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The map label for this gene is ispG

Identifier: 78357250

GI number: 78357250

Start: 2247741

End: 2248820

Strand: Reverse

Name: ispG

Synonym: Dde_2207

Alternate gene names: 78357250

Gene position: 2248820-2247741 (Counterclockwise)

Preceding gene: 78357257

Following gene: 78357249

Centisome position: 60.29

GC content: 56.2

Gene sequence:

>1080_bases
ATGACCATTGCACGCAGAAAATCGCGCGAAGTAACCATTGGAAACGTGCGTATCGGCGGAGATAATCCCGTAGTTGTCCA
GAGCATGACCAACACCGATACGCGTGACGTCGAGCAGACCGTGGAACAGATCAACAGCCTTGCCGAAGCCGGCTGCGAGA
TTGTCCGCGTGGCTGTGCTTAATGATGATGCTGCATGGGCTATCAGGCCGATCAAAGATCAGGTCACGGTGCCGTTGGTG
GCGGACATTCATTTTGACCATCGTCTCGCTGTTTCCGCTCTGGAGGCAGGCGTGGACGCCCTGCGCATCAATCCGGGCAA
CATCGGCGGTCAGAAAAACGTGGACAAGGTGGTGGATGCTGCCAAGGCCCACGGTTCCGTCATCCGTGTGGGAGTGAACG
GCGGTTCGCTGGAGCGCGATCTGGTGGAAAAATACTGCGGTCCCACGCCGGAGGCCATGGTGGAAAGCGCTTTGCGCCAT
GTGCGGATGCTGGAAGACCGCAATTTTGACAACATCAAGATTTCGCTCAAATCCTCTTCCGTAACGGATACTGTAACGGC
CTACCGGCTGCTCGGGCAGCAGTGTGAATATCCTTTGCATATCGGCGTGACAGAAGCGGGCACCATGCTGCGCGGCTCTG
TCAAGTCGTCGGTGGGCATAGGCATATTGCTGTGGCAGGGCATAGGTGATACTTTGCGGGTTTCGCTGACGGAAGATCCC
GTGGCCGAAATGGCTGTGGCATGGGAAATTCTGCGTTCGCTGGGGCTGCGTTTCCGCGGACCGGAAATCATATCCTGTCC
TACCTGCGGCCGTACCGAAATTGATCTCATTTCGCTGGCCGGAGAGATTGAACGCAGGCTGCAGAACGAAACCCAGAGTT
TCAAAGTGGCAGTCATGGGCTGTGTGGTCAACGGTCCGGGTGAAGCACGGGAAGCGGATATCGGTATTGCCGGCGGCCGT
GACAAGGGTATCATTTTCCGCCACGGCGAGGTTATCCGGACCGTGAAAGGCGGTTCCAACCTGCTGGCTGCTTTTAATGA
AGAACTGGATAAATTTTTGGCTGAACGTCAAGGACGGTAA

Upstream 100 bases:

>100_bases
CTGCGGATAAAATGCGTCCTGTCCGCCCCGATGCTTGGCTTGCCTGTGGAAATGCAGTATGAAGCCTGCATCCGTAACCG
AGCCAATAAGGATGCTTTAC

Downstream 100 bases:

>100_bases
AAAACAACAATGCGCTGGAGCAATTTTTATATTCCCACCCTTAAAGAAGCCCCTGCCGATGCGGAAGTGGTAAGCCACAA
GCTGCTGATCCGTGCGGGAA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTIARRKSREVTIGNVRIGGDNPVVVQSMTNTDTRDVEQTVEQINSLAEAGCEIVRVAVLNDDAAWAIRPIKDQVTVPLV
ADIHFDHRLAVSALEAGVDALRINPGNIGGQKNVDKVVDAAKAHGSVIRVGVNGGSLERDLVEKYCGPTPEAMVESALRH
VRMLEDRNFDNIKISLKSSSVTDTVTAYRLLGQQCEYPLHIGVTEAGTMLRGSVKSSVGIGILLWQGIGDTLRVSLTEDP
VAEMAVAWEILRSLGLRFRGPEIISCPTCGRTEIDLISLAGEIERRLQNETQSFKVAVMGCVVNGPGEAREADIGIAGGR
DKGIIFRHGEVIRTVKGGSNLLAAFNEELDKFLAERQGR

Sequences:

>Translated_359_residues
MTIARRKSREVTIGNVRIGGDNPVVVQSMTNTDTRDVEQTVEQINSLAEAGCEIVRVAVLNDDAAWAIRPIKDQVTVPLV
ADIHFDHRLAVSALEAGVDALRINPGNIGGQKNVDKVVDAAKAHGSVIRVGVNGGSLERDLVEKYCGPTPEAMVESALRH
VRMLEDRNFDNIKISLKSSSVTDTVTAYRLLGQQCEYPLHIGVTEAGTMLRGSVKSSVGIGILLWQGIGDTLRVSLTEDP
VAEMAVAWEILRSLGLRFRGPEIISCPTCGRTEIDLISLAGEIERRLQNETQSFKVAVMGCVVNGPGEAREADIGIAGGR
DKGIIFRHGEVIRTVKGGSNLLAAFNEELDKFLAERQGR
>Mature_358_residues
TIARRKSREVTIGNVRIGGDNPVVVQSMTNTDTRDVEQTVEQINSLAEAGCEIVRVAVLNDDAAWAIRPIKDQVTVPLVA
DIHFDHRLAVSALEAGVDALRINPGNIGGQKNVDKVVDAAKAHGSVIRVGVNGGSLERDLVEKYCGPTPEAMVESALRHV
RMLEDRNFDNIKISLKSSSVTDTVTAYRLLGQQCEYPLHIGVTEAGTMLRGSVKSSVGIGILLWQGIGDTLRVSLTEDPV
AEMAVAWEILRSLGLRFRGPEIISCPTCGRTEIDLISLAGEIERRLQNETQSFKVAVMGCVVNGPGEAREADIGIAGGRD
KGIIFRHGEVIRTVKGGSNLLAAFNEELDKFLAERQGR

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=327, Percent_Identity=53.5168195718654, Blast_Score=350, Evalue=8e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 38811; Mature: 38680

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIARRKSREVTIGNVRIGGDNPVVVQSMTNTDTRDVEQTVEQINSLAEAGCEIVRVAVL
CCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
NDDAAWAIRPIKDQVTVPLVADIHFDHRLAVSALEAGVDALRINPGNIGGQKNVDKVVDA
CCCCCEEEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHH
AKAHGSVIRVGVNGGSLERDLVEKYCGPTPEAMVESALRHVRMLEDRNFDNIKISLKSSS
HHCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
VTDTVTAYRLLGQQCEYPLHIGVTEAGTMLRGSVKSSVGIGILLWQGIGDTLRVSLTEDP
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHCCCCEEEEEECCCCCEEEEEECCCH
VAEMAVAWEILRSLGLRFRGPEIISCPTCGRTEIDLISLAGEIERRLQNETQSFKVAVMG
HHHHHHHHHHHHHCCCEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEEEE
CVVNGPGEAREADIGIAGGRDKGIIFRHGEVIRTVKGGSNLLAAFNEELDKFLAERQGR
EEECCCCCCCCCCEEEECCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIARRKSREVTIGNVRIGGDNPVVVQSMTNTDTRDVEQTVEQINSLAEAGCEIVRVAVL
CCCCCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
NDDAAWAIRPIKDQVTVPLVADIHFDHRLAVSALEAGVDALRINPGNIGGQKNVDKVVDA
CCCCCEEEEECCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHH
AKAHGSVIRVGVNGGSLERDLVEKYCGPTPEAMVESALRHVRMLEDRNFDNIKISLKSSS
HHCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
VTDTVTAYRLLGQQCEYPLHIGVTEAGTMLRGSVKSSVGIGILLWQGIGDTLRVSLTEDP
CHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCHHCCCCEEEEEECCCCCEEEEEECCCH
VAEMAVAWEILRSLGLRFRGPEIISCPTCGRTEIDLISLAGEIERRLQNETQSFKVAVMG
HHHHHHHHHHHHHCCCEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEEEE
CVVNGPGEAREADIGIAGGRDKGIIFRHGEVIRTVKGGSNLLAAFNEELDKFLAERQGR
EEECCCCCCCCCCEEEECCCCCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA