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The map label for this gene is thiD [H]

Identifier: 78484488

GI number: 78484488

Start: 175676

End: 176521

Strand: Reverse

Name: thiD [H]

Synonym: Tcr_0143

Alternate gene names: 78484488

Gene position: 176521-175676 (Counterclockwise)

Preceding gene: 78484489

Following gene: 78484487

Centisome position: 7.27

GC content: 47.16

Gene sequence:

>846_bases
ATGGATAACCACACGCCCACGGTATTGAGCATTGCAGGATCTGACCCTTATGGCGGTGCCGGCATTCAGGCCGACTTGAA
AAGCATCCATGCTTTAGGAGGGTATGCGCTGACGTGCATTACCGCCCTAACAGCACAGAATTCTCAGGGGGTTGCACACG
TTGTCGCAACCTCGTCGGACATCTTAAACCATCAGCTGGATACCTTACTGCAGGACATCAAGGTGGACGCCGTCAAAATC
GGCATGCTCGCCAATGCCGAATTAATTAATGTGGTCGCTGACAAAATCACTCAATATTCGCTTAAAAATATTGTGTTAGA
TACCGTCCTCATAAGTTCCAGTGGTCACCCTTTGCTGGAAGAAAGCGCGATCGAGTGTTTAAAAAAGCGACTCTTTCCAA
AAGCCACCATCATTACTCCAAATTTACCCGAGATCAATCACCTCTTAGGCACACATTTTCATGGCATTGCACAGGAAATG
GATGCCATCAAGGAAGCTTTTTTGAAACTGGGTGCTCAAGCGGTTGTAGCAAAAGGGGGACACGGCTTAGAGACCGATCT
GGCGACCGATGTGTTACTTCAGCCAAATCATCCCCCTATGCCCTTTTCAAGCCCCCGCATTCAAACCTCTCATACACATG
GTACCGGATGCAGCTTTTCATCCGCCATTGCAACATTCCTTGCTCAGGGTAACGATTTAACTTCTGCCGTCCAACAGGCC
AAACAACAAATGCATCTATGGTTTGGTCAAAGTGAAAGCCTTCAATTTGATTATCAGTCCAATCTTACCCATCGTAAAGA
ACCCCTACATCACTTGAACTTGGGCACGGAACCGTCAAACAATTAA

Upstream 100 bases:

>100_bases
ATGATTAACGGGCTCAACCTGCCAGGCTGTACGACAATGGAAAGTATACACAAACTCATCCATACCTTTGATCAGGCTTA
CCAAACAAGAGCATTGTCCT

Downstream 100 bases:

>100_bases
TAAGGCACTTCGTTTCACTTAGTCTTTTTGCTAAGATTGACTCTCCTATTCTGAAAGGTGATGATCATGACTTCTCCTTC
CATCCAAAATATTAAAGCCG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MDNHTPTVLSIAGSDPYGGAGIQADLKSIHALGGYALTCITALTAQNSQGVAHVVATSSDILNHQLDTLLQDIKVDAVKI
GMLANAELINVVADKITQYSLKNIVLDTVLISSSGHPLLEESAIECLKKRLFPKATIITPNLPEINHLLGTHFHGIAQEM
DAIKEAFLKLGAQAVVAKGGHGLETDLATDVLLQPNHPPMPFSSPRIQTSHTHGTGCSFSSAIATFLAQGNDLTSAVQQA
KQQMHLWFGQSESLQFDYQSNLTHRKEPLHHLNLGTEPSNN

Sequences:

>Translated_281_residues
MDNHTPTVLSIAGSDPYGGAGIQADLKSIHALGGYALTCITALTAQNSQGVAHVVATSSDILNHQLDTLLQDIKVDAVKI
GMLANAELINVVADKITQYSLKNIVLDTVLISSSGHPLLEESAIECLKKRLFPKATIITPNLPEINHLLGTHFHGIAQEM
DAIKEAFLKLGAQAVVAKGGHGLETDLATDVLLQPNHPPMPFSSPRIQTSHTHGTGCSFSSAIATFLAQGNDLTSAVQQA
KQQMHLWFGQSESLQFDYQSNLTHRKEPLHHLNLGTEPSNN
>Mature_281_residues
MDNHTPTVLSIAGSDPYGGAGIQADLKSIHALGGYALTCITALTAQNSQGVAHVVATSSDILNHQLDTLLQDIKVDAVKI
GMLANAELINVVADKITQYSLKNIVLDTVLISSSGHPLLEESAIECLKKRLFPKATIITPNLPEINHLLGTHFHGIAQEM
DAIKEAFLKLGAQAVVAKGGHGLETDLATDVLLQPNHPPMPFSSPRIQTSHTHGTGCSFSSAIATFLAQGNDLTSAVQQA
KQQMHLWFGQSESLQFDYQSNLTHRKEPLHHLNLGTEPSNN

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=264, Percent_Identity=41.2878787878788, Blast_Score=210, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6324997, Length=246, Percent_Identity=39.0243902439024, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6325378, Length=242, Percent_Identity=34.7107438016529, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324517, Length=242, Percent_Identity=39.2561983471074, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30125; Mature: 30125

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNHTPTVLSIAGSDPYGGAGIQADLKSIHALGGYALTCITALTAQNSQGVAHVVATSSD
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCHH
ILNHQLDTLLQDIKVDAVKIGMLANAELINVVADKITQYSLKNIVLDTVLISSSGHPLLE
HHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
ESAIECLKKRLFPKATIITPNLPEINHLLGTHFHGIAQEMDAIKEAFLKLGAQAVVAKGG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCC
HGLETDLATDVLLQPNHPPMPFSSPRIQTSHTHGTGCSFSSAIATFLAQGNDLTSAVQQA
CCCCHHHHHHEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
KQQMHLWFGQSESLQFDYQSNLTHRKEPLHHLNLGTEPSNN
HHHHHHHCCCCCCEEEEHHCCCCHHHCCHHHCCCCCCCCCC
>Mature Secondary Structure
MDNHTPTVLSIAGSDPYGGAGIQADLKSIHALGGYALTCITALTAQNSQGVAHVVATSSD
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCHH
ILNHQLDTLLQDIKVDAVKIGMLANAELINVVADKITQYSLKNIVLDTVLISSSGHPLLE
HHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
ESAIECLKKRLFPKATIITPNLPEINHLLGTHFHGIAQEMDAIKEAFLKLGAQAVVAKGG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCC
HGLETDLATDVLLQPNHPPMPFSSPRIQTSHTHGTGCSFSSAIATFLAQGNDLTSAVQQA
CCCCHHHHHHEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
KQQMHLWFGQSESLQFDYQSNLTHRKEPLHHLNLGTEPSNN
HHHHHHHCCCCCCEEEEHHCCCCHHHCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]