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The map label for this gene is proS

Identifier: 78357249

GI number: 78357249

Start: 2246007

End: 2247731

Strand: Reverse

Name: proS

Synonym: Dde_2206

Alternate gene names: 78357249

Gene position: 2247731-2246007 (Counterclockwise)

Preceding gene: 78357250

Following gene: 78357248

Centisome position: 60.26

GC content: 58.09

Gene sequence:

>1725_bases
ATGCGCTGGAGCAATTTTTATATTCCCACCCTTAAAGAAGCCCCTGCCGATGCGGAAGTGGTAAGCCACAAGCTGCTGAT
CCGTGCGGGAATGATCCGCAAGCTTACTTCGGGGATTTATATTTATCTGCCGCTGGGGCTGCGCTCTATCGAAAAGGCGG
CGTCCATCGTGCGTCAGGAGATGAACAGCGCCGGAGCACAGGAACTGCTTATGCCCATGGTGCAGCCTGCCGACCTGTGG
CAGGAGAGCGGCCGCTGGGATTTTTACGGCAAAGAACTGCTGCGTATTTCAGACCGGCACGGCCGCGATTACTGCCTTGG
GCCCACGCACGAAGAGGTCATTACCGACCTTGTGCGGGGTGAGGTGCGTTCGTACCGGCAGTTGCCTCTGAATCTGTATC
AGATCCAGACCAAGTTCCGCGACGAGATCCGCCCGCGTTTCGGGCTGATGCGCGGCCGTGAATTTATGATGAAGGATGCG
TACTCCTTTGACCGGGACGCACAGGGGCTGGATCTCAGCTACCGTGCCATGTACGAAGCCTATATGAAAATTTTCAGCCG
TATGGGGCTGAAATTCCGCGCAGTGGAAGCGGATTCCGGTTCCATAGGCGGCAGTTTTTCGCATGAGTTCATGGTGCTGG
CCGAAACAGGCGAAGACACTATAGCATTCTGCCACGACTGTGATTATTCCGCCAACGTCGAACGTGCGGAAGTAGTGTGC
ACGCTGGATGAATGCGATGCCCCCGTTGCCGGTGTGGAAGAGGTGCATACTCCTGACCGGCACACGGTGGAAGAAGTGTG
CGAGTTCATGAATGTGCCTGCCGCAGCGCTGATCAAAACCCTGCTGTATGTGGCCGACGGCGAGCCTGTTGCCGCTTTGG
TGCGCGGTGACCGCGAGCTTAACGAGGCCAAACTGAAAAACCTGCTGCGTGCCGATACTCTGGAGCTGGCCTCTCCTGAA
CAGGTCAGGGAATGGACCGGCGCACCGGTGGGATTTGCCGGCCCTGTGGGGCTGGGCGTGAAACGCCTGTTTGCCGATAA
TGAGCTGCGGCTTGCCACGGACTGGATAACCGGTGCCAACAAGGCAGACACGCACCTGCGGCATGTAAGCCTGAAACGCG
ACACCCGCCTTTCGGGATATGCCGACCTGCGCATGATTACGGACAAGGATCCCTGCCCGCGGTGTTCCGGCACGCTGGAG
CTTACCCGCGGTATTGAAGTGGGCCACGTTTTCAAGCTGGGCACAAAATACAGCGAGGCCATGGGCTGCAAGTTCCTTGA
CGAGAACGGCAAGGAACAGGTCATGCTGATGGGGTGCTACGGCATAGGCGTTTCCCGTGTGGTGGCCGCCTGTATTGAAC
AGAACAGTGACGAGAACGGCATTGTTTTTCCGCCCCCCATTGCCCCCTTTGAGGTGCTGCTGCTTAATCTGGACGGCAAG
AACGATGATGTGAACGCCAGAGTGGACGAGCTGTACGGGGCAGTGCAGGCAGCCGGATGCGATGTGCTGATGGACGACCG
TAATGAGCGTCCCGGGGTCAAGTTCAAGGATGCTGACCTGATAGGTGTTCCCATGCAGCTGGTTGTTGGCGGAAAAGGCC
TTGCCAGAGGCATTGTGGAGGCCAAGGACCGCCGTACCGGAGAAAAAACGGAACTGCCCGTGGACGGGTTTGCGCAGGCT
TTTGCCGCATGGCGCAGGCAGGTTCTGCAGGGCTGGGGTCTGTAA

Upstream 100 bases:

>100_bases
GGTTATCCGGACCGTGAAAGGCGGTTCCAACCTGCTGGCTGCTTTTAATGAAGAACTGGATAAATTTTTGGCTGAACGTC
AAGGACGGTAAAAAACAACA

Downstream 100 bases:

>100_bases
TACGCTGACAGTCTGCCGCCCGTGTCCCCCTTGCGGGATACGGGCGGTTTTGCATGGCGCTGCAAGGCGCATCTCAACGG
GGTCGACCGCGTGCGGACGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MRWSNFYIPTLKEAPADAEVVSHKLLIRAGMIRKLTSGIYIYLPLGLRSIEKAASIVRQEMNSAGAQELLMPMVQPADLW
QESGRWDFYGKELLRISDRHGRDYCLGPTHEEVITDLVRGEVRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFMMKDA
YSFDRDAQGLDLSYRAMYEAYMKIFSRMGLKFRAVEADSGSIGGSFSHEFMVLAETGEDTIAFCHDCDYSANVERAEVVC
TLDECDAPVAGVEEVHTPDRHTVEEVCEFMNVPAAALIKTLLYVADGEPVAALVRGDRELNEAKLKNLLRADTLELASPE
QVREWTGAPVGFAGPVGLGVKRLFADNELRLATDWITGANKADTHLRHVSLKRDTRLSGYADLRMITDKDPCPRCSGTLE
LTRGIEVGHVFKLGTKYSEAMGCKFLDENGKEQVMLMGCYGIGVSRVVAACIEQNSDENGIVFPPPIAPFEVLLLNLDGK
NDDVNARVDELYGAVQAAGCDVLMDDRNERPGVKFKDADLIGVPMQLVVGGKGLARGIVEAKDRRTGEKTELPVDGFAQA
FAAWRRQVLQGWGL

Sequences:

>Translated_574_residues
MRWSNFYIPTLKEAPADAEVVSHKLLIRAGMIRKLTSGIYIYLPLGLRSIEKAASIVRQEMNSAGAQELLMPMVQPADLW
QESGRWDFYGKELLRISDRHGRDYCLGPTHEEVITDLVRGEVRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFMMKDA
YSFDRDAQGLDLSYRAMYEAYMKIFSRMGLKFRAVEADSGSIGGSFSHEFMVLAETGEDTIAFCHDCDYSANVERAEVVC
TLDECDAPVAGVEEVHTPDRHTVEEVCEFMNVPAAALIKTLLYVADGEPVAALVRGDRELNEAKLKNLLRADTLELASPE
QVREWTGAPVGFAGPVGLGVKRLFADNELRLATDWITGANKADTHLRHVSLKRDTRLSGYADLRMITDKDPCPRCSGTLE
LTRGIEVGHVFKLGTKYSEAMGCKFLDENGKEQVMLMGCYGIGVSRVVAACIEQNSDENGIVFPPPIAPFEVLLLNLDGK
NDDVNARVDELYGAVQAAGCDVLMDDRNERPGVKFKDADLIGVPMQLVVGGKGLARGIVEAKDRRTGEKTELPVDGFAQA
FAAWRRQVLQGWGL
>Mature_574_residues
MRWSNFYIPTLKEAPADAEVVSHKLLIRAGMIRKLTSGIYIYLPLGLRSIEKAASIVRQEMNSAGAQELLMPMVQPADLW
QESGRWDFYGKELLRISDRHGRDYCLGPTHEEVITDLVRGEVRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFMMKDA
YSFDRDAQGLDLSYRAMYEAYMKIFSRMGLKFRAVEADSGSIGGSFSHEFMVLAETGEDTIAFCHDCDYSANVERAEVVC
TLDECDAPVAGVEEVHTPDRHTVEEVCEFMNVPAAALIKTLLYVADGEPVAALVRGDRELNEAKLKNLLRADTLELASPE
QVREWTGAPVGFAGPVGLGVKRLFADNELRLATDWITGANKADTHLRHVSLKRDTRLSGYADLRMITDKDPCPRCSGTLE
LTRGIEVGHVFKLGTKYSEAMGCKFLDENGKEQVMLMGCYGIGVSRVVAACIEQNSDENGIVFPPPIAPFEVLLLNLDGK
NDDVNARVDELYGAVQAAGCDVLMDDRNERPGVKFKDADLIGVPMQLVVGGKGLARGIVEAKDRRTGEKTELPVDGFAQA
FAAWRRQVLQGWGL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=253, Percent_Identity=45.0592885375494, Blast_Score=243, Evalue=4e-64,
Organism=Escherichia coli, GI1786392, Length=555, Percent_Identity=52.7927927927928, Blast_Score=570, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI115532348, Length=221, Percent_Identity=39.8190045248869, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=35.9550561797753, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=560, Percent_Identity=31.9642857142857, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24656200, Length=241, Percent_Identity=38.1742738589212, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_DESDG (Q30Z93)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388698.1
- ProteinModelPortal:   Q30Z93
- SMR:   Q30Z93
- STRING:   Q30Z93
- GeneID:   3757216
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2206
- NMPDR:   fig|207559.3.peg.2162
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   DDES207559:DDE_2206-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63933; Mature: 63933

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWSNFYIPTLKEAPADAEVVSHKLLIRAGMIRKLTSGIYIYLPLGLRSIEKAASIVRQE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHH
MNSAGAQELLMPMVQPADLWQESGRWDFYGKELLRISDRHGRDYCLGPTHEEVITDLVRG
HHCCCHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EVRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFMMKDAYSFDRDAQGLDLSYRAMYEA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
YMKIFSRMGLKFRAVEADSGSIGGSFSHEFMVLAETGEDTIAFCHDCDYSANVERAEVVC
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEE
TLDECDAPVAGVEEVHTPDRHTVEEVCEFMNVPAAALIKTLLYVADGEPVAALVRGDREL
EHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHHCCCHH
NEAKLKNLLRADTLELASPEQVREWTGAPVGFAGPVGLGVKRLFADNELRLATDWITGAN
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCC
KADTHLRHVSLKRDTRLSGYADLRMITDKDPCPRCSGTLELTRGIEVGHVFKLGTKYSEA
CCHHHHHEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCEEECCCHHHHH
MGCKFLDENGKEQVMLMGCYGIGVSRVVAACIEQNSDENGIVFPPPIAPFEVLLLNLDGK
HCCEEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEECCCC
NDDVNARVDELYGAVQAAGCDVLMDDRNERPGVKFKDADLIGVPMQLVVGGKGLARGIVE
CCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCEECCCCEEEECCCHHHHHHHH
AKDRRTGEKTELPVDGFAQAFAAWRRQVLQGWGL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRWSNFYIPTLKEAPADAEVVSHKLLIRAGMIRKLTSGIYIYLPLGLRSIEKAASIVRQE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHH
MNSAGAQELLMPMVQPADLWQESGRWDFYGKELLRISDRHGRDYCLGPTHEEVITDLVRG
HHCCCHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EVRSYRQLPLNLYQIQTKFRDEIRPRFGLMRGREFMMKDAYSFDRDAQGLDLSYRAMYEA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
YMKIFSRMGLKFRAVEADSGSIGGSFSHEFMVLAETGEDTIAFCHDCDYSANVERAEVVC
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEE
TLDECDAPVAGVEEVHTPDRHTVEEVCEFMNVPAAALIKTLLYVADGEPVAALVRGDREL
EHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEHHCCCHH
NEAKLKNLLRADTLELASPEQVREWTGAPVGFAGPVGLGVKRLFADNELRLATDWITGAN
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCC
KADTHLRHVSLKRDTRLSGYADLRMITDKDPCPRCSGTLELTRGIEVGHVFKLGTKYSEA
CCHHHHHEEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCEEECCCHHHHH
MGCKFLDENGKEQVMLMGCYGIGVSRVVAACIEQNSDENGIVFPPPIAPFEVLLLNLDGK
HCCEEECCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEECCCC
NDDVNARVDELYGAVQAAGCDVLMDDRNERPGVKFKDADLIGVPMQLVVGGKGLARGIVE
CCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCEECCCCEEEECCCHHHHHHHH
AKDRRTGEKTELPVDGFAQAFAAWRRQVLQGWGL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA