Click here to switch to the map view.

The map label for this gene is hynB1 [H]

Identifier: 78357180

GI number: 78357180

Start: 2184683

End: 2185636

Strand: Direct

Name: hynB1 [H]

Synonym: Dde_2137

Alternate gene names: 78357180

Gene position: 2184683-2185636 (Clockwise)

Preceding gene: 78357179

Following gene: 78357181

Centisome position: 58.57

GC content: 58.6

Gene sequence:

>954_bases
ATGAAGTTTTCTGTGGGTCTCGGCAAAGAGGGCGCAGAAGAGCGGTTGGCAAGCCGCGGCGTATCACGTCGTGACTTCCT
TAAATTCTGTTCCACCGTGGCCGTGGCCATGGGCATGGGGCCGGCTTTTGCGCCGGAAGTGGCCCGCGCCCTGACATCGG
GTAAACGGCCTTCGGTGGTGTACCTGCACAATGCCGAATGCACAGGCTGCTCGGAGTCTGTGCTGCGCGCATTCAACCCC
TACATCGACGAACTGCTGCTGGACACCATTTCGCTGGACTATCACGAAACCATAATGGCAGCTGCAGGCCATGCCGCCGA
AGAAGCGCTGCATCAGGCAGTACACGCTCCCGACGGATTCGTCGCCATTGTGGAAGGAGCCCTGCCGGTCAAGGACAACG
GCATATACGGTATGGTCGGCGGCAGAACCATGCTTGACATATGCTCCGACATCCTGCCCAGAGCAAAAGCCGTCATAGCC
TACGGCACCTGTGCCACGTTCGGCGGTGTGCAGGCGGCCGCCCCCAACCCCACCGGCGCAAAAGGCATCAACGATGCACT
GGCCGAGTTGGGTGTCAATGCCGTCAATATCTCCGGCTGCCCGCCCAACCCCTTTAACCTTGTGGGCACCATCGTCCACT
ACCTCCACCATGGCACCATTCCCGAACTTGATTCTCAAAACCGCCCCCTCATGTTCTTCGGCAAATCGGTGCATGAGCAA
TGCCCGCGCCTGAAGCATTTCGACGCCGGAGAATTCGCTCCGTCCTTCGATTCAGAAGAAGCCCGCAAAGGGTGGTGTCT
GTACGAACTGGGCTGCAAGGGTCCCATGACCATGAATAACTGCCCCAAAATCAAGTTCAACCAGACCAACTGGCCTGTTG
ATGCCGGCCACCCCTGCATCGGCTGCAGCGAACCTGATTTCTGGGATGCCATGAGCCCGTTCTACGAAATGTAG

Upstream 100 bases:

>100_bases
GCAAGGAGGCATCGGAGCGAATCTGCAAATGATTGCAACGCAATCAGGGCAATAGTGCTCCTGCAGATCTGATCCTTTCA
TTGCAGTAAGGAGGTTTCGT

Downstream 100 bases:

>100_bases
CGCAGGCCGGTACCGAAACGAATTGAACGCACTATAGCTTCAGAAGAGGAATCAATATGAGCGGTTGCAGAGCAAAAACA
GCCCCCGCCGGAATTCCGGT

Product: hydrogenase (NiFe) small subunit (hydA)

Products: NA

Alternate protein names: NiFe hydrogenlyase small chain 1 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKFSVGLGKEGAEERLASRGVSRRDFLKFCSTVAVAMGMGPAFAPEVARALTSGKRPSVVYLHNAECTGCSESVLRAFNP
YIDELLLDTISLDYHETIMAAAGHAAEEALHQAVHAPDGFVAIVEGALPVKDNGIYGMVGGRTMLDICSDILPRAKAVIA
YGTCATFGGVQAAAPNPTGAKGINDALAELGVNAVNISGCPPNPFNLVGTIVHYLHHGTIPELDSQNRPLMFFGKSVHEQ
CPRLKHFDAGEFAPSFDSEEARKGWCLYELGCKGPMTMNNCPKIKFNQTNWPVDAGHPCIGCSEPDFWDAMSPFYEM

Sequences:

>Translated_317_residues
MKFSVGLGKEGAEERLASRGVSRRDFLKFCSTVAVAMGMGPAFAPEVARALTSGKRPSVVYLHNAECTGCSESVLRAFNP
YIDELLLDTISLDYHETIMAAAGHAAEEALHQAVHAPDGFVAIVEGALPVKDNGIYGMVGGRTMLDICSDILPRAKAVIA
YGTCATFGGVQAAAPNPTGAKGINDALAELGVNAVNISGCPPNPFNLVGTIVHYLHHGTIPELDSQNRPLMFFGKSVHEQ
CPRLKHFDAGEFAPSFDSEEARKGWCLYELGCKGPMTMNNCPKIKFNQTNWPVDAGHPCIGCSEPDFWDAMSPFYEM
>Mature_317_residues
MKFSVGLGKEGAEERLASRGVSRRDFLKFCSTVAVAMGMGPAFAPEVARALTSGKRPSVVYLHNAECTGCSESVLRAFNP
YIDELLLDTISLDYHETIMAAAGHAAEEALHQAVHAPDGFVAIVEGALPVKDNGIYGMVGGRTMLDICSDILPRAKAVIA
YGTCATFGGVQAAAPNPTGAKGINDALAELGVNAVNISGCPPNPFNLVGTIVHYLHHGTIPELDSQNRPLMFFGKSVHEQ
CPRLKHFDAGEFAPSFDSEEARKGWCLYELGCKGPMTMNNCPKIKFNQTNWPVDAGHPCIGCSEPDFWDAMSPFYEM

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=297, Percent_Identity=46.4646464646465, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1787206, Length=309, Percent_Identity=41.1003236245955, Blast_Score=254, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 34041; Mature: 34041

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSVGLGKEGAEERLASRGVSRRDFLKFCSTVAVAMGMGPAFAPEVARALTSGKRPSVV
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEE
YLHNAECTGCSESVLRAFNPYIDELLLDTISLDYHETIMAAAGHAAEEALHQAVHAPDGF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
VAIVEGALPVKDNGIYGMVGGRTMLDICSDILPRAKAVIAYGTCATFGGVQAAAPNPTGA
EEEEECCCEECCCCEEEEECCCHHHHHHHHHHHHHHHEEEECCHHHHCCCCCCCCCCCCC
KGINDALAELGVNAVNISGCPPNPFNLVGTIVHYLHHGTIPELDSQNRPLMFFGKSVHEQ
CHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHH
CPRLKHFDAGEFAPSFDSEEARKGWCLYELGCKGPMTMNNCPKIKFNQTNWPVDAGHPCI
CCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCC
GCSEPDFWDAMSPFYEM
CCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKFSVGLGKEGAEERLASRGVSRRDFLKFCSTVAVAMGMGPAFAPEVARALTSGKRPSVV
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEE
YLHNAECTGCSESVLRAFNPYIDELLLDTISLDYHETIMAAAGHAAEEALHQAVHAPDGF
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
VAIVEGALPVKDNGIYGMVGGRTMLDICSDILPRAKAVIAYGTCATFGGVQAAAPNPTGA
EEEEECCCEECCCCEEEEECCCHHHHHHHHHHHHHHHEEEECCHHHHCCCCCCCCCCCCC
KGINDALAELGVNAVNISGCPPNPFNLVGTIVHYLHHGTIPELDSQNRPLMFFGKSVHEQ
CHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHH
CPRLKHFDAGEFAPSFDSEEARKGWCLYELGCKGPMTMNNCPKIKFNQTNWPVDAGHPCI
CCCCCCCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCC
GCSEPDFWDAMSPFYEM
CCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1479348 [H]