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The map label for this gene is thiH

Identifier: 78356599

GI number: 78356599

Start: 1575356

End: 1576525

Strand: Direct

Name: thiH

Synonym: Dde_1556

Alternate gene names: 78356599

Gene position: 1575356-1576525 (Clockwise)

Preceding gene: 78356598

Following gene: 78356600

Centisome position: 42.23

GC content: 57.26

Gene sequence:

>1170_bases
ATGAGTGAAATCAGCGCTGACATGAGTGTGAGAACCGCACAGGCGGGAGGTTTTTACGAAGTTGTCCGCCAGTTGCAGGA
TGTGGATGTGCAGGCCGCCTGCATGGCGGCTGACGGATACAGAGTGCGGCAGGCTCTGGCAAAGGATACCCTTTCTCCGG
AAGACTTTCTGGCTTTGCTTTCACCCGCGGCCCGTCCGTACATGGAATCAATGGCGCATAAGGCCCGTGATGTCACGGTG
CGCCAGTTCGGCCGCACCATCCAGCTGTTTACTCCGCTGTACCTTTCAAACTGGTGTACAAACAGGTGCGTGTATTGCAG
CTTCAATGCCTGCAGTGGCATAGACCGCATGCAGCTGGATGCTGCGGGTGTGCTGCGCGAAGGGCAGGCCATAGCCGCCA
CAGGACTGCGCCATCTGCTGCTGCTGACCGGCGAGGCTCCGGCAAAGGCTTCCGTTGACTACATCCGCGACTGTGTGCGC
GTGTTGCGTCCTCTGTTTCCGTCACTGAGTATTGAAGTGTACGCCCTGACCGAGCCTGAGTACCGCACACTTGCCGTTGC
CGGTGTGGACGGCATGACACTTTTTCAGGAAACATATAACGAGGCTTTGTACCCGTCATTGCATCCTGCCGGTCCCAAAA
GCAATTATCATTTCAGGCTGGGGGCGCCGGAAAGAGCATGCCGTGCCGGAATGCGCAATGTGAATATAGGTGCGTTGCTC
GGACTGGATCAGTGGCAGCGTGATGCTTTTATGACGGGTATGCATGCCCTTTGGCTGCAGCACAGGTATCCGGGAGTGGA
TATTGCTGTTTCGCTGCCGCGTATGCGTCCTTATGCCGGCAGTTTTCAGCCGGTGTGTGACGTGGATGATCGTGCGCTGG
TGCAGATACTGCTGGCCATGCGGCTTTTTCTGCCGCGGTGCGGCATTACCATTTCCACGCGCGAACGGCCTGCATTCCGC
GATAATCTGATACCGCTGGGAGTGACCCGCATGAGTGCCGGTGTATCCACCGCTGTGGGCGGACATGCTGAAAACGAACA
TTCCTCTGTAGGGCAGTTTGAAATTTCTGACCCGCGCAGTGTGGACGAGGTGGTTGAAGCCGTAAGCCGGGCCGGTTATC
AGGCCGTGTTTAAAGACTGGCATCCGCTGGAAGACAGCTGCGCGGTATGA

Upstream 100 bases:

>100_bases
GAACAGGCGCCGTGCTGGAAGGGCAGGCGCAGGCTTCTTCGCCTTTGCTGGGCTTTCTGGATTCATGATGTGCAGCCATG
AGAAAATGACGGGGAGCGTG

Downstream 100 bases:

>100_bases
CGGGTATGTTGCGCAGGGGGTTGGGCAGTCACCTGAAGGAGCATCAGCTTGAGCTTCTGTCAGGTGCTGTGGTGGGGATT
GCCGGTGCCGGAGGACTGGG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSEISADMSVRTAQAGGFYEVVRQLQDVDVQAACMAADGYRVRQALAKDTLSPEDFLALLSPAARPYMESMAHKARDVTV
RQFGRTIQLFTPLYLSNWCTNRCVYCSFNACSGIDRMQLDAAGVLREGQAIAATGLRHLLLLTGEAPAKASVDYIRDCVR
VLRPLFPSLSIEVYALTEPEYRTLAVAGVDGMTLFQETYNEALYPSLHPAGPKSNYHFRLGAPERACRAGMRNVNIGALL
GLDQWQRDAFMTGMHALWLQHRYPGVDIAVSLPRMRPYAGSFQPVCDVDDRALVQILLAMRLFLPRCGITISTRERPAFR
DNLIPLGVTRMSAGVSTAVGGHAENEHSSVGQFEISDPRSVDEVVEAVSRAGYQAVFKDWHPLEDSCAV

Sequences:

>Translated_389_residues
MSEISADMSVRTAQAGGFYEVVRQLQDVDVQAACMAADGYRVRQALAKDTLSPEDFLALLSPAARPYMESMAHKARDVTV
RQFGRTIQLFTPLYLSNWCTNRCVYCSFNACSGIDRMQLDAAGVLREGQAIAATGLRHLLLLTGEAPAKASVDYIRDCVR
VLRPLFPSLSIEVYALTEPEYRTLAVAGVDGMTLFQETYNEALYPSLHPAGPKSNYHFRLGAPERACRAGMRNVNIGALL
GLDQWQRDAFMTGMHALWLQHRYPGVDIAVSLPRMRPYAGSFQPVCDVDDRALVQILLAMRLFLPRCGITISTRERPAFR
DNLIPLGVTRMSAGVSTAVGGHAENEHSSVGQFEISDPRSVDEVVEAVSRAGYQAVFKDWHPLEDSCAV
>Mature_388_residues
SEISADMSVRTAQAGGFYEVVRQLQDVDVQAACMAADGYRVRQALAKDTLSPEDFLALLSPAARPYMESMAHKARDVTVR
QFGRTIQLFTPLYLSNWCTNRCVYCSFNACSGIDRMQLDAAGVLREGQAIAATGLRHLLLLTGEAPAKASVDYIRDCVRV
LRPLFPSLSIEVYALTEPEYRTLAVAGVDGMTLFQETYNEALYPSLHPAGPKSNYHFRLGAPERACRAGMRNVNIGALLG
LDQWQRDAFMTGMHALWLQHRYPGVDIAVSLPRMRPYAGSFQPVCDVDDRALVQILLAMRLFLPRCGITISTRERPAFRD
NLIPLGVTRMSAGVSTAVGGHAENEHSSVGQFEISDPRSVDEVVEAVSRAGYQAVFKDWHPLEDSCAV

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=364, Percent_Identity=42.3076923076923, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42925; Mature: 42793

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEISADMSVRTAQAGGFYEVVRQLQDVDVQAACMAADGYRVRQALAKDTLSPEDFLALL
CCCCCCCCEEEECCCCCHHHHHHHHHCCCHHHEEEECCCHHHHHHHHHCCCCHHHHHHHH
SPAARPYMESMAHKARDVTVRQFGRTIQLFTPLYLSNWCTNRCVYCSFNACSGIDRMQLD
HHCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCEEEEECHHHCCCCHHHHH
AAGVLREGQAIAATGLRHLLLLTGEAPAKASVDYIRDCVRVLRPLFPSLSIEVYALTEPE
HHHHHHCCCHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
YRTLAVAGVDGMTLFQETYNEALYPSLHPAGPKSNYHFRLGAPERACRAGMRNVNIGALL
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCEEE
GLDQWQRDAFMTGMHALWLQHRYPGVDIAVSLPRMRPYAGSFQPVCDVDDRALVQILLAM
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLFLPRCGITISTRERPAFRDNLIPLGVTRMSAGVSTAVGGHAENEHSSVGQFEISDPRS
HHHHHHCCCEEECCCCCCHHCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCEEECCCCCC
VDEVVEAVSRAGYQAVFKDWHPLEDSCAV
HHHHHHHHHHCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEISADMSVRTAQAGGFYEVVRQLQDVDVQAACMAADGYRVRQALAKDTLSPEDFLALL
CCCCCCCEEEECCCCCHHHHHHHHHCCCHHHEEEECCCHHHHHHHHHCCCCHHHHHHHH
SPAARPYMESMAHKARDVTVRQFGRTIQLFTPLYLSNWCTNRCVYCSFNACSGIDRMQLD
HHCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCEEEEECHHHCCCCHHHHH
AAGVLREGQAIAATGLRHLLLLTGEAPAKASVDYIRDCVRVLRPLFPSLSIEVYALTEPE
HHHHHHCCCHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
YRTLAVAGVDGMTLFQETYNEALYPSLHPAGPKSNYHFRLGAPERACRAGMRNVNIGALL
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCEEE
GLDQWQRDAFMTGMHALWLQHRYPGVDIAVSLPRMRPYAGSFQPVCDVDDRALVQILLAM
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RLFLPRCGITISTRERPAFRDNLIPLGVTRMSAGVSTAVGGHAENEHSSVGQFEISDPRS
HHHHHHCCCEEECCCCCCHHCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCEEECCCCCC
VDEVVEAVSRAGYQAVFKDWHPLEDSCAV
HHHHHHHHHHCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]