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The map label for this gene is thiG

Identifier: 78356598

GI number: 78356598

Start: 1574529

End: 1575323

Strand: Direct

Name: thiG

Synonym: Dde_1555

Alternate gene names: 78356598

Gene position: 1574529-1575323 (Clockwise)

Preceding gene: 78356597

Following gene: 78356599

Centisome position: 42.21

GC content: 59.62

Gene sequence:

>795_bases
ATGGAACACACAACAACACACAACAACGATCCACTGGTACTTGGCGGACGCGCTCTGGAAAGTCGTCTTTTCATAGGCAC
CGGCAAGTACGGCTCCGACAGTCTCATACCCCGGGTGGCAGAAGCCAGCGGCGCACAGGTGATTACGGTGGCATTGCGGC
GTGTGGACATGCAGGCTGCCGCAGGCAACGTCATGCAGCACATTCCGCAGCATATGCAGCTTTTACCCAACACATCCGGG
GCGCGCACGGCTGAAGAAGCCGTGCGCATCGCCCGGCTGGCCCGCGCCGCAGGATGCGGCGACTGGATTAAAATCGAGGT
GATTTCGGACAGCAGGTATCTGCTGCCGGACGGGTATGAAACTGCAAAAGCCACTGAGATACTGGCACGGGACGGTTTTG
TGGTTTTGCCGTACATGAATCCCGACCTGTATGTGGCGCGCGATCTTGTCAGTGCGGGAGCCGCTGCGGTCATGCCTCTG
GGGGCCCCCATAGGAACCAACAGAGGGCTGCGCACGCAGGAGATGATAGGTATTCTTATTGAAGAGATTGAACTGCCCGT
CATTGTCGATGCCGGAATAGGGCGTCCTTCGCAGGCCTGCGAGGCAATGGAAATGGGCGCTGCAGCCTGTCTTGTCAATA
CGGCCATAGCTTCTGCGGGCGATCCTGTTCTTATGGCATCGGCATTCGGCGCTGCAGTGCGGGCCGGGCGCAGAGCCTGG
CTGGCCGGAACAGGCGCCGTGCTGGAAGGGCAGGCGCAGGCTTCTTCGCCTTTGCTGGGCTTTCTGGATTCATGA

Upstream 100 bases:

>100_bases
TGGAATGCAACCGCGCCATCATACCGCCGGACGCATTTGCGGCAACGGTGCTGCACGAAGGGGATGAACTGGAAATCCTG
CACTTTGTAGGAGGGGGCTA

Downstream 100 bases:

>100_bases
TGTGCAGCCATGAGAAAATGACGGGGAGCGTGATGAGTGAAATCAGCGCTGACATGAGTGTGAGAACCGCACAGGCGGGA
GGTTTTTACGAAGTTGTCCG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MEHTTTHNNDPLVLGGRALESRLFIGTGKYGSDSLIPRVAEASGAQVITVALRRVDMQAAAGNVMQHIPQHMQLLPNTSG
ARTAEEAVRIARLARAAGCGDWIKIEVISDSRYLLPDGYETAKATEILARDGFVVLPYMNPDLYVARDLVSAGAAAVMPL
GAPIGTNRGLRTQEMIGILIEEIELPVIVDAGIGRPSQACEAMEMGAAACLVNTAIASAGDPVLMASAFGAAVRAGRRAW
LAGTGAVLEGQAQASSPLLGFLDS

Sequences:

>Translated_264_residues
MEHTTTHNNDPLVLGGRALESRLFIGTGKYGSDSLIPRVAEASGAQVITVALRRVDMQAAAGNVMQHIPQHMQLLPNTSG
ARTAEEAVRIARLARAAGCGDWIKIEVISDSRYLLPDGYETAKATEILARDGFVVLPYMNPDLYVARDLVSAGAAAVMPL
GAPIGTNRGLRTQEMIGILIEEIELPVIVDAGIGRPSQACEAMEMGAAACLVNTAIASAGDPVLMASAFGAAVRAGRRAW
LAGTGAVLEGQAQASSPLLGFLDS
>Mature_264_residues
MEHTTTHNNDPLVLGGRALESRLFIGTGKYGSDSLIPRVAEASGAQVITVALRRVDMQAAAGNVMQHIPQHMQLLPNTSG
ARTAEEAVRIARLARAAGCGDWIKIEVISDSRYLLPDGYETAKATEILARDGFVVLPYMNPDLYVARDLVSAGAAAVMPL
GAPIGTNRGLRTQEMIGILIEEIELPVIVDAGIGRPSQACEAMEMGAAACLVNTAIASAGDPVLMASAFGAAVRAGRRAW
LAGTGAVLEGQAQASSPLLGFLDS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=50.3937007874016, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_DESDG (Q311P4)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388047.1
- ProteinModelPortal:   Q311P4
- SMR:   Q311P4
- STRING:   Q311P4
- GeneID:   3755994
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1555
- NMPDR:   fig|207559.3.peg.959
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   DDES207559:DDE_1555-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27573; Mature: 27573

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: ACT_SITE 104-104 BINDING 165-165

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHTTTHNNDPLVLGGRALESRLFIGTGKYGSDSLIPRVAEASGAQVITVALRRVDMQAA
CCCCCCCCCCCEEEECHHHCCEEEEECCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHH
AGNVMQHIPQHMQLLPNTSGARTAEEAVRIARLARAAGCGDWIKIEVISDSRYLLPDGYE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCCC
TAKATEILARDGFVVLPYMNPDLYVARDLVSAGAAAVMPLGAPIGTNRGLRTQEMIGILI
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHH
EEIELPVIVDAGIGRPSQACEAMEMGAAACLVNTAIASAGDPVLMASAFGAAVRAGRRAW
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCEEE
LAGTGAVLEGQAQASSPLLGFLDS
EEECCCEEECCCCCCCCCEEECCC
>Mature Secondary Structure
MEHTTTHNNDPLVLGGRALESRLFIGTGKYGSDSLIPRVAEASGAQVITVALRRVDMQAA
CCCCCCCCCCCEEEECHHHCCEEEEECCCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHHH
AGNVMQHIPQHMQLLPNTSGARTAEEAVRIARLARAAGCGDWIKIEVISDSRYLLPDGYE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCCC
TAKATEILARDGFVVLPYMNPDLYVARDLVSAGAAAVMPLGAPIGTNRGLRTQEMIGILI
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHH
EEIELPVIVDAGIGRPSQACEAMEMGAAACLVNTAIASAGDPVLMASAFGAAVRAGRRAW
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCEEE
LAGTGAVLEGQAQASSPLLGFLDS
EEECCCEEECCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA