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The map label for this gene is ispE

Identifier: 78357168

GI number: 78357168

Start: 2174156

End: 2175139

Strand: Direct

Name: ispE

Synonym: Dde_2125

Alternate gene names: 78357168

Gene position: 2174156-2175139 (Clockwise)

Preceding gene: 78357167

Following gene: 78357169

Centisome position: 58.28

GC content: 59.55

Gene sequence:

>984_bases
ATGCTTACCGTTCCGCAACAGACCATCCGCTCGGGGTGCAAGATAAACCTGTTTCTGGAGATAACGGGAGTGCGGCCTGA
CGGCTACCACGAACTGGTCACGCTGTTCTATCCGCTGTCAGAGCCTTTTGACCTGATGGAAATCAGCCCCGCAACGCATG
GAGTGACCGTGCTGAGCGAACGGGCCGACCTGTGCGGCGACAAAAACATCATCTGCAAGGCATGGCACACGTTCGCGGCA
GCAGGCGGTACTCCGCCGCCCATGCAGGTACGCCTTGCAAAAGGGGTACCCGACGGCGCCGGACTGGGCGGCGGCAGTGC
CAACGCGGCCGCAGTGCTCAAGCTGCTGAACACCGCGGGAGGAGCTCCACGTTTTTCTGACACCGCTCTGGCGAAAATAG
CTGCGCAGGTCGGTGCCGATGTGCCCTTTTTTCTGCATAACACGCCGTGCCTTGCAACAGGCATAGGTGAAAAACTTGTC
CCCGCGCCCCTTGACCTTTCCGGGTGGCACCTTGTACTAGTCTGCCCCGGAGTTCAGGTTTCCACTCCATGGGCTTATAA
CCGGTGGGACACTCTTTACCGGAGCGGGCTGCATCACCCGTGCGGGCAGTTGCCCGCCGCGCACGCACCGGCGGCCTGCG
CACAGTTGCAGACAACAGGGCCGGACCGCCCGGTACGGGCCGAAACAGGCTGCGCAGGCAGGAAAAAAAATACCTGCCGC
AGCTTGACAACTGAACGGCAAGCCGATAGTAAACCTGTCTCTCGTGCCCTGTGGCTCTTTAACAGCTTTGAAACGGTGGT
TTTCTCGGCCTATTCCGAACTGAGACAGCACAAGAACACGCTTCTTGCGCACGGCGCTTCGGCTGCTCTGATGAGCGGCA
GCGGTTCCAGTCTGTTCGGCCTTTTCAGGGACAAGGCTCAGGCCGAGGCGGCAATGCTCCACTTCTGTCAAAGAAGCATT
GCAGTATACGTCCACCGACTATAA

Upstream 100 bases:

>100_bases
GAGCGCTCAGGAAATTGTGACCAAAGCCATGGCCATTGCGGGCGAGCTGTGTGTTTTCACCAATGACCACCTGACCGTGG
AAAACGCGCGGCGGGCATAA

Downstream 100 bases:

>100_bases
TGCAGGGGTGTCGCCAAGTTGGTAAGGCAACGGGTTTTGGTCCCGTCATTCGAGGGTTCAAGTCCTTCCGCCCCTGCCAT
TTTTTTTGCGTGAACCATAA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLTVPQQTIRSGCKINLFLEITGVRPDGYHELVTLFYPLSEPFDLMEISPATHGVTVLSERADLCGDKNIICKAWHTFAA
AGGTPPPMQVRLAKGVPDGAGLGGGSANAAAVLKLLNTAGGAPRFSDTALAKIAAQVGADVPFFLHNTPCLATGIGEKLV
PAPLDLSGWHLVLVCPGVQVSTPWAYNRWDTLYRSGLHHPCGQLPAAHAPAACAQLQTTGPDRPVRAETGCAGRKKNTCR
SLTTERQADSKPVSRALWLFNSFETVVFSAYSELRQHKNTLLAHGASAALMSGSGSSLFGLFRDKAQAEAAMLHFCQRSI
AVYVHRL

Sequences:

>Translated_327_residues
MLTVPQQTIRSGCKINLFLEITGVRPDGYHELVTLFYPLSEPFDLMEISPATHGVTVLSERADLCGDKNIICKAWHTFAA
AGGTPPPMQVRLAKGVPDGAGLGGGSANAAAVLKLLNTAGGAPRFSDTALAKIAAQVGADVPFFLHNTPCLATGIGEKLV
PAPLDLSGWHLVLVCPGVQVSTPWAYNRWDTLYRSGLHHPCGQLPAAHAPAACAQLQTTGPDRPVRAETGCAGRKKNTCR
SLTTERQADSKPVSRALWLFNSFETVVFSAYSELRQHKNTLLAHGASAALMSGSGSSLFGLFRDKAQAEAAMLHFCQRSI
AVYVHRL
>Mature_327_residues
MLTVPQQTIRSGCKINLFLEITGVRPDGYHELVTLFYPLSEPFDLMEISPATHGVTVLSERADLCGDKNIICKAWHTFAA
AGGTPPPMQVRLAKGVPDGAGLGGGSANAAAVLKLLNTAGGAPRFSDTALAKIAAQVGADVPFFLHNTPCLATGIGEKLV
PAPLDLSGWHLVLVCPGVQVSTPWAYNRWDTLYRSGLHHPCGQLPAAHAPAACAQLQTTGPDRPVRAETGCAGRKKNTCR
SLTTERQADSKPVSRALWLFNSFETVVFSAYSELRQHKNTLLAHGASAALMSGSGSSLFGLFRDKAQAEAAMLHFCQRSI
AVYVHRL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=180, Percent_Identity=38.3333333333333, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_DESDG (Q30ZH4)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388617.1
- ProteinModelPortal:   Q30ZH4
- SMR:   Q30ZH4
- STRING:   Q30ZH4
- GeneID:   3757133
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2125
- NMPDR:   fig|207559.3.peg.2085
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   NHERSAF
- ProtClustDB:   PRK14615
- BioCyc:   DDES207559:DDE_2125-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 34850; Mature: 34850

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: ACT_SITE 14-14 ACT_SITE 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVPQQTIRSGCKINLFLEITGVRPDGYHELVTLFYPLSEPFDLMEISPATHGVTVLSE
CCCCCHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHH
RADLCGDKNIICKAWHTFAAAGGTPPPMQVRLAKGVPDGAGLGGGSANAAAVLKLLNTAG
HHHHCCCCCEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
GAPRFSDTALAKIAAQVGADVPFFLHNTPCLATGIGEKLVPAPLDLSGWHLVLVCPGVQV
CCCCCCHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCEE
STPWAYNRWDTLYRSGLHHPCGQLPAAHAPAACAQLQTTGPDRPVRAETGCAGRKKNTCR
CCCCCCHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
SLTTERQADSKPVSRALWLFNSFETVVFSAYSELRQHKNTLLAHGASAALMSGSGSSLFG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHH
LFRDKAQAEAAMLHFCQRSIAVYVHRL
HHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MLTVPQQTIRSGCKINLFLEITGVRPDGYHELVTLFYPLSEPFDLMEISPATHGVTVLSE
CCCCCHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHH
RADLCGDKNIICKAWHTFAAAGGTPPPMQVRLAKGVPDGAGLGGGSANAAAVLKLLNTAG
HHHHCCCCCEEEHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCC
GAPRFSDTALAKIAAQVGADVPFFLHNTPCLATGIGEKLVPAPLDLSGWHLVLVCPGVQV
CCCCCCHHHHHHHHHHHCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCEE
STPWAYNRWDTLYRSGLHHPCGQLPAAHAPAACAQLQTTGPDRPVRAETGCAGRKKNTCR
CCCCCCHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
SLTTERQADSKPVSRALWLFNSFETVVFSAYSELRQHKNTLLAHGASAALMSGSGSSLFG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHH
LFRDKAQAEAAMLHFCQRSIAVYVHRL
HHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA