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The map label for this gene is cysS

Identifier: 78357164

GI number: 78357164

Start: 2170127

End: 2171584

Strand: Direct

Name: cysS

Synonym: Dde_2121

Alternate gene names: 78357164

Gene position: 2170127-2171584 (Clockwise)

Preceding gene: 78357163

Following gene: 78357165

Centisome position: 58.18

GC content: 56.04

Gene sequence:

>1458_bases
ATGCAGCTATATAATACACTGACCCGAAAAAAAGAAAAATTTATCCCCCAGCGTGAAGGTAAAGCCAGCGTCTACGTGTG
CGGCATCACCGCCTACGACCTCTGCCATCTGGGCCATGCCAGATCTTCTGTGGCTTTTGATGTGCTGGTGCGCTACCTGC
GTCATACGGGGCTTGATGTGACTTTTGTCCGCAACTTTACCGACGTGGACGATAAAATCATCAAACGGGCCGGAGAGACA
GGGCTGACCAGCACCGAAGTGGCGGAAAAATATATGGCGGCCTTCCATGAAGACATGGACAGGCTGGGTTGCCTGCGCGC
CGACATAGAACCCCGCTGCACCCAGCATATCGGAGAAATGATAGCCCTGTGCGAAGACCTGATCAGCAAAGGCAAAGCCT
ACTCCACAGCCTCCGGCGATGTGTACTTCAGGGTCAGAAGCTTTGCGTCCTATGGTAAACTTTCCGGGCGCGATGTGGAT
GACATGCGCTCCGGAGCACGGGTGGCACCGGGCGAAGAAAAGGAAGATCCGCTGGATTTCGCTTTGTGGAAAAGTGCAAA
ACCCGGAGAGCCGTACTGGGAAAGCCCCTGGGGCAACGGCCGGCCCGGCTGGCATATCGAATGTTCGGCCATGAGCGAAA
AACACCTGCCGCTGCCTCTTGATATCCACGGCGGCGGTCAGGACCTTGTGTTTCCGCATCACGAAAACGAAATAGCCCAG
ACCGAAGCCGCCACCGGTAAAGAATTTGCACGCTACTGGGTGCATAACGGCTTTGTGCAGGTCAATGCTGAAAAAATGTC
AAAATCTCTCGGCAACTTCAGCACCATACGCGATATTCTGCAAGGATACCTGCCGGAAACTTTGCGCTATTTCCTGCTGA
CCAAGCATTACAGAAGCCCCATAGACTTCACCTTTGACGGTATGGATGAAGCGGAAAAAAACCTGAGGCGCATCTACCAG
ACGCTGAATCTTGTGGAAAACGAACTGCAGAAGACCAAGTGGTCCGCAGCGCCCCTGCCCGAAGAAGTACTCAGCGAAAT
GGATGAAACAGAACGCGCGTGGAACGAAGCCATGGAAGATGACCTGAACACAGCCGCAGCGCTGGGGCATATCTTCGGTC
TCGTCCGTCTGGTAAACCGCATCATTGAAGACAAGACCATGCGCAAAAGCGCGCAGGCCCGTGATGCACTGCTGCGCATG
CAGAGCATGATGGCCCGCTGGGGCGCAGTGCTCGGACTGTTTACCCGGCAGCCGGCAGAGTTTCTGCGCGAAATGAGAGA
CTGCCGCGCAGCCCGCAGGGACGTTGATACCGCCCGCGTTGAAACGCTGCTTCTCGAACGGCAGGAAGCACGCAAGGCCA
AGGACTTTGAACGGTCGGACGCCATCCGCGAAGAGCTGGCGCGTATGGGCGTCGAAGTACAGGACACCCCCGCAGGAGCC
GCCTGGGACATAGCCTGA

Upstream 100 bases:

>100_bases
TTGAAACCGAGTTTGAAGGCGGGCGGCATCAGCGCCGCATCGACCTGTTTGATTCTGCCAGCGCCTGAAAACAGCTGGTT
CCTTCCGGAGACGATACAGA

Downstream 100 bases:

>100_bases
TATGGCAGCACCCGAGTTCACCATTCCGCGTTACCGGCGCCCTGTGCGGCGCCGGTACGCGGCTCTGCTAATCGCAGTGC
TCTGCCTGAACCTTGTTCTG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MQLYNTLTRKKEKFIPQREGKASVYVCGITAYDLCHLGHARSSVAFDVLVRYLRHTGLDVTFVRNFTDVDDKIIKRAGET
GLTSTEVAEKYMAAFHEDMDRLGCLRADIEPRCTQHIGEMIALCEDLISKGKAYSTASGDVYFRVRSFASYGKLSGRDVD
DMRSGARVAPGEEKEDPLDFALWKSAKPGEPYWESPWGNGRPGWHIECSAMSEKHLPLPLDIHGGGQDLVFPHHENEIAQ
TEAATGKEFARYWVHNGFVQVNAEKMSKSLGNFSTIRDILQGYLPETLRYFLLTKHYRSPIDFTFDGMDEAEKNLRRIYQ
TLNLVENELQKTKWSAAPLPEEVLSEMDETERAWNEAMEDDLNTAAALGHIFGLVRLVNRIIEDKTMRKSAQARDALLRM
QSMMARWGAVLGLFTRQPAEFLREMRDCRAARRDVDTARVETLLLERQEARKAKDFERSDAIREELARMGVEVQDTPAGA
AWDIA

Sequences:

>Translated_485_residues
MQLYNTLTRKKEKFIPQREGKASVYVCGITAYDLCHLGHARSSVAFDVLVRYLRHTGLDVTFVRNFTDVDDKIIKRAGET
GLTSTEVAEKYMAAFHEDMDRLGCLRADIEPRCTQHIGEMIALCEDLISKGKAYSTASGDVYFRVRSFASYGKLSGRDVD
DMRSGARVAPGEEKEDPLDFALWKSAKPGEPYWESPWGNGRPGWHIECSAMSEKHLPLPLDIHGGGQDLVFPHHENEIAQ
TEAATGKEFARYWVHNGFVQVNAEKMSKSLGNFSTIRDILQGYLPETLRYFLLTKHYRSPIDFTFDGMDEAEKNLRRIYQ
TLNLVENELQKTKWSAAPLPEEVLSEMDETERAWNEAMEDDLNTAAALGHIFGLVRLVNRIIEDKTMRKSAQARDALLRM
QSMMARWGAVLGLFTRQPAEFLREMRDCRAARRDVDTARVETLLLERQEARKAKDFERSDAIREELARMGVEVQDTPAGA
AWDIA
>Mature_485_residues
MQLYNTLTRKKEKFIPQREGKASVYVCGITAYDLCHLGHARSSVAFDVLVRYLRHTGLDVTFVRNFTDVDDKIIKRAGET
GLTSTEVAEKYMAAFHEDMDRLGCLRADIEPRCTQHIGEMIALCEDLISKGKAYSTASGDVYFRVRSFASYGKLSGRDVD
DMRSGARVAPGEEKEDPLDFALWKSAKPGEPYWESPWGNGRPGWHIECSAMSEKHLPLPLDIHGGGQDLVFPHHENEIAQ
TEAATGKEFARYWVHNGFVQVNAEKMSKSLGNFSTIRDILQGYLPETLRYFLLTKHYRSPIDFTFDGMDEAEKNLRRIYQ
TLNLVENELQKTKWSAAPLPEEVLSEMDETERAWNEAMEDDLNTAAALGHIFGLVRLVNRIIEDKTMRKSAQARDALLRM
QSMMARWGAVLGLFTRQPAEFLREMRDCRAARRDVDTARVETLLLERQEARKAKDFERSDAIREELARMGVEVQDTPAGA
AWDIA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=392, Percent_Identity=41.3265306122449, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI10835051, Length=347, Percent_Identity=35.4466858789625, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI62240992, Length=347, Percent_Identity=35.4466858789625, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI21269877, Length=347, Percent_Identity=35.4466858789625, Blast_Score=189, Evalue=7e-48,
Organism=Homo sapiens, GI303304987, Length=347, Percent_Identity=35.4466858789625, Blast_Score=189, Evalue=7e-48,
Organism=Escherichia coli, GI1786737, Length=482, Percent_Identity=46.6804979253112, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17509795, Length=362, Percent_Identity=33.9779005524862, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17509797, Length=362, Percent_Identity=33.9779005524862, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324082, Length=340, Percent_Identity=32.9411764705882, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI19922218, Length=508, Percent_Identity=33.4645669291339, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI19922364, Length=423, Percent_Identity=31.6784869976359, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_DESDG (Q30ZH8)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388613.1
- ProteinModelPortal:   Q30ZH8
- SMR:   Q30ZH8
- STRING:   Q30ZH8
- GeneID:   3757129
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2121
- NMPDR:   fig|207559.3.peg.2081
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   DDES207559:DDE_2121-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55156; Mature: 55156

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 268-268

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYNTLTRKKEKFIPQREGKASVYVCGITAYDLCHLGHARSSVAFDVLVRYLRHTGLDV
CCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
TFVRNFTDVDDKIIKRAGETGLTSTEVAEKYMAAFHEDMDRLGCLRADIEPRCTQHIGEM
EEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IALCEDLISKGKAYSTASGDVYFRVRSFASYGKLSGRDVDDMRSGARVAPGEEKEDPLDF
HHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCHH
ALWKSAKPGEPYWESPWGNGRPGWHIECSAMSEKHLPLPLDIHGGGQDLVFPHHENEIAQ
HHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCHHHH
TEAATGKEFARYWVHNGFVQVNAEKMSKSLGNFSTIRDILQGYLPETLRYFLLTKHYRSP
HHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDFTFDGMDEAEKNLRRIYQTLNLVENELQKTKWSAAPLPEEVLSEMDETERAWNEAMED
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLNTAAALGHIFGLVRLVNRIIEDKTMRKSAQARDALLRMQSMMARWGAVLGLFTRQPAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FLREMRDCRAARRDVDTARVETLLLERQEARKAKDFERSDAIREELARMGVEVQDTPAGA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
AWDIA
CCCCC
>Mature Secondary Structure
MQLYNTLTRKKEKFIPQREGKASVYVCGITAYDLCHLGHARSSVAFDVLVRYLRHTGLDV
CCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCE
TFVRNFTDVDDKIIKRAGETGLTSTEVAEKYMAAFHEDMDRLGCLRADIEPRCTQHIGEM
EEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IALCEDLISKGKAYSTASGDVYFRVRSFASYGKLSGRDVDDMRSGARVAPGEEKEDPLDF
HHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCHH
ALWKSAKPGEPYWESPWGNGRPGWHIECSAMSEKHLPLPLDIHGGGQDLVFPHHENEIAQ
HHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCHHHH
TEAATGKEFARYWVHNGFVQVNAEKMSKSLGNFSTIRDILQGYLPETLRYFLLTKHYRSP
HHHHHHHHHHHHHHHCCEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IDFTFDGMDEAEKNLRRIYQTLNLVENELQKTKWSAAPLPEEVLSEMDETERAWNEAMED
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLNTAAALGHIFGLVRLVNRIIEDKTMRKSAQARDALLRMQSMMARWGAVLGLFTRQPAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FLREMRDCRAARRDVDTARVETLLLERQEARKAKDFERSDAIREELARMGVEVQDTPAGA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCC
AWDIA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA