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The map label for this gene is pleD [H]

Identifier: 78356877

GI number: 78356877

Start: 1883494

End: 1885071

Strand: Reverse

Name: pleD [H]

Synonym: Dde_1834

Alternate gene names: 78356877

Gene position: 1885071-1883494 (Counterclockwise)

Preceding gene: 78356880

Following gene: 78356876

Centisome position: 50.53

GC content: 53.74

Gene sequence:

>1578_bases
ATGAGTTCGTTACTCAACATACTGATTTGCGATATTTTTATTTTCACGGCGCAAAGGTTGCATGCAAGATGGCAAGAAGA
ATCACCCGATGATATCGGGGGGGAGGATATATACGTGAAGCGTAAGAATCCGCTTGTCATGTGGGGCATAGCTCTTGAAA
CAGAAGCGGCAGCCTTTCTGGAACAGGCAGCCTGTAGCGGATGCGTGCTGCATAACTGGGATGGGCGTATGCCTGATAAC
AGTGCGCTGGAATCCGATGAACCGGTTCTGGTCTGGGTTTCACAGCGGGGATGGGATGCTGTGTCTGCTCTGCCGGACGA
AGAAATGCAGTTTCTGCATCTTGTTCCCAGAGTTCTGTTGTTGCAGCAGGATGTTTCACCGGAGTATGTGGAACGGGCGC
TTGATGCCGGTTTCACCGACATTCTGAAGGCTCCTTTTTCTGAACCCAGAGTACGCGAAGTTTTTCTGCGTGCGGCGGAA
ATGCAGAACCTGTACGACGATATCATCCGTATGACCCGCGAGATATGCCTTGAACGTGAGGTGCTCGCACGAAAGAATGA
TATTCTGGAGTTTATCGTCACCTTTTTGTCCCGTGCCACAGAAAGTCTTGATCCTGTGGAAATTCTGAGCAACGCCCGGG
AAGATTTGAGCATGCTGCTGCCGCTGCGCGCGTTGCATGCCGCTTTGTGGCATCCGCAGCAGAAAGACTCCCTTGAAGCG
GAAGTGTTTCTGGGGGTGGGCGAGCACACGCAGGCCGGTCTTGAGTGGAGTCAGATGTTGCTTAAGGCGGCGGCAAAGCT
GAGCGGCGTGCCTGTTTCGGGCTACACGGTGGAGCAGCTTGGGCAGGGAGGGGCGGAAGATGCTCCCGCACCGGGAAAAG
TCATTCTGCTGCCTTTGAAAATGAGGGATGAGACGCTGGGCTGTCTTGCTCTGCTTACAGAGGATGACTGCCAGCTTGGC
AAAGATCAGGTTCAGGTGCTGCATTCTGCCATGCGCCATCTGGCACTGGCGGTAAAAAATGCCCTGCTTTTCCGTGAAAT
GCGTTTGCAGGCTGACCACGACGGGCTGACCCAGCTGCACAACAGACGCCATTTCGATACACGGCTGCGTGAGGAACTGG
ACAGGCACGGTCGCTACAGCATGCCGCTTACCCTGATGATGCTGGATATCGACTATTTTAAGCAGATCAATGACCGTTTT
GGCCATCAGGCCGGCGATGCCGTTTTGCGGGAACTTGCCAGTGTGCTGCGTGAAACCATCAGAACCACGGATTACTGCGC
ACGGTATGGCGGTGAAGAGTTTGTGGCCATATTGCCTCATACCGATGAACAGCAGGCACGCGTGCTGGCGGAACGGTTGA
GAAAAAATATAGAAAGCAGAGTGTTCCGTCACCATGGAAAAACAATACAGGCGACAGTGAGCATAGGGCTGACAGGCTTC
AAGCCGGGTGCGCTTACTCCGCGTGACGAACTGGTGCGCGAAGCCGACGCGGCGTTGTATCTGGCCAAGGCCAACGGCAG
AAATGTTGTGTGCACTTTTGCAGGCAGCTGCAATGAAAAGGTAAAAAAGGCCCAGTAA

Upstream 100 bases:

>100_bases
ATGCGCTTGCGGCAAGGTGTACTGTGCATGCTTTTGAAAAGACACAGAACAGGCCGGTTGTCAAACAGAGACAGTATTTT
GACGGTACCCTTGTGGGAAA

Downstream 100 bases:

>100_bases
GCCGCACTTGACCGTCTGCCGGGAATCCGGCATTTCTCCGGATCATATCAGACAGAGCTTTTGCGCTCTGTCTTTTCATT
TGCCGGAGGTCATGTTTTGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSSLLNILICDIFIFTAQRLHARWQEESPDDIGGEDIYVKRKNPLVMWGIALETEAAAFLEQAACSGCVLHNWDGRMPDN
SALESDEPVLVWVSQRGWDAVSALPDEEMQFLHLVPRVLLLQQDVSPEYVERALDAGFTDILKAPFSEPRVREVFLRAAE
MQNLYDDIIRMTREICLEREVLARKNDILEFIVTFLSRATESLDPVEILSNAREDLSMLLPLRALHAALWHPQQKDSLEA
EVFLGVGEHTQAGLEWSQMLLKAAAKLSGVPVSGYTVEQLGQGGAEDAPAPGKVILLPLKMRDETLGCLALLTEDDCQLG
KDQVQVLHSAMRHLALAVKNALLFREMRLQADHDGLTQLHNRRHFDTRLREELDRHGRYSMPLTLMMLDIDYFKQINDRF
GHQAGDAVLRELASVLRETIRTTDYCARYGGEEFVAILPHTDEQQARVLAERLRKNIESRVFRHHGKTIQATVSIGLTGF
KPGALTPRDELVREADAALYLAKANGRNVVCTFAGSCNEKVKKAQ

Sequences:

>Translated_525_residues
MSSLLNILICDIFIFTAQRLHARWQEESPDDIGGEDIYVKRKNPLVMWGIALETEAAAFLEQAACSGCVLHNWDGRMPDN
SALESDEPVLVWVSQRGWDAVSALPDEEMQFLHLVPRVLLLQQDVSPEYVERALDAGFTDILKAPFSEPRVREVFLRAAE
MQNLYDDIIRMTREICLEREVLARKNDILEFIVTFLSRATESLDPVEILSNAREDLSMLLPLRALHAALWHPQQKDSLEA
EVFLGVGEHTQAGLEWSQMLLKAAAKLSGVPVSGYTVEQLGQGGAEDAPAPGKVILLPLKMRDETLGCLALLTEDDCQLG
KDQVQVLHSAMRHLALAVKNALLFREMRLQADHDGLTQLHNRRHFDTRLREELDRHGRYSMPLTLMMLDIDYFKQINDRF
GHQAGDAVLRELASVLRETIRTTDYCARYGGEEFVAILPHTDEQQARVLAERLRKNIESRVFRHHGKTIQATVSIGLTGF
KPGALTPRDELVREADAALYLAKANGRNVVCTFAGSCNEKVKKAQ
>Mature_524_residues
SSLLNILICDIFIFTAQRLHARWQEESPDDIGGEDIYVKRKNPLVMWGIALETEAAAFLEQAACSGCVLHNWDGRMPDNS
ALESDEPVLVWVSQRGWDAVSALPDEEMQFLHLVPRVLLLQQDVSPEYVERALDAGFTDILKAPFSEPRVREVFLRAAEM
QNLYDDIIRMTREICLEREVLARKNDILEFIVTFLSRATESLDPVEILSNAREDLSMLLPLRALHAALWHPQQKDSLEAE
VFLGVGEHTQAGLEWSQMLLKAAAKLSGVPVSGYTVEQLGQGGAEDAPAPGKVILLPLKMRDETLGCLALLTEDDCQLGK
DQVQVLHSAMRHLALAVKNALLFREMRLQADHDGLTQLHNRRHFDTRLREELDRHGRYSMPLTLMMLDIDYFKQINDRFG
HQAGDAVLRELASVLRETIRTTDYCARYGGEEFVAILPHTDEQQARVLAERLRKNIESRVFRHHGKTIQATVSIGLTGFK
PGALTPRDELVREADAALYLAKANGRNVVCTFAGSCNEKVKKAQ

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=37.0165745856354, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI145693134, Length=177, Percent_Identity=36.7231638418079, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=37.5722543352601, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=35.5421686746988, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788381, Length=171, Percent_Identity=35.0877192982456, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=37.0588235294118, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=35.5932203389831, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=174, Percent_Identity=35.632183908046, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=38.75, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=26.8571428571429, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=30.6748466257669, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081977, Length=214, Percent_Identity=25.2336448598131, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 59120; Mature: 58989

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLNILICDIFIFTAQRLHARWQEESPDDIGGEDIYVKRKNPLVMWGIALETEAAAFL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEEEHHHHHHHH
EQAACSGCVLHNWDGRMPDNSALESDEPVLVWVSQRGWDAVSALPDEEMQFLHLVPRVLL
HHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHH
LQQDVSPEYVERALDAGFTDILKAPFSEPRVREVFLRAAEMQNLYDDIIRMTREICLERE
HHCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLARKNDILEFIVTFLSRATESLDPVEILSNAREDLSMLLPLRALHAALWHPQQKDSLEA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCE
EVFLGVGEHTQAGLEWSQMLLKAAAKLSGVPVSGYTVEQLGQGGAEDAPAPGKVILLPLK
EEEEECCCCCHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEC
MRDETLGCLALLTEDDCQLGKDQVQVLHSAMRHLALAVKNALLFREMRLQADHDGLTQLH
CCCCCHHEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NRRHFDTRLREELDRHGRYSMPLTLMMLDIDYFKQINDRFGHQAGDAVLRELASVLRETI
HCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RTTDYCARYGGEEFVAILPHTDEQQARVLAERLRKNIESRVFRHHGKTIQATVSIGLTGF
HHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECC
KPGALTPRDELVREADAALYLAKANGRNVVCTFAGSCNEKVKKAQ
CCCCCCCHHHHHHHHHHEEEEEECCCCEEEEEECCCCHHHHHCCC
>Mature Secondary Structure 
SSLLNILICDIFIFTAQRLHARWQEESPDDIGGEDIYVKRKNPLVMWGIALETEAAAFL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEEEHHHHHHHH
EQAACSGCVLHNWDGRMPDNSALESDEPVLVWVSQRGWDAVSALPDEEMQFLHLVPRVLL
HHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHH
LQQDVSPEYVERALDAGFTDILKAPFSEPRVREVFLRAAEMQNLYDDIIRMTREICLERE
HHCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLARKNDILEFIVTFLSRATESLDPVEILSNAREDLSMLLPLRALHAALWHPQQKDSLEA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCE
EVFLGVGEHTQAGLEWSQMLLKAAAKLSGVPVSGYTVEQLGQGGAEDAPAPGKVILLPLK
EEEEECCCCCHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEEC
MRDETLGCLALLTEDDCQLGKDQVQVLHSAMRHLALAVKNALLFREMRLQADHDGLTQLH
CCCCCHHEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
NRRHFDTRLREELDRHGRYSMPLTLMMLDIDYFKQINDRFGHQAGDAVLRELASVLRETI
HCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RTTDYCARYGGEEFVAILPHTDEQQARVLAERLRKNIESRVFRHHGKTIQATVSIGLTGF
HHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECC
KPGALTPRDELVREADAALYLAKANGRNVVCTFAGSCNEKVKKAQ
CCCCCCCHHHHHHHHHHEEEEEECCCCEEEEEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA