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The map label for this gene is thiE [H]

Identifier: 78356601

GI number: 78356601

Start: 1577163

End: 1577807

Strand: Direct

Name: thiE [H]

Synonym: Dde_1558

Alternate gene names: 78356601

Gene position: 1577163-1577807 (Clockwise)

Preceding gene: 78356600

Following gene: 78356602

Centisome position: 42.28

GC content: 55.04

Gene sequence:

>645_bases
ATGAAGAATAATATGGCTGACTGGGATATCTACTGTCTGACCGATGCCGGACTTTCACGCGGGCGTGATACCGTGGAGGT
TGTCCGTGCCATGCTGCAGGGCGGCGCCAGAGTGATACAATATCGTGAAAAAGACAAAAAAGCCGGTGAAATGCTGCGTG
AATGCATGGAGATTCGCAAGCTGACCAGAGATGCCGGTTGCTGCTTTATTGTCAACGATCATATTGATGTGGCCATGCTG
TGCGATGCGGACGGCGTACATGTGGGGCAGGAAGACATTCCCGTACCGGAAGTGCGTCGTCTTGTGGGCGATGGAAAAAT
CATCGGGCTTTCCACCCATTCGCCGGAGCAGTGCTGTGACGCTGTTGCACAGGGCGCTGATTATATAGGGGTGGGGCCTG
TTTTTTTCACCCGTACCAAAAAAGATGTCTGTGCGCCTGTGGGGTTTGAATACCTTGATTATGTGGCTGCAAACCACACT
GTGCCCTTTGTGGCCATAGGCGGCATCAAGGAACATAATATCGAGGAAGTGGCGGCTCACGGGGCGCGCTGCTGCGCGCT
CGTTTCTGCCATTACGGGAGCAGATGACATAGCAGCCACCGTCGCCGGTCTGCGCGGCAGGTTGAGGAGTGCCTCCGGTC
GCTGA

Upstream 100 bases:

>100_bases
CGCCGCGGGTACAGATGGCTGCCGCCATGCAGGCGGACGCAGTTCTTTGCCGGATTCTGGGCCCCTGCGTGACGGCAGAT
TGTATCAGGGAGCAGGAGTG

Downstream 100 bases:

>100_bases
TTACTGTGGTCCTTGTGTCGGCCGTGCATGCTGCGCTGCGAGGGGCCTTTCCGGTGCTCTGGTATCTGTAGCGGGTGCAG
TGCCGGCCATATTTTTTTGT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKNNMADWDIYCLTDAGLSRGRDTVEVVRAMLQGGARVIQYREKDKKAGEMLRECMEIRKLTRDAGCCFIVNDHIDVAML
CDADGVHVGQEDIPVPEVRRLVGDGKIIGLSTHSPEQCCDAVAQGADYIGVGPVFFTRTKKDVCAPVGFEYLDYVAANHT
VPFVAIGGIKEHNIEEVAAHGARCCALVSAITGADDIAATVAGLRGRLRSASGR

Sequences:

>Translated_214_residues
MKNNMADWDIYCLTDAGLSRGRDTVEVVRAMLQGGARVIQYREKDKKAGEMLRECMEIRKLTRDAGCCFIVNDHIDVAML
CDADGVHVGQEDIPVPEVRRLVGDGKIIGLSTHSPEQCCDAVAQGADYIGVGPVFFTRTKKDVCAPVGFEYLDYVAANHT
VPFVAIGGIKEHNIEEVAAHGARCCALVSAITGADDIAATVAGLRGRLRSASGR
>Mature_214_residues
MKNNMADWDIYCLTDAGLSRGRDTVEVVRAMLQGGARVIQYREKDKKAGEMLRECMEIRKLTRDAGCCFIVNDHIDVAML
CDADGVHVGQEDIPVPEVRRLVGDGKIIGLSTHSPEQCCDAVAQGADYIGVGPVFFTRTKKDVCAPVGFEYLDYVAANHT
VPFVAIGGIKEHNIEEVAAHGARCCALVSAITGADDIAATVAGLRGRLRSASGR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=190, Percent_Identity=34.7368421052632, Blast_Score=92, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325042, Length=222, Percent_Identity=37.8378378378378, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23053; Mature: 23053

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNMADWDIYCLTDAGLSRGRDTVEVVRAMLQGGARVIQYREKDKKAGEMLRECMEIRK
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHH
LTRDAGCCFIVNDHIDVAMLCDADGVHVGQEDIPVPEVRRLVGDGKIIGLSTHSPEQCCD
HHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHH
AVAQGADYIGVGPVFFTRTKKDVCAPVGFEYLDYVAANHTVPFVAIGGIKEHNIEEVAAH
HHHCCCCEEECCCEEEECCCHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
GARCCALVSAITGADDIAATVAGLRGRLRSASGR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNNMADWDIYCLTDAGLSRGRDTVEVVRAMLQGGARVIQYREKDKKAGEMLRECMEIRK
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHH
LTRDAGCCFIVNDHIDVAMLCDADGVHVGQEDIPVPEVRRLVGDGKIIGLSTHSPEQCCD
HHHCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHH
AVAQGADYIGVGPVFFTRTKKDVCAPVGFEYLDYVAANHTVPFVAIGGIKEHNIEEVAAH
HHHCCCCEEECCCEEEECCCHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
GARCCALVSAITGADDIAATVAGLRGRLRSASGR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA