591 [H]