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The map label for this gene is dapE [H]

Identifier: 78356233

GI number: 78356233

Start: 1215977

End: 1217068

Strand: Reverse

Name: dapE [H]

Synonym: Dde_1186

Alternate gene names: 78356233

Gene position: 1217068-1215977 (Counterclockwise)

Preceding gene: 78356234

Following gene: 78356232

Centisome position: 32.63

GC content: 58.61

Gene sequence:

>1092_bases
GTGTCTTCTATGCAACACATTGTCGAACTGACAAGCGATCTCATCCGCTTTCCCAGCATGCACTCCAGACCGGAGCAAAT
CTCACGCTGTGCCGGTTTCATCATGGACTGGTGCGCACAGAACGGCATTCATGCCGAACGCATGGACCACGACGGAATTC
CCTCCGTTATGGTGCTGCCCGAAAAAGGCAGGGCGGGGCTGCTGCTGATGGCCCACATAGATGTGGTGGACGCGGAGGAC
GACCTGTTTGTTCCGCGTGTGGAAAATGACAGATTGTACGGACGCGGCGCCAATGACGACAAGTATGCCGTGGCGCTGGG
ACTTGTCATGTTCAGAGACCGGCTGAACGCCCTGAAGGCCGCCGGACGCAGCCAGAAGGACATGGCGCTGGGGCTGCTTA
TCACCGGCGACGAAGAAATAGGCGGCATGAACGGCGCCGCCAAAGCCCTGCCGCTCATCAGGGCCGACTATGTGGTGGCG
CTGGACGGCGGTAATCCGCAGCAGGTCATCACCAAAGAAAAAGGCATCATCGACATAAAGCTGACCTGCACGGGAAAGGC
GGCCCATGGCGCCCGCCCGTGGATGGGAGTGAACGCCGTTGACCTGCTGATGGAGGACTATACGAGGCTGAAAACGCTGT
TTGCCGAAGAAAATGAGGACCACTGGCACCGCACGGTCAATCTGGGCAGAATACGGGCCGGAGAATCCACCAACAAGGTG
CCCGACGTGGCGGAAGGCTGGTTCAACATCCGCGTGACGGAACATGACGATCCCGGAGCACTGATTGACAAAATACGTAA
AACAGTCTCGGGAACGGTCAGCATTGTGCGCACCGTGCCTGTTTTTCTGGCTGCAGACAGCCCCTACACAGAAAGACTGC
TGGCGTTGTCCGGTGCAACGGCGGGCAAGGCCCACGGAGCAAGTGATGCCCGCTATCTGGGCGAAAACGGCCTCACGGGC
GTTGTATGGGGTGCAGAAGGCTTTAACACCCTGCATTCACGCGATGAGTGTCTGCACATTCCCAGTCTGCAGAGCATCTA
CGACCCGCTGATGCAGCTCGCCCGCGAAATGGAGGAGCATGCCGCAGTTTGA

Upstream 100 bases:

>100_bases
ACATACACGGCGCAGTGCGCGCGGGACTTGCCAAAAGCCCGTCTGCGCGCGCAAGATAGCCGTCACCTGTCACATATATC
ATATAAAAAAGGAGTCCGCA

Downstream 100 bases:

>100_bases
GTGTTCAGCTAAGGGGTTAGACAGCGGCGCTTTCCTTCGTCACATTTTTGTGATATAATTAACCGGATGACGCCACCCCG
CCGTCTGCCCGGCAGGCGGC

Product: M20/M25/M40 family peptidase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSSMQHIVELTSDLIRFPSMHSRPEQISRCAGFIMDWCAQNGIHAERMDHDGIPSVMVLPEKGRAGLLLMAHIDVVDAED
DLFVPRVENDRLYGRGANDDKYAVALGLVMFRDRLNALKAAGRSQKDMALGLLITGDEEIGGMNGAAKALPLIRADYVVA
LDGGNPQQVITKEKGIIDIKLTCTGKAAHGARPWMGVNAVDLLMEDYTRLKTLFAEENEDHWHRTVNLGRIRAGESTNKV
PDVAEGWFNIRVTEHDDPGALIDKIRKTVSGTVSIVRTVPVFLAADSPYTERLLALSGATAGKAHGASDARYLGENGLTG
VVWGAEGFNTLHSRDECLHIPSLQSIYDPLMQLAREMEEHAAV

Sequences:

>Translated_363_residues
MSSMQHIVELTSDLIRFPSMHSRPEQISRCAGFIMDWCAQNGIHAERMDHDGIPSVMVLPEKGRAGLLLMAHIDVVDAED
DLFVPRVENDRLYGRGANDDKYAVALGLVMFRDRLNALKAAGRSQKDMALGLLITGDEEIGGMNGAAKALPLIRADYVVA
LDGGNPQQVITKEKGIIDIKLTCTGKAAHGARPWMGVNAVDLLMEDYTRLKTLFAEENEDHWHRTVNLGRIRAGESTNKV
PDVAEGWFNIRVTEHDDPGALIDKIRKTVSGTVSIVRTVPVFLAADSPYTERLLALSGATAGKAHGASDARYLGENGLTG
VVWGAEGFNTLHSRDECLHIPSLQSIYDPLMQLAREMEEHAAV
>Mature_362_residues
SSMQHIVELTSDLIRFPSMHSRPEQISRCAGFIMDWCAQNGIHAERMDHDGIPSVMVLPEKGRAGLLLMAHIDVVDAEDD
LFVPRVENDRLYGRGANDDKYAVALGLVMFRDRLNALKAAGRSQKDMALGLLITGDEEIGGMNGAAKALPLIRADYVVAL
DGGNPQQVITKEKGIIDIKLTCTGKAAHGARPWMGVNAVDLLMEDYTRLKTLFAEENEDHWHRTVNLGRIRAGESTNKVP
DVAEGWFNIRVTEHDDPGALIDKIRKTVSGTVSIVRTVPVFLAADSPYTERLLALSGATAGKAHGASDARYLGENGLTGV
VWGAEGFNTLHSRDECLHIPSLQSIYDPLMQLAREMEEHAAV

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=385, Percent_Identity=27.5324675324675, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790395, Length=371, Percent_Identity=24.2587601078167, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 39715; Mature: 39584

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSMQHIVELTSDLIRFPSMHSRPEQISRCAGFIMDWCAQNGIHAERMDHDGIPSVMVLP
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEE
EKGRAGLLLMAHIDVVDAEDDLFVPRVENDRLYGRGANDDKYAVALGLVMFRDRLNALKA
CCCCCCEEEEEEEEEECCCCCEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
AGRSQKDMALGLLITGDEEIGGMNGAAKALPLIRADYVVALDGGNPQQVITKEKGIIDIK
CCCCCCCEEEEEEEECCHHHCCCCCHHHHHHEEEECEEEEECCCCCHHHHCCCCCEEEEE
LTCTGKAAHGARPWMGVNAVDLLMEDYTRLKTLFAEENEDHWHRTVNLGRIRAGESTNKV
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECEEECCCCCCCC
PDVAEGWFNIRVTEHDDPGALIDKIRKTVSGTVSIVRTVPVFLAADSPYTERLLALSGAT
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCC
AGKAHGASDARYLGENGLTGVVWGAEGFNTLHSRDECLHIPSLQSIYDPLMQLAREMEEH
CCCCCCCCCHHHHCCCCCEEEEECCCCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHH
AAV
CCC
>Mature Secondary Structure 
SSMQHIVELTSDLIRFPSMHSRPEQISRCAGFIMDWCAQNGIHAERMDHDGIPSVMVLP
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEEE
EKGRAGLLLMAHIDVVDAEDDLFVPRVENDRLYGRGANDDKYAVALGLVMFRDRLNALKA
CCCCCCEEEEEEEEEECCCCCEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
AGRSQKDMALGLLITGDEEIGGMNGAAKALPLIRADYVVALDGGNPQQVITKEKGIIDIK
CCCCCCCEEEEEEEECCHHHCCCCCHHHHHHEEEECEEEEECCCCCHHHHCCCCCEEEEE
LTCTGKAAHGARPWMGVNAVDLLMEDYTRLKTLFAEENEDHWHRTVNLGRIRAGESTNKV
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECEEECCCCCCCC
PDVAEGWFNIRVTEHDDPGALIDKIRKTVSGTVSIVRTVPVFLAADSPYTERLLALSGAT
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCC
AGKAHGASDARYLGENGLTGVVWGAEGFNTLHSRDECLHIPSLQSIYDPLMQLAREMEEH
CCCCCCCCCHHHHCCCCCEEEEECCCCCHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHH
AAV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA