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The map label for this gene is thiE [H]

Identifier: 78484489

GI number: 78484489

Start: 176514

End: 177170

Strand: Reverse

Name: thiE [H]

Synonym: Tcr_0144

Alternate gene names: 78484489

Gene position: 177170-176514 (Counterclockwise)

Preceding gene: 78484490

Following gene: 78484488

Centisome position: 7.3

GC content: 47.49

Gene sequence:

>657_bases
ATGCAATTCTTATCCTGCCATTCCCCTGAAAAACCACTGGGAATTTACCCGCTGGTGGACCGAGCCGATAAACTGCTTCC
TTTTTTTGACGCGGGCATCACCACCGCTCAAATTCGTATAAAAGATTTAGACGGTCAACCATTAGAGCAGGAAATGGCGC
AGGCGGACACTCTAGCAAAGTCGTATCAAGCCAGGCTCTTCATCAATGATTACTGGGAAATGGCGCTTCAACTCAACAGT
TATGGCGTACATTTAGGTCAAGAAGATGTCTTGAAGGCTGACTTACCGGCAATGCACCGAGCAGGGCTTCGACTGGGCGT
TAGCACGCATACGCCTGAAGAAATTGCTTCGGCGTTAACAATACAGCCCTCTTACATTGCTATCGGCCCCGTTTTCGAAA
CCCAAAGCAAGCAATTACCTTATCCAACCACCGGTTTAAATAATTTACACCACTGGGTGAAAACGTTGAATGTCCCAGTG
GTTGCCATTGGTGGTATTCAACGGCATAATCTGACTGACGTCATACTCGCTGGCGCGAACGGTGTCGCCATGATTAACGG
GCTCAACCTGCCAGGCTGTACGACAATGGAAAGTATACACAAACTCATCCATACCTTTGATCAGGCTTACCAAACAAGAG
CATTGTCCTATGGATAA

Upstream 100 bases:

>100_bases
ACGATGCAGGAAAGAGATTTGGCTTCGCCGTCAACCCCAGTGGTCGGCACGCCTTTTTGGCATCAACATTAACCAGGCCT
GGCCGTTTTAAGGAGCCATT

Downstream 100 bases:

>100_bases
CCACACGCCCACGGTATTGAGCATTGCAGGATCTGACCCTTATGGCGGTGCCGGCATTCAGGCCGACTTGAAAAGCATCC
ATGCTTTAGGAGGGTATGCG

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MQFLSCHSPEKPLGIYPLVDRADKLLPFFDAGITTAQIRIKDLDGQPLEQEMAQADTLAKSYQARLFINDYWEMALQLNS
YGVHLGQEDVLKADLPAMHRAGLRLGVSTHTPEEIASALTIQPSYIAIGPVFETQSKQLPYPTTGLNNLHHWVKTLNVPV
VAIGGIQRHNLTDVILAGANGVAMINGLNLPGCTTMESIHKLIHTFDQAYQTRALSYG

Sequences:

>Translated_218_residues
MQFLSCHSPEKPLGIYPLVDRADKLLPFFDAGITTAQIRIKDLDGQPLEQEMAQADTLAKSYQARLFINDYWEMALQLNS
YGVHLGQEDVLKADLPAMHRAGLRLGVSTHTPEEIASALTIQPSYIAIGPVFETQSKQLPYPTTGLNNLHHWVKTLNVPV
VAIGGIQRHNLTDVILAGANGVAMINGLNLPGCTTMESIHKLIHTFDQAYQTRALSYG
>Mature_218_residues
MQFLSCHSPEKPLGIYPLVDRADKLLPFFDAGITTAQIRIKDLDGQPLEQEMAQADTLAKSYQARLFINDYWEMALQLNS
YGVHLGQEDVLKADLPAMHRAGLRLGVSTHTPEEIASALTIQPSYIAIGPVFETQSKQLPYPTTGLNNLHHWVKTLNVPV
VAIGGIQRHNLTDVILAGANGVAMINGLNLPGCTTMESIHKLIHTFDQAYQTRALSYG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=177, Percent_Identity=46.8926553672316, Blast_Score=158, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=176, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24002; Mature: 24002

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLSCHSPEKPLGIYPLVDRADKLLPFFDAGITTAQIRIKDLDGQPLEQEMAQADTLAK
CCCCCCCCCCCCCCCCHHHHHHHHHCHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
SYQARLFINDYWEMALQLNSYGVHLGQEDVLKADLPAMHRAGLRLGVSTHTPEEIASALT
HHHEEEEEEHHHHHHEECCCCCEECCHHHHHHHCCCHHHHCCEEECCCCCCHHHHHHHEE
IQPSYIAIGPVFETQSKQLPYPTTGLNNLHHWVKTLNVPVVAIGGIQRHNLTDVILAGAN
CCCCEEEECCEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCC
GVAMINGLNLPGCTTMESIHKLIHTFDQAYQTRALSYG
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQFLSCHSPEKPLGIYPLVDRADKLLPFFDAGITTAQIRIKDLDGQPLEQEMAQADTLAK
CCCCCCCCCCCCCCCCHHHHHHHHHCHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
SYQARLFINDYWEMALQLNSYGVHLGQEDVLKADLPAMHRAGLRLGVSTHTPEEIASALT
HHHEEEEEEHHHHHHEECCCCCEECCHHHHHHHCCCHHHHCCEEECCCCCCHHHHHHHEE
IQPSYIAIGPVFETQSKQLPYPTTGLNNLHHWVKTLNVPVVAIGGIQRHNLTDVILAGAN
CCCCEEEECCEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCC
GVAMINGLNLPGCTTMESIHKLIHTFDQAYQTRALSYG
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA