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The map label for this gene is kinE [H]

Identifier: 78356212

GI number: 78356212

Start: 1187881

End: 1190058

Strand: Direct

Name: kinE [H]

Synonym: Dde_1165

Alternate gene names: 78356212

Gene position: 1187881-1190058 (Clockwise)

Preceding gene: 78356211

Following gene: 78356216

Centisome position: 31.84

GC content: 59.78

Gene sequence:

>2178_bases
ATGATGCAGGATTCCAGAAAAAAACAGCCCGATCTTCCGCCGGACAGTCAGGGGGGTGAGCACCCGCTCCGTGCCGGACA
GGCTATTTCTTCCGGAGCGGCAGATGCAGCGTCCGGAGGGTCTTCTGCGCCGCAGGAAGACCTGCAGTACCGTGATGCGG
TGAGCAGCCTGATAGGGTTGGGCGAAAAGTCGCTGCGCAAGAGTTATTATCCTGAACTGAGGCGCAAGATTGCTGAGCTG
GATGTGTTCCGGCGCATGGTTGACCACGCCAGCGATGCCATGCTGCTGCTGCGGACAGGCCCGCCTCTTGCCGTCACGCA
TCTGAATTCTGCCGCGCGCCGTCTGCTGGGGCCGGGACCGGAGGAGGGCGGCAGCATTGATCTGGCGGCGCAGCTGGGCC
CCCGTGTGGAAGCCGCGTTGCTGGCAAGCAGCAAGGGGGGGCGTTCCAAACGCCTGACGCTGCATGTGCAGCAGGCGGAC
GGAGCCGAACTGGTGCTCGAAGCCTCGTTTTCGCCCATGCCGTCGGCGGAGGGCGATCCGGGGCAGGTAATCATGGTGGC
CCGTGATGTGACAGCGGGGTTCAGGGCCGAACGTTCGCTGCGCGAGAGCGAGGAACGCTTCCGCGTGGTCTTTGAGCAGT
CCATGCAGCATGTGGTTGTCCTTTCTCCCGAAGGTCTTATTCTGCGCGCCAACCAGAGTCTGCTTTCCGAATTTGCCATC
TCTTTTGACGACCTGCGCGGCCAGCGTCTGAGTGATGCTCCGTGGCTGCGCAATATGCCGGAAATGGCGCGCAATGCCGA
ATCGGATGTAAGCAGTGCATCGGCGGGTGTCAGCGTGCGCCGGCTGTATCCTGTGCGGCTGCCATCGGGGCAGCTGTTTT
TCGACTGCTCTTTCAAACCGGTGCGTGACGATGCCGGACGTATTATCCATGTGGTTGTGGAGGCACGCGATATTACCGCG
CTGCTGGAGGCAGAGAATGAATCGCGCATGCTGCGCGGGCTGCTGACCAATGTGGTGGATTCCATGCCTTCGGTGCTGGC
TGCGGTTGACGCCGCGGGCAGGGTCATCCAGTGGAACAGAGAGGCCGAACGGCTGACCGGAACATCTGCACGCGAGGCCA
TGGGCCGTCCGGTGGAGGCCATGCTGCCGCATGTGGCTTCGCTGCGTTCGATGGTGCGCTACAGCATGGCCGAACAGCGC
CCCATGGCCGAGTCTAAGGTGCCGCGGACGCTGCCCGGCGGAAGGGTCGTTTATGAGGATGTGACAGTGTATCCGTTGTC
CACGGGACATGATCAGGGCGCTGTCATCCGCATTGACGATGTTTCGGACAAAGTGCTGATGGAACAGACCATGGTGCAGG
CGGAAAAGATGCTTTCCGTCGGCGGTCTTGCCGCAGGTATGGCTCACGAAATCAACAACCCTCTGGGTGCTGTTGTGCAG
GGGCTGCAAAATGTGTTTCGCCGTCTTGATCCTGCGGTTGACCGTAACAGCACTGTGGCGGCCGCGCTGGGGTGCGATCT
GCATGCCATGGATGCATACATGCGCGAGCGGAAAATATACCGTATTCTTGAAGGAATGCGTGAAGCCTCCATGCGGGCGG
CAGATATTGTCCGCAATATGCTCACCTTTTCCCGCAAGTCGGATATGTCGCGCTCTTCGTGCGATCTGCACGCCATGATG
GACAGTGTCATTTCACTGGCAGAAGCTGACTACGATCTGAAAAAACATTACGACTTCAAAAGAATAAAGCTGGTCCGGCT
GTACGAGCCCGTACCGCTTATCGCCTGTAATAAAACCGAGGTGGAGCAGGTGCTGCTGAACCTGCTGCGTAATGCGGCCC
AGTCGTTTTATGCCGTGGGCACCATTGCCGGTTCCGGTACAAAACGGGTGCTGTTGTGCCATGAACCGGTGATAACGGTG
CGGATACGCAAAGCGGAGCGCTACGTGTATGTGGACGTGGAGGATAACGGACCGGGTATGCCGGAAGATGTGACCCTGCG
CGTGTTCGAGCCGTTTTTCACAACCAAGGCACCGGGCGCGGGCACGGGGCTGGGGTTGTCCGTTTCTTATTTTATCATCA
CCCGAAATCATAAAGGCACCATGACGGTGACATCGGCGCCGGGCAGAGGCAGCTGTTTTACCGTGGGGCTGCCGCATAAC
GGCAGCACGCCGGAGTGA

Upstream 100 bases:

>100_bases
GCACGATGCCTGCATGCTGACCAACCCCCGCAGGTTTACGCTGCAGGAAGTGGAAGCATTGTATCTGGAAGCGTTTTGAA
AATGAAATCAAGGGTGCGGC

Downstream 100 bases:

>100_bases
ATTGAGCGGTCCGTCACCGGCGCTCAGCGTGAGGCACTGTCGTATCTGCGGTTGCGTTTGCAGCGGTACATGGCGTGGTC
GGCGTGGCTGATAATCTGGT

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 725; Mature: 725

Protein sequence:

>725_residues
MMQDSRKKQPDLPPDSQGGEHPLRAGQAISSGAADAASGGSSAPQEDLQYRDAVSSLIGLGEKSLRKSYYPELRRKIAEL
DVFRRMVDHASDAMLLLRTGPPLAVTHLNSAARRLLGPGPEEGGSIDLAAQLGPRVEAALLASSKGGRSKRLTLHVQQAD
GAELVLEASFSPMPSAEGDPGQVIMVARDVTAGFRAERSLRESEERFRVVFEQSMQHVVVLSPEGLILRANQSLLSEFAI
SFDDLRGQRLSDAPWLRNMPEMARNAESDVSSASAGVSVRRLYPVRLPSGQLFFDCSFKPVRDDAGRIIHVVVEARDITA
LLEAENESRMLRGLLTNVVDSMPSVLAAVDAAGRVIQWNREAERLTGTSAREAMGRPVEAMLPHVASLRSMVRYSMAEQR
PMAESKVPRTLPGGRVVYEDVTVYPLSTGHDQGAVIRIDDVSDKVLMEQTMVQAEKMLSVGGLAAGMAHEINNPLGAVVQ
GLQNVFRRLDPAVDRNSTVAAALGCDLHAMDAYMRERKIYRILEGMREASMRAADIVRNMLTFSRKSDMSRSSCDLHAMM
DSVISLAEADYDLKKHYDFKRIKLVRLYEPVPLIACNKTEVEQVLLNLLRNAAQSFYAVGTIAGSGTKRVLLCHEPVITV
RIRKAERYVYVDVEDNGPGMPEDVTLRVFEPFFTTKAPGAGTGLGLSVSYFIITRNHKGTMTVTSAPGRGSCFTVGLPHN
GSTPE

Sequences:

