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The map label for this gene is asnB [H]

Identifier: 78358716

GI number: 78358716

Start: 3637615

End: 3639513

Strand: Reverse

Name: asnB [H]

Synonym: Dde_3677

Alternate gene names: 78358716

Gene position: 3639513-3637615 (Counterclockwise)

Preceding gene: 78358717

Following gene: 78358715

Centisome position: 97.57

GC content: 61.77

Gene sequence:

>1899_bases
ATGTGCGGAATAGCAGGGTACATAGGCAGGCGCTGTCTTCCGGATGACGCCGTGCGGCGCTGCCATGCGGCCATGCGCCA
CCGCGGGCCGGACGGCAGCGGCACATTCCGGCGGTGTACCGCGGACGGCAGGCATGTGCTGCTTCTGCATGCACGGCTTT
CCATACTGGACCCCGACCCGCGTTCGGGTCAGCCTATGCAGCGCGGCGGCGGAGTGCTGGCATTCAACGGCGAGCTGTAC
AATTTTTCCGAAGTGCGTGAACGGCTTGAGCGGGGCGGGCTGGCTTTTGACACCACCGGCGATACCGAGGTGCTGTTGCG
GGCGCTGGATCCGCAGGGGCGGTCCCTGTTGTGCGGGGGCCACGCCGCAGAAGACCTGCTGCACGGCATGGCGGGAGCGC
TGGACGCATGCGAAGGCATGTGGGCCTTTGCCTACTTTGATGCTTCGCGCGGCACGCTGGTGCTGGGACGTGACCGTTTC
GGCGAAAAGCCGTTGTATTACCTGCGCGATGACGACGGCGGGCTGTATTTCGGCTCGGAGCCTAAACTGCTGGCTGCGCT
GACAGGGCGGCCCCTGCGGCCCGATACCGAGCATCTGCGCCGGTATCTGGTAAACGGCTACAAGTCACTGTACAAAACCC
GGCAGACGTTTTTTGCCGGTCTGCGTCAGGTGCCCGCCGGAACCATGCTGGCGGCGGATGCCGGCGGCCTGCGGGGACTG
TGCTACTGGGAGCGTCCTCATGCCGCGCACGACCCCGTGCAGGAAGACATGAGTTTTGCAGACGCTGTGCGCGGCACGCG
CGAGCGCCTCATCCGCTCCGTGGAGCTGCGTCTGAGAGCTGATGTGCCGCTGGCGTTCTGCATGAGTGGCGGAATTGATT
CCAATTCGCTTATCTGCATCGCCCGCAGGGTGTTCGGGTACGATGTGCACGGATTCACGGTCATGAACACCGACGCCCGC
TACGAAGAGCGTGAATGCGTGGAGCATGTGGTCCGCGAGCTCGGACTGCGGCATACCTCGGTGTCTCCTTCCGTCGCGGG
GTTTCTGGACGGGCTGCGGACTCTGGTGCGTGCCCACGATGCGCCGGTGCTGACCATTTCATATTATGTGCACTGGCTGC
TCATGCAGCGTATCCGGCAGCAGGGATATGCCATATCCATCAGCGGTACGGCCGCCGATGAACTGTTCACCGGCTACTAC
GACCATCATAATCTGTATCTGGCCGCCATGTACGGTTCGGGCGCCTTGTACGACAAAGCGCTGGAAAACTGGCGCCGCCA
TGTGGCACCCGTGGTGCGCAACCCGTATCTGCAGGACCCGGAGGTGTTTGTGCACGCCCCGCAGGAACGCGGCCACATAT
ACCTGCATAACGACAGATTCGCCGGGGCGTTGCGTCAGCCTTTCGACGAGCCTTTTACGGAACATGCCTACCACGCCGTG
CCCCTGCGCAACAGAATGCTGAACGAACTGTTCCACGAAGCGGTTCCAGTCATTCTGCATGAGGACGACCTCAACGCCAT
GTACCATTCGGTGGAAAACCGTTCCCCTTTTCTGGACAGAGATCTGTTTGATTTTTCGCTGCGCATACCTGTGCGGCATC
TTGTGCGCGACGGAATGGCAAAGGCTGTGCTGCGCGAAGCCATGCGCGGCATTGTGCCCGATATGATTCTGGATAACCGC
CGCAAGGTGGGGTTCAATGCGCCGGTGCTTGATTTTCTGGATGTGCGGGACAGCGCGGTCCGGGCGCAGGTCTTGGCGGA
CAGTCCCGTATGGGACTTTGTGCGGCGTGATGCCGTGGCGCAGCTGCTGGACGGCCGGTCTCTGGGCAACAGCGAAAGCA
AGTTTTTGTTTAACATCCTGTGCTGCAAGATGTTTCTTGAAGACTGCGCCGATGCGTGA

Upstream 100 bases:

>100_bases
TGGGGCACATAGTGCTGGCAGGTGACGCGCTGCATGTGGTGTCTTCTGCGCGCGATCTGGCTGTTGCCGAAACGTTGCTG
CGTCTGGAGCGTGAACGTAT

Downstream 100 bases:

>100_bases
GGCGTTTATTTCAATAACTGTCCGGTTGCTTTTCGTAAGTTTTGCGTACGCTTTTCGAAAGAGGTGTATTTTATGAGAAT
GATACTGGAAATGCTTTTCG

Product: asparagine synthase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MCGIAGYIGRRCLPDDAVRRCHAAMRHRGPDGSGTFRRCTADGRHVLLLHARLSILDPDPRSGQPMQRGGGVLAFNGELY
NFSEVRERLERGGLAFDTTGDTEVLLRALDPQGRSLLCGGHAAEDLLHGMAGALDACEGMWAFAYFDASRGTLVLGRDRF
GEKPLYYLRDDDGGLYFGSEPKLLAALTGRPLRPDTEHLRRYLVNGYKSLYKTRQTFFAGLRQVPAGTMLAADAGGLRGL
CYWERPHAAHDPVQEDMSFADAVRGTRERLIRSVELRLRADVPLAFCMSGGIDSNSLICIARRVFGYDVHGFTVMNTDAR
YEERECVEHVVRELGLRHTSVSPSVAGFLDGLRTLVRAHDAPVLTISYYVHWLLMQRIRQQGYAISISGTAADELFTGYY
DHHNLYLAAMYGSGALYDKALENWRRHVAPVVRNPYLQDPEVFVHAPQERGHIYLHNDRFAGALRQPFDEPFTEHAYHAV
PLRNRMLNELFHEAVPVILHEDDLNAMYHSVENRSPFLDRDLFDFSLRIPVRHLVRDGMAKAVLREAMRGIVPDMILDNR
RKVGFNAPVLDFLDVRDSAVRAQVLADSPVWDFVRRDAVAQLLDGRSLGNSESKFLFNILCCKMFLEDCADA

Sequences:

>Translated_632_residues
MCGIAGYIGRRCLPDDAVRRCHAAMRHRGPDGSGTFRRCTADGRHVLLLHARLSILDPDPRSGQPMQRGGGVLAFNGELY
NFSEVRERLERGGLAFDTTGDTEVLLRALDPQGRSLLCGGHAAEDLLHGMAGALDACEGMWAFAYFDASRGTLVLGRDRF
GEKPLYYLRDDDGGLYFGSEPKLLAALTGRPLRPDTEHLRRYLVNGYKSLYKTRQTFFAGLRQVPAGTMLAADAGGLRGL
CYWERPHAAHDPVQEDMSFADAVRGTRERLIRSVELRLRADVPLAFCMSGGIDSNSLICIARRVFGYDVHGFTVMNTDAR
YEERECVEHVVRELGLRHTSVSPSVAGFLDGLRTLVRAHDAPVLTISYYVHWLLMQRIRQQGYAISISGTAADELFTGYY
DHHNLYLAAMYGSGALYDKALENWRRHVAPVVRNPYLQDPEVFVHAPQERGHIYLHNDRFAGALRQPFDEPFTEHAYHAV
PLRNRMLNELFHEAVPVILHEDDLNAMYHSVENRSPFLDRDLFDFSLRIPVRHLVRDGMAKAVLREAMRGIVPDMILDNR
RKVGFNAPVLDFLDVRDSAVRAQVLADSPVWDFVRRDAVAQLLDGRSLGNSESKFLFNILCCKMFLEDCADA
>Mature_632_residues
MCGIAGYIGRRCLPDDAVRRCHAAMRHRGPDGSGTFRRCTADGRHVLLLHARLSILDPDPRSGQPMQRGGGVLAFNGELY
NFSEVRERLERGGLAFDTTGDTEVLLRALDPQGRSLLCGGHAAEDLLHGMAGALDACEGMWAFAYFDASRGTLVLGRDRF
GEKPLYYLRDDDGGLYFGSEPKLLAALTGRPLRPDTEHLRRYLVNGYKSLYKTRQTFFAGLRQVPAGTMLAADAGGLRGL
CYWERPHAAHDPVQEDMSFADAVRGTRERLIRSVELRLRADVPLAFCMSGGIDSNSLICIARRVFGYDVHGFTVMNTDAR
YEERECVEHVVRELGLRHTSVSPSVAGFLDGLRTLVRAHDAPVLTISYYVHWLLMQRIRQQGYAISISGTAADELFTGYY
DHHNLYLAAMYGSGALYDKALENWRRHVAPVVRNPYLQDPEVFVHAPQERGHIYLHNDRFAGALRQPFDEPFTEHAYHAV
PLRNRMLNELFHEAVPVILHEDDLNAMYHSVENRSPFLDRDLFDFSLRIPVRHLVRDGMAKAVLREAMRGIVPDMILDNR
RKVGFNAPVLDFLDVRDSAVRAQVLADSPVWDFVRRDAVAQLLDGRSLGNSESKFLFNILCCKMFLEDCADA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=429, Percent_Identity=27.972027972028, Blast_Score=122, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45553209, Length=388, Percent_Identity=25, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71122; Mature: 71122

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGYIGRRCLPDDAVRRCHAAMRHRGPDGSGTFRRCTADGRHVLLLHARLSILDPDP
CCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEHEEECCCC
RSGQPMQRGGGVLAFNGELYNFSEVRERLERGGLAFDTTGDTEVLLRALDPQGRSLLCGG
CCCCCHHHCCCEEEECCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCEEEECC
HAAEDLLHGMAGALDACEGMWAFAYFDASRGTLVLGRDRFGEKPLYYLRDDDGGLYFGSE
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCEEECCC
PKLLAALTGRPLRPDTEHLRRYLVNGYKSLYKTRQTFFAGLRQVPAGTMLAADAGGLRGL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCE
CYWERPHAAHDPVQEDMSFADAVRGTRERLIRSVELRLRADVPLAFCMSGGIDSNSLICI
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCCCEEE
ARRVFGYDVHGFTVMNTDARYEERECVEHVVRELGLRHTSVSPSVAGFLDGLRTLVRAHD
EHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
APVLTISYYVHWLLMQRIRQQGYAISISGTAADELFTGYYDHHNLYLAAMYGSGALYDKA
CCEEEHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCEECCCCEEEEEEECCCHHHHHH
LENWRRHVAPVVRNPYLQDPEVFVHAPQERGHIYLHNDRFAGALRQPFDEPFTEHAYHAV
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHCCCCCCHHHHCEEEC
PLRNRMLNELFHEAVPVILHEDDLNAMYHSVENRSPFLDRDLFDFSLRIPVRHLVRDGMA
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCHHHHEEECCHHHHHHHHHH
KAVLREAMRGIVPDMILDNRRKVGFNAPVLDFLDVRDSAVRAQVLADSPVWDFVRRDAVA
HHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHH
QLLDGRSLGNSESKFLFNILCCKMFLEDCADA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGYIGRRCLPDDAVRRCHAAMRHRGPDGSGTFRRCTADGRHVLLLHARLSILDPDP
CCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEHEEECCCC
RSGQPMQRGGGVLAFNGELYNFSEVRERLERGGLAFDTTGDTEVLLRALDPQGRSLLCGG
CCCCCHHHCCCEEEECCCCCCHHHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCEEEECC
HAAEDLLHGMAGALDACEGMWAFAYFDASRGTLVLGRDRFGEKPLYYLRDDDGGLYFGSE
HHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCEEECCC
PKLLAALTGRPLRPDTEHLRRYLVNGYKSLYKTRQTFFAGLRQVPAGTMLAADAGGLRGL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCE
CYWERPHAAHDPVQEDMSFADAVRGTRERLIRSVELRLRADVPLAFCMSGGIDSNSLICI
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHCCCCCCCCCEEE
ARRVFGYDVHGFTVMNTDARYEERECVEHVVRELGLRHTSVSPSVAGFLDGLRTLVRAHD
EHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
APVLTISYYVHWLLMQRIRQQGYAISISGTAADELFTGYYDHHNLYLAAMYGSGALYDKA
CCEEEHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCEECCCCEEEEEEECCCHHHHHH
LENWRRHVAPVVRNPYLQDPEVFVHAPQERGHIYLHNDRFAGALRQPFDEPFTEHAYHAV
HHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHCCCCCCHHHHCEEEC
PLRNRMLNELFHEAVPVILHEDDLNAMYHSVENRSPFLDRDLFDFSLRIPVRHLVRDGMA
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCHHHHEEECCHHHHHHHHHH
KAVLREAMRGIVPDMILDNRRKVGFNAPVLDFLDVRDSAVRAQVLADSPVWDFVRRDAVA
HHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHH
QLLDGRSLGNSESKFLFNILCCKMFLEDCADA
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]