Click here to switch to the map view.

The map label for this gene is ispB [H]

Identifier: 78484685

GI number: 78484685

Start: 389179

End: 390144

Strand: Reverse

Name: ispB [H]

Synonym: Tcr_0340

Alternate gene names: 78484685

Gene position: 390144-389179 (Counterclockwise)

Preceding gene: 78484696

Following gene: 78484683

Centisome position: 16.07

GC content: 45.13

Gene sequence:

>966_bases
ATGACGCTTTCACAGATTCGAAAACTTATTCAGGATGACATTGAGGCGACTGACCAATTGATTCTGTCTAAACTGTCTTC
CGATGTTGTTCTTATTAATCAAATTGGCCACTACATTATCAACAGTGGTGGAAAGCGTTTACGCCCTTTACTGGTCTTAT
TGTCCGCTCACGCCTGTGACTACAAAGGAAAAGACCACTTAACGTTGGCGGCCGTGATTGAATTCATTCACACATCCACC
TTACTACATGACGATGTCGTCGATGAATCAGAAACTCGACGCGGCAATCAGACGGCCAATGAAGTCTGGGGAAATGCCGC
CAGTGTACTGGTCGGTGATTTCTTATACTCACGCTCATTCGAAATGATGGTGGAACCTGGAATTATGCGCGTCATGGAAG
TGATGTCTGAAACCACGAATGTGATTGCAGAAGGTGAAGTCCTCCAATTACTCAACTGCCATGACGCCGACACCACGAGT
CAGCGCTACATGGAAGTCATTCACCGAAAAACCGCCAAACTGTTTGAAGCCGCCACTTTATTAGGGCCTATCCTGGGTAA
AAAACCCGAACTAGAATCGGCTTTTTCATCCTATGGTCGCCACCTTGGTGCCGCCTTCCAACTCATCGATGATGCACTAG
ATTACACGGCCGATGCCGATGAACTTGGTAAAAACCTAGGAGACGATCTGGCCGAAGGGAAACCTACTCTGCCTTTAATC
TATGTACTTGAAAATGGAACCGAAAACGAAAAGTCATTGATTCGTAAAGCCATTGAAACCGAAGGCATTAAATTATTGAA
AGAAGTCACTTCCATCATTCAAACATCCGGTGCCATTCAATACACTCAAGAAGTTGCGACGCAAGAAGCTGAAAAAGCTA
AAGCCGCTCTCGCTCCGCTGAACGATTCTGAGATTAAACAAGCGTTATTATCTTTGGCAGACTTAGCGGTTCAAAGAAAC
CACTAA

Upstream 100 bases:

>100_bases
TTTTTTCTTGAAAAAAAACATTTACAGCTTTGCATCTATAGCGTTTCATACTAAACTTTAGCGTCTTACATTTACTAACC
AAGATAATTCCATTTTACTT

Downstream 100 bases:

>100_bases
CGCCCGTCCCCACTTTATTTAACTCTTGAACTCGGAATTCTTTGTTTCCCTTTAAAATGCTTATCAAGCGTTTGCTGTAG
CTGAGCTTCTTGCTTTTCCG

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTLSQIRKLIQDDIEATDQLILSKLSSDVVLINQIGHYIINSGGKRLRPLLVLLSAHACDYKGKDHLTLAAVIEFIHTST
LLHDDVVDESETRRGNQTANEVWGNAASVLVGDFLYSRSFEMMVEPGIMRVMEVMSETTNVIAEGEVLQLLNCHDADTTS
QRYMEVIHRKTAKLFEAATLLGPILGKKPELESAFSSYGRHLGAAFQLIDDALDYTADADELGKNLGDDLAEGKPTLPLI
YVLENGTENEKSLIRKAIETEGIKLLKEVTSIIQTSGAIQYTQEVATQEAEKAKAALAPLNDSEIKQALLSLADLAVQRN
H

Sequences:

>Translated_321_residues
MTLSQIRKLIQDDIEATDQLILSKLSSDVVLINQIGHYIINSGGKRLRPLLVLLSAHACDYKGKDHLTLAAVIEFIHTST
LLHDDVVDESETRRGNQTANEVWGNAASVLVGDFLYSRSFEMMVEPGIMRVMEVMSETTNVIAEGEVLQLLNCHDADTTS
QRYMEVIHRKTAKLFEAATLLGPILGKKPELESAFSSYGRHLGAAFQLIDDALDYTADADELGKNLGDDLAEGKPTLPLI
YVLENGTENEKSLIRKAIETEGIKLLKEVTSIIQTSGAIQYTQEVATQEAEKAKAALAPLNDSEIKQALLSLADLAVQRN
H
>Mature_320_residues
TLSQIRKLIQDDIEATDQLILSKLSSDVVLINQIGHYIINSGGKRLRPLLVLLSAHACDYKGKDHLTLAAVIEFIHTSTL
LHDDVVDESETRRGNQTANEVWGNAASVLVGDFLYSRSFEMMVEPGIMRVMEVMSETTNVIAEGEVLQLLNCHDADTTSQ
RYMEVIHRKTAKLFEAATLLGPILGKKPELESAFSSYGRHLGAAFQLIDDALDYTADADELGKNLGDDLAEGKPTLPLIY
VLENGTENEKSLIRKAIETEGIKLLKEVTSIIQTSGAIQYTQEVATQEAEKAKAALAPLNDSEIKQALLSLADLAVQRNH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=297, Percent_Identity=28.956228956229, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=54.6583850931677, Blast_Score=356, Evalue=1e-100,
Organism=Escherichia coli, GI1786623, Length=235, Percent_Identity=31.4893617021277, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=278, Percent_Identity=35.2517985611511, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319475, Length=221, Percent_Identity=32.1266968325792, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6325188, Length=313, Percent_Identity=23.0031948881789, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=27.8523489932886, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281365769, Length=276, Percent_Identity=24.2753623188406, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24660002, Length=276, Percent_Identity=24.2753623188406, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35271; Mature: 35139

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSQIRKLIQDDIEATDQLILSKLSSDVVLINQIGHYIINSGGKRLRPLLVLLSAHACD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
YKGKDHLTLAAVIEFIHTSTLLHDDVVDESETRRGNQTANEVWGNAASVLVGDFLYSRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCH
EMMVEPGIMRVMEVMSETTNVIAEGEVLQLLNCHDADTTSQRYMEVIHRKTAKLFEAATL
HHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGPILGKKPELESAFSSYGRHLGAAFQLIDDALDYTADADELGKNLGDDLAEGKPTLPLI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCEEE
YVLENGTENEKSLIRKAIETEGIKLLKEVTSIIQTSGAIQYTQEVATQEAEKAKAALAPL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
NDSEIKQALLSLADLAVQRNH
CHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLSQIRKLIQDDIEATDQLILSKLSSDVVLINQIGHYIINSGGKRLRPLLVLLSAHACD
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
YKGKDHLTLAAVIEFIHTSTLLHDDVVDESETRRGNQTANEVWGNAASVLVGDFLYSRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCH
EMMVEPGIMRVMEVMSETTNVIAEGEVLQLLNCHDADTTSQRYMEVIHRKTAKLFEAATL
HHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGPILGKKPELESAFSSYGRHLGAAFQLIDDALDYTADADELGKNLGDDLAEGKPTLPLI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCCEEE
YVLENGTENEKSLIRKAIETEGIKLLKEVTSIIQTSGAIQYTQEVATQEAEKAKAALAPL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
NDSEIKQALLSLADLAVQRNH
CHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]