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The map label for this gene is pqqE [H]

Identifier: 78357617

GI number: 78357617

Start: 2591140

End: 2592324

Strand: Direct

Name: pqqE [H]

Synonym: Dde_2575

Alternate gene names: 78357617

Gene position: 2591140-2592324 (Clockwise)

Preceding gene: 78357615

Following gene: 78357618

Centisome position: 69.46

GC content: 55.95

Gene sequence:

>1185_bases
ATGATAGGCATTTCAAAGCTGTACTGTGGACAGGTTGAACCCTCCGACGCGCTGCGCTACGGACGAGAATCGGGCAAAAT
GCCCTCGCACCTGCTTCAGTTCTCCAAAGACAAAAAGCCCGTGGTTGTGTGGAACATGACCCGCCGCTGCAACCTGAAGT
GTGTTCACTGCTACGCTCAGGCCGTGGACCCCGAAGGCAACGACGAAATCAGCACCGAACAGGGCAAAGAGATCATCCGC
GATCTGGCCCAGTACGGCGCACCGGTCATGCTGTTTTCCGGCGGCGAGCCGCTGGTGCGCAAAGACCTTGTGGAGCTGGC
CAAATTTGCCACCGAACAGGGCATGAGAGCGGTTATATCCACCAACGGCACGCTGATTACCAAAGAAAAAGCCCGCGAGC
TGAAGGAAGTGGGACTTTCGTACGTGGGGATTTCCCTTGACGGTATGGAAGAGGTGCACAACAAATTCCGCGGTGTGCCC
AACAGCTTTAAAAAAGCGCTCGAAGGCATTGAAAACTGTAAGGCCGAAGGGCTGAAAGTGGGCCTGCGCTTTACTATCAA
CAAGCGTAACGCACCGGAAGTTCCCAAAATATTTGACCTCATCAAAGAACTGGAAATACCCAGAATCTGCTTTTACCATC
TGGTATACTCCGGCCGCGGCAGCGAACTGATCAAGGAAGATCTTGATCATGCCGAAACCCGTGCCATGGTTGACCTTATC
ATGGACCGCACCCGCCAGCTCTTTGAAGAAGGCCACCCTAAAGAAGTGCTGACCGTGGACAACCACGCCGACGGCCCCTA
TGTGTGGATGCGCATGCTCAAGGAAGACCCCAAGCGTGCCGCTGAAGTATTCGAGCTGCTGCAGTACAACGAAGGCAACA
ACTCCGGCCGCGGCATCGGCTGCATCAGCTGGGACGGCACGGTGCATCCCGACCAGTTCTGGCGCAACAAGGTTTTCGGC
AACGTGCTGGAACGTCCTTTCTCGGAAATTTGGGACGACCCCGAAATCGAGCTGCTGGCAAAGCTGAAAGACAAAAAACA
GCATGTCACCGGCCGCTGCGCCCAGTGCCGCTTCCTCAATATCTGCGGCGGCAACTTCCGCGCCCGTGCCGAATCCTACT
ACGGCGATGTGTGGGCTCCCGATCCGGCCTGCTTCCTCACCGACGAGGAAATCGGCATCAAATAG

Upstream 100 bases:

>100_bases
TTCGCAGGGTATTCCTGCTGTGTCGGTGGCTGCACGCAGCGGCCAAACGATGCGTGTTTACGCGGAATGATTCGGGCTTT
CATGCATACCAGGAGACATA

Downstream 100 bases:

>100_bases
CCAACTTTCCGCTCCGGCGTGCCAGAACGGGCAGGCCGGAGCGTTTTTTACTGCTGCCTGCAAGTGCAGCAACAGCAAGC
CAATTGCAACAGAACCAAAG

Product: Elongator protein 3/MiaB/NifB

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MIGISKLYCGQVEPSDALRYGRESGKMPSHLLQFSKDKKPVVVWNMTRRCNLKCVHCYAQAVDPEGNDEISTEQGKEIIR
DLAQYGAPVMLFSGGEPLVRKDLVELAKFATEQGMRAVISTNGTLITKEKARELKEVGLSYVGISLDGMEEVHNKFRGVP
NSFKKALEGIENCKAEGLKVGLRFTINKRNAPEVPKIFDLIKELEIPRICFYHLVYSGRGSELIKEDLDHAETRAMVDLI
MDRTRQLFEEGHPKEVLTVDNHADGPYVWMRMLKEDPKRAAEVFELLQYNEGNNSGRGIGCISWDGTVHPDQFWRNKVFG
NVLERPFSEIWDDPEIELLAKLKDKKQHVTGRCAQCRFLNICGGNFRARAESYYGDVWAPDPACFLTDEEIGIK

Sequences:

>Translated_394_residues
MIGISKLYCGQVEPSDALRYGRESGKMPSHLLQFSKDKKPVVVWNMTRRCNLKCVHCYAQAVDPEGNDEISTEQGKEIIR
DLAQYGAPVMLFSGGEPLVRKDLVELAKFATEQGMRAVISTNGTLITKEKARELKEVGLSYVGISLDGMEEVHNKFRGVP
NSFKKALEGIENCKAEGLKVGLRFTINKRNAPEVPKIFDLIKELEIPRICFYHLVYSGRGSELIKEDLDHAETRAMVDLI
MDRTRQLFEEGHPKEVLTVDNHADGPYVWMRMLKEDPKRAAEVFELLQYNEGNNSGRGIGCISWDGTVHPDQFWRNKVFG
NVLERPFSEIWDDPEIELLAKLKDKKQHVTGRCAQCRFLNICGGNFRARAESYYGDVWAPDPACFLTDEEIGIK
>Mature_394_residues
MIGISKLYCGQVEPSDALRYGRESGKMPSHLLQFSKDKKPVVVWNMTRRCNLKCVHCYAQAVDPEGNDEISTEQGKEIIR
DLAQYGAPVMLFSGGEPLVRKDLVELAKFATEQGMRAVISTNGTLITKEKARELKEVGLSYVGISLDGMEEVHNKFRGVP
NSFKKALEGIENCKAEGLKVGLRFTINKRNAPEVPKIFDLIKELEIPRICFYHLVYSGRGSELIKEDLDHAETRAMVDLI
MDRTRQLFEEGHPKEVLTVDNHADGPYVWMRMLKEDPKRAAEVFELLQYNEGNNSGRGIGCISWDGTVHPDQFWRNKVFG
NVLERPFSEIWDDPEIELLAKLKDKKQHVTGRCAQCRFLNICGGNFRARAESYYGDVWAPDPACFLTDEEIGIK

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32566329, Length=204, Percent_Identity=26.4705882352941, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 44677; Mature: 44677

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGISKLYCGQVEPSDALRYGRESGKMPSHLLQFSKDKKPVVVWNMTRRCNLKCVHCYAQ
CCCCCCHHCCCCCHHHHHHHCHHCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEHH
AVDPEGNDEISTEQGKEIIRDLAQYGAPVMLFSGGEPLVRKDLVELAKFATEQGMRAVIS
HCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEE
TNGTLITKEKARELKEVGLSYVGISLDGMEEVHNKFRGVPNSFKKALEGIENCKAEGLKV
CCCCEEEHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
GLRFTINKRNAPEVPKIFDLIKELEIPRICFYHLVYSGRGSELIKEDLDHAETRAMVDLI
EEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MDRTRQLFEEGHPKEVLTVDNHADGPYVWMRMLKEDPKRAAEVFELLQYNEGNNSGRGIG
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEE
CISWDGTVHPDQFWRNKVFGNVLERPFSEIWDDPEIELLAKLKDKKQHVTGRCAQCRFLN
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCEEEEE
ICGGNFRARAESYYGDVWAPDPACFLTDEEIGIK
ECCCCCHHHHHHHCCCCCCCCCCEEEECHHCCCC
>Mature Secondary Structure
MIGISKLYCGQVEPSDALRYGRESGKMPSHLLQFSKDKKPVVVWNMTRRCNLKCVHCYAQ
CCCCCCHHCCCCCHHHHHHHCHHCCCCHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEHH
AVDPEGNDEISTEQGKEIIRDLAQYGAPVMLFSGGEPLVRKDLVELAKFATEQGMRAVIS
HCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEE
TNGTLITKEKARELKEVGLSYVGISLDGMEEVHNKFRGVPNSFKKALEGIENCKAEGLKV
CCCCEEEHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEE
GLRFTINKRNAPEVPKIFDLIKELEIPRICFYHLVYSGRGSELIKEDLDHAETRAMVDLI
EEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
MDRTRQLFEEGHPKEVLTVDNHADGPYVWMRMLKEDPKRAAEVFELLQYNEGNNSGRGIG
HHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEE
CISWDGTVHPDQFWRNKVFGNVLERPFSEIWDDPEIELLAKLKDKKQHVTGRCAQCRFLN
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCEEEEE
ICGGNFRARAESYYGDVWAPDPACFLTDEEIGIK
ECCCCCHHHHHHHCCCCCCCCCCEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]