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The map label for this gene is fumB [H]

Identifier: 78358677

GI number: 78358677

Start: 3602102

End: 3602647

Strand: Reverse

Name: fumB [H]

Synonym: Dde_3638

Alternate gene names: 78358677

Gene position: 3602647-3602102 (Counterclockwise)

Preceding gene: 78358678

Following gene: 78358676

Centisome position: 96.58

GC content: 63.92

Gene sequence:

>546_bases
ATGGCTACCTGGCATCTTACCACACCACTCAGGTCTGAGGATGTGCTGCAACTGCGCGCCGGTGATGTCGTGCTGCTTTC
GGGCGATATATATACAGCCCGCGATGCGGCGCACAAACGCATCATGGAACAGCTGGACAGCGGCGGCACCCCGCCTTTTC
CGCCGGAAGGAGCGGTCGTGTACTATGTGGGACCGGCACCCGCGCCGCCGGGACGCCCCATCGGGTCGGCTGGGCCCACC
ACAAGCGGCCGGATGGACAGCTACACTCCCCGCATGCTTGCACTGGGGGTGCGTGCCACCATAGGCAAGGGAAAGCGCTG
CGACGCGGTGCGCAACGCCATGTGTGAGCATGGTGCTGTGTATCTGGGAGCCACGGGCGGCGCCGGTGCGCTGCTGTCAC
AGTGTGTTCTGGCCGCCGAGGTTGTGGCCTATGAAGAGCTGGGCCCGGAAGCCGTGCGCAGGCTGAAGGTGCGTGATTTT
CCGCTGCTGGTGATCAACGATGCCTGCGGCGGCGAGCTGTATGTAACCCCCAAACTTGATCTGTGA

Upstream 100 bases:

>100_bases
TGGCTGTGAAAATTGCCCTGCATCCCTGCCATATTGCCAGCCTGCCTGTGGCGGTGAATGTGCAGTGCCATTCGGCGCGG
CACAGAGAGGTGATTCTCTG

Downstream 100 bases:

>100_bases
CCACTCAGGAGGCTGACAATGGCTCTTTTCACAAAAGAAGAAGCACTGAAATATCATGAGGCTCCGCGCCCCGGTAAGCT
GGAAGTCGTTCCCGCAAAGC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MATWHLTTPLRSEDVLQLRAGDVVLLSGDIYTARDAAHKRIMEQLDSGGTPPFPPEGAVVYYVGPAPAPPGRPIGSAGPT
TSGRMDSYTPRMLALGVRATIGKGKRCDAVRNAMCEHGAVYLGATGGAGALLSQCVLAAEVVAYEELGPEAVRRLKVRDF
PLLVINDACGGELYVTPKLDL

Sequences:

>Translated_181_residues
MATWHLTTPLRSEDVLQLRAGDVVLLSGDIYTARDAAHKRIMEQLDSGGTPPFPPEGAVVYYVGPAPAPPGRPIGSAGPT
TSGRMDSYTPRMLALGVRATIGKGKRCDAVRNAMCEHGAVYLGATGGAGALLSQCVLAAEVVAYEELGPEAVRRLKVRDF
PLLVINDACGGELYVTPKLDL
>Mature_180_residues
ATWHLTTPLRSEDVLQLRAGDVVLLSGDIYTARDAAHKRIMEQLDSGGTPPFPPEGAVVYYVGPAPAPPGRPIGSAGPTT
SGRMDSYTPRMLALGVRATIGKGKRCDAVRNAMCEHGAVYLGATGGAGALLSQCVLAAEVVAYEELGPEAVRRLKVRDFP
LLVINDACGGELYVTPKLDL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=156, Percent_Identity=39.7435897435897, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1790564, Length=151, Percent_Identity=41.0596026490066, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1789443, Length=178, Percent_Identity=32.0224719101124, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 19073; Mature: 18942

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATWHLTTPLRSEDVLQLRAGDVVLLSGDIYTARDAAHKRIMEQLDSGGTPPFPPEGAVV
CCEEEECCCCCCCCEEEEECCCEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YYVGPAPAPPGRPIGSAGPTTSGRMDSYTPRMLALGVRATIGKGKRCDAVRNAMCEHGAV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEHHHCCCCCCCHHHHHHHHHHCCCE
YLGATGGAGALLSQCVLAAEVVAYEELGPEAVRRLKVRDFPLLVINDACGGELYVTPKLD
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCC
L
C
>Mature Secondary Structure 
ATWHLTTPLRSEDVLQLRAGDVVLLSGDIYTARDAAHKRIMEQLDSGGTPPFPPEGAVV
CEEEECCCCCCCCEEEEECCCEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YYVGPAPAPPGRPIGSAGPTTSGRMDSYTPRMLALGVRATIGKGKRCDAVRNAMCEHGAV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEHHHCCCCCCCHHHHHHHHHHCCCE
YLGATGGAGALLSQCVLAAEVVAYEELGPEAVRRLKVRDFPLLVINDACGGELYVTPKLD
EEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]