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The map label for this gene is topA [H]

Identifier: 78778820

GI number: 78778820

Start: 410348

End: 412954

Strand: Direct

Name: topA [H]

Synonym: PMT9312_0435

Alternate gene names: 78778820

Gene position: 410348-412954 (Clockwise)

Preceding gene: 78778817

Following gene: 78778821

Centisome position: 24.01

GC content: 35.37

Gene sequence:

>2607_bases
TTGGATCACACACTAGTTATTGTTGAAAGTCCCACTAAAGCAAAAACTATAAGAAAGTTTTTGCCTTCTAATTATGAAGT
TCTTGCTTCAATGGGCCACGTAAGAGATCTTCCAAAAGGGGCGGCTGAAATCCCTGCTGCAGTTAAAAAGGAAAAATGGT
CAAGGATAGGAGTAAATACAACAGAAGATTTTGAACCACTTTATATAGTTCCAAAAGACAAGAAAAAGGTTGTTAAAGAG
TTGAAAAATGCATTAAAAGGTGCGACCCAACTACTATTGGCAACTGATGAAGACAGAGAGGGAGAGAGTATTAGCTGGCA
TCTTTTGCAAATACTTAAGCCTAAAATACCTACTAAGAGAATGGTTTTTCACGAAATTACAAAAAAGGCAATTAATAAAG
CGTTAGATCAAACTAGAGAAATTGACATGGAACTTGTTCAAGCTCAAGAAACTAGAAGAATCTTGGATAGGCTTTTTGGA
TATGAATTATCTCCACTACTTTGGAAGAAAGTAGCTCCCAGATTATCTGCTGGTCGCGTTCAATCAGTATCTGTAAGACT
TCTTGTTAGAAGAGAGAGAGAAAGAAGATCCTTTAAAAAAGCTAGTTACTGGGGGATTAAAGCTTCTTTAGAGAAAGATA
ATATTACTTTCGAAACTAGATTATTTAGTTTGAAAGGGCAAAGAATTTCCAATGGTTCAGATTTTGATGAACAGACTGGC
AAATTAAAACGAGGAAATAAATCATTGATAATTAGAGAAGAAAAGGTAAATGAATTGTTAAAGGATTTTTCTTCTGAGGA
TTGGTTAGTGTCAAAAATTGAAAAAAAACCATCCATTCGTAAGCCAGTACCTCCATTTACAACTAGTACATTGCAACAAG
AAGCAAACAGGAAACTTCGCTTGTCTGCAAGAGAAACCATGAGATGTGCACAAGGTCTATATGAGAGAGGTTTCATAACA
TATATGAGAACTGATTCAGTGCATCTTTCCGAACAAGCTACAAGAGCTGCTAGAGAATGTGTAAGTTCCATGTATGGGAA
AGAATACTTATCTAATTCACCAAGACAATTTAATTCAACTGCAAGAAACGCTCAAGAAGCACACGAAGCTATTAGGCCGG
CAGGTGAGGTATTTAAAACACCAAAGGAAACTAATTTAACTGGTAGAGACTTATCTCTTTACGATTTAGTTTGGAAAAGA
ACTGTAGCTAGTCAAATGGCTGAAGCTAGGCTAACAATGATTAATGCTGAAATCAGTGTAGGGGATGGCTTATTTAAATC
GAGCGGGAAAAGTATTGATTTTGCAGGATTCTTCAGAGCTTATGTGGAGGGAAGTGATGACCCGAGTTCATCCCTTGAAC
AACAAGAAATAATTCTTCCAAACTTAACAACTGGAACATGTCTTGAAGTTACTGATAAGGAACCTACTTTTCATGAAACT
AAACCACCTGCAAGATATACAGAGGCTGCATTAGTTAAAGTCCTTGAAAAAGAGGGGATTGGAAGACCTTCTACCTATGC
AAGCATTATTGGGACAATTGTGGATAGAGGTTATGCAAATATATCTTCCAATACTTTGGCTCCAACGTTTACAGCTTTTG
CCGTTACCGCTCTCTTAGAAGAACATTTCCCTGATCTAGTTGATACTACTTTTACTGCAAAAATGGAATCTTCATTGGAT
GAAATATCCTCAGGTAATCTTGAGTGGCTACCATACTTAGAGACTTTTTATAAAGGTAAAAATGGTCTGGAGGTGAAAGT
TCAGAAAACAGAGGGTGACATTGATGGTAAAGCTTATAGACAAGTTGATTTCGAAGACCTCCCTTGCGTAGTTAGAATAG
GTTCTAACGGACCTTGGCTTGAGGGTATAAAAATTGATGAATCTGGTAATGAAATCCAGGCGAAAGGGAATCTTCCAATG
GATATTACTCCTGGAGATTTAGATAAAAAGCAAGTTGATCAAATTTTAAGTGGCCCATCAGATCTTGGAACTGATCCAAA
AACTGGGGAAAAAGTATTTTTAAGATTTGGACCTTATGGACCTTACGTCCAATTGGGAAATAATGATCAGGATAAAGCTA
AACCAAGAAGAGCTTCATTACCCAAAGAGTTGAAAACTGATGATTTAACTCTAGATGAGGCTCTAGTACTTTTAAGTTTG
CCTAGATTACTAGGAGTTCATCCTGAAGGAGGTATTGTTGAGGCAGATAGGGGAAGATTTGGACCTTATATTAAATGGAT
TAAAAATGAAAATGAGTCTGAAAATAGATCATTAAAAAAAGAGGATGATGTTTTTACAGTTGATTTAAAACGAGCATTAG
AAATCCTTGCGATGCCAAAAATGGGAAGAGGTGGTCAAGAGGTACTTAAAGACTTTGGAAAACCAAAAGAATTTAAAGAT
AAAATCCAAATATTAAATGGAAGATATGGAGTCTATTTAAAATGTGGCAAAACTAATGTTTCGCTTGCAAAAGATACTGA
TTTAGAGAAAATCTCAATAGATGATGCTGTATTGCTTCTGGATGAAAAACTAAAAGATAAAAAAGGATCTATTTCAAAAA
AAATAAAAATAAGTAATAAAAAAATTGCAAGAAAAAAGAAAAGTTAA

