Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 78224530

GI number: 78224530

Start: 3753948

End: 3754883

Strand: Direct

Name: fmt

Synonym: Gmet_3339

Alternate gene names: 78224530

Gene position: 3753948-3754883 (Clockwise)

Preceding gene: 78224529

Following gene: 78224531

Centisome position: 93.91

GC content: 59.29

Gene sequence:

>936_bases
ATGGCCGGACTCAGCATCATCTTCATGGGAACCCCTGATTTCGCCTGTCCCACGCTCACGAGACTCATCGAGCGAGGCGA
GGATGTAATTGCCGTCGTGACCCAACCTGACCGCCCCAAGGGTCGTGGCCAGAAACTGGTGCCGCCTCCCGTGAAGGTCA
TTGCCGAGGAGCATGGCATTCCGGTCCTGCAGCCCCAGAAAGTCCGTGCGCCCGAGGTTGTCGCACAGATCAGGGAACTG
AATCCTGACCTGATTGTGGTGGTCGCCTTCGGCCAGATTCTCCCCCAGAGCCTCCTCGAAATCCCTCGGCATGGCTGCAT
CAACATTCACGCCTCACTCCTTCCACGCTACCGGGGGGCCGCTCCCATTAACTGGTGCCTCATCAACGGCGAGACCGAGA
CCGGCATAACCACCATGCAGATGGATGCGGGTCTCGACACGGGGGATATGCTTGTCAAACGGTCCATCTCCATTGGCCCG
GACGAGGACGCCCAGTCGCTCCACGACCGCCTCTCCCTCCTTGGGGCTGAAACCATCGACGAGACCCTGGACAGACTCCA
GGTAGGCACCCTTACTCGCGAAAAGCAGGATGACGCTCTTACCTGCTACGCTCCGATGCTGAAGAAGGAAGACGGCCTTA
TCGACTGGAAGCAGGAGCCGCAGCAAATTAAAAACCTCGTTCGCGGCTTCACTCCGTGGCCCGGAGCGTATACCACGCTG
GACGGCAAGACACTGAAGCTGTACAAGGTGTCGGTGGCCACTGAATGCGGCAAGCCCGGAGATATCATGGCTGTCGGAAA
AGACGGGATCATCGTCGGCTGCGGGTCAGGTAGCCTCCGAATCGAGGAACTGCAACTGGAAGGAAGGAAACGGCTATCGG
CTCAAGATTTCCTAGCAGGTTGCCGTCTGGAGCCAGGGATTCGGCTCGGTCATTAA

Upstream 100 bases:

>100_bases
CCTGGAAGGGATGCTCTTCGTGGATCACCTTTCTCCCCTGAAGCGGGAGATGTTCAAGAAAAAATACCGCCGCATGGTTG
AGGAAGGGTAGGATTACTGC

Downstream 100 bases:

>100_bases
AATGCCTGACAATACTCCTAAAAAACGTCTTTTCATTGTGCTCATGGGGGTCACTTGTCTCCTGATTGTCGGCGCAATCT
ACCTTCTCTGGTGGATTCCC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAGLSIIFMGTPDFACPTLTRLIERGEDVIAVVTQPDRPKGRGQKLVPPPVKVIAEEHGIPVLQPQKVRAPEVVAQIREL
NPDLIVVVAFGQILPQSLLEIPRHGCINIHASLLPRYRGAAPINWCLINGETETGITTMQMDAGLDTGDMLVKRSISIGP
DEDAQSLHDRLSLLGAETIDETLDRLQVGTLTREKQDDALTCYAPMLKKEDGLIDWKQEPQQIKNLVRGFTPWPGAYTTL
DGKTLKLYKVSVATECGKPGDIMAVGKDGIIVGCGSGSLRIEELQLEGRKRLSAQDFLAGCRLEPGIRLGH

Sequences:

>Translated_311_residues
MAGLSIIFMGTPDFACPTLTRLIERGEDVIAVVTQPDRPKGRGQKLVPPPVKVIAEEHGIPVLQPQKVRAPEVVAQIREL
NPDLIVVVAFGQILPQSLLEIPRHGCINIHASLLPRYRGAAPINWCLINGETETGITTMQMDAGLDTGDMLVKRSISIGP
DEDAQSLHDRLSLLGAETIDETLDRLQVGTLTREKQDDALTCYAPMLKKEDGLIDWKQEPQQIKNLVRGFTPWPGAYTTL
DGKTLKLYKVSVATECGKPGDIMAVGKDGIIVGCGSGSLRIEELQLEGRKRLSAQDFLAGCRLEPGIRLGH
>Mature_310_residues
AGLSIIFMGTPDFACPTLTRLIERGEDVIAVVTQPDRPKGRGQKLVPPPVKVIAEEHGIPVLQPQKVRAPEVVAQIRELN
PDLIVVVAFGQILPQSLLEIPRHGCINIHASLLPRYRGAAPINWCLINGETETGITTMQMDAGLDTGDMLVKRSISIGPD
EDAQSLHDRLSLLGAETIDETLDRLQVGTLTREKQDDALTCYAPMLKKEDGLIDWKQEPQQIKNLVRGFTPWPGAYTTLD
GKTLKLYKVSVATECGKPGDIMAVGKDGIIVGCGSGSLRIEELQLEGRKRLSAQDFLAGCRLEPGIRLGH

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=315, Percent_Identity=31.1111111111111, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI238814322, Length=312, Percent_Identity=28.8461538461538, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI164663775, Length=317, Percent_Identity=28.7066246056782, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=48.3870967741936, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1788589, Length=282, Percent_Identity=30.4964539007092, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI133930964, Length=259, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319458, Length=364, Percent_Identity=26.3736263736264, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45550868, Length=302, Percent_Identity=33.4437086092715, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28571984, Length=257, Percent_Identity=34.2412451361868, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24585660, Length=245, Percent_Identity=29.3877551020408, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582400, Length=124, Percent_Identity=33.0645161290323, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_GEOMG (Q39QC2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386277.1
- HSSP:   P77398
- ProteinModelPortal:   Q39QC2
- SMR:   Q39QC2
- STRING:   Q39QC2
- GeneID:   3740654
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3339
- NMPDR:   fig|269799.3.peg.3315
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q39QC2
- ProtClustDB:   CLSK827657
- BioCyc:   GMET269799:GMET_3339-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33906; Mature: 33775

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLSIIFMGTPDFACPTLTRLIERGEDVIAVVTQPDRPKGRGQKLVPPPVKVIAEEHGI
CCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVLQPQKVRAPEVVAQIRELNPDLIVVVAFGQILPQSLLEIPRHGCINIHASLLPRYRGA
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEHHHHCCHHCCC
APINWCLINGETETGITTMQMDAGLDTGDMLVKRSISIGPDEDAQSLHDRLSLLGAETID
CCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ETLDRLQVGTLTREKQDDALTCYAPMLKKEDGLIDWKQEPQQIKNLVRGFTPWPGAYTTL
HHHHHHHHHCCCCCCCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEE
DGKTLKLYKVSVATECGKPGDIMAVGKDGIIVGCGSGSLRIEELQLEGRKRLSAQDFLAG
CCCEEEEEEEEEECCCCCCCCEEEECCCCEEEEECCCCEEEEEEEHHHHHCCCHHHHHHC
CRLEPGIRLGH
CCCCCCCCCCC
>Mature Secondary Structure 
AGLSIIFMGTPDFACPTLTRLIERGEDVIAVVTQPDRPKGRGQKLVPPPVKVIAEEHGI
CCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCC
PVLQPQKVRAPEVVAQIRELNPDLIVVVAFGQILPQSLLEIPRHGCINIHASLLPRYRGA
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEHHHHCCHHCCC
APINWCLINGETETGITTMQMDAGLDTGDMLVKRSISIGPDEDAQSLHDRLSLLGAETID
CCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ETLDRLQVGTLTREKQDDALTCYAPMLKKEDGLIDWKQEPQQIKNLVRGFTPWPGAYTTL
HHHHHHHHHCCCCCCCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEE
DGKTLKLYKVSVATECGKPGDIMAVGKDGIIVGCGSGSLRIEELQLEGRKRLSAQDFLAG
CCCEEEEEEEEEECCCCCCCCEEEECCCCEEEEECCCCEEEEEEEHHHHHCCCHHHHHHC
CRLEPGIRLGH
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA