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The map label for this gene is miaA

Identifier: 78356827

GI number: 78356827

Start: 1835564

End: 1836496

Strand: Direct

Name: miaA

Synonym: Dde_1784

Alternate gene names: 78356827

Gene position: 1835564-1836496 (Clockwise)

Preceding gene: 78356826

Following gene: 78356828

Centisome position: 49.21

GC content: 57.98

Gene sequence:

>933_bases
ATGTCAGAGGCTTCCGTGATTCCTGTAGTATGTATTGTCGGCCCTACCGGCGCGGGTAAGACTGCCGCGTCACTGCATCT
TGCCGCCGTGTTCGACGGCGCGGTAATTAACTGCGACTCACGGCAGGTGTACAAAGATTTTCCTGTCATCACTGCCCAGC
CTACGCTTGAGGAGCAGGCATCCTGTCCGCACCATCTGTACGGATTCATGGGCATACAGGAAAAGATGAGTGCCGGTGTT
TTTCTTGATATGGCCTGCCCGATCATCAAAGAAGAGCACGCACACGGCAGGCTTCCTCTGCTTGTGGGAGGAACAGGCCT
GTATCTGCGTGCGCTGCTCGACGGCATAGCCGCCATACCCGACATTCCCGAAACGGTATCGCAGAAATGGCAGGAGAGAT
GTGCAGCCCGCGGGGCGCCGGCACTGCACAGGCTGCTTGAAGCGCAGGACCCGCAGACAGCAGAGCGGCTTCACCCCAAT
GACAGCCAGAGGATAGTGCGTGCGCTGGAAGTACAGGAGGCCACAGGCAGACCACTTTCATGGTGGCATGCCCAGCCTGT
TCCTCCGTCGCCGTATCGTGCTGTAAAAATAGGGGTCAGCGCAACGCTGGACGGACTGACACCGCGTCTTGCAAAGCGCA
TCGAAATGATGCTTGCAGCCGGGGCACTTGATGAAGCCCGGAACGCCATGGGTATATGTGACAATCCCCGCGCTCCCGGT
TGGTCGGGAATCGGTTGTGCCGAATTGTATTCTTATATCAAGGGAGCGGTCAGCCTTGATGAATGCAAGCGCCTGTGGTT
GCACAACACACGGCAATATGCCAAGCGCCAGCTTACCTGGTTCCGCAGAGAACCGAATCTGCACTGGCTGGACGGTACGG
ATATGGCGGCCATGGAACGGCTGGTACACTCTGTGGCAGGCAGCACCGGCTGA

Upstream 100 bases:

>100_bases
CCAGTCTGGGCGGAGATATCAAAGGACTTGTTCCGGACAATGTCTACCGCAGACTGCGTGAAAAATACGGCTACCCTTAT
CCGCTTAACGGGTAGTGCGC

Downstream 100 bases:

>100_bases
TGGGCATCTGTCTGCGTTTTCTGCTGTCCGGAGTTATTCTGCTTTTTGCCTGCTCCCTTTTGCCGCTCAAAGCTTCGGCA
GCAGCCGCTCAGGGAAGTTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSEASVIPVVCIVGPTGAGKTAASLHLAAVFDGAVINCDSRQVYKDFPVITAQPTLEEQASCPHHLYGFMGIQEKMSAGV
FLDMACPIIKEEHAHGRLPLLVGGTGLYLRALLDGIAAIPDIPETVSQKWQERCAARGAPALHRLLEAQDPQTAERLHPN
DSQRIVRALEVQEATGRPLSWWHAQPVPPSPYRAVKIGVSATLDGLTPRLAKRIEMMLAAGALDEARNAMGICDNPRAPG
WSGIGCAELYSYIKGAVSLDECKRLWLHNTRQYAKRQLTWFRREPNLHWLDGTDMAAMERLVHSVAGSTG

Sequences:

>Translated_310_residues
MSEASVIPVVCIVGPTGAGKTAASLHLAAVFDGAVINCDSRQVYKDFPVITAQPTLEEQASCPHHLYGFMGIQEKMSAGV
FLDMACPIIKEEHAHGRLPLLVGGTGLYLRALLDGIAAIPDIPETVSQKWQERCAARGAPALHRLLEAQDPQTAERLHPN
DSQRIVRALEVQEATGRPLSWWHAQPVPPSPYRAVKIGVSATLDGLTPRLAKRIEMMLAAGALDEARNAMGICDNPRAPG
WSGIGCAELYSYIKGAVSLDECKRLWLHNTRQYAKRQLTWFRREPNLHWLDGTDMAAMERLVHSVAGSTG
>Mature_309_residues
SEASVIPVVCIVGPTGAGKTAASLHLAAVFDGAVINCDSRQVYKDFPVITAQPTLEEQASCPHHLYGFMGIQEKMSAGVF
LDMACPIIKEEHAHGRLPLLVGGTGLYLRALLDGIAAIPDIPETVSQKWQERCAARGAPALHRLLEAQDPQTAERLHPND
SQRIVRALEVQEATGRPLSWWHAQPVPPSPYRAVKIGVSATLDGLTPRLAKRIEMMLAAGALDEARNAMGICDNPRAPGW
SGIGCAELYSYIKGAVSLDECKRLWLHNTRQYAKRQLTWFRREPNLHWLDGTDMAAMERLVHSVAGSTG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=27.831715210356, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1790613, Length=294, Percent_Identity=35.3741496598639, Blast_Score=174, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25144712, Length=238, Percent_Identity=27.7310924369748, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=306, Percent_Identity=30.0653594771242, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24649830, Length=364, Percent_Identity=29.6703296703297, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_DESDG (Q310R5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388276.1
- ProteinModelPortal:   Q310R5
- SMR:   Q310R5
- STRING:   Q310R5
- GeneID:   3756784
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1784
- NMPDR:   fig|207559.3.peg.1597
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   DDES207559:DDE_1784-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33810; Mature: 33679

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEASVIPVVCIVGPTGAGKTAASLHLAAVFDGAVINCDSRQVYKDFPVITAQPTLEEQA
CCCCCEEEEEEEECCCCCCCHHHHEEHHEEECCEEEECCCCHHHHCCCCEECCCCHHHHH
SCPHHLYGFMGIQEKMSAGVFLDMACPIIKEEHAHGRLPLLVGGTGLYLRALLDGIAAIP
CCCHHHHHHHHHHHHHHCCEEEEHHCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCC
DIPETVSQKWQERCAARGAPALHRLLEAQDPQTAERLHPNDSQRIVRALEVQEATGRPLS
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCC
WWHAQPVPPSPYRAVKIGVSATLDGLTPRLAKRIEMMLAAGALDEARNAMGICDNPRAPG
CCCCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
WSGIGCAELYSYIKGAVSLDECKRLWLHNTRQYAKRQLTWFRREPNLHWLDGTDMAAMER
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
LVHSVAGSTG
HHHHHHCCCC
>Mature Secondary Structure 
SEASVIPVVCIVGPTGAGKTAASLHLAAVFDGAVINCDSRQVYKDFPVITAQPTLEEQA
CCCCEEEEEEEECCCCCCCHHHHEEHHEEECCEEEECCCCHHHHCCCCEECCCCHHHHH
SCPHHLYGFMGIQEKMSAGVFLDMACPIIKEEHAHGRLPLLVGGTGLYLRALLDGIAAIP
CCCHHHHHHHHHHHHHHCCEEEEHHCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCC
DIPETVSQKWQERCAARGAPALHRLLEAQDPQTAERLHPNDSQRIVRALEVQEATGRPLS
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCC
WWHAQPVPPSPYRAVKIGVSATLDGLTPRLAKRIEMMLAAGALDEARNAMGICDNPRAPG
CCCCCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCC
WSGIGCAELYSYIKGAVSLDECKRLWLHNTRQYAKRQLTWFRREPNLHWLDGTDMAAMER
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
LVHSVAGSTG
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA