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The map label for this gene is phsA [H]

Identifier: 78356123

GI number: 78356123

Start: 1103219

End: 1105345

Strand: Reverse

Name: phsA [H]

Synonym: Dde_1076

Alternate gene names: 78356123

Gene position: 1105345-1103219 (Counterclockwise)

Preceding gene: 78356126

Following gene: 78356122

Centisome position: 29.63

GC content: 61.73

Gene sequence:

>2127_bases
ATGGCAGAAGACAAGACCGTTTTCAGCGTCTGCGGCATGTGCAGTGTGCGCTGCCCCATCATGGTGGAGACTCGCGGCGG
CAACGCCGCATGGATACAGGGTAACCCTTATTCAGCACTCAAAGGGGCGCTCTGTGCCCGCGGGGCTGCAGGCATGGCGC
TGGAATACGATTCCGAGCGTCCGGTCACGCCCCTTATCAGAACAGGAGAACGGGGCGAAGGAAAGTGGCGTGAAGCCACT
TGGGACGAGGCACTGGACTATGTGGCCGCCAGACTGAAAAACATCATGGCGGAACACGGCGGCAGAAGCGTTCTGTGGAG
CGACCGCGGCGGTCCCTTTGCCGACCTGCATCAGGCGTTTGTGCGCGGACTCGGCTCGCCCAATTACTGTAACCACGACG
CCTCGTGCGCCCGCAACGTGCAGCACGCCGCCCAGTCCGTCATGGGCATGGGGCGCAAGCAGGTTGCCTACGACCTGCGT
AACGCACGCCACATTGTGCTGCAGACCCGCAATATTCTCGAAGCCATCAACGTGGCCGAATGCAACGGTGTCATGGACGC
GCTGGACAACGGCTGCAAGCTGAGCGTCATCGATATACGCGCCTCTGTCAGCGCCAGCAAAGCCGACAACTTTTTCATGG
TGCGTCCGGGCACCGACTACGCCTTCAATCTGGCTGTGATCAATCAGCTGATCACCCGCGGGCTGTACGACAAGGAGTAC
GTGGCCCGCCATTTTCATGACTTTGACGAACTGGCGGCGTTCACCGCGCCCTGTACGGCAGCATGGGCGCAGGAACAGAC
AGGTGTACCCGCGGAACGCATCGAGGCGCTGGCCGCGCAGCTGGCAGAAGCAGCCCCTTCGGTCATCTGGCACCCCGGCT
GGATGGTGGCCCGCTACGATGATTCATTCTATGTATGCCGCACGGCCTACATCATCAACGCGCTGCTCGGCTCCATAGGT
GCCAGGGGCGGTCTGCCGCTGGTCAGCACGCCCAAAGACGTGGGCCGCAAAGGACTGAACAAGCTGGCGGCACTGATGCC
GAAACCCGAAGAAAAACGCGCCGACGGCGTAGGCTGGCGGCACCCGCAGTTCGATGCGGGCCCGGGGCTGGTCAATCTGG
CGTACGACGCCATCGCCACAGCCGACCCCTATGCGGTCAAAGCCTACATATGCTACCGCCACGACCCGCTTATGGCCATG
CCCGACCCCGAAGCGCTCAAGCGCAAATGGGAAGGGCTTGACCTGCTTGTCAGTGTCACCTTCAGCTGGTCGGACACGGC
ATGGCACTCCGATGTGGTGCTGCCCCTTTCGCCCTACCTTTCACGCGAAAGCATCATAGCAGGCAAGGCCGGACTCAAGC
CGCAGTTTTTTGTGCGCAAACGGGCCATGCAGCCCACTGCAGACACCCGTGCAGACTGGGAAATTCTGTGCGGGCTGGCA
TCGCGCCTTACCGACGTGTGCCCCGCCATGAAGCATCTGGCGTTCACCCGTATAGAAGACATCTGGGACTATCAGCTGCG
CGACACGGGGGTAAGCATAGGCGATTTTGACGCCAAAGGATTTGTCGAACTGGCCGATACCCCCCTGTACCGCACGCCCG
AATCCTTTCCCACCGCCAGCGGCAAACTGGAGATGGTAAGCGCCAAATGGGCAAAAGCCGACATTCATACCCTGAAACCC
TACACAGCTCCGGCTGCGCCTCCGCAGGGGGCCTTCCGCATAGCCTTCGGCAGAGTGGGAGTGCACACGCAGGGGCACAC
CGTAAACAACCCGCTGCTGCATGAGCAGATGCCCGAAAATGTGGCATGGATACACACTTCACGTGCGGAACGACTGGGCA
TATGCACCGGAGACATGATAGAAGTGGCGCCTGCCGGCGCTCCCCGCAATCGCGCCGGACGCATGAAGGCCTTTGTGACA
GACTGCGTGCACCCCGAAGCACTGTTCATGGTGCACGGCTTCGGCCACAGGCTGCCTGTGGAATCGCGCGCCCGGGGATG
CGGCGTGGCCGACCACGAACTGATGCCCGGCGGACTGGAGCGCTACGATACATCCGGCGGCGGTCTTTCACTTCAGGAGC
ACTTCGTGGTTATCAGCAAGGCAGAGCAGGACGGGGGGGCAGCATGA

Upstream 100 bases:

>100_bases
CACAATCAGGCTGCGGCTTTTCAATTTTGCCCGTATGGCGTATTTTACCTGATTGACCGGCATCAGGTACGGCAGGTTCT
CAACCGTTGCGGAGGGACGA

Downstream 100 bases:

>100_bases
GCAGATACAGACTGAAACTGAACAAAAAACGTTGCATCCACTGCAAGGCATGTGAAGTGCATTGCAAAGCGAAAAACAAC
ACTCCGGCAGGCATCAAGCT

Product: thiosulfate reductase

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MAEDKTVFSVCGMCSVRCPIMVETRGGNAAWIQGNPYSALKGALCARGAAGMALEYDSERPVTPLIRTGERGEGKWREAT
WDEALDYVAARLKNIMAEHGGRSVLWSDRGGPFADLHQAFVRGLGSPNYCNHDASCARNVQHAAQSVMGMGRKQVAYDLR
NARHIVLQTRNILEAINVAECNGVMDALDNGCKLSVIDIRASVSASKADNFFMVRPGTDYAFNLAVINQLITRGLYDKEY
VARHFHDFDELAAFTAPCTAAWAQEQTGVPAERIEALAAQLAEAAPSVIWHPGWMVARYDDSFYVCRTAYIINALLGSIG
ARGGLPLVSTPKDVGRKGLNKLAALMPKPEEKRADGVGWRHPQFDAGPGLVNLAYDAIATADPYAVKAYICYRHDPLMAM
PDPEALKRKWEGLDLLVSVTFSWSDTAWHSDVVLPLSPYLSRESIIAGKAGLKPQFFVRKRAMQPTADTRADWEILCGLA
SRLTDVCPAMKHLAFTRIEDIWDYQLRDTGVSIGDFDAKGFVELADTPLYRTPESFPTASGKLEMVSAKWAKADIHTLKP
YTAPAAPPQGAFRIAFGRVGVHTQGHTVNNPLLHEQMPENVAWIHTSRAERLGICTGDMIEVAPAGAPRNRAGRMKAFVT
DCVHPEALFMVHGFGHRLPVESRARGCGVADHELMPGGLERYDTSGGGLSLQEHFVVISKAEQDGGAA

Sequences:

>Translated_708_residues
MAEDKTVFSVCGMCSVRCPIMVETRGGNAAWIQGNPYSALKGALCARGAAGMALEYDSERPVTPLIRTGERGEGKWREAT
WDEALDYVAARLKNIMAEHGGRSVLWSDRGGPFADLHQAFVRGLGSPNYCNHDASCARNVQHAAQSVMGMGRKQVAYDLR
NARHIVLQTRNILEAINVAECNGVMDALDNGCKLSVIDIRASVSASKADNFFMVRPGTDYAFNLAVINQLITRGLYDKEY
VARHFHDFDELAAFTAPCTAAWAQEQTGVPAERIEALAAQLAEAAPSVIWHPGWMVARYDDSFYVCRTAYIINALLGSIG
ARGGLPLVSTPKDVGRKGLNKLAALMPKPEEKRADGVGWRHPQFDAGPGLVNLAYDAIATADPYAVKAYICYRHDPLMAM
PDPEALKRKWEGLDLLVSVTFSWSDTAWHSDVVLPLSPYLSRESIIAGKAGLKPQFFVRKRAMQPTADTRADWEILCGLA
SRLTDVCPAMKHLAFTRIEDIWDYQLRDTGVSIGDFDAKGFVELADTPLYRTPESFPTASGKLEMVSAKWAKADIHTLKP
YTAPAAPPQGAFRIAFGRVGVHTQGHTVNNPLLHEQMPENVAWIHTSRAERLGICTGDMIEVAPAGAPRNRAGRMKAFVT
DCVHPEALFMVHGFGHRLPVESRARGCGVADHELMPGGLERYDTSGGGLSLQEHFVVISKAEQDGGAA
>Mature_707_residues
AEDKTVFSVCGMCSVRCPIMVETRGGNAAWIQGNPYSALKGALCARGAAGMALEYDSERPVTPLIRTGERGEGKWREATW
DEALDYVAARLKNIMAEHGGRSVLWSDRGGPFADLHQAFVRGLGSPNYCNHDASCARNVQHAAQSVMGMGRKQVAYDLRN
ARHIVLQTRNILEAINVAECNGVMDALDNGCKLSVIDIRASVSASKADNFFMVRPGTDYAFNLAVINQLITRGLYDKEYV
ARHFHDFDELAAFTAPCTAAWAQEQTGVPAERIEALAAQLAEAAPSVIWHPGWMVARYDDSFYVCRTAYIINALLGSIGA
RGGLPLVSTPKDVGRKGLNKLAALMPKPEEKRADGVGWRHPQFDAGPGLVNLAYDAIATADPYAVKAYICYRHDPLMAMP
DPEALKRKWEGLDLLVSVTFSWSDTAWHSDVVLPLSPYLSRESIIAGKAGLKPQFFVRKRAMQPTADTRADWEILCGLAS
RLTDVCPAMKHLAFTRIEDIWDYQLRDTGVSIGDFDAKGFVELADTPLYRTPESFPTASGKLEMVSAKWAKADIHTLKPY
TAPAAPPQGAFRIAFGRVGVHTQGHTVNNPLLHEQMPENVAWIHTSRAERLGICTGDMIEVAPAGAPRNRAGRMKAFVTD
CVHPEALFMVHGFGHRLPVESRARGCGVADHELMPGGLERYDTSGGGLSLQEHFVVISKAEQDGGAA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=586, Percent_Identity=24.5733788395904, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787870, Length=753, Percent_Identity=22.0451527224436, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI171474008, Length=697, Percent_Identity=23.3859397417504, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87081797, Length=712, Percent_Identity=21.4887640449438, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1787231, Length=557, Percent_Identity=22.8007181328546, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI3868720, Length=257, Percent_Identity=28.4046692607004, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 77305; Mature: 77174

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDKTVFSVCGMCSVRCPIMVETRGGNAAWIQGNPYSALKGALCARGAAGMALEYDSER
CCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCC
PVTPLIRTGERGEGKWREATWDEALDYVAARLKNIMAEHGGRSVLWSDRGGPFADLHQAF
CCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
VRGLGSPNYCNHDASCARNVQHAAQSVMGMGRKQVAYDLRNARHIVLQTRNILEAINVAE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHH
CNGVMDALDNGCKLSVIDIRASVSASKADNFFMVRPGTDYAFNLAVINQLITRGLYDKEY
HHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCHHH
VARHFHDFDELAAFTAPCTAAWAQEQTGVPAERIEALAAQLAEAAPSVIWHPGWMVARYD
HHHHHCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEEC
DSFYVCRTAYIINALLGSIGARGGLPLVSTPKDVGRKGLNKLAALMPKPEEKRADGVGWR
CCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
HPQFDAGPGLVNLAYDAIATADPYAVKAYICYRHDPLMAMPDPEALKRKWEGLDLLVSVT
CCCCCCCCCEEEHHHHHHHCCCCCEEEEEEEEECCCCEECCCHHHHHHHCCCCEEEEEEE
FSWSDTAWHSDVVLPLSPYLSRESIIAGKAGLKPQFFVRKRAMQPTADTRADWEILCGLA
EECCCCCCCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
SRLTDVCPAMKHLAFTRIEDIWDYQLRDTGVSIGDFDAKGFVELADTPLYRTPESFPTAS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
GKLEMVSAKWAKADIHTLKPYTAPAAPPQGAFRIAFGRVGVHTQGHTVNNPLLHEQMPEN
CCEEEEECHHHHCCHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHCCCC
VAWIHTSRAERLGICTGDMIEVAPAGAPRNRAGRMKAFVTDCVHPEALFMVHGFGHRLPV
EEEEECCCCCCEECCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
ESRARGCGVADHELMPGGLERYDTSGGGLSLQEHFVVISKAEQDGGAA
HHHHCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
AEDKTVFSVCGMCSVRCPIMVETRGGNAAWIQGNPYSALKGALCARGAAGMALEYDSER
CCCHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCCCEEEEECCCC
PVTPLIRTGERGEGKWREATWDEALDYVAARLKNIMAEHGGRSVLWSDRGGPFADLHQAF
CCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
VRGLGSPNYCNHDASCARNVQHAAQSVMGMGRKQVAYDLRNARHIVLQTRNILEAINVAE
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEEHHHHHHHHHHHH
CNGVMDALDNGCKLSVIDIRASVSASKADNFFMVRPGTDYAFNLAVINQLITRGLYDKEY
HHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCHHH
VARHFHDFDELAAFTAPCTAAWAQEQTGVPAERIEALAAQLAEAAPSVIWHPGWMVARYD
HHHHHCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCEEEEEEC
DSFYVCRTAYIINALLGSIGARGGLPLVSTPKDVGRKGLNKLAALMPKPEEKRADGVGWR
CCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
HPQFDAGPGLVNLAYDAIATADPYAVKAYICYRHDPLMAMPDPEALKRKWEGLDLLVSVT
CCCCCCCCCEEEHHHHHHHCCCCCEEEEEEEEECCCCEECCCHHHHHHHCCCCEEEEEEE
FSWSDTAWHSDVVLPLSPYLSRESIIAGKAGLKPQFFVRKRAMQPTADTRADWEILCGLA
EECCCCCCCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHH
SRLTDVCPAMKHLAFTRIEDIWDYQLRDTGVSIGDFDAKGFVELADTPLYRTPESFPTAS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
GKLEMVSAKWAKADIHTLKPYTAPAAPPQGAFRIAFGRVGVHTQGHTVNNPLLHEQMPEN
CCEEEEECHHHHCCHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHCCCC
VAWIHTSRAERLGICTGDMIEVAPAGAPRNRAGRMKAFVTDCVHPEALFMVHGFGHRLPV
EEEEECCCCCCEECCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
ESRARGCGVADHELMPGGLERYDTSGGGLSLQEHFVVISKAEQDGGAA
HHHHCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.19 {3-pyridinecarboxaldehyde-NAD+}} 0.032 {NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.54 {deamino-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]