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The map label for this gene is lacA [C]

Identifier: 78358715

GI number: 78358715

Start: 3636916

End: 3637542

Strand: Reverse

Name: lacA [C]

Synonym: Dde_3676

Alternate gene names: 78358715

Gene position: 3637542-3636916 (Counterclockwise)

Preceding gene: 78358716

Following gene: 78358714

Centisome position: 97.52

GC content: 60.61

Gene sequence:

>627_bases
ATGAGAATGATACTGGAAATGCTTTTCGGCGACCGTCTGTGGCGAGTCTGGAGTCTGCTGATAGCCTGGGCGCTGCGCTG
TTACGGCGTGCAGGTGGGCCGCGGTTTTTTCATCAAGGGGCGCATCCTGCTCAAACTGCGCGGCAGACGCGACCGCATAA
TCATCGGAGACAATGTTTCCGTGTGGGGTGATCTGGATCTGCGCAACCGTGAAAACGGCACCATAATTCTGCGGGACGGT
GTGGTGGTTGACGGGCCGTGCCGGCTGGTGGCGGCGCGGGACGCCGTGGTGGACATAGGAGAGCAGTGCGCTCTTACGCC
GTATCTGCTGCTTAATGCCGGAGCCGATGTCCGCATAGGCCGCGGTACGATCATCGGTCCCCGTGTGAGCATCAACTCCA
GCGAGCATGTCTACGACCGCAGCGTGCCCGTCAGGCAGTCCGGTTACACGCACGAACCGGTGATCATCGGCAGTGACTGC
TGGCTGGCGGCCAACGTGTCCGTAACAAAGGGCGTGACCATAGCCGATGGCAGCGTTATCGGTGCCAATGCCGTGGTGAC
CGCAGATACCCTGCCGTATTCGGTCAACGTGGGTGTGCCTGCCAGAAAAATCTGCGACAGGCCCTGA

Upstream 100 bases:

>100_bases
GTTTCTTGAAGACTGCGCCGATGCGTGAGGCGTTTATTTCAATAACTGTCCGGTTGCTTTTCGTAAGTTTTGCGTACGCT
TTTCGAAAGAGGTGTATTTT

Downstream 100 bases:

>100_bases
AGAATACCGGAGCCACAGATATGGGCGGCACGCTGCATCTCATAGACTCCTGCGCTGCATCGAAAGCGTTTCTGGCGGCA
TACGGCGCACAGGCCCGTCA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MRMILEMLFGDRLWRVWSLLIAWALRCYGVQVGRGFFIKGRILLKLRGRRDRIIIGDNVSVWGDLDLRNRENGTIILRDG
VVVDGPCRLVAARDAVVDIGEQCALTPYLLLNAGADVRIGRGTIIGPRVSINSSEHVYDRSVPVRQSGYTHEPVIIGSDC
WLAANVSVTKGVTIADGSVIGANAVVTADTLPYSVNVGVPARKICDRP

Sequences:

>Translated_208_residues
MRMILEMLFGDRLWRVWSLLIAWALRCYGVQVGRGFFIKGRILLKLRGRRDRIIIGDNVSVWGDLDLRNRENGTIILRDG
VVVDGPCRLVAARDAVVDIGEQCALTPYLLLNAGADVRIGRGTIIGPRVSINSSEHVYDRSVPVRQSGYTHEPVIIGSDC
WLAANVSVTKGVTIADGSVIGANAVVTADTLPYSVNVGVPARKICDRP
>Mature_208_residues
MRMILEMLFGDRLWRVWSLLIAWALRCYGVQVGRGFFIKGRILLKLRGRRDRIIIGDNVSVWGDLDLRNRENGTIILRDG
VVVDGPCRLVAARDAVVDIGEQCALTPYLLLNAGADVRIGRGTIIGPRVSINSSEHVYDRSVPVRQSGYTHEPVIIGSDC
WLAANVSVTKGVTIADGSVIGANAVVTADTLPYSVNVGVPARKICDRP

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=139, Percent_Identity=28.7769784172662, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22751; Mature: 22751

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMILEMLFGDRLWRVWSLLIAWALRCYGVQVGRGFFIKGRILLKLRGRRDRIIIGDNVS
CHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEECCEEEEEEEEEEEEECCCCEEEECCCCE
VWGDLDLRNRENGTIILRDGVVVDGPCRLVAARDAVVDIGEQCALTPYLLLNAGADVRIG
EEEEEECCCCCCCEEEEECCEEECCCEEEEEECCHHHCCCCCCCCCEEEEEECCCCEEEC
RGTIIGPRVSINSSEHVYDRSVPVRQSGYTHEPVIIGSDCWLAANVSVTKGVTIADGSVI
CCEEECCEEEECCCCCEEECCCCCCCCCCCCCCEEECCCCEEEECCEEECCEEEECCCEE
GANAVVTADTLPYSVNVGVPARKICDRP
CCCEEEEECCCCEEEECCCCHHHHCCCC
>Mature Secondary Structure
MRMILEMLFGDRLWRVWSLLIAWALRCYGVQVGRGFFIKGRILLKLRGRRDRIIIGDNVS
CHHHHHHHHCHHHHHHHHHHHHHHHHHHEEEECCEEEEEEEEEEEEECCCCEEEECCCCE
VWGDLDLRNRENGTIILRDGVVVDGPCRLVAARDAVVDIGEQCALTPYLLLNAGADVRIG
EEEEEECCCCCCCEEEEECCEEECCCEEEEEECCHHHCCCCCCCCCEEEEEECCCCEEEC
RGTIIGPRVSINSSEHVYDRSVPVRQSGYTHEPVIIGSDCWLAANVSVTKGVTIADGSVI
CCEEECCEEEECCCCCEEECCCCCCCCCCCCCCEEECCCCEEEECCEEECCEEEECCCEE
GANAVVTADTLPYSVNVGVPARKICDRP
CCCEEEEECCCCEEEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]