Upstream 100 bases:

>100_bases
GGTATGATTTTTGAAATTTTCAGGAGAAATTACTTGAAAAAGTTTCAAATAATCATAATATGCCCTAACTGAACAAAAAC
ACCAATTTTTGAAATAAACC

Downstream 100 bases:

>100_bases
AAATATGATTTTAAAAAAAAAAGAATTTTTTTTATTAATTTTTTTAATTTTAATGCAATCATGCTCAGGAGGGAGAATTG
GGAATTTTCTTGAAAGTAGT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 868; Mature: 868

Protein sequence:

>868_residues
MDHTLVIVESPTKAKTIRKFLPSNYEVLASMGHVRDLPKGAAEIPAAVKKEKWSRIGVNTTEDFEPLYIVPKDKKKVVKE
LKNALKGATQLLLATDEDREGESISWHLLQILKPKIPTKRMVFHEITKKAINKALDQTREIDMELVQAQETRRILDRLFG
YELSPLLWKKVAPRLSAGRVQSVSVRLLVRRERERRSFKKASYWGIKASLEKDNITFETRLFSLKGQRISNGSDFDEQTG
KLKRGNKSLIIREEKVNELLKDFSSEDWLVSKIEKKPSIRKPVPPFTTSTLQQEANRKLRLSARETMRCAQGLYERGFIT
YMRTDSVHLSEQATRAARECVSSMYGKEYLSNSPRQFNSTARNAQEAHEAIRPAGEVFKTPKETNLTGRDLSLYDLVWKR
TVASQMAEARLTMINAEISVGDGLFKSSGKSIDFAGFFRAYVEGSDDPSSSLEQQEIILPNLTTGTCLEVTDKEPTFHET
KPPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNTLAPTFTAFAVTALLEEHFPDLVDTTFTAKMESSLD
EISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYRQVDFEDLPCVVRIGSNGPWLEGIKIDESGNEIQAKGNLPM
DITPGDLDKKQVDQILSGPSDLGTDPKTGEKVFLRFGPYGPYVQLGNNDQDKAKPRRASLPKELKTDDLTLDEALVLLSL
PRLLGVHPEGGIVEADRGRFGPYIKWIKNENESENRSLKKEDDVFTVDLKRALEILAMPKMGRGGQEVLKDFGKPKEFKD
KIQILNGRYGVYLKCGKTNVSLAKDTDLEKISIDDAVLLLDEKLKDKKGSISKKIKISNKKIARKKKS

Sequences:

