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The map label for this gene is fdhB [H]

Identifier: 78358553

GI number: 78358553

Start: 3474956

End: 3475684

Strand: Direct

Name: fdhB [H]

Synonym: Dde_3514

Alternate gene names: 78358553

Gene position: 3474956-3475684 (Clockwise)

Preceding gene: 78358552

Following gene: 78358554

Centisome position: 93.16

GC content: 60.22

Gene sequence:

>729_bases
ATGTCAAAAGCCATATTCATAGACACCACCCGCTGCACGGCGTGCCGCGGCTGCCAGCTTGCCTGCAAGGAATGGTACGA
GCTGCCCGCCAACAAAACGCGCCAGACAGGCAGCCACCAGAATCCGCCGGACCTCAACCCCAACAACTTCAAACTGGTCC
GTTTTCATGAACACATGATAGACGACCGCGTGGTCTGGTACTTCTTTCCCGACCAGTGCAGGCACTGCGTCAACCCGCCG
TGCGTGGACATCGCCAACATGATTGTGGACGGCGCACTGGTGCACGATGAAGAAACCGGCGCGGTGCTGACCACCGAAAA
ATCCAGCCTGCTTTCGGCCGAAGACTTTCAGGCCGTGCGCGACGCATGTCCCTATGACATTCCCCGCCGCGACGAGCAGA
CAGGACAGGTCAAAAAATGCGTCATGTGCTTTGACCGCATCAAGGCCGGCATGCAGCCTGCCTGCGTCAAGGTGTGCCCC
ACGGGCACCATGAACTTCGGAGAGCGCGAAGAGATGCTCAAGCTGGCCAGAACCCGGCTTGAAAAAGTCCGCAAGGAACA
CCCCCGCGCCATGCTGGCAGACCCCGATGACGTGAATGTCATCTTTCTGCTGGCAGACGACCCCGAACGGTACCACGAAT
ACAGCGTGGCTCAGGCCGAGCCCGTCAGCCGCAAGCGGCTGTTTGCCTCGCTGCGGCGTCCCGTGCGGGCCATGACGGAA
AACAGCTAG

Upstream 100 bases:

>100_bases
CTGCGTCAATATACGCAAGGCCGACACCCTGACCGAAATCGACCGATAGCCATACGGCGGACGGCCGCTTCATGTGGCCG
CCCCGCCAAGGAGTCATGAC

Downstream 100 bases:

>100_bases
GACCGAACAACAGCCCGGAAGGCCGCTTACCCGGCCTTCCGGCTTTACCGGAGCACGAATGCCACAATCACGCGAATCAG
TCAGTGCCACTCTGAACGCC

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: FDH subunit beta; Formate dehydrogenase small subunit [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSKAIFIDTTRCTACRGCQLACKEWYELPANKTRQTGSHQNPPDLNPNNFKLVRFHEHMIDDRVVWYFFPDQCRHCVNPP
CVDIANMIVDGALVHDEETGAVLTTEKSSLLSAEDFQAVRDACPYDIPRRDEQTGQVKKCVMCFDRIKAGMQPACVKVCP
TGTMNFGEREEMLKLARTRLEKVRKEHPRAMLADPDDVNVIFLLADDPERYHEYSVAQAEPVSRKRLFASLRRPVRAMTE
NS

Sequences:

>Translated_242_residues
MSKAIFIDTTRCTACRGCQLACKEWYELPANKTRQTGSHQNPPDLNPNNFKLVRFHEHMIDDRVVWYFFPDQCRHCVNPP
CVDIANMIVDGALVHDEETGAVLTTEKSSLLSAEDFQAVRDACPYDIPRRDEQTGQVKKCVMCFDRIKAGMQPACVKVCP
TGTMNFGEREEMLKLARTRLEKVRKEHPRAMLADPDDVNVIFLLADDPERYHEYSVAQAEPVSRKRLFASLRRPVRAMTE
NS
>Mature_241_residues
SKAIFIDTTRCTACRGCQLACKEWYELPANKTRQTGSHQNPPDLNPNNFKLVRFHEHMIDDRVVWYFFPDQCRHCVNPPC
VDIANMIVDGALVHDEETGAVLTTEKSSLLSAEDFQAVRDACPYDIPRRDEQTGQVKKCVMCFDRIKAGMQPACVKVCPT
GTMNFGEREEMLKLARTRLEKVRKEHPRAMLADPDDVNVIFLLADDPERYHEYSVAQAEPVSRKRLFASLRRPVRAMTEN
S

Specific function: Beta chain of the formate dehydrogenase (FDH) catalyze the reversible two-electron oxidation of formate to carbon dioxide. FDH loses activity in the presence of air, but this activity can be restored. This chain is an electron transfer unit [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=215, Percent_Identity=32.5581395348837, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1790326, Length=215, Percent_Identity=33.4883720930233, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787122, Length=171, Percent_Identity=29.8245614035088, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787872, Length=195, Percent_Identity=29.7435897435897, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789370, Length=209, Percent_Identity=28.7081339712919, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI226510944, Length=169, Percent_Identity=27.2189349112426, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 27693; Mature: 27562

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAIFIDTTRCTACRGCQLACKEWYELPANKTRQTGSHQNPPDLNPNNFKLVRFHEHMI
CCCEEEEECCCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHH
DDRVVWYFFPDQCRHCVNPPCVDIANMIVDGALVHDEETGAVLTTEKSSLLSAEDFQAVR
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHCCEEECCCCCEEEEECHHHHCCHHHHHHHH
DACPYDIPRRDEQTGQVKKCVMCFDRIKAGMQPACVKVCPTGTMNFGEREEMLKLARTRL
HCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
EKVRKEHPRAMLADPDDVNVIFLLADDPERYHEYSVAQAEPVSRKRLFASLRRPVRAMTE
HHHHHHCCCEEECCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
NS
CC
>Mature Secondary Structure 
SKAIFIDTTRCTACRGCQLACKEWYELPANKTRQTGSHQNPPDLNPNNFKLVRFHEHMI
CCEEEEECCCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHH
DDRVVWYFFPDQCRHCVNPPCVDIANMIVDGALVHDEETGAVLTTEKSSLLSAEDFQAVR
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHCCEEECCCCCEEEEECHHHHCCHHHHHHHH
DACPYDIPRRDEQTGQVKKCVMCFDRIKAGMQPACVKVCPTGTMNFGEREEMLKLARTRL
HCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
EKVRKEHPRAMLADPDDVNVIFLLADDPERYHEYSVAQAEPVSRKRLFASLRRPVRAMTE
HHHHHHCCCEEECCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
NS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA