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The map label for this gene is zraS [H]

Identifier: 78358504

GI number: 78358504

Start: 3425558

End: 3427027

Strand: Direct

Name: zraS [H]

Synonym: Dde_3465

Alternate gene names: 78358504

Gene position: 3425558-3427027 (Clockwise)

Preceding gene: 78358503

Following gene: 78358509

Centisome position: 91.83

GC content: 59.8

Gene sequence:

>1470_bases
ATGCAAACCGCACCGGCACAACAGGCCACGGTACTTACGGTGGAAGATGACCCTGTCATCCGCGAAACCATAGCAGCCTG
GCTGGAGGATTCCGGCCTGACCGTGCTGCAGGCCGAAGACGGCAGCAAAGGTCTGGACGTTTTCCGCAGGCACACACCAG
ACCTTGTACTGCTGGATCTCGGCATTCCCGGCATGAGCGGTGAAAGCCTGCTCAAGCTCATGGTGCAGGAACAGCCCGAC
ACGCCGGTCATCATCGTTTCCGGCACGGCGGAAATAGACGCCGCCATCAGCGCCTTCAAGAACGGCGCATGGGATTTCAT
CACCAAACCCGTGCCCAATATGGAACTGCTGGAAAAAACCGTGCGGAACTGTCTGGAACGCAAATCACTGCGCGAACGGC
TGGACGCCGCAGAGACCCGCTATTTCCAGTTCATCCAGCATCTGCCCGTGACGGTGTTTTCCATGGACGGCAGCATGCAG
GTCACATGGCTCAATGCCGCATGCCCCAGAGTGCTGGGCTGGCCCGTGGCAGAAGCGCTGGGGACGGAAAACTGGTTCAT
GGATAACGTGCACCCGCCGGACAGGCCCGCACTGCAACAGGCACTGGAAGCCGCGCTGCAGCGCGGGAACGACATTTTCT
GCGAATTTTCATTCCGCCACCGCCACGGCTACCTTATCCGCCTGCAGGCGCGCTCCACATCCGTATCTGCTCCGGACGCT
GCGCGCGCGGGCAGAATCGAAGGCGTTCTGCTTGATATCACCGACCGCGATTTTCTGGATAAGGTGCTTGTGCAGCGCGA
AAAACTGAACACGCTGGGAGCCATGGCCGATGAAGTGGCCCATGAATTCCGCAACCCGCTGTTTGCGCTGGGCGGTTTTG
CACGCAGACTGCATACGCGCATGCCCGACATCATGGAGGCCGGCATCATTCTGGAAGAAGCAAAACGCATGGAAACCATG
CTGGACAGGCTAAAAGAGTACACCAGCCCTGTGGACGTCTCGCCGGAACCCACCAATGTCAACACATCCGTCAATTTTGC
GCTGGACAGGCTGGAACCTCAGATAGCCAGACGCCGGGCGGCCGTTTCCAGCAATCTGGAAGCCGGCATGCCGGAAATAC
AGTCCGATCCCGACATTCTGCTGCAGGTGCTCATGAATCTGGTGACCAACCTGCTGGATGCCATGCCTGAAGGCGAAACG
CTGCGGCTGATCACCTCACAAAGTGCCGGAACACAGGAAGTGACGCTCATGGCACCGGTGCAGGGCACGCCCATGGCCGA
CCCCGAACAGCTGCTGCTGCCCTTTGACGTTTCCGGACCGCAAAGAAACCTTGCGGTAAGTTACCGGCTGGTAAAAACCA
TCGGCGGGCTGCTGTCCGTCACCCGCAGCCACAACGTGCATACGCTTACTCTTTCGCTGCCCGCCGGACACGACAGCCGC
GTACCCGGCACGGGTGCCGGTGCTTCCTGA

Upstream 100 bases:

>100_bases
GCTCGCGCTCCGTCGTAAACTGGGCAACCTGCATCCGCAGGACTTTGCACCATGCACAGTTTACGGCATCAACAGCGCGG
AACACGCGCGGGAATACGGC

Downstream 100 bases:

>100_bases
CGTGCTGCCGCCGCATAAAAAAAACGCCGCAGGACCTGCCCCCGGCGTTCTGAGCGTTACGCACCCTTTCCGGTATGCGC
CTATACCCCTGCACGGGGGT

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MQTAPAQQATVLTVEDDPVIRETIAAWLEDSGLTVLQAEDGSKGLDVFRRHTPDLVLLDLGIPGMSGESLLKLMVQEQPD
TPVIIVSGTAEIDAAISAFKNGAWDFITKPVPNMELLEKTVRNCLERKSLRERLDAAETRYFQFIQHLPVTVFSMDGSMQ
VTWLNAACPRVLGWPVAEALGTENWFMDNVHPPDRPALQQALEAALQRGNDIFCEFSFRHRHGYLIRLQARSTSVSAPDA
ARAGRIEGVLLDITDRDFLDKVLVQREKLNTLGAMADEVAHEFRNPLFALGGFARRLHTRMPDIMEAGIILEEAKRMETM
LDRLKEYTSPVDVSPEPTNVNTSVNFALDRLEPQIARRRAAVSSNLEAGMPEIQSDPDILLQVLMNLVTNLLDAMPEGET
LRLITSQSAGTQEVTLMAPVQGTPMADPEQLLLPFDVSGPQRNLAVSYRLVKTIGGLLSVTRSHNVHTLTLSLPAGHDSR
VPGTGAGAS

Sequences:

>Translated_489_residues
MQTAPAQQATVLTVEDDPVIRETIAAWLEDSGLTVLQAEDGSKGLDVFRRHTPDLVLLDLGIPGMSGESLLKLMVQEQPD
TPVIIVSGTAEIDAAISAFKNGAWDFITKPVPNMELLEKTVRNCLERKSLRERLDAAETRYFQFIQHLPVTVFSMDGSMQ
VTWLNAACPRVLGWPVAEALGTENWFMDNVHPPDRPALQQALEAALQRGNDIFCEFSFRHRHGYLIRLQARSTSVSAPDA
ARAGRIEGVLLDITDRDFLDKVLVQREKLNTLGAMADEVAHEFRNPLFALGGFARRLHTRMPDIMEAGIILEEAKRMETM
LDRLKEYTSPVDVSPEPTNVNTSVNFALDRLEPQIARRRAAVSSNLEAGMPEIQSDPDILLQVLMNLVTNLLDAMPEGET
LRLITSQSAGTQEVTLMAPVQGTPMADPEQLLLPFDVSGPQRNLAVSYRLVKTIGGLLSVTRSHNVHTLTLSLPAGHDSR
VPGTGAGAS
>Mature_489_residues
MQTAPAQQATVLTVEDDPVIRETIAAWLEDSGLTVLQAEDGSKGLDVFRRHTPDLVLLDLGIPGMSGESLLKLMVQEQPD
TPVIIVSGTAEIDAAISAFKNGAWDFITKPVPNMELLEKTVRNCLERKSLRERLDAAETRYFQFIQHLPVTVFSMDGSMQ
VTWLNAACPRVLGWPVAEALGTENWFMDNVHPPDRPALQQALEAALQRGNDIFCEFSFRHRHGYLIRLQARSTSVSAPDA
ARAGRIEGVLLDITDRDFLDKVLVQREKLNTLGAMADEVAHEFRNPLFALGGFARRLHTRMPDIMEAGIILEEAKRMETM
LDRLKEYTSPVDVSPEPTNVNTSVNFALDRLEPQIARRRAAVSSNLEAGMPEIQSDPDILLQVLMNLVTNLLDAMPEGET
LRLITSQSAGTQEVTLMAPVQGTPMADPEQLLLPFDVSGPQRNLAVSYRLVKTIGGLLSVTRSHNVHTLTLSLPAGHDSR
VPGTGAGAS

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=28.2608695652174, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=35.9375, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787487, Length=141, Percent_Identity=31.2056737588652, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788549, Length=334, Percent_Identity=24.5508982035928, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788550, Length=124, Percent_Identity=29.8387096774194, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788905, Length=121, Percent_Identity=34.7107438016529, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=33.0578512396694, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=120, Percent_Identity=35.8333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=32.2314049586777, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53776; Mature: 53776

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTAPAQQATVLTVEDDPVIRETIAAWLEDSGLTVLQAEDGSKGLDVFRRHTPDLVLLDL
CCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEEEEC
GIPGMSGESLLKLMVQEQPDTPVIIVSGTAEIDAAISAFKNGAWDFITKPVPNMELLEKT
CCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHH
VRNCLERKSLRERLDAAETRYFQFIQHLPVTVFSMDGSMQVTWLNAACPRVLGWPVAEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCHHHCCHHHHHH
GTENWFMDNVHPPDRPALQQALEAALQRGNDIFCEFSFRHRHGYLIRLQARSTSVSAPDA
CCCCCEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCC
ARAGRIEGVLLDITDRDFLDKVLVQREKLNTLGAMADEVAHEFRNPLFALGGFARRLHTR
HHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
MPDIMEAGIILEEAKRMETMLDRLKEYTSPVDVSPEPTNVNTSVNFALDRLEPQIARRRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHH
AVSSNLEAGMPEIQSDPDILLQVLMNLVTNLLDAMPEGETLRLITSQSAGTQEVTLMAPV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECC
QGTPMADPEQLLLPFDVSGPQRNLAVSYRLVKTIGGLLSVTRSHNVHTLTLSLPAGHDSR
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
VPGTGAGAS
CCCCCCCCC
>Mature Secondary Structure
MQTAPAQQATVLTVEDDPVIRETIAAWLEDSGLTVLQAEDGSKGLDVFRRHTPDLVLLDL
CCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEEEEC
GIPGMSGESLLKLMVQEQPDTPVIIVSGTAEIDAAISAFKNGAWDFITKPVPNMELLEKT
CCCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHH
VRNCLERKSLRERLDAAETRYFQFIQHLPVTVFSMDGSMQVTWLNAACPRVLGWPVAEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECCCCCHHHCCHHHHHH
GTENWFMDNVHPPDRPALQQALEAALQRGNDIFCEFSFRHRHGYLIRLQARSTSVSAPDA
CCCCCEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCC
ARAGRIEGVLLDITDRDFLDKVLVQREKLNTLGAMADEVAHEFRNPLFALGGFARRLHTR
HHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
MPDIMEAGIILEEAKRMETMLDRLKEYTSPVDVSPEPTNVNTSVNFALDRLEPQIARRRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHH
AVSSNLEAGMPEIQSDPDILLQVLMNLVTNLLDAMPEGETLRLITSQSAGTQEVTLMAPV
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECC
QGTPMADPEQLLLPFDVSGPQRNLAVSYRLVKTIGGLLSVTRSHNVHTLTLSLPAGHDSR
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCC
VPGTGAGAS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]