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The map label for this gene is birA [C]

Identifier: 78778817

GI number: 78778817

Start: 407194

End: 407943

Strand: Direct

Name: birA [C]

Synonym: PMT9312_0432

Alternate gene names: 78778817

Gene position: 407194-407943 (Clockwise)

Preceding gene: 78778815

Following gene: 78778820

Centisome position: 23.82

GC content: 29.2

Gene sequence:

>750_bases
GTGAAATTTATTGGACCTGCAGCTAAGACAGTTTTTTATTTAAAAAAAATTCAGGGTCAATATCCAACTTGGACACTTCA
TTACAAAATAAAATGTAAAAGTACCGAAAATGAGCTAACAAACTTGCTTGAATATTCTCAAATAAAGAGAAACCAGCCAA
TAGCGATAATAGCAAGAGAACAGTCCTCAGGTTTTGGCCAAAACTCAAAAACTTGGGTTTCTCCAAAAGGCGGGATTTGG
TTAAGTGCAGCTTATCCAATATTTTCAAAAGAATTTACAAGCCAAATATTTAATTTGTCTTTAGGAATTAAGATATGTGA
AATGCTTAGAGAAGAAAATATAAATGTTTTTTTGAAATGGCCAAATGATATTTTTGTTGGTTCAAAAAAGTTAATTGGAT
TTTTACCAAGGGTGATAACAAGAGGTAAGGAAATTATTTATGTAAGAATAGGTATTGGAATGAATGTTTTAAATCAAACT
CCATTAGAAGGTATTTCATTATCAAAAGTACTTAAAACTAAGAATATTAATCAACATTATTGGACAGCTAAAGTTCTTAA
AGCTTTTCATGATTCAATTGAATGTAATAACAAAAAAGAATATGTGATCAAATCGGCAAATAAGTACCTTACTAAAAGGT
TTTTACCTAGTGGTTTTTGTCCTCATACATGGAAAATTAAAGATATTGATTCGAATGGGAATTTAAGAATTCAAAATGAA
ACTCAACTTAAGGTAATTAGAAGGTTCTGA

Upstream 100 bases:

>100_bases
CTATTGGCAAATACTTTTTGTCCAATTTGTAATATTTACTTATTAAGACTAAAGTTAATCTAATCTTTAAAAAGATGCTC
GTTGTTTTAATAAAGAATTT

Downstream 100 bases:

>100_bases
ATTTATTTAACTTTTAATTCAACTATTCTTCCATCTTTAAATTTGGCTATTTTTTTTGCACGATTGGCTACTTCATCTTC
GTGCGTAACTAAAACTATTG

Product: putative biotin--acetyl-CoA-carboxylase ligase

Products: AMP; diphosphate; [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Alternate protein names: Biotin/Acetyl-CoA-Carboxylase Ligase; Biotin-(Acetyl-CoA Carboxylase) Ligase; Biotin-Protein Ligase; Biotin-(Acetyl-CoA-Carboxylase) Ligase; Biotin-[Acetyl-CoA-Carboxylase] Ligase; Biotin; Biotin-(Acetyl-CoA Carboxylase) Synthetase; Biotin Ligase; BirA Bifunctional Protein; Biotin Acetyl-CoA Carboxylase Ligase; Biotin Acetyl CoA Carboxylase Ligase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKFIGPAAKTVFYLKKIQGQYPTWTLHYKIKCKSTENELTNLLEYSQIKRNQPIAIIAREQSSGFGQNSKTWVSPKGGIW
LSAAYPIFSKEFTSQIFNLSLGIKICEMLREENINVFLKWPNDIFVGSKKLIGFLPRVITRGKEIIYVRIGIGMNVLNQT
PLEGISLSKVLKTKNINQHYWTAKVLKAFHDSIECNNKKEYVIKSANKYLTKRFLPSGFCPHTWKIKDIDSNGNLRIQNE
TQLKVIRRF

Sequences:

>Translated_249_residues
MKFIGPAAKTVFYLKKIQGQYPTWTLHYKIKCKSTENELTNLLEYSQIKRNQPIAIIAREQSSGFGQNSKTWVSPKGGIW
LSAAYPIFSKEFTSQIFNLSLGIKICEMLREENINVFLKWPNDIFVGSKKLIGFLPRVITRGKEIIYVRIGIGMNVLNQT
PLEGISLSKVLKTKNINQHYWTAKVLKAFHDSIECNNKKEYVIKSANKYLTKRFLPSGFCPHTWKIKDIDSNGNLRIQNE
TQLKVIRRF
>Mature_249_residues
MKFIGPAAKTVFYLKKIQGQYPTWTLHYKIKCKSTENELTNLLEYSQIKRNQPIAIIAREQSSGFGQNSKTWVSPKGGIW
LSAAYPIFSKEFTSQIFNLSLGIKICEMLREENINVFLKWPNDIFVGSKKLIGFLPRVITRGKEIIYVRIGIGMNVLNQT
PLEGISLSKVLKTKNINQHYWTAKVLKAFHDSIECNNKKEYVIKSANKYLTKRFLPSGFCPHTWKIKDIDSNGNLRIQNE
TQLKVIRRF

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.4.15

Molecular weight: Translated: 28751; Mature: 28751

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIGPAAKTVFYLKKIQGQYPTWTLHYKIKCKSTENELTNLLEYSQIKRNQPIAIIARE
CCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEC
QSSGFGQNSKTWVSPKGGIWLSAAYPIFSKEFTSQIFNLSLGIKICEMLREENINVFLKW
CCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PNDIFVGSKKLIGFLPRVITRGKEIIYVRIGIGMNVLNQTPLEGISLSKVLKTKNINQHY
CCCEEECCHHHHHHHHHHHHCCCEEEEEEEECCCCHHCCCCCCCCHHHHHHHHCCCCCHH
WTAKVLKAFHDSIECNNKKEYVIKSANKYLTKRFLPSGFCPHTWKIKDIDSNGNLRIQNE
HHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECC
TQLKVIRRF
HHHHHEECC
>Mature Secondary Structure
MKFIGPAAKTVFYLKKIQGQYPTWTLHYKIKCKSTENELTNLLEYSQIKRNQPIAIIARE
CCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEC
QSSGFGQNSKTWVSPKGGIWLSAAYPIFSKEFTSQIFNLSLGIKICEMLREENINVFLKW
CCCCCCCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PNDIFVGSKKLIGFLPRVITRGKEIIYVRIGIGMNVLNQTPLEGISLSKVLKTKNINQHY
CCCEEECCHHHHHHHHHHHHCCCEEEEEEEECCCCHHCCCCCCCCHHHHHHHHCCCCCHH
WTAKVLKAFHDSIECNNKKEYVIKSANKYLTKRFLPSGFCPHTWKIKDIDSNGNLRIQNE
HHHHHHHHHHHHCCCCCCHHEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECC
TQLKVIRRF
HHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; biotin; apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Specific reaction: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

General reaction: Amination; Carboxylic acid formation (carboxamide formation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA