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The map label for this gene is hydB [H]

Identifier: 78357181

GI number: 78357181

Start: 2185693

End: 2187399

Strand: Direct

Name: hydB [H]

Synonym: Dde_2138

Alternate gene names: 78357181

Gene position: 2185693-2187399 (Clockwise)

Preceding gene: 78357180

Following gene: 78357182

Centisome position: 58.59

GC content: 57.82

Gene sequence:

>1707_bases
ATGAGCGGTTGCAGAGCAAAAACAGCCCCCGCCGGAATTCCGGTCACGCCCAAGAGCGACTACAGCGGTCCCATTGTAGT
GGACCCGATTACACGCATCGAAGGGCACCTGCGCATTGAGGTGGAGGTTGAAAACGGCCGTATTAAAAATGCATGGAGCA
GTTCACAGCTGTTCCGCGGACTCGAAATAATCCTCAAAGGACGCGACCCTCGCGACGCACAGCACTTCACTCAGCGCTCA
TGCGGTGTTTGCACCTATGTCCATGCCCTTGCCTCCACCCGCTGCGTGGACAACGCCGTGGGAGTGCGCATTCCCAAAAA
CGCAACGCTGATCCGCAACATGGTGCTGGCCGCGCAGTATATGCATGATCATATCGTGCACTTCTATCATCTGCATGCGC
TGGACTGGGTGGATGTAACCAACGCACTTAAAGCCGACCCCGCTAAAGCCGCCAAGCTGGCGAACACCATCTCGCCGCGC
AAAACCACGGCCGCTGACCTTAAAGCCGTACAGGAACGCCTGACCAAGTTTGTAGAAAGCGGCCAGCTCGGCCCCTTCAC
CAATGCCTACTTCCTCGGCGGGCATCCGTCTTACTATCTTGAGCCCGAAGTGGACCTCATTGCCACTGCCCACTATCTGG
AGGCCCTGCGTCTGCAGGTGAAAGCCGCCAGAGCCATGGCTGTGTTCGGTGCCAAAAACCCGCACACCCAGTTCACCGAC
GTAGGCGGTGTGACCTGCTATGCCGCACTGACCCCCGAACGCATTGCAGAGTTCACTGCTCTGTGGAAAGAAACAAAAGC
ATTCATCGACGAAGTATACATTCCGGACCTGCTGGCAGTGGCCGGTTCGTACAAGGACTGGTCGGCCATCGGCGGTACCA
GCAACTTCCTCACCTTCGGCGAGTTCCCCACAGATGAATACGACCTGAACAGCCGTTTCTTCCCGCAGGGAGCCATCTTC
AACCGCGACCTCTCGGCGGTGAAGGATTTCGACCCTTCGAGCATCATGGAGCATGTGCGCTACAGCTGGTATGAAGGCGA
CAAAGCGCACCACCCCTATGAAGGCGTGACCGAACCCAAATACACCGGCCTGCGGGGCGAAAACGACCGCTACTCGTGGA
TGAAAGCCCCCCGCTATCTTGAAGAGCCCATGGAAACGGGCCCGCTTGCACAGGTGCTTACCGCCTATGCCCGCGGCCAC
AAAGAAACAAAAGCGCTTGTTGACATGGTGCTGGGCAAACTGGGCGTGGGACCGGAAGCTCTCTTCTCCACGCTGGGACG
CACCGCCGCACGAGGCATTGAAACCGCCATCATCGCCGAAAAAACAGGTCAGATGATCAACGAGTTGAAAGACAACATTG
CCTCGGGTGACACGACGATCTACGAACCGTGGGAAATGCCCGACGAAGCGGAAGGTGTCGGATTTGTCACCGCACCGCGC
GGCGCTCTGTCGCATTGGATCAAAATCCGTGAAGGCAAAATTGAAAACTTCCAGCTCGTGGTTCCTTCCACGTGGTCGCT
GGGCCCGCGCTGTGCCAACGGCAAGCTGAGCCCTGTGGAAGAAGCGCTGGTGGGCACCCCCGTTGCCGACCCCAAACGTC
CTGTGGAAATCCTGCGTACCGTGCATTCGTTTGACCCCTGCATCGCCTGCGGCGTGCACGTCATCGATTCGCAGACCAAC
GAGGTGCATAAGTTCAAAGTGCTGTAA

Upstream 100 bases:

>100_bases
AACCTGATTTCTGGGATGCCATGAGCCCGTTCTACGAAATGTAGCGCAGGCCGGTACCGAAACGAATTGAACGCACTATA
GCTTCAGAAGAGGAATCAAT

Downstream 100 bases:

>100_bases
TTTTGCAGCGAAACAGCAAAATGAGAGGGAGCCCGGCCTTGCGCTGGACTCCCTTTTTTCCCTATAGTGGATGTGTATGG
AGCAGACAAAACGCATACTT

Product: periplasmic (NiFe) hydrogenase large subunit, isozyme 1

Products: NA

Alternate protein names: NiFe hydrogenlyase large chain [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSGCRAKTAPAGIPVTPKSDYSGPIVVDPITRIEGHLRIEVEVENGRIKNAWSSSQLFRGLEIILKGRDPRDAQHFTQRS
CGVCTYVHALASTRCVDNAVGVRIPKNATLIRNMVLAAQYMHDHIVHFYHLHALDWVDVTNALKADPAKAAKLANTISPR
KTTAADLKAVQERLTKFVESGQLGPFTNAYFLGGHPSYYLEPEVDLIATAHYLEALRLQVKAARAMAVFGAKNPHTQFTD
VGGVTCYAALTPERIAEFTALWKETKAFIDEVYIPDLLAVAGSYKDWSAIGGTSNFLTFGEFPTDEYDLNSRFFPQGAIF
NRDLSAVKDFDPSSIMEHVRYSWYEGDKAHHPYEGVTEPKYTGLRGENDRYSWMKAPRYLEEPMETGPLAQVLTAYARGH
KETKALVDMVLGKLGVGPEALFSTLGRTAARGIETAIIAEKTGQMINELKDNIASGDTTIYEPWEMPDEAEGVGFVTAPR
GALSHWIKIREGKIENFQLVVPSTWSLGPRCANGKLSPVEEALVGTPVADPKRPVEILRTVHSFDPCIACGVHVIDSQTN
EVHKFKVL

Sequences:

>Translated_568_residues
MSGCRAKTAPAGIPVTPKSDYSGPIVVDPITRIEGHLRIEVEVENGRIKNAWSSSQLFRGLEIILKGRDPRDAQHFTQRS
CGVCTYVHALASTRCVDNAVGVRIPKNATLIRNMVLAAQYMHDHIVHFYHLHALDWVDVTNALKADPAKAAKLANTISPR
KTTAADLKAVQERLTKFVESGQLGPFTNAYFLGGHPSYYLEPEVDLIATAHYLEALRLQVKAARAMAVFGAKNPHTQFTD
VGGVTCYAALTPERIAEFTALWKETKAFIDEVYIPDLLAVAGSYKDWSAIGGTSNFLTFGEFPTDEYDLNSRFFPQGAIF
NRDLSAVKDFDPSSIMEHVRYSWYEGDKAHHPYEGVTEPKYTGLRGENDRYSWMKAPRYLEEPMETGPLAQVLTAYARGH
KETKALVDMVLGKLGVGPEALFSTLGRTAARGIETAIIAEKTGQMINELKDNIASGDTTIYEPWEMPDEAEGVGFVTAPR
GALSHWIKIREGKIENFQLVVPSTWSLGPRCANGKLSPVEEALVGTPVADPKRPVEILRTVHSFDPCIACGVHVIDSQTN
EVHKFKVL
>Mature_567_residues
SGCRAKTAPAGIPVTPKSDYSGPIVVDPITRIEGHLRIEVEVENGRIKNAWSSSQLFRGLEIILKGRDPRDAQHFTQRSC
GVCTYVHALASTRCVDNAVGVRIPKNATLIRNMVLAAQYMHDHIVHFYHLHALDWVDVTNALKADPAKAAKLANTISPRK
TTAADLKAVQERLTKFVESGQLGPFTNAYFLGGHPSYYLEPEVDLIATAHYLEALRLQVKAARAMAVFGAKNPHTQFTDV
GGVTCYAALTPERIAEFTALWKETKAFIDEVYIPDLLAVAGSYKDWSAIGGTSNFLTFGEFPTDEYDLNSRFFPQGAIFN
RDLSAVKDFDPSSIMEHVRYSWYEGDKAHHPYEGVTEPKYTGLRGENDRYSWMKAPRYLEEPMETGPLAQVLTAYARGHK
ETKALVDMVLGKLGVGPEALFSTLGRTAARGIETAIIAEKTGQMINELKDNIASGDTTIYEPWEMPDEAEGVGFVTAPRG
ALSHWIKIREGKIENFQLVVPSTWSLGPRCANGKLSPVEEALVGTPVADPKRPVEILRTVHSFDPCIACGVHVIDSQTNE
VHKFKVL

Specific function: Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3 [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=43.7389770723104, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1787207, Length=581, Percent_Identity=44.0619621342513, Blast_Score=465, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 62713; Mature: 62582

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGCRAKTAPAGIPVTPKSDYSGPIVVDPITRIEGHLRIEVEVENGRIKNAWSSSQLFRG
CCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHCCCEEEEEEEECCCEECCCCCHHHHHHH
LEIILKGRDPRDAQHFTQRSCGVCTYVHALASTRCVDNAVGVRIPKNATLIRNMVLAAQY
HHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHH
MHDHIVHFYHLHALDWVDVTNALKADPAKAAKLANTISPRKTTAADLKAVQERLTKFVES
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GQLGPFTNAYFLGGHPSYYLEPEVDLIATAHYLEALRLQVKAARAMAVFGAKNPHTQFTD
CCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEC
VGGVTCYAALTPERIAEFTALWKETKAFIDEVYIPDLLAVAGSYKDWSAIGGTSNFLTFG
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEC
EFPTDEYDLNSRFFPQGAIFNRDLSAVKDFDPSSIMEHVRYSWYEGDKAHHPYEGVTEPK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YTGLRGENDRYSWMKAPRYLEEPMETGPLAQVLTAYARGHKETKALVDMVLGKLGVGPEA
CCCCCCCCCCHHHHHCCHHHHCHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHH
LFSTLGRTAARGIETAIIAEKTGQMINELKDNIASGDTTIYEPWEMPDEAEGVGFVTAPR
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEECCH
GALSHWIKIREGKIENFQLVVPSTWSLGPRCANGKLSPVEEALVGTPVADPKRPVEILRT
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VHSFDPCIACGVHVIDSQTNEVHKFKVL
HHHCCHHHHHCCCEECCCCCCCEEEECC
>Mature Secondary Structure 
SGCRAKTAPAGIPVTPKSDYSGPIVVDPITRIEGHLRIEVEVENGRIKNAWSSSQLFRG
CCCCCCCCCCCCCCCCCCCCCCCEEECCHHHCCCEEEEEEEECCCEECCCCCHHHHHHH
LEIILKGRDPRDAQHFTQRSCGVCTYVHALASTRCVDNAVGVRIPKNATLIRNMVLAAQY
HHHEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHH
MHDHIVHFYHLHALDWVDVTNALKADPAKAAKLANTISPRKTTAADLKAVQERLTKFVES
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
GQLGPFTNAYFLGGHPSYYLEPEVDLIATAHYLEALRLQVKAARAMAVFGAKNPHTQFTD
CCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEC
VGGVTCYAALTPERIAEFTALWKETKAFIDEVYIPDLLAVAGSYKDWSAIGGTSNFLTFG
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCEEEC
EFPTDEYDLNSRFFPQGAIFNRDLSAVKDFDPSSIMEHVRYSWYEGDKAHHPYEGVTEPK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
YTGLRGENDRYSWMKAPRYLEEPMETGPLAQVLTAYARGHKETKALVDMVLGKLGVGPEA
CCCCCCCCCCHHHHHCCHHHHCHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHH
LFSTLGRTAARGIETAIIAEKTGQMINELKDNIASGDTTIYEPWEMPDEAEGVGFVTAPR
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEECCH
GALSHWIKIREGKIENFQLVVPSTWSLGPRCANGKLSPVEEALVGTPVADPKRPVEILRT
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHH
VHSFDPCIACGVHVIDSQTNEVHKFKVL
HHHCCHHHHHCCCEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2269874; 9438867 [H]