Click here to switch to the map view.

The map label for this gene is mltB [H]

Identifier: 78484843

GI number: 78484843

Start: 555463

End: 556692

Strand: Reverse

Name: mltB [H]

Synonym: Tcr_0498

Alternate gene names: 78484843

Gene position: 556692-555463 (Counterclockwise)

Preceding gene: 78484847

Following gene: 78484828

Centisome position: 22.93

GC content: 44.31

Gene sequence:

>1230_bases
ATGACGCCAAAATTTTACCGACATTTTTTCATGCTTTTTACGGCCTTTAGTATTTTAATTGGCCCTAAAGCTTATGCCTA
TACCCCGCCGCCGCAGTCAGAATCGGATTTCCAAACCTGGCTGGTAGAATTTAAAAAAAGCGCGTATGAACAAGGTATTT
CAAAAACGACTCTTAAAAAGGCCTTTAAAGATGTTCACTTAAACCAACGCGTTCTGGAACTGGACCGTCGCCAACCTGAA
TTTACCCGGACATTTTGGCAATACTTTAATCGAGCCGTGACCGACTGGCGAATTCAAACAGGGCAAGAATTGTATGAAAA
ATACCGTCCACAGCTTAGAGAAGTAACTCGAAAATATGGTGTTCCTGGCCGATTCTTAATTGCTTTCTGGGGAATGGAAA
CCAATTTCGGCGGCTACACAGGAAATACGCCAATCATTGAATCTTTGGCAACCTTATCCTATGATCCACGCCGCTCTGAG
TTCTTTCAAAAGGAATTGATGGCCGCTTTAAAAATCATTCAAGAAGGCAACATTAAACCAGAGCAAATGAAAGGTTCCTG
GGCTGGCGCAATGGGACAATGTCAGTTTATGCCAAGCAACTATTTGAAATACGCCGTGGATGCCGATGACTCCGGTAAAC
GAGATTTATGGAACAGCTTGGATGATGTGTTTCATTCCATGGGCAATTTTTTGAACACATTAGGTTGGCAAAAACAAGAA
AACTGGGGACGCGAAGTGACGTTGCCAGAAGGGTTTGATTTAGCTTTAGCCGACGGTAAAACCGAACGCCCTCTTCAAGA
GTGGAAGGATATGGGACTGACTTTAGCCGATGGCCGTGAGCTGCCCGTAGAAGACATGAAAGCAAAATTACTGCTCCCTT
ACGATTACCGCGGACCGGTGTTTTTAGTATTCCATAATTTCGATGTCATTAAACGATGGAATCGATCGGACAGCTACGCT
CTAGCGGTTGGTCATTTAGCAGATCGTATTGTGGGAAGAGCTCCTTTAAGCAAAGAAGCGCCAGAAGATGATAAGTCTTT
GGATCGTCAGCAAATGATTGAAATGCAAGAACGACTGTCCATGGCGGGAATAGACGTTGGAAAAACGGATGGCATTGCGG
GAAATAAAACGCGTGCCGCGATTCGCCAATACCAAACTCAAAAAGGGTTGCCGGCCGATGGGTACCCCTCTTTTAGAATG
CTCCAAATTCTACGTCAAAACCAAAGTTAA

Upstream 100 bases:

>100_bases
GACGGCCATGATTTAGTCAATGTTTAGGACTTGCACTTCCAATTAAAACGGTCCAGACTGCAAACAAACACAACAACTAT
AAGCACATTTGAACATCATT

Downstream 100 bases:

>100_bases
CCCCTCAAAAGAGGCTTTACAACTGGTCATCATCAACGTATGATTTATTCATTAACCATTTGATAATACTTAATTAATGC
ACTGTTTCACCTCTAAGAGA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTPKFYRHFFMLFTAFSILIGPKAYAYTPPPQSESDFQTWLVEFKKSAYEQGISKTTLKKAFKDVHLNQRVLELDRRQPE
FTRTFWQYFNRAVTDWRIQTGQELYEKYRPQLREVTRKYGVPGRFLIAFWGMETNFGGYTGNTPIIESLATLSYDPRRSE
FFQKELMAALKIIQEGNIKPEQMKGSWAGAMGQCQFMPSNYLKYAVDADDSGKRDLWNSLDDVFHSMGNFLNTLGWQKQE
NWGREVTLPEGFDLALADGKTERPLQEWKDMGLTLADGRELPVEDMKAKLLLPYDYRGPVFLVFHNFDVIKRWNRSDSYA
LAVGHLADRIVGRAPLSKEAPEDDKSLDRQQMIEMQERLSMAGIDVGKTDGIAGNKTRAAIRQYQTQKGLPADGYPSFRM
LQILRQNQS

Sequences:

>Translated_409_residues
MTPKFYRHFFMLFTAFSILIGPKAYAYTPPPQSESDFQTWLVEFKKSAYEQGISKTTLKKAFKDVHLNQRVLELDRRQPE
FTRTFWQYFNRAVTDWRIQTGQELYEKYRPQLREVTRKYGVPGRFLIAFWGMETNFGGYTGNTPIIESLATLSYDPRRSE
FFQKELMAALKIIQEGNIKPEQMKGSWAGAMGQCQFMPSNYLKYAVDADDSGKRDLWNSLDDVFHSMGNFLNTLGWQKQE
NWGREVTLPEGFDLALADGKTERPLQEWKDMGLTLADGRELPVEDMKAKLLLPYDYRGPVFLVFHNFDVIKRWNRSDSYA
LAVGHLADRIVGRAPLSKEAPEDDKSLDRQQMIEMQERLSMAGIDVGKTDGIAGNKTRAAIRQYQTQKGLPADGYPSFRM
LQILRQNQS
>Mature_408_residues
TPKFYRHFFMLFTAFSILIGPKAYAYTPPPQSESDFQTWLVEFKKSAYEQGISKTTLKKAFKDVHLNQRVLELDRRQPEF
TRTFWQYFNRAVTDWRIQTGQELYEKYRPQLREVTRKYGVPGRFLIAFWGMETNFGGYTGNTPIIESLATLSYDPRRSEF
FQKELMAALKIIQEGNIKPEQMKGSWAGAMGQCQFMPSNYLKYAVDADDSGKRDLWNSLDDVFHSMGNFLNTLGWQKQEN
WGREVTLPEGFDLALADGKTERPLQEWKDMGLTLADGRELPVEDMKAKLLLPYDYRGPVFLVFHNFDVIKRWNRSDSYAL
AVGHLADRIVGRAPLSKEAPEDDKSLDRQQMIEMQERLSMAGIDVGKTDGIAGNKTRAAIRQYQTQKGLPADGYPSFRML
QILRQNQS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=254, Percent_Identity=33.0708661417323, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47312; Mature: 47181

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKFYRHFFMLFTAFSILIGPKAYAYTPPPQSESDFQTWLVEFKKSAYEQGISKTTLKK
CCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AFKDVHLNQRVLELDRRQPEFTRTFWQYFNRAVTDWRIQTGQELYEKYRPQLREVTRKYG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPGRFLIAFWGMETNFGGYTGNTPIIESLATLSYDPRRSEFFQKELMAALKIIQEGNIKP
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCH
EQMKGSWAGAMGQCQFMPSNYLKYAVDADDSGKRDLWNSLDDVFHSMGNFLNTLGWQKQE
HHHCCCCCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
NWGREVTLPEGFDLALADGKTERPLQEWKDMGLTLADGRELPVEDMKAKLLLPYDYRGPV
CCCCEEECCCCCCEEECCCCCCCHHHHHHHCCCEEECCCCCCHHHCCCEEECCCCCCCCE
FLVFHNFDVIKRWNRSDSYALAVGHLADRIVGRAPLSKEAPEDDKSLDRQQMIEMQERLS
EEEEECHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
MAGIDVGKTDGIAGNKTRAAIRQYQTQKGLPADGYPSFRMLQILRQNQS
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TPKFYRHFFMLFTAFSILIGPKAYAYTPPPQSESDFQTWLVEFKKSAYEQGISKTTLKK
CCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AFKDVHLNQRVLELDRRQPEFTRTFWQYFNRAVTDWRIQTGQELYEKYRPQLREVTRKYG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VPGRFLIAFWGMETNFGGYTGNTPIIESLATLSYDPRRSEFFQKELMAALKIIQEGNIKP
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCH
EQMKGSWAGAMGQCQFMPSNYLKYAVDADDSGKRDLWNSLDDVFHSMGNFLNTLGWQKQE
HHHCCCCCCCCCCEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
NWGREVTLPEGFDLALADGKTERPLQEWKDMGLTLADGRELPVEDMKAKLLLPYDYRGPV
CCCCEEECCCCCCEEECCCCCCCHHHHHHHCCCEEECCCCCCHHHCCCEEECCCCCCCCE
FLVFHNFDVIKRWNRSDSYALAVGHLADRIVGRAPLSKEAPEDDKSLDRQQMIEMQERLS
EEEEECHHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
MAGIDVGKTDGIAGNKTRAAIRQYQTQKGLPADGYPSFRMLQILRQNQS
HHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]