>Translated_868_residues
MDHTLVIVESPTKAKTIRKFLPSNYEVLASMGHVRDLPKGAAEIPAAVKKEKWSRIGVNTTEDFEPLYIVPKDKKKVVKE
LKNALKGATQLLLATDEDREGESISWHLLQILKPKIPTKRMVFHEITKKAINKALDQTREIDMELVQAQETRRILDRLFG
YELSPLLWKKVAPRLSAGRVQSVSVRLLVRRERERRSFKKASYWGIKASLEKDNITFETRLFSLKGQRISNGSDFDEQTG
KLKRGNKSLIIREEKVNELLKDFSSEDWLVSKIEKKPSIRKPVPPFTTSTLQQEANRKLRLSARETMRCAQGLYERGFIT
YMRTDSVHLSEQATRAARECVSSMYGKEYLSNSPRQFNSTARNAQEAHEAIRPAGEVFKTPKETNLTGRDLSLYDLVWKR
TVASQMAEARLTMINAEISVGDGLFKSSGKSIDFAGFFRAYVEGSDDPSSSLEQQEIILPNLTTGTCLEVTDKEPTFHET
KPPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNTLAPTFTAFAVTALLEEHFPDLVDTTFTAKMESSLD
EISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYRQVDFEDLPCVVRIGSNGPWLEGIKIDESGNEIQAKGNLPM
DITPGDLDKKQVDQILSGPSDLGTDPKTGEKVFLRFGPYGPYVQLGNNDQDKAKPRRASLPKELKTDDLTLDEALVLLSL
PRLLGVHPEGGIVEADRGRFGPYIKWIKNENESENRSLKKEDDVFTVDLKRALEILAMPKMGRGGQEVLKDFGKPKEFKD
KIQILNGRYGVYLKCGKTNVSLAKDTDLEKISIDDAVLLLDEKLKDKKGSISKKIKISNKKIARKKKS
>Mature_868_residues
MDHTLVIVESPTKAKTIRKFLPSNYEVLASMGHVRDLPKGAAEIPAAVKKEKWSRIGVNTTEDFEPLYIVPKDKKKVVKE
LKNALKGATQLLLATDEDREGESISWHLLQILKPKIPTKRMVFHEITKKAINKALDQTREIDMELVQAQETRRILDRLFG
YELSPLLWKKVAPRLSAGRVQSVSVRLLVRRERERRSFKKASYWGIKASLEKDNITFETRLFSLKGQRISNGSDFDEQTG
KLKRGNKSLIIREEKVNELLKDFSSEDWLVSKIEKKPSIRKPVPPFTTSTLQQEANRKLRLSARETMRCAQGLYERGFIT
YMRTDSVHLSEQATRAARECVSSMYGKEYLSNSPRQFNSTARNAQEAHEAIRPAGEVFKTPKETNLTGRDLSLYDLVWKR
TVASQMAEARLTMINAEISVGDGLFKSSGKSIDFAGFFRAYVEGSDDPSSSLEQQEIILPNLTTGTCLEVTDKEPTFHET
KPPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNTLAPTFTAFAVTALLEEHFPDLVDTTFTAKMESSLD
EISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYRQVDFEDLPCVVRIGSNGPWLEGIKIDESGNEIQAKGNLPM
DITPGDLDKKQVDQILSGPSDLGTDPKTGEKVFLRFGPYGPYVQLGNNDQDKAKPRRASLPKELKTDDLTLDEALVLLSL
PRLLGVHPEGGIVEADRGRFGPYIKWIKNENESENRSLKKEDDVFTVDLKRALEILAMPKMGRGGQEVLKDFGKPKEFKD
KIQILNGRYGVYLKCGKTNVSLAKDTDLEKISIDDAVLLLDEKLKDKKGSISKKIKISNKKIARKKKS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=476, Percent_Identity=25.6302521008403, Blast_Score=120, Evalue=7e-27,
Organism=Homo sapiens, GI4507635, Length=613, Percent_Identity=23.3278955954323, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1787529, Length=596, Percent_Identity=40.4362416107383, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1788061, Length=554, Percent_Identity=23.826714801444, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32563869, Length=576, Percent_Identity=24.3055555555556, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=527, Percent_Identity=25.6166982922201, Blast_Score=117, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=520, Percent_Identity=23.2692307692308, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24585251, Length=470, Percent_Identity=27.6595744680851, Blast_Score=132, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640096, Length=523, Percent_Identity=21.9885277246654, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97601; Mature: 97601

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHTLVIVESPTKAKTIRKFLPSNYEVLASMGHVRDLPKGAAEIPAAVKKEKWSRIGVNT
CCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCC
TEDFEPLYIVPKDKKKVVKELKNALKGATQLLLATDEDREGESISWHLLQILKPKIPTKR
CCCCCCEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHCCCCCHHH
MVFHEITKKAINKALDQTREIDMELVQAQETRRILDRLFGYELSPLLWKKVAPRLSAGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
QSVSVRLLVRRERERRSFKKASYWGIKASLEKDNITFETRLFSLKGQRISNGSDFDEQTG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCHHHHC
KLKRGNKSLIIREEKVNELLKDFSSEDWLVSKIEKKPSIRKPVPPFTTSTLQQEANRKLR
HHHCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEE
LSARETMRCAQGLYERGFITYMRTDSVHLSEQATRAARECVSSMYGKEYLSNSPRQFNST
EHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARNAQEAHEAIRPAGEVFKTPKETNLTGRDLSLYDLVWKRTVASQMAEARLTMINAEISV
HHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
GDGLFKSSGKSIDFAGFFRAYVEGSDDPSSSLEQQEIILPNLTTGTCLEVTDKEPTFHET
CCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCEEEECCCCCCCCCC
KPPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNTLAPTFTAFAVTALLE
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
EHFPDLVDTTFTAKMESSLDEISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYR
HHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCEE
QVDFEDLPCVVRIGSNGPWLEGIKIDESGNEIQAKGNLPMDITPGDLDKKQVDQILSGPS
ECCCCCCCEEEEECCCCCEEEEEEECCCCCEEEECCCCCEECCCCCCCHHHHHHHHCCCH
DLGTDPKTGEKVFLRFGPYGPYVQLGNNDQDKAKPRRASLPKELKTDDLTLDEALVLLSL
HCCCCCCCCCEEEEEECCCCCEEEECCCCHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
PRLLGVHPEGGIVEADRGRFGPYIKWIKNENESENRSLKKEDDVFTVDLKRALEILAMPK
HHHHCCCCCCCEEECCCCCCCCHHHHHCCCCCCHHCCCCCCCCEEEEEHHHHHHHHHCCC
MGRGGQEVLKDFGKPKEFKDKIQILNGRYGVYLKCGKTNVSLAKDTDLEKISIDDAVLLL
CCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCEEECCCEEEEE
DEKLKDKKGSISKKIKISNKKIARKKKS
CHHHCCCCCCCCEEEEECCHHHHHCCCC
>Mature Secondary Structure
MDHTLVIVESPTKAKTIRKFLPSNYEVLASMGHVRDLPKGAAEIPAAVKKEKWSRIGVNT
CCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCC
TEDFEPLYIVPKDKKKVVKELKNALKGATQLLLATDEDREGESISWHLLQILKPKIPTKR
CCCCCCEEEECCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHCCCCCHHH
MVFHEITKKAINKALDQTREIDMELVQAQETRRILDRLFGYELSPLLWKKVAPRLSAGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
QSVSVRLLVRRERERRSFKKASYWGIKASLEKDNITFETRLFSLKGQRISNGSDFDEQTG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCHHHHC
KLKRGNKSLIIREEKVNELLKDFSSEDWLVSKIEKKPSIRKPVPPFTTSTLQQEANRKLR
HHHCCCCEEEEEHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCEEE
LSARETMRCAQGLYERGFITYMRTDSVHLSEQATRAARECVSSMYGKEYLSNSPRQFNST
EHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARNAQEAHEAIRPAGEVFKTPKETNLTGRDLSLYDLVWKRTVASQMAEARLTMINAEISV
HHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEC
GDGLFKSSGKSIDFAGFFRAYVEGSDDPSSSLEQQEIILPNLTTGTCLEVTDKEPTFHET
CCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCEEEECCCCCCCCCC
KPPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNTLAPTFTAFAVTALLE
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
EHFPDLVDTTFTAKMESSLDEISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYR
HHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCEE
QVDFEDLPCVVRIGSNGPWLEGIKIDESGNEIQAKGNLPMDITPGDLDKKQVDQILSGPS
ECCCCCCCEEEEECCCCCEEEEEEECCCCCEEEECCCCCEECCCCCCCHHHHHHHHCCCH
DLGTDPKTGEKVFLRFGPYGPYVQLGNNDQDKAKPRRASLPKELKTDDLTLDEALVLLSL
HCCCCCCCCCEEEEEECCCCCEEEECCCCHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
PRLLGVHPEGGIVEADRGRFGPYIKWIKNENESENRSLKKEDDVFTVDLKRALEILAMPK
HHHHCCCCCCCEEECCCCCCCCHHHHHCCCCCCHHCCCCCCCCEEEEEHHHHHHHHHCCC
MGRGGQEVLKDFGKPKEFKDKIQILNGRYGVYLKCGKTNVSLAKDTDLEKISIDDAVLLL
CCCCHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCCCCCEEECCCEEEEE
DEKLKDKKGSISKKIKISNKKIARKKKS
CHHHCCCCCCCCEEEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7659748 [H]