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The map label for this gene is thiF [H]

Identifier: 78356600

GI number: 78356600

Start: 1576522

End: 1577163

Strand: Direct

Name: thiF [H]

Synonym: Dde_1557

Alternate gene names: 78356600

Gene position: 1576522-1577163 (Clockwise)

Preceding gene: 78356599

Following gene: 78356601

Centisome position: 42.26

GC content: 60.9

Gene sequence:

>642_bases
ATGACGGGTATGTTGCGCAGGGGGTTGGGCAGTCACCTGAAGGAGCATCAGCTTGAGCTTCTGTCAGGTGCTGTGGTGGG
GATTGCCGGTGCCGGAGGACTGGGTTCCAACTGTGCGGCCATGCTGGTGCGCAGCGGCATCCGTAATCTGGTGCTGGCCG
ATCATGACCATGTGGAACCTTCCAATCTGAACAGACAGTGGTTCACGCCGCAGCAGGTCGGGAAGTCCAAGGTGCGCGCG
CTGGCCGACAACCTGTATGCCCTGGAACCGGAACTGGTTCTGAGCGTGCATCAGGTGCGTATTGACCGCACATTACTGCA
TGATTTGTTCGGCAGTTGCTCCGTAGTGGTGGAGGCTGTGGATGATGCCGCAACCAAGAGCATGATTGTTTCCTACTGCA
TGGAGCACGGGATTTTTTTTGTAAGCGCTTCGGGCATGGGCGGCTGGGGCGGCCCCGGTATGCAGGTGCGCGGAGTGCGG
AATGCCGGCGTGGTGGTGGGAGACATGCAGACTGCCGTGGTCCCGGGGGTACCCCCTATGGCGCCGCGGGTACAGATGGC
TGCCGCCATGCAGGCGGACGCAGTTCTTTGCCGGATTCTGGGCCCCTGCGTGACGGCAGATTGTATCAGGGAGCAGGAGT
GA

Upstream 100 bases:

>100_bases
CTGACCCGCGCAGTGTGGACGAGGTGGTTGAAGCCGTAAGCCGGGCCGGTTATCAGGCCGTGTTTAAAGACTGGCATCCG
CTGGAAGACAGCTGCGCGGT

Downstream 100 bases:

>100_bases
TGAAGAATAATATGGCTGACTGGGATATCTACTGTCTGACCGATGCCGGACTTTCACGCGGGCGTGATACCGTGGAGGTT
GTCCGTGCCATGCTGCAGGG

Product: thiamine biosynthesis protein ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTGMLRRGLGSHLKEHQLELLSGAVVGIAGAGGLGSNCAAMLVRSGIRNLVLADHDHVEPSNLNRQWFTPQQVGKSKVRA
LADNLYALEPELVLSVHQVRIDRTLLHDLFGSCSVVVEAVDDAATKSMIVSYCMEHGIFFVSASGMGGWGGPGMQVRGVR
NAGVVVGDMQTAVVPGVPPMAPRVQMAAAMQADAVLCRILGPCVTADCIREQE

Sequences:

>Translated_213_residues
MTGMLRRGLGSHLKEHQLELLSGAVVGIAGAGGLGSNCAAMLVRSGIRNLVLADHDHVEPSNLNRQWFTPQQVGKSKVRA
LADNLYALEPELVLSVHQVRIDRTLLHDLFGSCSVVVEAVDDAATKSMIVSYCMEHGIFFVSASGMGGWGGPGMQVRGVR
NAGVVVGDMQTAVVPGVPPMAPRVQMAAAMQADAVLCRILGPCVTADCIREQE
>Mature_212_residues
TGMLRRGLGSHLKEHQLELLSGAVVGIAGAGGLGSNCAAMLVRSGIRNLVLADHDHVEPSNLNRQWFTPQQVGKSKVRAL
ADNLYALEPELVLSVHQVRIDRTLLHDLFGSCSVVVEAVDDAATKSMIVSYCMEHGIFFVSASGMGGWGGPGMQVRGVRN
AGVVVGDMQTAVVPGVPPMAPRVQMAAAMQADAVLCRILGPCVTADCIREQE

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Escherichia coli, GI87082356, Length=141, Percent_Identity=34.7517730496454, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1789177, Length=141, Percent_Identity=31.9148936170213, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=132, Percent_Identity=37.1212121212121, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 22541; Mature: 22409

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGMLRRGLGSHLKEHQLELLSGAVVGIAGAGGLGSNCAAMLVRSGIRNLVLADHDHVEP
CCCHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCC
SNLNRQWFTPQQVGKSKVRALADNLYALEPELVLSVHQVRIDRTLLHDLFGSCSVVVEAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDAATKSMIVSYCMEHGIFFVSASGMGGWGGPGMQVRGVRNAGVVVGDMQTAVVPGVPPM
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEECCCCCEEEECCHHHCCCCCCCC
APRVQMAAAMQADAVLCRILGPCVTADCIREQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGMLRRGLGSHLKEHQLELLSGAVVGIAGAGGLGSNCAAMLVRSGIRNLVLADHDHVEP
CCHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCCC
SNLNRQWFTPQQVGKSKVRALADNLYALEPELVLSVHQVRIDRTLLHDLFGSCSVVVEAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDAATKSMIVSYCMEHGIFFVSASGMGGWGGPGMQVRGVRNAGVVVGDMQTAVVPGVPPM
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEECCCCCEEEECCHHHCCCCCCCC
APRVQMAAAMQADAVLCRILGPCVTADCIREQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]