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The map label for this gene is phsB [H]

Identifier: 78356122

GI number: 78356122

Start: 1102776

End: 1103222

Strand: Reverse

Name: phsB [H]

Synonym: Dde_1075

Alternate gene names: 78356122

Gene position: 1103222-1102776 (Counterclockwise)

Preceding gene: 78356123

Following gene: 78356121

Centisome position: 29.58

GC content: 55.7

Gene sequence:

>447_bases
ATGAGCAGATACAGACTGAAACTGAACAAAAAACGTTGCATCCACTGCAAGGCATGTGAAGTGCATTGCAAAGCGAAAAA
CAACACTCCGGCAGGCATCAAGCTGGGCGTGCACACATCTTCCGAACCGGTGATGGTGGACGGCGTACCCCGCATGAAAG
CTGCCTATCAGTGCTGCTACCACTGCGAAACGCCCGAATGCGTGGACGTATGCCCCACCGGCGCCATGATACGGCGCGAA
TCTGACGGTATAGTATATGTTGATCAGGAAACTTGCATCGGATGCGAAGCCTGCATCGATGCCTGCCCATGGCATATTCC
CGTCTTCAACGCCGCCACAGCCACTGTACTCAAATGCGACTTCTGCATGGACCGCGTTGACGAGGGCAAGCTGCCCGCCT
GTGTCACCGCCTGCACCACGCACGCACTGAGCTTCACCCGCAAATAG

Upstream 100 bases:

>100_bases
TGCCCGGCGGACTGGAGCGCTACGATACATCCGGCGGCGGTCTTTCACTTCAGGAGCACTTCGTGGTTATCAGCAAGGCA
GAGCAGGACGGGGGGGCAGC

Downstream 100 bases:

>100_bases
CCGGCAAGGGGTACTGCGCGGGCGCCCTGCCCGCGCAGTACCCCTTGCCATACCAGCCGTCTGCCCTTTGCCCGCCTTCT
CTTTTCCGCACTGTTACTGG

Product: iron-sulfur cluster-binding protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSRYRLKLNKKRCIHCKACEVHCKAKNNTPAGIKLGVHTSSEPVMVDGVPRMKAAYQCCYHCETPECVDVCPTGAMIRRE
SDGIVYVDQETCIGCEACIDACPWHIPVFNAATATVLKCDFCMDRVDEGKLPACVTACTTHALSFTRK

Sequences:

>Translated_148_residues
MSRYRLKLNKKRCIHCKACEVHCKAKNNTPAGIKLGVHTSSEPVMVDGVPRMKAAYQCCYHCETPECVDVCPTGAMIRRE
SDGIVYVDQETCIGCEACIDACPWHIPVFNAATATVLKCDFCMDRVDEGKLPACVTACTTHALSFTRK
>Mature_147_residues
SRYRLKLNKKRCIHCKACEVHCKAKNNTPAGIKLGVHTSSEPVMVDGVPRMKAAYQCCYHCETPECVDVCPTGAMIRRES
DGIVYVDQETCIGCEACIDACPWHIPVFNAATATVLKCDFCMDRVDEGKLPACVTACTTHALSFTRK

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=144, Percent_Identity=40.9722222222222, Blast_Score=106, Evalue=5e-25,
Organism=Escherichia coli, GI1787122, Length=151, Percent_Identity=41.0596026490066, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI226510944, Length=149, Percent_Identity=41.6107382550336, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI1787872, Length=151, Percent_Identity=38.4105960264901, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787749, Length=151, Percent_Identity=36.4238410596026, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1790326, Length=151, Percent_Identity=35.0993377483444, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1787478, Length=114, Percent_Identity=35.0877192982456, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1787740, Length=119, Percent_Identity=32.7731092436975, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1789370, Length=93, Percent_Identity=38.7096774193548, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI87082179, Length=150, Percent_Identity=32.6666666666667, Blast_Score=70, Evalue=7e-14,
Organism=Escherichia coli, GI1787963, Length=149, Percent_Identity=28.1879194630872, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1789067, Length=166, Percent_Identity=34.3373493975904, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1788811, Length=152, Percent_Identity=32.8947368421053, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI2367245, Length=149, Percent_Identity=34.8993288590604, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 16335; Mature: 16204

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
14.9 %Cys+Met (Translated Protein)
11.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
14.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYRLKLNKKRCIHCKACEVHCKAKNNTPAGIKLGVHTSSEPVMVDGVPRMKAAYQCCY
CCCEEEEECCCEEEEEEEEEEEEEECCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHH
HCETPECVDVCPTGAMIRRESDGIVYVDQETCIGCEACIDACPWHIPVFNAATATVLKCD
CCCCCCCEEECCCCCEEEECCCCEEEECCCCCCCHHHHHHCCCCCCEEECCCHHHEEEHH
FCMDRVDEGKLPACVTACTTHALSFTRK
HHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRYRLKLNKKRCIHCKACEVHCKAKNNTPAGIKLGVHTSSEPVMVDGVPRMKAAYQCCY
CCEEEEECCCEEEEEEEEEEEEEECCCCCCEEEEEEECCCCCEEEECCCHHHHHHHHHH
HCETPECVDVCPTGAMIRRESDGIVYVDQETCIGCEACIDACPWHIPVFNAATATVLKCD
CCCCCCCEEECCCCCEEEECCCCEEEECCCCCCCHHHHHHCCCCCCEEECCCHHHEEEHH
FCMDRVDEGKLPACVTACTTHALSFTRK
HHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]