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The map label for this gene is dppA [H]

Identifier: 78356232

GI number: 78356232

Start: 1214202

End: 1215827

Strand: Reverse

Name: dppA [H]

Synonym: Dde_1185

Alternate gene names: 78356232

Gene position: 1215827-1214202 (Counterclockwise)

Preceding gene: 78356233

Following gene: 78356231

Centisome position: 32.59

GC content: 56.03

Gene sequence:

>1626_bases
ATGCTGAAAAAACTCTTGCTGTTCACGCTGGCTGCGGTTGCACTGACAGCCTATTCGCTCACCCCCCAGTCTGTTCTGGC
GGCGCCCAAACAGGGCGGAACGCTGGTCTTTGCCCGCGGTGCAGACAGCCCCGGTCTTGACCCCGCATATGAGACTGACG
GCAACTCGTTCATGGTGTGCGACAACATCTACGACGCACTTGTGGGCTTTATGCCCGAATCCACTGCGCTTACCCCCTCG
CTGGCCACATCGTGGGATATTTCCGAAGACGGCATGACCTATACCTTCCATCTGCGCGAAGGTGTCAGCTTCCACGACGG
CACCCCCTTCAATGCCGATGCCGTTGTCTTTTCACACGGACGCCAGATGAAAGACAAAAGCAAAGCCAAATTTTTCAGCA
AGCAGTGGGGCTGGCCCGAACAGGCTCCCGCTGCGGAATACTGGCTTTCCATGGAAATGGACGACATCATCGAATCCATT
GAAGCAAAAGACGACTACACCGTGGTGTACAAACTCAAGCGCCCGGAAGCTCCTTTTCTTGCCAACATGGCCATGGACTT
TGCCGACATAGTCAGCCCCACCGCCGTTCTCAAGCATGGTGCCGATTACATAACCAACCCCGTGGGCACCGGTCCTTTCA
AGTTTGTCTCGTGGCAGAAAAACGACCGCATCACCCTTGAACGCAACGACGACTACTGGGACGGCGCCCCCTACCTCGAT
AAAGTCCTCTTCCGCGTCATTCCCGACAACTCGGTCCGTTTTCTCGAACTGATGTCCGGCAATGTGCACATCTGCCAGTA
CCCCAACCCCGAAGACGTGGAACTGGCAAAAAGCAACGGCAACCTTGAAGTGATCACCCAGCCCGGCATGAACGTGGGCT
ACCTGTCATTCAACCACACCAAGGAACTCTGGCAGGATGCGCGCATCCGCCGTGCCATCGCCCATGCAGTCAACAAGCAG
GCCATAGTGGACAACATCTACTACGGCATGGGTGTTCCCGCGGTGAACACCATTCCTCCCACCATGTGGGGCTACAACAA
TGACATCACCGACTACGAATACAACCCCGAAAAAGCCCGCCAGCTGCTGAAGGAAGCCGATTTTGAAGGCAAACTGAAAG
CCGCCGGACAGGACAGGATAACCCTGTGGTGCATGCCTGTGGCACGTCCCTACAATCCCAGCGGCATGAAAGTGGGTGAA
GCCATTCAGGCCGACCTGAAAGCCGTGGGCATAAACGTGGAACTGGTGACCATGGAATGGGGAACCTACCTCAAAAAGCA
GCGTTCGCAGGATCCGGGCATGGACCTGTTCCAGCTGGGCTGGACCGGTGACAACGGCGACCCCGACAACTTCCTTGCCA
TCCTGCTGGACGGTATGGCCGACCCCAACGTGCGCACCCAGTGGAAAAACGAAGAATATCACCAGCTGATGGTGGACGGA
AAGCGCACCACGGATCAGGCCAAGCGCATTGAAATCTACAAAAAGGCGCAGGAACTTATCCATCAGGAAGCTCCCATGAT
CAACATGGCGCATTCCATCGTGGCACAGCCCCAGCACAAGCGCGTGCAGGACTTTAAACTGCACCCGACAACCAGTGTAT
ACCTGCACAAGGTATGGCTGAAGTAG

Upstream 100 bases:

>100_bases
GTGATATAATTAACCGGATGACGCCACCCCGCCGTCTGCCCGGCAGGCGGCGGGGAACCATACGGCAGAACACAACCATC
TTTCTTTCCGGGAGGCTTGC

Downstream 100 bases:

>100_bases
CCCCACAGGCAGACCACGACATGTGAACCATCTACCGGCAGGGGCGCGCAACCGCCCCTGCCGTTGTATTCAACGGGAAG
AGCATTGCTACATTTTCTGA

Product: putative oligopeptide ABC transporter binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MLKKLLLFTLAAVALTAYSLTPQSVLAAPKQGGTLVFARGADSPGLDPAYETDGNSFMVCDNIYDALVGFMPESTALTPS
LATSWDISEDGMTYTFHLREGVSFHDGTPFNADAVVFSHGRQMKDKSKAKFFSKQWGWPEQAPAAEYWLSMEMDDIIESI
EAKDDYTVVYKLKRPEAPFLANMAMDFADIVSPTAVLKHGADYITNPVGTGPFKFVSWQKNDRITLERNDDYWDGAPYLD
KVLFRVIPDNSVRFLELMSGNVHICQYPNPEDVELAKSNGNLEVITQPGMNVGYLSFNHTKELWQDARIRRAIAHAVNKQ
AIVDNIYYGMGVPAVNTIPPTMWGYNNDITDYEYNPEKARQLLKEADFEGKLKAAGQDRITLWCMPVARPYNPSGMKVGE
AIQADLKAVGINVELVTMEWGTYLKKQRSQDPGMDLFQLGWTGDNGDPDNFLAILLDGMADPNVRTQWKNEEYHQLMVDG
KRTTDQAKRIEIYKKAQELIHQEAPMINMAHSIVAQPQHKRVQDFKLHPTTSVYLHKVWLK

Sequences:

>Translated_541_residues
MLKKLLLFTLAAVALTAYSLTPQSVLAAPKQGGTLVFARGADSPGLDPAYETDGNSFMVCDNIYDALVGFMPESTALTPS
LATSWDISEDGMTYTFHLREGVSFHDGTPFNADAVVFSHGRQMKDKSKAKFFSKQWGWPEQAPAAEYWLSMEMDDIIESI
EAKDDYTVVYKLKRPEAPFLANMAMDFADIVSPTAVLKHGADYITNPVGTGPFKFVSWQKNDRITLERNDDYWDGAPYLD
KVLFRVIPDNSVRFLELMSGNVHICQYPNPEDVELAKSNGNLEVITQPGMNVGYLSFNHTKELWQDARIRRAIAHAVNKQ
AIVDNIYYGMGVPAVNTIPPTMWGYNNDITDYEYNPEKARQLLKEADFEGKLKAAGQDRITLWCMPVARPYNPSGMKVGE
AIQADLKAVGINVELVTMEWGTYLKKQRSQDPGMDLFQLGWTGDNGDPDNFLAILLDGMADPNVRTQWKNEEYHQLMVDG
KRTTDQAKRIEIYKKAQELIHQEAPMINMAHSIVAQPQHKRVQDFKLHPTTSVYLHKVWLK
>Mature_541_residues
MLKKLLLFTLAAVALTAYSLTPQSVLAAPKQGGTLVFARGADSPGLDPAYETDGNSFMVCDNIYDALVGFMPESTALTPS
LATSWDISEDGMTYTFHLREGVSFHDGTPFNADAVVFSHGRQMKDKSKAKFFSKQWGWPEQAPAAEYWLSMEMDDIIESI
EAKDDYTVVYKLKRPEAPFLANMAMDFADIVSPTAVLKHGADYITNPVGTGPFKFVSWQKNDRITLERNDDYWDGAPYLD
KVLFRVIPDNSVRFLELMSGNVHICQYPNPEDVELAKSNGNLEVITQPGMNVGYLSFNHTKELWQDARIRRAIAHAVNKQ
AIVDNIYYGMGVPAVNTIPPTMWGYNNDITDYEYNPEKARQLLKEADFEGKLKAAGQDRITLWCMPVARPYNPSGMKVGE
AIQADLKAVGINVELVTMEWGTYLKKQRSQDPGMDLFQLGWTGDNGDPDNFLAILLDGMADPNVRTQWKNEEYHQLMVDG
KRTTDQAKRIEIYKKAQELIHQEAPMINMAHSIVAQPQHKRVQDFKLHPTTSVYLHKVWLK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=553, Percent_Identity=40.6871609403255, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1787551, Length=541, Percent_Identity=28.8354898336414, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1787052, Length=505, Percent_Identity=28.9108910891089, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787762, Length=542, Percent_Identity=28.0442804428044, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1789397, Length=552, Percent_Identity=27.8985507246377, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1789887, Length=544, Percent_Identity=25.5514705882353, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1787495, Length=534, Percent_Identity=25.4681647940075, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI87081878, Length=509, Percent_Identity=24.1650294695481, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60983; Mature: 60983

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLLLFTLAAVALTAYSLTPQSVLAAPKQGGTLVFARGADSPGLDPAYETDGNSFMVC
CHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCEECCCCEEEEE
DNIYDALVGFMPESTALTPSLATSWDISEDGMTYTFHLREGVSFHDGTPFNADAVVFSHG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECC
RQMKDKSKAKFFSKQWGWPEQAPAAEYWLSMEMDDIIESIEAKDDYTVVYKLKRPEAPFL
CCCHHHHHHHHHHHHCCCCCCCCHHHHEEECCHHHHHHHHCCCCCEEEEEEECCCCCCHH
ANMAMDFADIVSPTAVLKHGADYITNPVGTGPFKFVSWQKNDRITLERNDDYWDGAPYLD
HHHHHHHHHHHCHHHHHHCCCHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHH
KVLFRVIPDNSVRFLELMSGNVHICQYPNPEDVELAKSNGNLEVITQPGMNVGYLSFNHT
HHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCEEEECCCCEEEEECCCCEEEEEEECCH
KELWQDARIRRAIAHAVNKQAIVDNIYYGMGVPAVNTIPPTMWGYNNDITDYEYNPEKAR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QLLKEADFEGKLKAAGQDRITLWCMPVARPYNPSGMKVGEAIQADLKAVGINVELVTMEW
HHHHHCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHEEECEEEEEEECH
GTYLKKQRSQDPGMDLFQLGWTGDNGDPDNFLAILLDGMADPNVRTQWKNEEYHQLMVDG
HHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHEEEECC
KRTTDQAKRIEIYKKAQELIHQEAPMINMAHSIVAQPQHKRVQDFKLHPTTSVYLHKVWL
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHEECCCCCEEEEEEEEC
K
C
>Mature Secondary Structure
MLKKLLLFTLAAVALTAYSLTPQSVLAAPKQGGTLVFARGADSPGLDPAYETDGNSFMVC
CHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCCCCCCEECCCCEEEEE
DNIYDALVGFMPESTALTPSLATSWDISEDGMTYTFHLREGVSFHDGTPFNADAVVFSHG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECC
RQMKDKSKAKFFSKQWGWPEQAPAAEYWLSMEMDDIIESIEAKDDYTVVYKLKRPEAPFL
CCCHHHHHHHHHHHHCCCCCCCCHHHHEEECCHHHHHHHHCCCCCEEEEEEECCCCCCHH
ANMAMDFADIVSPTAVLKHGADYITNPVGTGPFKFVSWQKNDRITLERNDDYWDGAPYLD
HHHHHHHHHHHCHHHHHHCCCHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHH
KVLFRVIPDNSVRFLELMSGNVHICQYPNPEDVELAKSNGNLEVITQPGMNVGYLSFNHT
HHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCEEEECCCCEEEEECCCCEEEEEEECCH
KELWQDARIRRAIAHAVNKQAIVDNIYYGMGVPAVNTIPPTMWGYNNDITDYEYNPEKAR
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QLLKEADFEGKLKAAGQDRITLWCMPVARPYNPSGMKVGEAIQADLKAVGINVELVTMEW
HHHHHCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHEEECEEEEEEECH
GTYLKKQRSQDPGMDLFQLGWTGDNGDPDNFLAILLDGMADPNVRTQWKNEEYHQLMVDG
HHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHEEEECC
KRTTDQAKRIEIYKKAQELIHQEAPMINMAHSIVAQPQHKRVQDFKLHPTTSVYLHKVWL
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHEECCCCCEEEEEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]