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The map label for this gene is hemB [H]

Identifier: 78357618

GI number: 78357618

Start: 2592436

End: 2593431

Strand: Direct

Name: hemB [H]

Synonym: Dde_2576

Alternate gene names: 78357618

Gene position: 2592436-2593431 (Clockwise)

Preceding gene: 78357617

Following gene: 78357619

Centisome position: 69.5

GC content: 59.24

Gene sequence:

>996_bases
GTGTCTGATTTTTTCCGGGGCAGAAGGTTGCGCCGCAACGCCCCCATGAGGGAACTTGTACGCGAGCATGACATTTCCGC
CCGTCATCTCGTCATGCCCTATTTTGTGGTGGAAACCGACGACGCCGGCTTCCGCAAGCCTATCACCTCCATGCCCGGCA
TTTTTCAGCTGTCGCTTGCCGAGCTGGAAAAAGACGTGGCCGAAGCCGTGGCACTGGGCATGCGTTCCGTTATTCTTTTC
GGCATTCCGGCGGCAAAAGATTACAAAGGCTCGGAAGCATACAACCACGACGGCATTGTCCAGAAAGCCATACGCCGGCT
GAAAGAGCGCTTTCCGGATCTTCTGGTAATCACCGATGTGTGTCTGTGCGAATATACCAGCCACGGTCACTGCGGACTGC
TGCGGCAGGGCGACACCACCGGTGAAGTGCACAATGACCCTACCCTGACCCTGCTGGCCAAGGCTGCGGTTTCGCACGCC
GAGGCAGGAGCCGACATCGTGGCCCCATCGGACATGATGGACGGGCGTGTGCTGGCCATCCGCGAAGCGCTGGATGCCAA
CGGCTTTTCACATATTCCCGTCATGTCCTACGCCGTCAAATACGCCAGCGCCTTTTACGGCCCCTTCCGCGAGGCGGCCG
AATCCACCCCCCAGCACGGCGACCGCAAGACCTATCAGATGGACCCGGCCAACAGCCGCGAGGCCATGCGCGAAGCCGCG
GCAGACGTGGCGGAGGGAGCAGACTTCCTCATTGTCAAACCTGCCGGTCCTTACATGGACATCATCCGCATGGTACGGGA
CAGCTTTGACCTGCCCGTTGCCGCCTATCAGGTCAGCGGCGAGTATTCCATGATCAAGGCTGCCGCCATCAACGACTGGA
TTGACGGCGACAAAGTGGCCATGGAGTCCCTTACGGGCCTGCGCCGCGCCGGTGCGGATATCATCATCAGTTATCTGACT
GTGGAAATGCTGCGCAAAAAGCTTGTTTCCGCATAA

Upstream 100 bases:

>100_bases
CTCCGGCGTGCCAGAACGGGCAGGCCGGAGCGTTTTTTACTGCTGCCTGCAAGTGCAGCAACAGCAAGCCAATTGCAACA
GAACCAAAGGAGCAGACACG

Downstream 100 bases:

>100_bases
AACGCAATGCTCTTCCGGCGCGCCGTCCCTGCGGGGCGTACGCGCCGGAATTTTTCAGCCGACCTGTGCAGCCCTGCACA
TGCCGTACAGGTACAAGGAC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSDFFRGRRLRRNAPMRELVREHDISARHLVMPYFVVETDDAGFRKPITSMPGIFQLSLAELEKDVAEAVALGMRSVILF
GIPAAKDYKGSEAYNHDGIVQKAIRRLKERFPDLLVITDVCLCEYTSHGHCGLLRQGDTTGEVHNDPTLTLLAKAAVSHA
EAGADIVAPSDMMDGRVLAIREALDANGFSHIPVMSYAVKYASAFYGPFREAAESTPQHGDRKTYQMDPANSREAMREAA
ADVAEGADFLIVKPAGPYMDIIRMVRDSFDLPVAAYQVSGEYSMIKAAAINDWIDGDKVAMESLTGLRRAGADIIISYLT
VEMLRKKLVSA

Sequences:

>Translated_331_residues
MSDFFRGRRLRRNAPMRELVREHDISARHLVMPYFVVETDDAGFRKPITSMPGIFQLSLAELEKDVAEAVALGMRSVILF
GIPAAKDYKGSEAYNHDGIVQKAIRRLKERFPDLLVITDVCLCEYTSHGHCGLLRQGDTTGEVHNDPTLTLLAKAAVSHA
EAGADIVAPSDMMDGRVLAIREALDANGFSHIPVMSYAVKYASAFYGPFREAAESTPQHGDRKTYQMDPANSREAMREAA
ADVAEGADFLIVKPAGPYMDIIRMVRDSFDLPVAAYQVSGEYSMIKAAAINDWIDGDKVAMESLTGLRRAGADIIISYLT
VEMLRKKLVSA
>Mature_330_residues
SDFFRGRRLRRNAPMRELVREHDISARHLVMPYFVVETDDAGFRKPITSMPGIFQLSLAELEKDVAEAVALGMRSVILFG
IPAAKDYKGSEAYNHDGIVQKAIRRLKERFPDLLVITDVCLCEYTSHGHCGLLRQGDTTGEVHNDPTLTLLAKAAVSHAE
AGADIVAPSDMMDGRVLAIREALDANGFSHIPVMSYAVKYASAFYGPFREAAESTPQHGDRKTYQMDPANSREAMREAAA
DVAEGADFLIVKPAGPYMDIIRMVRDSFDLPVAAYQVSGEYSMIKAAAINDWIDGDKVAMESLTGLRRAGADIIISYLTV
EMLRKKLVSA

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=305, Percent_Identity=46.5573770491803, Blast_Score=267, Evalue=8e-72,
Organism=Escherichia coli, GI87081728, Length=330, Percent_Identity=48.7878787878788, Blast_Score=301, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=44.2675159235669, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI21358291, Length=317, Percent_Identity=46.3722397476341, Blast_Score=260, Evalue=7e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36475; Mature: 36344

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFFRGRRLRRNAPMRELVREHDISARHLVMPYFVVETDDAGFRKPITSMPGIFQLSLA
CCCHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCHHCCCCHHHHHHH
ELEKDVAEAVALGMRSVILFGIPAAKDYKGSEAYNHDGIVQKAIRRLKERFPDLLVITDV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHH
CLCEYTSHGHCGLLRQGDTTGEVHNDPTLTLLAKAAVSHAEAGADIVAPSDMMDGRVLAI
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHH
REALDANGFSHIPVMSYAVKYASAFYGPFREAAESTPQHGDRKTYQMDPANSREAMREAA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHH
ADVAEGADFLIVKPAGPYMDIIRMVRDSFDLPVAAYQVSGEYSMIKAAAINDWIDGDKVA
HHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHH
MESLTGLRRAGADIIISYLTVEMLRKKLVSA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDFFRGRRLRRNAPMRELVREHDISARHLVMPYFVVETDDAGFRKPITSMPGIFQLSLA
CCHHHHHHHHCCCCHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCHHCCCCHHHHHHH
ELEKDVAEAVALGMRSVILFGIPAAKDYKGSEAYNHDGIVQKAIRRLKERFPDLLVITDV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHH
CLCEYTSHGHCGLLRQGDTTGEVHNDPTLTLLAKAAVSHAEAGADIVAPSDMMDGRVLAI
HHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHH
REALDANGFSHIPVMSYAVKYASAFYGPFREAAESTPQHGDRKTYQMDPANSREAMREAA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHH
ADVAEGADFLIVKPAGPYMDIIRMVRDSFDLPVAAYQVSGEYSMIKAAAINDWIDGDKVA
HHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHH
MESLTGLRRAGADIIISYLTVEMLRKKLVSA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]