>Translated_725_residues
MMQDSRKKQPDLPPDSQGGEHPLRAGQAISSGAADAASGGSSAPQEDLQYRDAVSSLIGLGEKSLRKSYYPELRRKIAEL
DVFRRMVDHASDAMLLLRTGPPLAVTHLNSAARRLLGPGPEEGGSIDLAAQLGPRVEAALLASSKGGRSKRLTLHVQQAD
GAELVLEASFSPMPSAEGDPGQVIMVARDVTAGFRAERSLRESEERFRVVFEQSMQHVVVLSPEGLILRANQSLLSEFAI
SFDDLRGQRLSDAPWLRNMPEMARNAESDVSSASAGVSVRRLYPVRLPSGQLFFDCSFKPVRDDAGRIIHVVVEARDITA
LLEAENESRMLRGLLTNVVDSMPSVLAAVDAAGRVIQWNREAERLTGTSAREAMGRPVEAMLPHVASLRSMVRYSMAEQR
PMAESKVPRTLPGGRVVYEDVTVYPLSTGHDQGAVIRIDDVSDKVLMEQTMVQAEKMLSVGGLAAGMAHEINNPLGAVVQ
GLQNVFRRLDPAVDRNSTVAAALGCDLHAMDAYMRERKIYRILEGMREASMRAADIVRNMLTFSRKSDMSRSSCDLHAMM
DSVISLAEADYDLKKHYDFKRIKLVRLYEPVPLIACNKTEVEQVLLNLLRNAAQSFYAVGTIAGSGTKRVLLCHEPVITV
RIRKAERYVYVDVEDNGPGMPEDVTLRVFEPFFTTKAPGAGTGLGLSVSYFIITRNHKGTMTVTSAPGRGSCFTVGLPHN
GSTPE
>Mature_725_residues
MMQDSRKKQPDLPPDSQGGEHPLRAGQAISSGAADAASGGSSAPQEDLQYRDAVSSLIGLGEKSLRKSYYPELRRKIAEL
DVFRRMVDHASDAMLLLRTGPPLAVTHLNSAARRLLGPGPEEGGSIDLAAQLGPRVEAALLASSKGGRSKRLTLHVQQAD
GAELVLEASFSPMPSAEGDPGQVIMVARDVTAGFRAERSLRESEERFRVVFEQSMQHVVVLSPEGLILRANQSLLSEFAI
SFDDLRGQRLSDAPWLRNMPEMARNAESDVSSASAGVSVRRLYPVRLPSGQLFFDCSFKPVRDDAGRIIHVVVEARDITA
LLEAENESRMLRGLLTNVVDSMPSVLAAVDAAGRVIQWNREAERLTGTSAREAMGRPVEAMLPHVASLRSMVRYSMAEQR
PMAESKVPRTLPGGRVVYEDVTVYPLSTGHDQGAVIRIDDVSDKVLMEQTMVQAEKMLSVGGLAAGMAHEINNPLGAVVQ
GLQNVFRRLDPAVDRNSTVAAALGCDLHAMDAYMRERKIYRILEGMREASMRAADIVRNMLTFSRKSDMSRSSCDLHAMM
DSVISLAEADYDLKKHYDFKRIKLVRLYEPVPLIACNKTEVEQVLLNLLRNAAQSFYAVGTIAGSGTKRVLLCHEPVITV
RIRKAERYVYVDVEDNGPGMPEDVTLRVFEPFFTTKAPGAGTGLGLSVSYFIITRNHKGTMTVTSAPGRGSCFTVGLPHN
GSTPE

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=417, Percent_Identity=26.8585131894484, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI1790436, Length=276, Percent_Identity=30.7971014492754, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790300, Length=387, Percent_Identity=24.8062015503876, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 79164; Mature: 79164

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQDSRKKQPDLPPDSQGGEHPLRAGQAISSGAADAASGGSSAPQEDLQYRDAVSSLIGL
CCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
GEKSLRKSYYPELRRKIAELDVFRRMVDHASDAMLLLRTGPPLAVTHLNSAARRLLGPGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHCCCCC
EEGGSIDLAAQLGPRVEAALLASSKGGRSKRLTLHVQQADGAELVLEASFSPMPSAEGDP
CCCCCEEEHHHCCCCHHHHHEECCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCC
GQVIMVARDVTAGFRAERSLRESEERFRVVFEQSMQHVVVLSPEGLILRANQSLLSEFAI
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHHHH
SFDDLRGQRLSDAPWLRNMPEMARNAESDVSSASAGVSVRRLYPVRLPSGQLFFDCSFKP
HHHHHCCCCCCCCCHHCCCHHHHHCCHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCC
VRDDAGRIIHVVVEARDITALLEAENESRMLRGLLTNVVDSMPSVLAAVDAAGRVIQWNR
CCCCCCCEEEEEEEHHHHEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
EAERLTGTSAREAMGRPVEAMLPHVASLRSMVRYSMAEQRPMAESKVPRTLPGGRVVYED
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEEC
VTVYPLSTGHDQGAVIRIDDVSDKVLMEQTMVQAEKMLSVGGLAAGMAHEINNPLGAVVQ
EEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHH
GLQNVFRRLDPAVDRNSTVAAALGCDLHAMDAYMRERKIYRILEGMREASMRAADIVRNM
HHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTFSRKSDMSRSSCDLHAMMDSVISLAEADYDLKKHYDFKRIKLVRLYEPVPLIACNKTE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHEEEEEEECCCCEEEECHHH
VEQVLLNLLRNAAQSFYAVGTIAGSGTKRVLLCHEPVITVRIRKAERYVYVDVEDNGPGM
HHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCEEEEEEEECCEEEEEEEECCCCCC
PEDVTLRVFEPFFTTKAPGAGTGLGLSVSYFIITRNHKGTMTVTSAPGRGSCFTVGLPHN
CCCCEEEEECHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCEEEEECCCC
GSTPE
CCCCC
>Mature Secondary Structure
MMQDSRKKQPDLPPDSQGGEHPLRAGQAISSGAADAASGGSSAPQEDLQYRDAVSSLIGL
CCCCCCCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
GEKSLRKSYYPELRRKIAELDVFRRMVDHASDAMLLLRTGPPLAVTHLNSAARRLLGPGP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCHHHHHHCCCCC
EEGGSIDLAAQLGPRVEAALLASSKGGRSKRLTLHVQQADGAELVLEASFSPMPSAEGDP
CCCCCEEEHHHCCCCHHHHHEECCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCC
GQVIMVARDVTAGFRAERSLRESEERFRVVFEQSMQHVVVLSPEGLILRANQSLLSEFAI
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHHHHH
SFDDLRGQRLSDAPWLRNMPEMARNAESDVSSASAGVSVRRLYPVRLPSGQLFFDCSFKP
HHHHHCCCCCCCCCHHCCCHHHHHCCHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCC
VRDDAGRIIHVVVEARDITALLEAENESRMLRGLLTNVVDSMPSVLAAVDAAGRVIQWNR
CCCCCCCEEEEEEEHHHHEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
EAERLTGTSAREAMGRPVEAMLPHVASLRSMVRYSMAEQRPMAESKVPRTLPGGRVVYED
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEEEEC
VTVYPLSTGHDQGAVIRIDDVSDKVLMEQTMVQAEKMLSVGGLAAGMAHEINNPLGAVVQ
EEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHH
GLQNVFRRLDPAVDRNSTVAAALGCDLHAMDAYMRERKIYRILEGMREASMRAADIVRNM
HHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTFSRKSDMSRSSCDLHAMMDSVISLAEADYDLKKHYDFKRIKLVRLYEPVPLIACNKTE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCHHHEEEEEEECCCCEEEECHHH
VEQVLLNLLRNAAQSFYAVGTIAGSGTKRVLLCHEPVITVRIRKAERYVYVDVEDNGPGM
HHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCEEEEEEEECCEEEEEEEECCCCCC
PEDVTLRVFEPFFTTKAPGAGTGLGLSVSYFIITRNHKGTMTVTSAPGRGSCFTVGLPHN
CCCCEEEEECHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCEEEEECCCC
GSTPE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]