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The map label for this gene is rsmE [H]

Identifier: 78357040

GI number: 78357040

Start: 2040355

End: 2041071

Strand: Direct

Name: rsmE [H]

Synonym: Dde_1997

Alternate gene names: 78357040

Gene position: 2040355-2041071 (Clockwise)

Preceding gene: 78357039

Following gene: 304570584

Centisome position: 54.7

GC content: 54.67

Gene sequence:

>717_bases
ATGAGAACGTTTTTTCTTTCACCGGACGAATGGGACAGACAGTGCACTCTTAGAGGCTCCGAAGCACACCATATGATAAA
GGTGCTGCGGATGCGTGCAGGAGACACCTGCCGGATTCTGGACGGCAAAGGCCGTGAAGGCTTATTCATAATACGCGGTA
TGGATAAAAACAGTGTGCAGCTGGAGCTTGCAGAAACTGTCATGCACCCCGAACCGCATTCGCGCGCGTATATTGCCCTG
GGCTGGGGCAAATCAGTAAGGCGCAGCTGGATTTTTGAAAAGGCAGTGGAGCTGGAAGCTGCGGGGATCTGGCTGTGGCA
GGCGGCACGCAGCCAGTCAAAAATGCCTGACATGACAAAAGAAAGCTGGCACAGCCAGCTTGTTGCAGGTGCAAAGCAAT
GCGTCAACCCATGGATTCCCGAACTGAAATCGGTGGCCGCTGGGGTGACCGGACTGGTCGAACTGGCTCAGGGCTTTGAC
CGTAAATTCATTCTGTGGGAAGGAGAAGCCAGAGAAGCGTTGCTGTCTGGCGAAACCCTCGGCACTTCAGGTGACACTCT
GTTTGTAATAGGCCCCGAAGGCGGCTTCGCAGATGACGAGGTCAGCCTGCTGAAAGAAAGCGGCTTTGCAGCCGCCAGTC
TGGGCAACAGAATACTGCGCTGGGAAACGGCGGCACTTCTTTGCCTTGGGCTGCACTTCTGGAGCAAACAACGATAA

Upstream 100 bases:

>100_bases
TTTATAAAAAAGGCACAGCGCGCATCAAGCTGTAACCACAGAATTCCGAAGGCTCCACCTTTTACAGGCACGGCTTTCCG
TGCCTTTTTTCATAGAGCTT

Downstream 100 bases:

>100_bases
ACCGGAGCATTCCCCTGTTACACCTGACACAACCACAGCAGCCCCTGCCGGAAAAAATCCGGCCGGCCGATCTGGAAGGC
TTTGTCGGACAAACACACCT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRTFFLSPDEWDRQCTLRGSEAHHMIKVLRMRAGDTCRILDGKGREGLFIIRGMDKNSVQLELAETVMHPEPHSRAYIAL
GWGKSVRRSWIFEKAVELEAAGIWLWQAARSQSKMPDMTKESWHSQLVAGAKQCVNPWIPELKSVAAGVTGLVELAQGFD
RKFILWEGEAREALLSGETLGTSGDTLFVIGPEGGFADDEVSLLKESGFAAASLGNRILRWETAALLCLGLHFWSKQR

Sequences:

>Translated_238_residues
MRTFFLSPDEWDRQCTLRGSEAHHMIKVLRMRAGDTCRILDGKGREGLFIIRGMDKNSVQLELAETVMHPEPHSRAYIAL
GWGKSVRRSWIFEKAVELEAAGIWLWQAARSQSKMPDMTKESWHSQLVAGAKQCVNPWIPELKSVAAGVTGLVELAQGFD
RKFILWEGEAREALLSGETLGTSGDTLFVIGPEGGFADDEVSLLKESGFAAASLGNRILRWETAALLCLGLHFWSKQR
>Mature_238_residues
MRTFFLSPDEWDRQCTLRGSEAHHMIKVLRMRAGDTCRILDGKGREGLFIIRGMDKNSVQLELAETVMHPEPHSRAYIAL
GWGKSVRRSWIFEKAVELEAAGIWLWQAARSQSKMPDMTKESWHSQLVAGAKQCVNPWIPELKSVAAGVTGLVELAQGFD
RKFILWEGEAREALLSGETLGTSGDTLFVIGPEGGFADDEVSLLKESGFAAASLGNRILRWETAALLCLGLHFWSKQR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26571; Mature: 26571

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFFLSPDEWDRQCTLRGSEAHHMIKVLRMRAGDTCRILDGKGREGLFIIRGMDKNSVQ
CCEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCEE
LELAETVMHPEPHSRAYIALGWGKSVRRSWIFEKAVELEAAGIWLWQAARSQSKMPDMTK
EEEHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHCCCCCHHH
ESWHSQLVAGAKQCVNPWIPELKSVAAGVTGLVELAQGFDRKFILWEGEAREALLSGETL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCC
GTSGDTLFVIGPEGGFADDEVSLLKESGFAAASLGNRILRWETAALLCLGLHFWSKQR
CCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTFFLSPDEWDRQCTLRGSEAHHMIKVLRMRAGDTCRILDGKGREGLFIIRGMDKNSVQ
CCEEECCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCCEE
LELAETVMHPEPHSRAYIALGWGKSVRRSWIFEKAVELEAAGIWLWQAARSQSKMPDMTK
EEEHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHCCCCCHHH
ESWHSQLVAGAKQCVNPWIPELKSVAAGVTGLVELAQGFDRKFILWEGEAREALLSGETL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCC
GTSGDTLFVIGPEGGFADDEVSLLKESGFAAASLGNRILRWETAALLCLGLHFWSKQR
CCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]